scholarly journals Morphological and Molecular Characterization of Exophiala polymorpha sp. nov. Isolated from Sporotrichoid Lymphocutaneous Lesions in a Patient with Myasthenia Gravis

2015 ◽  
Vol 53 (9) ◽  
pp. 2816-2822 ◽  
Author(s):  
Lee K. Yong ◽  
Nathan P. Wiederhold ◽  
Deanna A. Sutton ◽  
Marcelo Sandoval-Denis ◽  
Jonathan R. Lindner ◽  
...  
Keyword(s):  
Myasthenia Gravis ◽  
Molecular Characterization ◽  
Elongation Factor ◽  
Internal Transcribed Spacer Region ◽  
Translation Elongation ◽  
Content Type ◽  
Elongation Factor 1 Alpha ◽  
Morphological And Molecular Characterization

Exophialaspecies are capable of causing cutaneous and subcutaneous infections in immunocompromised patients. AnExophialaisolate was cultured from a biopsy specimen of a lesion on the forearm of a patient with myasthenia gravis. The patient also had lesions on the palm and distal aspects of the hand, which were successfully treated with a long-term course of itraconazole. A detailed morphological and molecular characterization of the isolate was undertaken. Phylogenetic analysis of the internal transcribed spacer region and portions of the β-tubulin and translation elongation factor 1-alpha genes indicated that the isolate was a novel species closely related to but genetically distinct from species within theExophiala spiniferaclade; the nameExophiala polymorphasp. nov. is proposed. Morphologically,E. polymorphamost closely resemblesE. xenobioticabut it differs in possessing phialides bearing prominent, wide collarettes, and it does not produce chlamydospores.

Amanita griseofusca: A new species of Amanita in section Vaginatae from Malam Jabba, Swat, Pakistan

Phytotaxa ◽  
2018 ◽  
Vol 364 (2) ◽  
pp. 181 ◽  
Author(s):  
MUNAZZA KIRAN ◽  
JUNAID KHAN ◽  
HASSAN SHER ◽  
DONALD H. PFISTER ◽  
ABDUL NASIR KHALID
Keyword(s):  
New Species ◽  
Elongation Factor ◽  
Molecular Data ◽  
Internal Transcribed Spacer Region ◽  
Translation Elongation ◽  
Elongation Factor 1 Alpha ◽  
Pileus Surface ◽  
Elongation Factor 1 ◽  
A New Species ◽  
Partial Translation

A new species, Amanita griseofusca in section Vaginatae is described and illustrated here from Pakistan. Distinguishing characters of the new species include medium-sized basidiomata, greyish brown pileus surface with white to beige, membranous volval remnants present as one (large) to a few (small) warts, close lamellae which are cream colored with a pink tone, striations one third of the total pileus radius, broadly ellipsoidal to ellipsoidal basidiospores and white loose saccate volva turning beige at maturity. Molecular data inferred from partial nuc rDNA internal transcribed spacer region (ITS), partial nuc rDNA larger subunit region (LSU) and partial translation elongation factor 1-alpha (tef1) confirms the novelty of the present taxon.

scholarly journals Bambusicolous Arthrinium Species in Guangdong Province, China

2020 ◽  
Vol 11 ◽  
Author(s):  
Indunil C. Senanayake ◽  
Jayarama D. Bhat ◽  
Ratchadawan Cheewangkoon ◽  
Ning Xie
Keyword(s):  
Large Subunit ◽  
Elongation Factor ◽  
Morphological Characteristics ◽  
Guangdong Province ◽  
Nuclear Ribosomal Dna ◽  
Internal Transcribed Spacer Region ◽  
Translation Elongation ◽  
Bambusicolous Fungi ◽  
Elongation Factor 1 Alpha ◽  
Elongation Factor 1

A survey of bambusicolous fungi in Bijiashan Mountain Park, Shenzhen, Guangdong Province, China, revealed several Arthrinium-like taxa from dead sheaths, twigs, and clumps of Bambusa species. Phylogenetic relationships were investigated based on morphology and combined analyses of the internal transcribed spacer region (ITS), large subunit nuclear ribosomal DNA (LSU), beta tubulin (β-tubulin), and translation elongation factor 1-alpha (tef 1-α) gene sequences. Based on morphological characteristics and phylogenetic data, Arthrinium acutiapicum sp. nov. and Arthrinium pseudorasikravindrae sp. nov. are introduced herein with descriptions and illustrations. Additionally, two new locality records of Arthrinium bambusae and Arthrinium guizhouense are described and illustrated.

scholarly journals Molecular characterization and phylogenetic analyses of Lophodermella needle pathogens (Rhytismataceae) on Pinus species in the USA and Europe

PeerJ ◽  
2021 ◽  
Vol 9 ◽  
pp. e11435
Author(s):  
Jessa P. Ata ◽  
Kelly S. Burns ◽  
Suzanne Marchetti ◽  
Isabel A. Munck ◽  
Ludwig Beenken ◽  
...  
Keyword(s):  
Phylogenetic Analyses ◽  
Ribosomal Subunit ◽  
Elongation Factor ◽  
Translation Elongation ◽  
Phenotypic Characters ◽  
Elongation Factor 1 Alpha ◽  
The Usa ◽  
Phylogenetic Resolution ◽  
Elongation Factor 1

Increasing prevalence of conifer needle pathogens globally have prompted further studies on pathogen identification and a better understanding of phylogenetic relationships among needle pathogens. Several Lophodermella species can be aggressive pathogens causing needle cast in natural pine forests in the USA and Europe. However, their relationships with other Rhytismataceae species have historically been based on similarities of only limited phenotypic characters. Currently, no molecular studies have been completed to elucidate their relationships with other Lophodermella needle pathogens. This study collected and sequenced three gene loci, namely: internal transcribed spacer, large ribosomal subunit, and translation elongation factor 1-alpha, from five Lophodermella needle pathogens from North America (L. arcuata, L. concolor, L. montivaga) and Europe (L. conjuncta and L. sulcigena) to distinguish phylogeny within Rhytismatacaeae, including Lophophacidium dooksii. Phylogenetic analyses of the three loci revealed that all but L. conjuncta that were sampled in this study consistently clustered in a well-supported clade within Rhytismataceae. The multi-gene phylogeny also confirmed consistent nesting of L. dooksii, a needle pathogen of Pinus strobus, within the clade. Potential synapomorphic characters such as ascomata position and ascospore shape for the distinct clade were also explored. Further, a rhytismataceous species on P. flexilis that was morphologically identified as L. arcuata was found to be unique based on the sequences at the three loci. This study suggests a potential wider range of host species within the genus and the need for genetic characterization of other Lophodermella and Lophophacidium species to provide a higher phylogenetic resolution.

Molecular Characterization of the cDNA Coding for Translation Elongation Factor-2 of Pathogenic Entamoeba histolytica

1993 ◽  
Vol 12 (1) ◽  
pp. 89-96 ◽  
Author(s):  
BARBARA PLAIMAUER ◽  
STEPHAN ORTNER ◽  
GERHARD WIEDERMANN ◽  
OTTO SCHEINER ◽  
MICHAEL DUCHÊNE
Keyword(s):  
Molecular Characterization ◽  
Entamoeba Histolytica ◽  
Elongation Factor ◽  
Translation Elongation Factor ◽  
Translation Elongation ◽  
Elongation Factor 2

Morphological and molecular characterization of human gastric mucous cells in long-term primary culture

1998 ◽  
Vol 436 (6) ◽  
pp. 871-881 ◽  
Author(s):  
S. Wagner ◽  
Marie-Luise Enss ◽  
Markus Cornberg ◽  
Heiko Mix ◽  
Silke Schumann ◽  
...  
Keyword(s):  
Primary Culture ◽  
Molecular Characterization ◽  
Mucous Cells ◽  
Gastric Mucous ◽  
Morphological And Molecular Characterization

scholarly journals Characterization of Phyllosticta hostae causing Phyllosticta leaf spot on spider lily in China

2015 ◽  
Vol 55 (4) ◽  
pp. 438-445 ◽  
Author(s):  
Run Hua Yi ◽  
Luo Jun Gan ◽  
Jing Chen ◽  
Xiao Ling Xu
Keyword(s):  
Leaf Spot ◽  
Elongation Factor ◽  
Morphological Characteristics ◽  
Leaf Spot Disease ◽  
Translation Elongation ◽  
Spot Disease ◽  
Its Gene ◽  
Elongation Factor 1 Alpha ◽  
Alpha Gene

Abstract Leaf spot disease on the spider lily [Hymenocallis littoralis (Jacq.) Salisb.] continues to cause serious problems in China. To confirm the pathogen, the pathogenicity of isolates from diseased leaves was tested according to Koch’s postulates. The isolates were tentatively identified using morphological characteristics and confirmation was done by phylogenetic analysis of the translation elongation factor 1-alpha gene (TEF1), the actin gene (ACT), and internal transcibed spacer (ITS) sequences using neighbor-joining (NJ), maximum parsimony (MP), and Bayesian inference (BI) methods. The pathogen was identified as Phyllosticta hostae. Molecular analysis indicated very little diversity in the TEF1, ACT, and ITS gene. This is the first report of P. hostae causing leaf spot disease on spider lily in China.

scholarly journals Unravelling Diaporthe Species Associated with Woody Hosts from Karst Formations (Guizhou) in China

2020 ◽  
Vol 6 (4) ◽  
pp. 251
Author(s):  
Asha J. Dissanayake ◽  
Ya-Ya Chen ◽  
Jian-Kui (Jack) Liu
Keyword(s):  
Phylogenetic Analysis ◽  
Elongation Factor ◽  
Nature Reserves ◽  
Guizhou Province ◽  
Internal Transcribed Spacer Region ◽  
Translation Elongation ◽  
Elongation Factor 1 Alpha ◽  
Molecular Phylogenetic ◽  
Culture Characteristics ◽  
Elongation Factor 1

Though several Diaporthe species have been reported in China, little is known about the species associated with nature reserves in Guizhou province. During a survey of fungi in six nature reserves in Guizhou province of China, thirty-one Diaporthe isolates were collected from different woody hosts. Based on morphology, culture characteristics and molecular phylogenetic analysis, these isolates were characterized and identified. Phylogenetic analysis of internal transcribed spacer region (ITS), combined with translation elongation factor 1-alpha (tef), β-tubulin (tub), calmodulin (cal) and histone H3 (his) gene regions identified five known Diaporthe species and seven distinct lineages representing novel Diaporthe species. The details of five known species: Diaporthe cercidis, D. cinnamomi, D. conica, D. nobilis and D. sackstonii are given and the seven new species D. constrictospora, D. ellipsospora, D. guttulata, D. irregularis, D. lenispora, D. minima, and D. minusculata are introduced with detailed descriptions and illustrations. This study revealed a high diversity of previously undescribed Diaporthe species associated with woody hosts in various nature reserves of Guizhou province, indicating that there is a potential of Diaporthe species remains to be discovered in this unique landform (Karst formations) in China. Interestingly, the five known Diaporthe species have been reported as pathogens of various hosts, and this could indicate that those newly introduced species in this study could be potentially pathogenic pending further studies to confirm.

scholarly journals Structural and Functional Characterization of the N Terminus of Schizosaccharomyces pombe Cwf10

2013 ◽  
Vol 12 (11) ◽  
pp. 1472-1489 ◽  
Author(s):  
S. Brent Livesay ◽  
Scott E. Collier ◽  
Danny A. Bitton ◽  
Jürg Bähler ◽  
Melanie D. Ohi
Keyword(s):  
Schizosaccharomyces Pombe ◽  
Functional Characterization ◽  
Elongation Factor ◽  
Mrna Splicing ◽  
Multiple Roles ◽  
Translation Elongation ◽  
Content Type ◽  
Eukaryotic Translation ◽  
Spliceosome Activation

ABSTRACT The spliceosome is a dynamic macromolecular machine that catalyzes the removal of introns from pre-mRNA, yielding mature message. Schizosaccharomyces pombe Cwf10 (homolog of Saccharomyces cerevisiae Snu114 and human U5-116K), an integral member of the U5 snRNP, is a GTPase that has multiple roles within the splicing cycle. Cwf10/Snu114 family members are highly homologous to eukaryotic translation elongation factor EF2, and they contain a conserved N-terminal extension (NTE) to the EF2-like portion, predicted to be an intrinsically unfolded domain. Using S. pombe as a model system, we show that the NTE is not essential, but cells lacking this domain are defective in pre-mRNA splicing. Genetic interactions between cwf10 -Δ NTE and other pre-mRNA splicing mutants are consistent with a role for the NTE in spliceosome activation and second-step catalysis. Characterization of Cwf10-NTE by various biophysical techniques shows that in solution the NTE contains regions of both structure and disorder. The first 23 highly conserved amino acids of the NTE are essential for its role in splicing but when overexpressed are not sufficient to restore pre-mRNA splicing to wild-type levels in cwf10 -Δ NTE cells. When the entire NTE is overexpressed in the cwf10 -Δ NTE background, it can complement the truncated Cwf10 protein in trans , and it immunoprecipitates a complex similar in composition to the late-stage U5.U2/U6 spliceosome. These data show that the structurally flexible NTE is capable of independently incorporating into the spliceosome and improving splicing function, possibly indicating a role for the NTE in stabilizing conformational rearrangements during a splice cycle.

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