scholarly journals Detection of Chlamydia trachomatis and Neisseria gonorrhoeae by Strand Displacement Amplification and Relevance of the Amplification Control for Use with Vaginal Swab Specimens

2003 ◽  
Vol 41 (8) ◽  
pp. 3592-3596 ◽  
Author(s):  
L. A. Cosentino ◽  
D. V. Landers ◽  
S. L. Hillier
Keyword(s):  
Chlamydia Trachomatis ◽  
Neisseria Gonorrhoeae ◽  
Vaginal Swab ◽  
Strand Displacement ◽  
Strand Displacement Amplification

The concordance of Chlamydia trachomatis genital infection between sexual partners, in the era of nucleic acid testing

Sexual Health ◽  
2005 ◽  
Vol 2 (1) ◽  
pp. 23 ◽  
Author(s):  
A. R. Markos
Keyword(s):  
Chlamydia Trachomatis ◽  
Sexual Partners ◽  
Contact Tracing ◽  
Genital Infection ◽  
Nucleic Acid Testing ◽  
Strand Displacement ◽  
Scientific Interest ◽  
Strand Displacement Amplification ◽  
Male Patients ◽  
Males And Females

Introduction: The rate of transmission of Chlamydia trachomatis, by infected males and females to their sexual partners, has been a matter of continued scientific interest and exploration. Methods: We examined the correlation of C. trachomatis infection in sexual partnerships, using Strand Displacement Amplification. Results: During July–November 2003, 97 male patients were reported positive for C. trachomatis. Fifty of the female sexual consorts were amenable for contact tracing, 38 of whom were identified as C. trachomatis positive. Within the same period, 93 female patients were C. trachomatis positive, and 56 male consorts were traceable, of whom 43 were positive for C. trachomatis. Conclusions: The concordance of C. trachomatis between sexual partners is in the region of 75%. This strengthens the case for epidemiological treatment for all consorts.

scholarly journals Chlamydia trachomatis, Neisseria gonorrhoeae, and Trichomonas vaginalis screening and treatment of pregnant women in Port-au-Prince, Haiti

2017 ◽  
Vol 28 (11) ◽  
pp. 1130-1134 ◽  
Author(s):  
Claire C Bristow ◽  
Patricia Mathelier ◽  
Oksana Ocheretina ◽  
Daphne Benoit ◽  
Jean W Pape ◽  
...  
Keyword(s):  
Chlamydia Trachomatis ◽  
Neisseria Gonorrhoeae ◽  
Pregnant Women ◽  
Laboratory Testing ◽  
Trichomonas Vaginalis ◽  
Opportunistic Infections ◽  
Newborn Health ◽  
Vaginal Swab ◽  
Sexually Transmitted ◽  
High Prevalence

In Haiti, routine screening for Chlamydia trachomatis (CT), Neisseria gonorrhoeae (NG), and Trichomonas vaginalis (TV) among pregnant women is not conducted; yet these sexually transmitted infections (STIs) are associated with adverse birth and newborn health outcomes. We aimed to assess the acceptability and feasibility of screening and the prevalence of STIs among pregnant women in Port-au-Prince, Haiti. Pregnant women of at least 18 years of age who attend Haitian Study Group for Kaposi’s sarcoma and Opportunistic Infections (GHESKIO) clinics in Port-au-Prince, Haiti provided self-collected vaginal swab specimens. Laboratory testing was done with Xpert® CT/NG and Xpert® TV. The results of this study showed that of the 322 pregnant women who visited GHESKIO for their regular scheduled appointments, 300 (93.2%) consented for CT, NG, and TV testing. Of those, 107 women (35.7%) tested positive for at least one STI. There were 42 (14.7%) cases of CT, 8 (2.8%) NG, and 83 (29.0%) TV infections. Most infections were treated – 122 of 133 (91.7%). In summary, we found that it was highly acceptable and feasible to implement CT, NG, and TV screening among pregnant women in Port-au-Prince, Haiti. We found high prevalence of STIs among pregnant women, which suggest that STI screening in this population may be warranted.

The significance of low-positive MOTA scoresin the BD-ProbeTec-Strand Displacement Amplification test for the detection of Neisseria gonorrhoeae

2007 ◽  
Vol 55 (3) ◽  
pp. e67-e68
Author(s):  
Thushan de Silva ◽  
Goura Kudesia ◽  
Anita Joall ◽  
Duncan Whittaker ◽  
Claire Ryan ◽  
...  
Keyword(s):  
Neisseria Gonorrhoeae ◽  
Strand Displacement ◽  
Strand Displacement Amplification

Performance Characteristics of the Becton Dickinson ProbeTec System for Direct Detection of Chlamydia trachomatis and Neisseria gonorrhoeae in Male and Female Urine Specimens in Comparison With the Roche Cobas System

2000 ◽  
Vol 124 (11) ◽  
pp. 1649-1652 ◽  
Author(s):  
Edward L. Chan ◽  
Ken Brandt ◽  
Karen Olienus ◽  
Nick Antonishyn ◽  
Greg B. Horsman
Keyword(s):  
Polymerase Chain Reaction ◽  
Chlamydia Trachomatis ◽  
Strand Displacement ◽  
Chain Reaction ◽  
Becton Dickinson ◽  
Strand Displacement Amplification ◽  
Male And Female ◽  
Female Urine ◽  
Polymerase Chain ◽  
Urine Specimens

Abstract Objective.—The Becton Dickinson BDProbeTec ET System is a new semiautomated system using strand displacement amplification technology that simultaneously amplifies and detects Chlamydia trachomatis and Neisseria gonorrhoeae DNA. The strand displacement amplification products are hybridized with a fluorescent detector probe and are captured by a chemiluminescent assay in a microwell format. An amplification control is also included to monitor assay inhibition. This study evaluated the performance of the BDProbTec ET system in detecting C trachomatis and N gonorrhoeae in male and female urine specimens, calculated its ability to process large volumes of specimens, and determined the inhibition rate. Materials and Methods.—Eight hundred twenty-five male and 399 female urine specimens were tested for both C trachomatis and N gonorrhoeae with the BDProbeTec ET system, and results were compared with those of the Roche Amplicor Cobas system. All urine specimens were processed on both assays on the same day they were received, according to the manufacturers' instructions. Discrepant results were resolved by in-house polymerase chain reaction assays. Internal or amplification controls were also used in each specimen assay to monitor inhibition. The throughput of the BDProbTec ET system was further tested with 150 urine specimens on an 8-hour shift for 2 days. Results.—The overall sensitivity, specificity, positive predicative value, and negative predicative value for for detection of chlamydia were 95.3%, 99.3%, 95.9%, and 99.2% for strand displacement amplification and 95.9%, 98.3%, 90.6%, and 99.3% for the Roche Amplicor system. For detection of gonorrhea, these values were 100%, 99.7%, 88.2%, and 100% and 96.7%, 98.9%, 69%, and 99.9%, respectively. The overall inhibition rates for both strand displacement amplification and Roche Amplicor were less than 3.5%. The BDProbTec ET system was able to produce 150 results each for chlamydia and gonorrhea and the internal control within the 8-hour shift. Conclusions.—The performance characteristics of the BDProbeTec ET assay are similar to those of the Roche Amplicor polymerase chain reaction for detection of chlamydia and gonorrhea in male and female urine specimens. The system was able to produce 300 results in an 8-hour shift.

Prevalence and risk factors associated with Chlamydia trachomatis, Neisseria gonorrhoeae, and Mycoplasma genitalium among women in Pelotas, Southern Brazil

2020 ◽  
Vol 31 (5) ◽  
pp. 432-439
Author(s):  
MF Silveira ◽  
MP Bruni ◽  
D Stauffert ◽  
D Golparian ◽  
M Unemo
Keyword(s):  
Risk Factors ◽  
Chlamydia Trachomatis ◽  
Neisseria Gonorrhoeae ◽  
Diagnostic Testing ◽  
Mycoplasma Genitalium ◽  
Vaginal Swab ◽  
Southern Brazil ◽  
Sexually Transmitted ◽  
Factors Associated ◽  
Gynecology And Obstetrics

The frequently asymptomatic sexually transmitted infections (STIs) caused by Chlamydia trachomatis (CT), Neisseria gonorrhoeae (NG), and Mycoplasma genitalium (MG) are poorly diagnosed in Brazil and can lead to severe complications/sequelae without timely detection and treatment. We investigated prevalence of CT, NG, and MG infections and associated demographic, behavioral, and clinical factors in consecutive women attending a gynecology and obstetrics outpatient clinic in Pelotas, Southern Brazil. Vaginal swab samples were prospectively obtained from asymptomatic and symptomatic women (n = 498) from August 2015 to December 2016 and tested with Aptima Combo2 and Aptima M. genitalium assays (Hologic). The prevalence of CT, NG, and MG was 6.8% (34/498), 1.0% (5/498), and 4.2% (21/498), respectively. Three (0.6%) cases of CT and NG co-infection and one (0.2%) case of CT and MG co-infection were identified. The risk factors associated with these bacterial STIs were youth (<30 years), no steady sexual partner, infection with additional STI, and lack of income. Bacterial STIs, particularly CT and MG, were prevalent among women, including pregnant women (60% of positive cases), in Pelotas, Brazil. Sensitive and specific diagnostic testing and early treatment are essential to control STIs, limit transmission chains, avoid future complications/sequelae, and reduce health and cost burdens on the population.

Sign in / Sign up

Export Citation Format

Share Document