scholarly journals Evaluation of the Binax and Biotest Urinary Antigen Kits for Detection of Legionnaires' Disease Due to Multiple Serogroups and Species of Legionella

2000 ◽  
Vol 38 (7) ◽  
pp. 2763-2765 ◽  
Author(s):  
Robert F. Benson ◽  
Patrick W. Tang ◽  
Barry S. Fields
Keyword(s):  
Immunosorbent Assay ◽  
Broad Spectrum ◽  
Legionella Pneumophila ◽  
Urine Samples ◽  
Enzyme Linked Immunosorbent Assay ◽  
Urinary Antigen ◽  
Legionnaires Disease

The Binax and the Biotest urinary antigen kits for the detection of Legionnaires' disease caused by organisms other than Legionella pneumophila were compared by testing 45 urine samples from non-Legionella pneumophila serogroup 1 patients previously positive in a broad-spectrum enzyme-linked immunosorbent assay (ELISA). Eighteen were positive with the Binax kit, and 13 were positive with the Biotest. Although neither kit is as sensitive as ELISA, these results extend the number of serogroups and species ofLegionella that can be diagnosed with the Binax or Biotest kit.

scholarly journals Comparison of the Novel Immunocatch Legionella Test with Sofia Legionella FIA Assay and with BinaxNOW Legionella Card Assay for Detection ofLegionella pneumophila(Serogroup 1) Antigen in Urine Samples

2019 ◽  
Vol 57 (8) ◽  
Author(s):  
F. Congestrì ◽  
M. Morotti ◽  
R. Vicari ◽  
M. F. Pedna ◽  
M. Sparacino ◽  
...  
Keyword(s):  
Legionella Pneumophila ◽  
Signs And Symptoms ◽  
Laboratory Diagnosis ◽  
Antigen Detection ◽  
Urine Samples ◽  
Urinary Antigen ◽  
Content Type ◽  
High Clinical Suspicion ◽  
Legionnaires Disease ◽  
Urinary Antigen Detection

ABSTRACTLegionnaires’ disease (LD) refers to a serious form of acute pneumonia caused byLegionellaspecies. LD can be difficult to diagnose because the signs and symptoms are nonspecific, and therefore a rapid laboratory diagnosis is of paramount importance. In this study, a recently introduced immunochromatographic test (Immunocatch Legionella; Eiken Chemical Co., Ltd.) forLegionella pneumophila(serogroup 1) urinary antigen detection was compared with the Sofia Legionella fluorescent immunoassay (FIA) (Quidel) (routinely used in our laboratory) and with the widely used BinaxNOW Legionella assay (Alere). A total of 248 urine samples (60 frozen and 188 fresh) were evaluated. All of the samples were collected from patients with high clinical suspicion of Legionnaires’ disease. The three assays were performed simultaneously according to the manufacturers’ instructions. A total of 180 concordant negative and 66 concordant positive results were obtained. Only 2 discrepant results were registered. The sensitivity and specificity of Immunocatch compared with Sofia were, respectively, 98.5% and 99.4%. Cohen's kappa coefficient and overall percent agreement between Immunocatch and Sofia were also calculated and resulted in, respectively, 0.97 and 99.2%. These performances suggest that the Immunocatch test is a useful tool forLegionella pneumophila(serogroup 1) urinary antigen detection.

scholarly journals Evaluation of Vircell Enzyme-Linked Immunosorbent Assay and Indirect Immunofluorescence Assay for Detection of Antibodies against Legionella pneumophila

2006 ◽  
Vol 13 (3) ◽  
pp. 361-364 ◽  
Author(s):  
Bram M. W. Diederen ◽  
Jan A. J. W. Kluytmans ◽  
Marcel F. Peeters
Keyword(s):  
Sensitivity And Specificity ◽  
Immunosorbent Assay ◽  
Indirect Immunofluorescence ◽  
Legionella Pneumophila ◽  
Immunofluorescence Assay ◽  
Indirect Immunofluorescence Assay ◽  
Enzyme Linked Immunosorbent Assay ◽  
Clinical Sensitivity ◽  
Igm Elisa ◽  
Legionnaires Disease

ABSTRACT We evaluated the abilities of the Vircell immunoglobulin G (IgG) and IgM indirect immunofluorescence assay (IFA) for Legionella pneumophila serogroup 1, the IgM and IgG enzyme-linked immunosorbent assay (ELISA) for Legionella pneumophila serogroup 1, and the IgM-plus-IgG ELISA for Legionella pneumophila serogroups 1 to 6 to diagnose Legionnaires' disease (LD) in a well-described sample of patients with and without LD. Also, we determined the agreements, sensitivities, and specificities of the different Vircell assays in comparison to a validated ELISA (Serion classic ELISA). Clinical sensitivity and specificity were 74.6% and 96.6%, respectively, for the IgM IFA, 65.1% and 88.0% for the IgG IFA, 92.3% and 100% for the IgM ELISA, 43.3% and 96.6% for the IgG ELISA, and 90.8% and 100% for the IgM-plus-IgG ELISA. Compared to Serion classic ELISA, agreement, sensitivity, and specificity were 80.0%, 83.1%, and 78.4%, respectively, for the IgM IFA, 75.2%, 66.0%, and 79.5% for the IgG IFA, 89.5%, 82.0%, and 97.6% for the IgM ELISA, 81.9%, 88.9%, and 78.0% for the IgG ELISA, and 93.5%, 90.0%, and 96.6% for the IgM-plus-IgG ELISA. The value of a positive diagnostic result obtained by the Vircell IgM IFA, the Vircell IgG IFA, and the Vircell IgG ELISA might not be acceptable for a diagnostic assay. Both the high specificities and sensitivities of the Vircell IgM ELISA and the IgM-plus-IgG ELISA and the high correlation with the Serion classic ELISA indicate that they are useful in the diagnosis of LD.

Identification of the hemoglobin scavenger receptor/CD163 as a natural soluble protein in plasma

Blood ◽  
2002 ◽  
Vol 99 (1) ◽  
pp. 378-380 ◽  
Author(s):  
Holger Jon Møller ◽  
Niels Anker Peterslund ◽  
Jonas Heilskov Graversen ◽  
Søren Kragh Moestrup
Keyword(s):  
Electrophoretic Mobility ◽  
Immunosorbent Assay ◽  
Broad Spectrum ◽  
Interleukin 6 ◽  
Soluble Protein ◽  
Healthy Subjects ◽  
Scavenger Receptor ◽  
Enzyme Linked Immunosorbent Assay ◽  
Myelomonocytic Leukemia ◽  
Healthy Persons

The hemoglobin scavenger receptor (HbSR/CD163) is an interleukin-6– and glucocorticoid-regulated macrophage/monocyte receptor for uptake of haptoglobin-hemoglobin complexes. Moreover, there are strong indications that HbSR serves an anti-inflammatory function. Immunoprecipitation and immunoblotting enabled identification of a soluble plasma form of HbSR (sHbSR) having an electrophoretic mobility equal to that of recombinant HbSR consisting of the extracellular domain (scavenger receptor cysteine-rich 1-9). A sandwich enzyme-linked immunosorbent assay was established and used to measure the sHbSR level in 130 healthy subjects (median, 1.87 mg/L; range, 0.73-4.69 mg/L). To evaluate the sHbSR levels in conditions with increased leukocyte stimulation and proliferation, 140 patients admitted to a hematological department were screened. Several patients, with a broad spectrum of diagnoses, had a level of sHbSR above the range of healthy persons. Patients with myelomonocytic leukemias and pneumonia/sepsis exhibited the highest levels (up to 67.3 mg/L). In conclusion, sHbSR is an abundant plasma protein potentially valuable in monitoring patients with infections and myelomonocytic leukemia.

Simultaneous Analysis of T-2 Toxin and HT-2 Toxin by an Indirect Enzyme-Linked Immunosorbent Assay

1987 ◽  
Vol 70 (4) ◽  
pp. 657-661
Author(s):  
Titan S L Fan ◽  
Yi-Chun Xu ◽  
Fun Sun Chu
Keyword(s):  
Immunosorbent Assay ◽  
Indirect Elisa ◽  
Simultaneous Analysis ◽  
Urine Samples ◽  
Enzyme Linked Immunosorbent Assay ◽  
Toxin A ◽  
Toxin Concentration ◽  
Mixed Sample ◽  
Analytical Recovery ◽  
The Individual

Abstract An indirect enzyme-linked immunosorbent assay (ELISA) for the detection of HT-2 toxin in the presence or absence of T-2 toxin is described. In the indirect ELISA, the relative cross-reactivities of antibodies against T-2 toxin (anti-T-2) with T-2 toxin and HT-2 toxin were 1 and 0.1, whereas anti-HT-2 cross-reactivities with T-2 toxin and HT-2 toxin were 0.33 and 1, respectively. Using such relationships, a formula was established that could be used to calculate the individual toxin concentration in a mixed sample after experimentally analyzing for T-2 and HT-2 toxins in the 2 indirect ELISAs. This method was tested by analyzing urine samples spiked with HT-2 toxin alone and samples spiked with both T-2 toxin and HT-2 toxin. A cleanup protocol for treatment of urine samples before ELISA was also established. The overall analytical recovery of HT-2 toxin when it was added at concentrations of 0.1-10 parts per billion (ppb) to the urine samples was ca 89%. When both T-2 and HT-2 toxins were added to the urine samples at equal concentrations of 0.5 to 5.0 ppb, their recoveries were 112 and 109%, respectively.

scholarly journals Legionnaires' Disease - Results of a Multicenter Canadian Study

2003 ◽  
Vol 14 (3) ◽  
pp. 154-158 ◽  
Author(s):  
Thomas J Marrie ◽  
Emidio de Carolis ◽  
Victor L Yu ◽  
Janet Stout ◽  
Keyword(s):  
Legionella Pneumophila ◽  
Tertiary Care ◽  
Community Acquired Pneumonia ◽  
The Other ◽  
Urinary Antigen ◽  
Serum Samples ◽  
Eastern Canada ◽  
Legionnaires Disease ◽  
Canadian Provinces ◽  
A Prospective Study

BACKGROUND: There has never been a cross-Canada surveillance project to determine the rate ofLegionellaspecies as a cause of community-acquired pneumonia requiring hospitalization and to determine whether there are any regional differences in the rates of Legionnaires' disease in Canada. Anecdotally, Legionnaires' disease is thought to be uncommon in Western Canada.METHODS: From January, 1996 through to October 31, 1997, a prospective study of the etiology of community acquired pneumonia requiring admission to 15 tertiary care hospitals in eight Canadian provinces was conducted. A urine sample from each patient was tested forLegionella pneumophilaserogroup 1 antigen using a commercially available ELISA assay. A culture of sputum or other respiratory specimens for Legionellaceae was carried out at the discretion of the attending physician. Two hundred thirty-four patients had acute and 6-week convalescent serum samples tested for antibodies toL pneumophilaserogroups 1 through 6 using an ELISA method.RESULTS: 28 of the 850 patients (3.2%) had Legionnaires' disease; 18 of 823 (2.1%) were positive forL pneumophilaserogroup 1 by urinary antigen testing. The rate of Legionnaires' disease, based on urinary antigen, at the Halifax site was higher than that at the other sites (seven of 163 patients versus 11 of 660 [P=0.04]). Of the 28 cases of Legionnaires' disease identified using all methods, 11 of 277 patients (3.9%) were enrolled from Western provinces versus 17 of 573 patients (2.9%) from Eastern provinces (P=nonsignificant).CONCLUSIONS: Legionnaires' disease is just as common in Western as in Eastern Canada.L pneumophilaserogroup 1 may be more common in Halifax than at the other sites studied.

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