scholarly journals Expression of Bottom Component RNA of Cowpea Mosaic Virus in Cowpea Protoplasts

1980 ◽  
Vol 36 (2) ◽  
pp. 366-373 ◽  
Author(s):  
Geertje Rezelman ◽  
Rob Goldbach ◽  
Albert Van Kammen
Keyword(s):  
Mosaic Virus ◽  
Cowpea Mosaic Virus ◽  
Cowpea Protoplasts

scholarly journals Cowpea Mosaic Virus 32- and 60-Kilodalton Replication Proteins Target and Change the Morphology of Endoplasmic Reticulum Membranes

2002 ◽  
Vol 76 (12) ◽  
pp. 6293-6301 ◽  
Author(s):  
Jan E. Carette ◽  
Jan van Lent ◽  
Stuart A. MacFarlane ◽  
Joan Wellink ◽  
Ab van Kammen
Keyword(s):  
Endoplasmic Reticulum ◽  
Mosaic Virus ◽  
Close Association ◽  
Tobacco Rattle Virus ◽  
Viral Proteins ◽  
Cowpea Mosaic Virus ◽  
Replication Proteins ◽  
Replication Complex ◽  
Cytotoxic Proteins ◽  
Cowpea Protoplasts

ABSTRACT Cowpea mosaic virus (CPMV) replicates in close association with small membranous vesicles that are formed by rearrangements of intracellular membranes. To determine which of the viral proteins are responsible for the rearrangements of membranes and the attachment of the replication complex, we have expressed individual CPMV proteins encoded by RNA1 in cowpea protoplasts by transient expression and in Nicotiana benthamiana plants by using the tobacco rattle virus (TRV) expression vector. The 32-kDa protein (32K) and 60K, when expressed individually, accumulate in only low amounts but are found associated with membranes mainly derived from the endoplasmic reticulum (ER). 24K and 110K are freely soluble and accumulate to high levels. With the TRV vector, expression of 32K and 60K results in rearrangement of ER membranes. Besides, expression of 32K and 60K results in necrosis of the inoculated N. benthamiana leaves, suggesting that 32K and 60K are cytotoxic proteins. On the other hand, during CPMV infection 32K and 60K accumulate to high levels without causing necrosis.

Interactions of Cowpea Mosaic Virus with Cowpea Protoplasts)

1983 ◽  
Vol 107 (2) ◽  
pp. 182-191
Author(s):  
A. G. Smith ◽  
D. P. Durand ◽  
J. H. Hill
Keyword(s):  
Mosaic Virus ◽  
Cowpea Mosaic Virus ◽  
Cowpea Protoplasts

scholarly journals Expression of Cowpea Mosaic Virus M RNA in Cowpea Protoplasts

1989 ◽  
Vol 70 (11) ◽  
pp. 3043-3050 ◽  
Author(s):  
G. Rezelman ◽  
A. Van Kammen ◽  
J. Wellink
Keyword(s):  
Mosaic Virus ◽  
Cowpea Mosaic Virus ◽  
Cowpea Protoplasts

Is a helper factor necessary for infection of cowpea protoplasts with blackeye cowpea mosaic virus?

1987 ◽  
Vol 93 (1) ◽  
pp. 43-47 ◽  
Author(s):  
Jeanne Dijkstra ◽  
Nikolai A. M. Beek ◽  
Dick Lohuis ◽  
Maarten Helden ◽  
Roelof Meijer
Keyword(s):  
Mosaic Virus ◽  
Cowpea Mosaic Virus ◽  
Helper Factor ◽  
Blackeye Cowpea Mosaic Virus ◽  
Cowpea Protoplasts
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