Identification and characterization of Serpulina hyodysenteriae by restriction enzyme analysis and Southern blot analysis.

ABSTRACT The question of whether the Ybb - chromosome contains ribosomal genes has been examined by using Southern blot analysis and comparing rDNA hybridization patterns for X/X and X/Ybb - DNA. The results demonstrate that the Ybb - chromosome contains sequences that hybridize to an rDNA probe under stringent conditions. Differential hybridization of some of these sequences with DNAs corresponding to different regions of a complete ribosomal gene repeat provides evidence that some of the genes on the Ybb - chromosome are type 2 repeats. Because data obtained by other workers suggest that type 2 repeats are transcribed only to low levels, these repeats may be classed as "nonfunctional". A further finding is that the ribosomal genes on the Ybb - chromosome do not undergo multiple rounds of DNA replication during polytenization of X/Ybb - cells.

Download Full-text

Characterization of neoplasia-associated chromosome abnormalities by Southern blot analysis

Clinical Genetics ◽

10.1111/j.1399-0004.1986.tb00528.x ◽

2008 ◽

Vol 29 (5) ◽

pp. 462-463

Author(s):

J. Kere ◽

T. Ruutu ◽

A. de la Chapelle

Keyword(s):

Southern Blot ◽

Blot Analysis ◽

Southern Blot Analysis ◽

Chromosome Abnormalities

Download Full-text

Characterization of fastidious adenovirus types 40 and 41 by DNA restriction enzyme analysis and by neutralizing monoclonal antibodies

Virus Research ◽

10.1016/0168-1702(89)90060-9 ◽

1989 ◽

Vol 12 (2) ◽

pp. 139-157 ◽

Cited By ~ 37

Author(s):

Harrie G.A.M. van der Avoort ◽

Anton G. Wermenbol ◽

Timo P.L. Zomerdijk ◽

John A.F.W. Kleijne ◽

Jack A.A.M. van Asten ◽

...

Keyword(s):

Monoclonal Antibodies ◽

Restriction Enzyme ◽

Enzyme Analysis ◽

Restriction Enzyme Analysis ◽

Dna Restriction

Download Full-text

Molecular Epidemiological Characterization of Respiratory Isolates ofMoraxella catarrhalisin a Pediatric Intensive Care Unit

Canadian Journal of Infectious Diseases ◽

10.1155/1992/370326 ◽

1992 ◽

Vol 3 (4) ◽

pp. 189-192 ◽

Cited By ~ 1

Author(s):

Anne G Matlow ◽

Donald E Low ◽

Gideon Paret ◽

Scott Jarrett ◽

Desmond Bohn ◽

...

Keyword(s):

Intensive Care Unit ◽

Intensive Care ◽

Pediatric Intensive Care Unit ◽

Restriction Enzyme ◽

Pediatric Intensive Care ◽

Respiratory Pathogen ◽

Enzyme Analysis ◽

Restriction Enzyme Analysis ◽

Strain Isolation

A perceived increase in the number of isolates ofMoraxella catarrhalisfrom the respiratory secretions of patients intubated in the pediatric intensive care unit prompted a review of the clinical profiles of such patients and restriction enzyme analysis of the strains involved. Over two months, of 192 patients admitted to the unit, 154 were intubated. Of the 46 for whom endotracheal tube specimens were submitted to the laboratory,M catarrhaliswas isolated in 12.M catarrhaliswas not felt to be a significant respiratory pathogen by the attending medical staff in any of the patients from whom it was isolated. In only two patients (17%) could nosocomial acquisition be firmly invoked. Restriction enzyme analysis of the 12 strains ruled out the presence of an epidemic strain. Isolation ofM catarrhalisfrom intubated children does not necessarily imply pathogenicity nor an outbreak situation.

Download Full-text

Comparison of the Genome of Ovine Adenovirus Types 1 Through 5 by Restriction Enzyme Analysis and DNA Hybridisation

Acta Veterinaria Hungarica ◽

10.1556/004.48.2000.4.10 ◽

2000 ◽

Vol 48 (4) ◽

pp. 477-484 ◽

Cited By ~ 4

Author(s):

Mária Benkő

Keyword(s):

Genome Size ◽

Southern Blot ◽

Restriction Enzyme ◽

New Genus ◽

Enzyme Analysis ◽

Restriction Enzyme Analysis ◽

Dna Hybridisation

The DNA of the prototype strains of ovine adenovirus (OAdV) 1 through 5 was analysed by restriction enzyme (RE) digestion. The RE patterns generated by Hindlll and PstI enzymes were characteristic of the examined strains. OAdV-2 and 3 resembled each other the most, and their EcoRI and Hindlll patterns seemed to be identical. Considering the number of comigrating fragments, serotypes OAdV-2, 3, 4 and 5 looked more closely related to each other than to OAdV-1. This finding was strengthened by Southern blot hybridisations probed with random Hindlll clones of OAdV-3. The estimated genome size of the examined OAdV types ranged between 31.9 and 32.8 kilobase pairs. The results supported the new genus classification of OAdVs.

Download Full-text

Identification and Characterization of a New Porin Gene of Klebsiella pneumoniae: Its Role in β-Lactam Antibiotic Resistance

Journal of Bacteriology ◽

10.1128/jb.181.9.2726-2732.1999 ◽

1999 ◽

Vol 181 (9) ◽

pp. 2726-2732 ◽

Cited By ~ 73

Author(s):

Antonio Doménech-Sánchez ◽

Santiago Hernández-Allés ◽

Luis Martínez-Martínez ◽

Vicente J. Benedí ◽

Sebastián Albertí

Keyword(s):

Escherichia Coli ◽

Klebsiella Pneumoniae ◽

Blot Analysis ◽

Southern Blot Analysis ◽

Functional Characterization ◽

Standard Laboratory ◽

Lactam Antibiotic ◽

Resistant Strains ◽

Identification And Characterization

ABSTRACT Klebsiella pneumoniae porin genes were analyzed to detect mutations accounting for the porin deficiency observed in many β-lactam-resistant strains. PCR and Southern blot analysis revealed the existence of a third porin gene in addition to the OmpK36 and OmpK35 porin genes previously described. This new porin gene was designated ompK37 and is present in all of the clinical isolates tested. The OmpK37 porin gene was cloned, sequenced, and overexpressed in Escherichia coli. In contrast to that of the major porins, OmpK37 porin expression was only detectable by Western blot analysis in porin-deficient β-lactam-resistant strains, suggesting strong down regulation under standard laboratory conditions. Functional characterization suggested a narrower pore for the OmpK37 porin than for K. pneumoniae porins OmpK36 and OmpK35. This correlated with the susceptibility to certain β-lactam antibiotics, since a K. pneumoniae strain expressing porin OmpK37, but not porin OmpK36 or OmpK35, was less susceptible to β-lactam antibiotics than the same strain expressing either porin OmpK36 or OmpK35.

Download Full-text