scholarly journals Comparison of a New Lateral-Flow Chromatographic Membrane Immunoassay to Viral Culture for Rapid Detection and Differentiation of Influenza A and B Viruses in Respiratory Specimens

2004 ◽  
Vol 42 (8) ◽  
pp. 3661-3664 ◽  
Author(s):  
A. C. Cazacu ◽  
G. J. Demmler ◽  
M. A. Neuman ◽  
B. A. Forbes ◽  
S. Chung ◽  
...  
Keyword(s):  
Rapid Detection ◽  
Influenza A ◽  
Lateral Flow ◽  
Viral Culture ◽  
Respiratory Specimens

scholarly journals Clinical Evaluation of the New High-Throughput Luminex NxTAG Respiratory Pathogen Panel Assay for Multiplex Respiratory Pathogen Detection

2016 ◽  
Vol 54 (7) ◽  
pp. 1820-1825 ◽  
Author(s):  
Jonathan H. K. Chen ◽  
Ho-Yin Lam ◽  
Cyril C. Y. Yip ◽  
Sally C. Y. Wong ◽  
Jasper F. W. Chan ◽  
...  
Keyword(s):  
Real Time ◽  
Influenza A Virus ◽  
Real Time Pcr ◽  
Influenza A ◽  
Respiratory Pathogen ◽  
Gene Target ◽  
Viral Culture ◽  
Matrix Gene ◽  
Sample Throughput ◽  
Respiratory Specimens

A broad range of viral and bacterial pathogens can cause acute respiratory tract infection. For rapid detection of a broad respiratory pathogen spectrum, multiplex real-time PCR is ideal. This study evaluated the performance of the new Luminex NxTAG Respiratory Pathogen Panel (NxTAG-RPP) in comparison with the BioFire FilmArray Respiratory Panel (FA-RP) or singleplex real-time PCR as reference. A total of 284 clinical respiratory specimens and 3 influenza A/H7N9 viral culture samples were tested. All clinical specimens were processed and analyzed in parallel using NxTAG-RPP and the reference standard method. The H7N9 viral culture samples were tested using NxTAG-RPP only. Overall, the NxTAG-RPP demonstrated ≥93% sensitivity and specificity for all respiratory targets except human coronavirus OC43 (HCoV-OC43) and HCoV-HKU1. The H7N9 virus was detected by the influenza A virus matrix gene target, while other influenza A virus subtyping gene targets in the panel remained negative. Complete concordance between NxTAG-RPP and FA-RP was observed in 98.8% (318/322) of positive results (kappa = 0.92). Substantial agreement was found for most respiratory targets, but significant differences were observed in human metapneumovirus (P= 0.001) and parainfluenza virus type 3 (P= 0.031). NxTAG-RPP has a higher sample throughput than FA-RP (96 samples versus 1 sample per run) while the turnaround times for NxTAG-RPP and FA-RP were 5 h (up to 96 samples) and 1 h (for one sample), respectively. Overall, NxTAG-RPP demonstrated good diagnostic performance for most respiratory pathogens. The high sample throughput with reasonable turnaround time of this new assay makes it a suitable multiplex platform for routine screening of respiratory specimens in hospital-based laboratories.

scholarly journals Comparison of the performance of direct fluorescent antibody staining, a point-of-care rapid antigen test and virus isolation with that of RT-PCR for the detection of novel 2009 influenza A (H1N1) virus in respiratory specimens

2010 ◽  
Vol 59 (6) ◽  
pp. 713-717 ◽  
Author(s):  
Tina Ganzenmueller ◽  
Jeanette Kluba ◽  
Birgit Hilfrich ◽  
Wolfram Puppe ◽  
Willem Verhagen ◽  
...  
Keyword(s):  
Rapid Detection ◽  
Influenza A ◽  
Virus Isolation ◽  
H1n1 Virus ◽  
Point Of Care ◽  
Fluorescent Antibody ◽  
Rt Pcr ◽  
Influenza A H1n1 ◽  
Direct Fluorescent Antibody ◽  
Respiratory Specimens

Although infections with the novel pandemic 2009 influenza A (H1N1) virus (A/H1N1/2009) appeared to be relatively mild during the first summer of circulation (‘off season’), there has been significant morbidity and hospitalization and several fatal cases. Thus, rapid detection of A/H1N1/2009 is crucial for efficient treatment and infection control measures. In contrast to seasonal influenza, where point-of-care (POC) rapid antigen tests and direct fluorescent antibody (DFA) staining ensure rapid detection, diagnosis of A/H1N1/2009 has so far been based on RT-PCR. This study retrospectively compared the performance of the Quidel QuickVue POC test, DFA staining and virus isolation with that of RT-PCR for A/H1N1/2009 detection in 526 respiratory specimens collected during the first wave of the outbreak from May to September 2009. A/H1N1/2009 was detected in 9.1 % (48/526) of samples. One hundred and thirty-seven of the A/H1N1/2009 PCR-negative samples were additionally tested using a RealAccurate Respiratory RT-PCR panel, revealing other respiratory viruses (mainly entero/rhino- and adenoviruses) in 42.3 % (58/137). All methods analysed detected A/H1N1/2009 with excellent specificity but different sensitivities (POC test: 18.2 %; DFA staining: 38.7 %; virus isolation: 45.7 %). Therefore, the POC test was not suitable for diagnosis, detecting A/H1N1/2009 only if present in high concentrations (corresponding median C t value=19.0; range=16.5–21.4). DFA staining was also able to detect A/H1N1/2009 in specimens with a lower virus concentration (median C t value=24.0; range=16.5–29.8). Virus isolation, which was positive after a median time of 7.5 days, was too time-consuming. In summary, DFA staining is superior to POC testing and may be appropriate for patients expected to have a rather high level of virus replication. Nevertheless, in DFA-negative specimens, A/H1N1/2009 should be excluded by RT-PCR.

scholarly journals Lateral-flow immunoassay as a diagnostic test for influenza type A and B in children

2008 ◽  
Vol 48 (2) ◽  
pp. 104
Author(s):  
Ity Sulawati ◽  
Amalia Setyati ◽  
A. Samik Wahab ◽  
M. Juffrie
Keyword(s):  
Influenza Virus ◽  
Likelihood Ratio ◽  
Gold Standard ◽  
Influenza A ◽  
Positive Likelihood Ratio ◽  
Negative Likelihood Ratio ◽  
Type A ◽  
Lateral Flow ◽  
Lateral Flow Immunoassay ◽  
Viral Culture

Background The diagnosis of influenza remains difficult toestablish because of its similar symptoms to those of respiratoryinfection caused by other viruses. The “gold standard” for thediagnosis of influenza is viral culture, which takes time to gainthe result and is expensive as well. A simple, rapid, and easilyused tool for detection of influenza virus type A and B is needed.Objective To assess the accuracy of lateral-flow immunoassay withQuick Vue Influenza A+B ® in detecting influenza virus of typeA and B.Methods This was an observational study designed for diagnostictest. The subjects were children aged 0-14 years old presentingwith acute respiratory infection in primary Health Care Jetis ,Godean I, Godean II and Prof. Dr. Sardjito Hospital Yogyakarta,from October 2005 to May 2007. Specimens were collected fromboth the anterior nares and the throat by physicians for lateral-flow immunoassay with Quick Vue Influenza A+B ® and viralculture as gold standard. Lateral-flow immunoassay was done ineach study centre, nasal specimen was placed in an extractionreagent tube and sent to NAMRU II laboratory.Results There were 255 children enrolled in this study. Lateral-flow immunoassay by Quick Vue Influenza A+B ® has sensitivity70% (CI95% 6;83%), specificity 93% (CI95% 90;97%), positivepredictive value 68% (CI95% 54;82%), negative predictive value94% (CI95% 91;97%), positive likelihood ratio 10,56 (CI95%6,14;18,19) and negative likelihood ratio 0,32 (CI95% 0,21; 0,51).Conclusion Lateral-flow immunoassay (Quick Vue InfluenzaA+B ® ), nasal swab specimen is not accurate to detect influenzavirus A and B in children.

Evaluation of BioStar® FLU OIA® assay for rapid detection of influenza A and B viruses in respiratory specimens

2000 ◽  
Vol 17 (2) ◽  
pp. 119-126 ◽  
Author(s):  
M Hindiyeh ◽  
C Goulding ◽  
H Morgan ◽  
B Kenyon ◽  
J Langer ◽  
...  
Keyword(s):  
Rapid Detection ◽  
Influenza A ◽  
Respiratory Specimens

scholarly journals Infectivity of healthcare workers diagnosed with coronavirus disease 2019 (COVID-19) approximately 2 weeks after onset of symptoms: A cross-sectional study

2021 ◽  
pp. 1-3
Author(s):  
Yves Longtin ◽  
Hugues Charest ◽  
Caroline Quach ◽  
Patrice Savard ◽  
Mariana Baz ◽  
...  
Keyword(s):  
Symptom Onset ◽  
Healthcare Workers ◽  
Positive Culture ◽  
Cross Sectional Study ◽  
Sectional Study ◽  
Cross Sectional ◽  
Viral Shedding ◽  
Viral Culture ◽  
Respiratory Coronavirus ◽  
Respiratory Specimens

Abstract We performed viral culture of respiratory specimens in 118 severe acute respiratory coronavirus virus 2 (SARS-CoV-2)–infected healthcare workers (HCWs), ∼2 weeks after symptom onset. Only 1 HCW (0.8%) had a positive culture. No factors for prolonged viral shedding were identified. Infectivity is resolved in nearly all HCWs ∼2 weeks after symptom onset.

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