Antibodies to the ras gene product inhibit adenylate cyclase and accelerate progesterone-induced cell division in Xenopus laevis oocytes.
1986 ◽
Vol 6
(2)
◽
pp. 719-722
◽
Keyword(s):
Ras Gene
◽
Microinjection of monoclonal antibodies (lines 238, 172, and 259) directed against the ras gene product, p21, into Xenopus laevis oocytes accelerated progesterone-induced germinal vesicle breakdown. Antibody 238 had the greatest effect on the acceleration of progesterone-induced oocyte maturation, and this effect was correlated with in vitro inhibition of adenylate cyclase (EC 4.6.1.1) activity in a concentration-dependent manner. Inhibition of adenylate cyclase by antibody 238 was also measured in membranes prepared from oocytes pretreated with either cholera toxin or pertussis toxin. These results suggest a role for the ras gene product in the regulation of vertebrate cell adenylate cyclase activity.
1982 ◽
Vol 21
(1-3)
◽
pp. 127-132
◽
Keyword(s):
1974 ◽
Vol 88
(2)
◽
pp. 444-447
◽
1992 ◽
Vol 263
(4)
◽
pp. C896-C900
◽
1996 ◽
Vol 206
(2)
◽
pp. 110-124
◽