Crystallin gene expression and lentoid body formation in quail embryo neuroretina cultures transformed by the oncogenic retrovirus Mill Hill 2 or Rous sarcoma virus

1986 ◽  
Vol 6 (11) ◽  
pp. 3704-3710
Author(s):  
L Simonneau ◽  
P Crisanti ◽  
A M Lorinet ◽  
F Alliot ◽  
Y Courtois ◽  
...  
Keyword(s):  
Rous Sarcoma Virus ◽  
Nonpermissive Temperature ◽  
Body Formation ◽  
Quail Embryo ◽  
Alpha Crystallin ◽  
Rous Sarcoma ◽  
Monolayer Cultures ◽  
Sarcoma Virus ◽  
Specific Proteins ◽  
Virus Mutant

The lens-specific proteins alpha and delta crystallins and lentoid bodies, structures that follow a differentiation pathway similar to that of the lens, regularly appear after 4 to 5 weeks in quail embryo neuroretina monolayer cultures. We have investigated the effects of the avian oncogenic retroviruses Mill Hill 2 and Rous sarcoma virus on this process. Quail embryo neuroretina cells transformed by Mill Hill 2 virus were established into permanent cultures that synthesized alpha and delta crystallins and contained stem cells for the production of lentoid bodies. In contrast, transformation with the Rous sarcoma virus mutant tsNY-68 blocked the appearance of mRNA crystallins, but cytoplasmic alpha and delta crystallin mRNA and alpha crystallin appeared 44 h after a shift to the nonpermissive temperature. However, delta crystallins and lentoid bodies were only present after 7 days. The crystallins of transformed quail neuroretina cultures were immunologically indistinguishable from those of quail lenses and of normal quail embryo neuroretina cultures.

scholarly journals Crystallin gene expression and lentoid body formation in quail embryo neuroretina cultures transformed by the oncogenic retrovirus Mill Hill 2 or Rous sarcoma virus.

1986 ◽  
Vol 6 (11) ◽  
pp. 3704-3710 ◽  
Author(s):  
L Simonneau ◽  
P Crisanti ◽  
A M Lorinet ◽  
F Alliot ◽  
Y Courtois ◽  
...  
Keyword(s):  
Rous Sarcoma Virus ◽  
Nonpermissive Temperature ◽  
Body Formation ◽  
Quail Embryo ◽  
Alpha Crystallin ◽  
Rous Sarcoma ◽  
Monolayer Cultures ◽  
Sarcoma Virus ◽  
Specific Proteins ◽  
Virus Mutant

The lens-specific proteins alpha and delta crystallins and lentoid bodies, structures that follow a differentiation pathway similar to that of the lens, regularly appear after 4 to 5 weeks in quail embryo neuroretina monolayer cultures. We have investigated the effects of the avian oncogenic retroviruses Mill Hill 2 and Rous sarcoma virus on this process. Quail embryo neuroretina cells transformed by Mill Hill 2 virus were established into permanent cultures that synthesized alpha and delta crystallins and contained stem cells for the production of lentoid bodies. In contrast, transformation with the Rous sarcoma virus mutant tsNY-68 blocked the appearance of mRNA crystallins, but cytoplasmic alpha and delta crystallin mRNA and alpha crystallin appeared 44 h after a shift to the nonpermissive temperature. However, delta crystallins and lentoid bodies were only present after 7 days. The crystallins of transformed quail neuroretina cultures were immunologically indistinguishable from those of quail lenses and of normal quail embryo neuroretina cultures.

A neuronal clone derived from a Rous sarcoma virus-transformed quail embryo neuroretina established culture

Nature ◽  
1983 ◽  
Vol 302 (5909) ◽  
pp. 616-618 ◽  
Author(s):  
Bernard Pessac ◽  
Arlette Girard ◽  
Georges Romey ◽  
Patricia Crisanti ◽  
Anne-Marie Lorinet ◽  
...  
Keyword(s):  
Rous Sarcoma Virus ◽  
Quail Embryo ◽  
Rous Sarcoma ◽  
Sarcoma Virus

scholarly journals Characterization of the genomic RNA from a Rous sarcoma virus mutant temperature sensitive for cell transformation

1979 ◽  
Vol 6 (2) ◽  
pp. 471-486 ◽  
Author(s):  
Jean-Luc Darlix ◽  
Mireille Levray ◽  
Peter A. Bromley ◽  
Pierre-François Spahr
Keyword(s):  
Rous Sarcoma Virus ◽  
Cell Transformation ◽  
Temperature Sensitive ◽  
Genomic Rna ◽  
Rous Sarcoma ◽  
Sarcoma Virus ◽  
Virus Mutant

scholarly journals Infection of chick limb bud presumptive chondroblasts by a temperature-sensitive mutant of Rous sarcoma virus and the reversible inhibition of their terminal differentiation in culture.

1983 ◽  
Vol 3 (8) ◽  
pp. 1518-1526 ◽  
Author(s):  
D Boettiger ◽  
R Soltesz ◽  
H Holtzer ◽  
M Pacifici
Keyword(s):  
Rous Sarcoma Virus ◽  
Limb Bud ◽  
Temperature Sensitive ◽  
Permissive Temperature ◽  
Nonpermissive Temperature ◽  
Sensitive Mutant ◽  
Temperature Sensitive Mutant ◽  
Rous Sarcoma ◽  
Sarcoma Virus ◽  
Infected Cells

Stage 21 to 22 chicken embryo limb bud cells were infected with a temperature-sensitive mutant of Rous sarcoma virus and were grown in culture. Although control, uninfected cells yielded definitive chondroblasts (by day 4) which initiated the synthesis of the cartilage-characteristic proteoglycan, the transformed cells grown at the permissive temperature failed to do so. These effects were fully reversible after a shift to the nonpermissive temperature. In addition, infected cells at the nonpermissive temperature expressed traits of terminal chondrogenic maturation 2 to 3 days earlier than parallel, uninfected cells. Thus, Rous sarcoma virus-induced transformation reversibly blocks terminal limb bud cell chondrogenesis in culture, at the nonpermissive temperature, viral infection may also induce intracellular or extracellular conditions which favor or accelerate the process of chondrogenic cell maturation.

scholarly journals Rous sarcoma virus mutant dlPA105 induces different transformed phenotypes in quail embryonic fibroblasts and neuroretina cells.

1987 ◽  
Vol 61 (8) ◽  
pp. 2530-2539 ◽  
Author(s):  
F Poirier ◽  
D Laugier ◽  
M Marx ◽  
G Dambrine ◽  
E A Garber ◽  
...  
Keyword(s):  
Rous Sarcoma Virus ◽  
Embryonic Fibroblasts ◽  
Rous Sarcoma ◽  
Sarcoma Virus ◽  
Virus Mutant

Infection of chick limb bud presumptive chondroblasts by a temperature-sensitive mutant of Rous sarcoma virus and the reversible inhibition of their terminal differentiation in culture

1983 ◽  
Vol 3 (8) ◽  
pp. 1518-1526
Author(s):  
D Boettiger ◽  
R Soltesz ◽  
H Holtzer ◽  
M Pacifici
Keyword(s):  
Rous Sarcoma Virus ◽  
Limb Bud ◽  
Temperature Sensitive ◽  
Permissive Temperature ◽  
Nonpermissive Temperature ◽  
Sensitive Mutant ◽  
Temperature Sensitive Mutant ◽  
Rous Sarcoma ◽  
Sarcoma Virus ◽  
Infected Cells

Stage 21 to 22 chicken embryo limb bud cells were infected with a temperature-sensitive mutant of Rous sarcoma virus and were grown in culture. Although control, uninfected cells yielded definitive chondroblasts (by day 4) which initiated the synthesis of the cartilage-characteristic proteoglycan, the transformed cells grown at the permissive temperature failed to do so. These effects were fully reversible after a shift to the nonpermissive temperature. In addition, infected cells at the nonpermissive temperature expressed traits of terminal chondrogenic maturation 2 to 3 days earlier than parallel, uninfected cells. Thus, Rous sarcoma virus-induced transformation reversibly blocks terminal limb bud cell chondrogenesis in culture, at the nonpermissive temperature, viral infection may also induce intracellular or extracellular conditions which favor or accelerate the process of chondrogenic cell maturation.

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