scholarly journals Potential Application of Nonstructural Protein NS1 Serotype-Specific Immunoglobulin G Enzyme-Linked Immunosorbent Assay in the Seroepidemiologic Study of Dengue Virus Infection: Correlation of Results with Those of the Plaque Reduction Neutralization Test

2002 ◽  
Vol 40 (5) ◽  
pp. 1840-1844 ◽  
Author(s):  
P.-Y. Shu ◽  
L.-K. Chen ◽  
S.-F. Chang ◽  
Y.-Y. Yueh ◽  
L. Chow ◽  
...  
Keyword(s):  
Virus Infection ◽  
Dengue Virus ◽  
Immunoglobulin G ◽  
Potential Application ◽  
Neutralization Test ◽  
Nonstructural Protein ◽  
Enzyme Linked Immunosorbent Assay ◽  
Dengue Virus Infection ◽  
Specific Immunoglobulin ◽  
Seroepidemiologic Study

scholarly journals Full Serotype- and Group-Specific NS1 Capture Enzyme-Linked Immunosorbent Assay for Rapid Differential Diagnosis of Dengue Virus Infection

2011 ◽  
Vol 18 (3) ◽  
pp. 430-434 ◽  
Author(s):  
Xixia Ding ◽  
Dongmei Hu ◽  
Yue Chen ◽  
Biao Di ◽  
Jing Jin ◽  
...  
Keyword(s):  
Differential Diagnosis ◽  
Virus Infection ◽  
Dengue Virus ◽  
Nonstructural Protein ◽  
Dengue Infection ◽  
Cross Reactivity ◽  
Enzyme Linked Immunosorbent Assay ◽  
Dengue Virus Infection ◽  
Effective Prevention ◽  
Nonstructural Protein 1

ABSTRACTDengue virus (DENV), a member of theFlavivirusfamily, has four distinct serotypes (DENV serotype 1 [DENV1], DENV2, DENV3, and DENV4) that require differentiation for the effective prevention of morbid disease. Early and rapid differentiation between flaviviruses remains challenging. Full assays combining four individual, serotype-specific and one group-specific nonstructural protein 1 (NS1) antigen capture enzyme-linked immunosorbent assays (ELISAs) based on monoclonal antibodies (MAbs) against DENV NS1 were developed and validated. The sensitivities and specificities of the full NS1 ELISAs were evaluated with viral cultures and dengue acute-phase sera. Four serotype-specific NS1 ELISAs displayed high specificities for the detection and differentiation of appropriate serotypes. The group-specific NS1 ELISA was broadly reactive with the four dengue virus serotypes. None of the NS1 ELISAs displayed cross-reactivity with the other flaviviruses or samples from febrile patients with non-dengue virus infections. The full serotype- and group-specific MAb-based NS1 capture ELISAs may provide tools for the early detection and typing of dengue infection, which is preferable to reverse transcriptase PCR (RT-PCR) for the rapid differential diagnosis of dengue virus infection in the field.

scholarly journals Immunoglobulin A Antibody Responses in Dengue Patients: a Useful Marker for Serodiagnosis of Dengue Virus Infection

2005 ◽  
Vol 12 (10) ◽  
pp. 1235-1237 ◽  
Author(s):  
M. Nawa ◽  
T. Takasaki ◽  
M. Ito ◽  
S. Inoue ◽  
K. Morita ◽  
...  
Keyword(s):  
Virus Infection ◽  
Dengue Virus ◽  
Immunoglobulin A ◽  
Antibody Responses ◽  
Enzyme Linked Immunosorbent Assay ◽  
Virus Infections ◽  
Serum Samples ◽  
Dengue Virus Infection ◽  
Iga Antibody ◽  
Antibody Capture

ABSTRACT We determined the usefulness of an immunoglobulin A (IgA) antibody-capture enzyme-linked immunosorbent assay for serodiagnosis of dengue virus infections. The results indicate that the presence of IgA and IgM in serum samples assures recent primary dengue virus infection even with a single serum sample.

scholarly journals Artificial Receptors in Serologic Tests for the Early Diagnosis of Dengue Virus Infection

2006 ◽  
Vol 52 (8) ◽  
pp. 1486-1491 ◽  
Author(s):  
Dar-Fu Tai ◽  
Chung-Yin Lin ◽  
Tzong-Zeng Wu ◽  
Jyh-Hsiung Huang ◽  
Pei-Yun Shu
Keyword(s):  
Virus Infection ◽  
Dengue Virus ◽  
Nonstructural Protein ◽  
Sample Pretreatment ◽  
Virus Infections ◽  
Serum Samples ◽  
Dengue Virus Infection ◽  
Artificial Receptors ◽  
Nonstructural Protein 1 ◽  
Sensitive Sensor

Abstract Background: Because of the range and nonspecificity of clinical presentations of dengue virus infections, we felt there was a need to create diagnostic tests. We used artificial receptors for the virus to develop serologic assays to detect dengue virus infection. Methods: We coated a quartz crystal microbalance (QCM) with molecularly imprinted polymers specific for nonstructural protein 1 of flavivirus. These artificial receptors were specifically created on a QCM chip by polymerization of monomers and were cross-linked in the presence of the epitope site of nonstructural protein 1. We tested serum samples from patients with confirmed cases of dengue reported to the Center for Disease Control in Taipei. Samples were diluted 100-fold; no other sample pretreatment was used. The QCM response was compared with results of monoclonal ELISA. Results: QCM signals were >15 Hz in 18 of 21 (86%) of dengue samples and in 0 of 16 control samples. The correlation (r2) of the QCM response and the ELISA result was 0.73. Within-run and run-to-run imprecisions (CV) were 4%–28% and 10%–32%, respectively. Conclusions: The described assay offers a serologic technique for diagnosis of early viremia. The results illustrate the potential of well-organized polymers on the highly sensitive sensor system for diagnostic and biotechnological applications.

scholarly journals Sero-diagnosis of Dengue virus in Different Hospitals of Nepal

2013 ◽  
Vol 1 (2) ◽  
pp. 58-62 ◽  
Author(s):  
Y Shah ◽  
G Khadka ◽  
GP Gupta ◽  
N Adhikari ◽  
A Poudel ◽  
...  
Keyword(s):  
Virus Infection ◽  
Dengue Virus ◽  
Dengue Fever ◽  
Control Measure ◽  
Public Health Problem ◽  
Enzyme Linked Immunosorbent Assay ◽  
Dengue Virus Infection ◽  
Capture Elisa ◽  
Occupation Group ◽  
Hilly Region

INTRODUCTION: Dengue fever (DF) is an emerging mosquito borne viral disease and important public health problem in low land Terai region which is also moving towards hilly region Nepal. This study was designed to determine the sero-prevalence of dengue virus infection in patients visiting hospitals of Nepal. MATERIALS AND METHODS: This study was conducted during period (June-November) of 2010 in Nepalese patients with fever visiting hospitals of Birganj, Damouli, Biratanagar, Dhading Besi and Chitwan. The sero-prevalence of dengue virus specific IgM was determined by enzyme linked immunosorbent assay (ELISA). Serum samples were collected from 289 patients visiting hospitals with history of fever and clinically suspected dengue fever. RESULTS: The anti-dengue IgM positivity was found to be 8.99%. The positive dengue cases were higher in male (10.8%) as compared to female (7.1%) though it was not statistically significant (P>0.05). Among different age groups, the highest positive cases (12.3%) were from age group below 15 years followed by above 50 years 8.3%. Out of 5 hospitals, the highest positive cases were in Tanahu hospital, Damouli (23.8%) followed by Bharatpur hospital and Chitwan (22.2%). Age and gender were found to be independent predictors. The highest numbers of dengue positive cases were in occupation group business (13.3%) followed by agriculture (12.7%). CONCLUSIONS: Prevalence of dengue virus infection is increasing and proper control measure should be provided. IgM capture ELISA was used for laboratory analysis and remains as a reliable and inexpensive method for the diagnosis of dengue. Hence, the IgM capture ELISA has become the most accepted technique for the diagnosis of dengue in developing countries like Nepal. DOI: http://dx.doi.org/10.3126/ijim.v1i2.7003 Int J Infect Microbiol 2012;1(1):58-62

scholarly journals Clinical Evaluation of a Rapid Immunochromatographic Test for the Diagnosis of Dengue Virus Infection

1998 ◽  
Vol 5 (3) ◽  
pp. 407-409 ◽  
Author(s):  
Chew Theng Sang ◽  
Lim Siew Hoon ◽  
Andrea Cuzzubbo ◽  
Peter Devine
Keyword(s):  
Virus Infection ◽  
Dengue Virus ◽  
Secondary Infection ◽  
Rapid Test ◽  
Immunoglobulin M ◽  
Immunochromatographic Test ◽  
Dengue Virus Infection ◽  
Hemagglutination Inhibition Assay ◽  
Specific Immunoglobulin ◽  
Paired Serum

ABSTRACT A rapid immunochromatographic test was compared to the hemagglutination inhibition assay for separate determinations of dengue virus-specific immunoglobulin M (IgM) and IgG levels in paired serum specimens from 92 patients (34 with primary dengue virus infection, 35 with secondary dengue virus infection, and 23 without dengue virus infection). The rapid test showed 99% sensitivity in the diagnosis of dengue virus infection. The majority (30 of 34 [88%]) of patients with primary infection showed positive IgM but negative IgG, while 34 of 35 (97%) patients with secondary infection showed positive IgG with or without IgM. Specificity in nonflavivirus infections was 96% (1 of 23 positive). The rapid test should be a useful aid in rapid diagnosis of dengue virus infection.

scholarly journals Incidence of Dengue Virus Infection in School-Aged Children in Puerto Rico: A Prospective Seroepidemiologic Study

2015 ◽  
Vol 92 (3) ◽  
pp. 486-491 ◽  
Author(s):  
D. Fermín Argüello ◽  
Kay M. Tomashek ◽  
Elizabeth Hunsperger ◽  
Luz Acosta ◽  
Christine Luxemburger ◽  
...  
Keyword(s):  
Puerto Rico ◽  
Virus Infection ◽  
Dengue Virus ◽  
Dengue Virus Infection ◽  
School Aged Children ◽  
Seroepidemiologic Study

scholarly journals Prospective Study To Determine Accuracy of Rapid Serological Assays for Diagnosis of Acute Dengue Virus Infection in Laos

2007 ◽  
Vol 14 (11) ◽  
pp. 1458-1464 ◽  
Author(s):  
Stuart D. Blacksell ◽  
David Bell ◽  
James Kelley ◽  
Mammen P. Mammen ◽  
Robert V. Gibbons ◽  
...  
Keyword(s):  
Virus Infection ◽  
Dengue Virus ◽  
Patient Management ◽  
Limited Resource ◽  
Immunoglobulin M ◽  
Enzyme Linked Immunosorbent Assay ◽  
Virus Infections ◽  
Serum Samples ◽  
Dengue Virus Infection ◽  
Resource Setting

ABSTRACT There is an urgent need for accurate and simple dengue virus infection diagnostic assays in limited-resource settings of dengue endemicity, to assist patient management. Using a panel of reference samples (S. D. Blacksell, P. N. Newton, D. Bell, J. Kelley, M. P. Mammen, D. W. Vaughn, V. Wuthiekanun, A. Sungkakum, A. Nisalak, and N. P. Day, Clin. Infect. Dis. 42:1127-1134, 2006), we recently evaluated eihgt commercially available immunochromatographic rapid diagnostic tests (RDTs) designed to detect dengue virus-specific immunoglobulin M (IgM) and/or IgG. We found that 6/8 RDTs had sensitivities of less than 50% (range, 6 to 65%), but specificities were generally high. Here, in conjuction with dengue virus serotyping by reverse transcriptase PCR and in the limited-resource setting of Laos, where dengue virus is endemic, we evaluated the same eight RDTs against a previously validated dengue IgM/IgG enzyme-linked immunosorbent assay for diagnosis of acute dengue virus infection. Paired serum samples were collected from 87 patients, of whom 38 had confirmed dengue virus infections (4 had primary infections, 33 had secondary infections, and 1 had an infection of indeterminate status). RDT sensitivity was low, with 7/8 RDTs having admission sample sensitivities of less than 20% (range, 4 to 26%). The majority (6/8) of the RDTs, demonstrated high specificity (>95%). Kappa statistic values ranged from 6 to 54% for the RDTs, demonstrating poor to moderate variation between three operators. No RDT adequately differentiated between primary and secondary dengue virus infections. The findings of this study suggest that currently available RDTs based on the detection of IgM antibodies for the diagnosis of acute dengue virus infections are unlikely to be useful for patient management.

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