scholarly journals Transactivation of a human cytomegalovirus early promoter by gene products from the immediate-early gene IE2 and augmentation by IE1: mutational analysis of the viral proteins.

1990 ◽  
Vol 64 (4) ◽  
pp. 1498-1506 ◽  
Author(s):  
C L Malone ◽  
D H Vesole ◽  
M F Stinski
Keyword(s):  
Human Cytomegalovirus ◽  
Mutational Analysis ◽  
Immediate Early Gene ◽  
Viral Proteins ◽  
Early Gene ◽  
Immediate Early ◽  
Gene Products ◽  
Early Promoter

scholarly journals The Human Cytomegalovirus UL35 Gene Encodes Two Proteins with Different Functions

2002 ◽  
Vol 76 (5) ◽  
pp. 2460-2468 ◽  
Author(s):  
Yingguang Liu ◽  
Bonita J. Biegalke
Keyword(s):  
Human Cytomegalovirus ◽  
Complex Structure ◽  
Protein A ◽  
Immediate Early Gene ◽  
Early Gene ◽  
Immediate Early ◽  
Terminal Portion ◽  
Virion Protein ◽  
Early Promoter ◽  
Major Immediate Early Promoter

ABSTRACT The human cytomegalovirus (HCMV) virion is a complex structure that contains at least 30 proteins, many of which have been identified. We determined that the HCMV UL35 gene encodes two proteins, including a previously unidentified virion protein. A 22-kDa phosphoprotein (ppUL35A) was translated from a 1.2-kb UL35 transcript by 4 h postinfection; a second phosphoprotein of 75 kDa (ppUL35) was translated from a 2.2-kb transcript predominantly late in infection. The 22-kDa protein localized to the nucleus, while the 75-kDa protein localized to the juxtanuclear compartment and was packaged into virion particles. The 22-kDa protein was identical to the COOH-terminal end of the 75-kDa protein but was not found in virions, thus defining the NH2-terminal portion of the 75-kDa protein as essential for packaging. Expression of the 22-kDa protein inhibited activation of the major immediate-early promoter by ppUL82 (pp71), suggesting that the UL35 22-kDa protein may modulate expression of the major immediate-early gene.

scholarly journals Requirements for Enhanced Transgene Expression by Untranslated Sequences from the Human Cytomegalovirus Immediate-Early Gene

1998 ◽  
Vol 4 (11) ◽  
pp. 700-706 ◽  
Author(s):  
Robert D. Simari ◽  
Zhi-Yong Yang ◽  
Xu Ling ◽  
Dominique Stephan ◽  
Neil D. Perkins ◽  
...  
Keyword(s):  
Human Cytomegalovirus ◽  
Transgene Expression ◽  
Immediate Early Gene ◽  
Early Gene ◽  
Immediate Early
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