scholarly journals Rapid Identification and Discrimination ofBrucellaIsolates by Use of Real-Time PCR and High-Resolution Melt Analysis

2010 ◽  
Vol 48 (3) ◽  
pp. 697-702 ◽  
Author(s):  
Jonas M. Winchell ◽  
Bernard J. Wolff ◽  
Rebekah Tiller ◽  
Michael D. Bowen ◽  
Alex R. Hoffmaster
Keyword(s):  
High Resolution ◽  
Real Time ◽  
Real Time Pcr ◽  
Rapid Identification ◽  
High Resolution Melt ◽  
High Resolution Melt Analysis

Development of multiplex real-time PCR and high-resolution melt analysis for detection of three viruses in penaeid shrimp

2010 ◽  
Vol 150 ◽  
pp. 127-127 ◽  
Author(s):  
Benjaporn Panichareon ◽  
Paisarn Khawsak ◽  
Warin Deesukon ◽  
Wasana Sukhumsirichart
Keyword(s):  
High Resolution ◽  
Real Time ◽  
Real Time Pcr ◽  
Penaeid Shrimp ◽  
High Resolution Melt ◽  
High Resolution Melt Analysis

scholarly journals Genotyping of Mycoplasma pneumoniae isolates using real-time PCR and high-resolution melt analysis

2009 ◽  
Vol 15 (8) ◽  
pp. 756-762 ◽  
Author(s):  
S.B. Schwartz ◽  
K.A. Thurman ◽  
S.L. Mitchell ◽  
B.J. Wolff ◽  
J.M. Winchell
Keyword(s):  
High Resolution ◽  
Real Time ◽  
Mycoplasma Pneumoniae ◽  
Real Time Pcr ◽  
High Resolution Melt ◽  
High Resolution Melt Analysis

scholarly journals Genotyping of Chlamydophila psittaci by Real-Time PCR and High-Resolution Melt Analysis

2008 ◽  
Vol 47 (1) ◽  
pp. 175-181 ◽  
Author(s):  
S. L. Mitchell ◽  
B. J. Wolff ◽  
W. L. Thacker ◽  
P. G. Ciembor ◽  
C. R. Gregory ◽  
...  
Keyword(s):  
High Resolution ◽  
Real Time ◽  
Real Time Pcr ◽  
High Resolution Melt ◽  
High Resolution Melt Analysis ◽  
Chlamydophila Psittaci

scholarly journals Development of a Novel Real-Time PCR Assay with High-Resolution Melt Analysis To Detect and Differentiate OXA-48-Like β-Lactamases in Carbapenem-Resistant Enterobacteriaceae

2015 ◽  
Vol 59 (9) ◽  
pp. 5574-5580 ◽  
Author(s):  
Peera Hemarajata ◽  
Shangxin Yang ◽  
Janet A. Hindler ◽  
Romney M. Humphries
Keyword(s):  
High Resolution ◽  
Real Time ◽  
Real Time Pcr ◽  
Hydrolytic Activity ◽  
The United States ◽  
Pcr Assay ◽  
High Resolution Melt ◽  
Content Type ◽  
High Resolution Melt Analysis ◽  
Carbapenem Resistant

ABSTRACTThe rapid global spread of carbapenem-resistantEnterobacteriaceae(CRE) poses an urgent threat to public health. More than 250 class D β-lactamases (OXAs) have been described in recent years, with variations in hydrolytic activity for β-lactams. The plasmid-borne OXA-48 β-lactamase and its variants are identified only sporadically in the United States but are common in Europe. Recognition of these OXA-48-like carbapenemases is vital in order to control their dissemination. We developed a real-time PCR assay based on high-resolution melt analysis, usingblaOXA-48-like-specific primers coupled with an unlabeled 3′-phosphorylated oligonucleotide probe (LunaProbe) homologous to OXA-48-like carbapenemase genes. The assay was validated using genomic DNA from 48 clinical isolates carrying a variety of carbapenemase genes, includingblaKPC,blaSME,blaIMP,blaNDM-1,blaVIM,blaOXA-48,blaOXA-162,blaOXA-181,blaOXA-204,blaOXA-244,blaOXA-245, andblaOXA-232. Our assay identified the presence ofblaOXA-48-likeβ-lactamase genes and clearly distinguished betweenblaOXA-48and its variants in control strains, including betweenblaOXA-181andblaOXA-232, which differ by only a single base pair in the assay target region. This approach has potential for use in epidemiological investigations and continuous surveillance to help control the spread of CRE strains producing OXA-48-like enzymes.

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