Extramitochondrial citrate synthase activity in bakers' yeast
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We isolated the gene for citrate synthase (citrate oxaloacetate lyase; EC 4.1.3.7) from Saccharomyces cerevisiae and ablated it by inserting the yeast LEU2 gene within its reading frame. This revealed a second, nonmitochondrial citrate synthase. Like the mitochondrial enzyme, this enzyme was sensitive to glucose repression. It did not react with antibodies against mitochondrial citrate synthase. Haploid cells lacking a gene for mitochondrial citrate synthase grew somewhat slower than wild-type yeast cells, but exhibited no auxotrophic growth requirements.
1986 ◽
Vol 6
(2)
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pp. 488-493
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1992 ◽
Vol 1111
(1)
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pp. 120-126
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1978 ◽
Vol 119
(2)
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pp. 213-235
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1993 ◽
Vol 13
(8)
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pp. 4826-4835
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1992 ◽
Vol 12
(9)
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pp. 4084-4092
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1991 ◽
Vol 11
(10)
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pp. 5101-5112
2001 ◽
Vol 21
(1)
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pp. 175-184
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