scholarly journals Genome Sequences of Eight Shiga Toxin-Producing Escherichia coli Strains Isolated from a Produce-Growing Region in California

2018 ◽  
Vol 7 (1) ◽  
Author(s):  
Craig T. Parker ◽  
Kerry K. Cooper ◽  
Steven Huynh ◽  
Timothy P. Smith ◽  
James L. Bono ◽  
...  

Produce contaminated with Shiga toxin-producing Escherichia coli (STEC) is a continuing source of foodborne illness in the United States. This report documents the complete genome sequences of eight STEC strains isolated from livestock and water samples taken from a major agricultural region for leafy greens in California.

2019 ◽  
Vol 8 (45) ◽  
Author(s):  
Michelle Q. Carter ◽  
Antares Pham ◽  
Diana K. Carychao ◽  
Michael B. Cooley

Escherichia coli strains RM9088 and RM10410 were isolated from crows near a leafy greens-growing region in California in April and July 2009, respectively. Both strains carry genes encoding Shiga toxins and other virulence factors in enteric pathogens. Here, we report the complete genome sequences of RM9088 and RM10410.


2013 ◽  
Vol 79 (13) ◽  
pp. 4164-4165 ◽  
Author(s):  
Musafiri Karama ◽  
Carlton L. Gyles

ABSTRACTShiga toxin-producingEscherichia coli(STEC) O111:NM is an important serotype that has been incriminated in disease outbreaks in the United States. This study characterized cattle STEC O111:NM for virulence factors and markers by PCR. Major conclusions are that STEC O111:NM characterized in this study lacksstx2and the full spectrum ofnlegene markers, and it has an incomplete OI-122.


2019 ◽  
Vol 8 (27) ◽  
Author(s):  
Beatriz Quiñones ◽  
Jaszemyn C. Yambao ◽  
Christopher J. Silva ◽  
Bertram G. Lee

ABSTRACT Shiga toxin-producing Escherichia coli O157:H7 is a foodborne pathogen and is responsible for outbreaks of human gastroenteritis. This report documents the draft genome sequences of nine O157:H7 cattle strains, which were identified to be PCR positive for a Shiga toxin gene but displayed different levels of functional toxin activity.


2019 ◽  
Vol 8 (9) ◽  
Author(s):  
Rebecca L. Lindsey ◽  
Lori A. Rowe ◽  
Dhwani Batra ◽  
Peyton Smith ◽  
Nancy A. Strockbine

Escherichia albertii is an emerging pathogen that is closely related to Escherichia coli and can carry some of the same virulence genes as E. coli. Here, we report the release of Illumina-corrected PacBio sequences for eight E. albertii genomes.


2017 ◽  
Vol 5 (44) ◽  
Author(s):  
Beatriz Quiñones ◽  
Jaszemyn C. Yambao ◽  
Bertram G. Lee

ABSTRACT Shiga toxin-producing Escherichia coli is a foodborne and waterborne pathogen and is responsible for outbreaks of human gastroenteritis. This report documents the draft genome sequences of seven O113:H21 strains recovered from livestock, wildlife, and soil samples recovered from a major agricultural region for leafy greens in California, USA.


2014 ◽  
Vol 53 (2) ◽  
pp. 579-586 ◽  
Author(s):  
Glen E. Mellor ◽  
Narelle Fegan ◽  
Kari S. Gobius ◽  
Helen V. Smith ◽  
Amy V. Jennison ◽  
...  

While the differential association ofEscherichia coliO157 genotypes with animal and human hosts has recently been well documented, little is known about their distribution between countries and how this might affect regional disease rates. Here, we used a 48-plex single nucleotide polymorphism (SNP) assay to segregate 148E. coliO157 isolates from Australia, Argentina, and the United States into 11 SNP lineages. We also investigated the relationship between SNP lineages, Shiga toxin (Stx) gene profiles, and total Stx production.E. coliO157 isolates clearly segregated into SNP lineages that were differentially associated with each country. Of the 11 SNP lineages, seven were detected among isolates from a single country, two were detected among isolates from all three countries, and another two were detected only among U.S. and Argentinean isolates. A number of Australian (30%) and Argentinean (14%) isolates were associated with novel, previously undescribed SNP lineages that were unique to each country. Isolates within SNP lineages that were strongly associated with the carriage ofstx2aproduced comparatively more Stx on average than did those lacking thestx2asubtype. Furthermore, the proportion of isolates instx2a-associated SNP lineages was significantly higher in Argentina and the United States than Australia (P< 0.05). This study provides evidence for the geographic divergence ofE. coliO157 and for a prominent role ofstx2ain total Stx production. These results also highlight the need for more comprehensive studies of the global distribution ofE. coliO157 lineages and the impacts of regionally predominantE. coliO157 lineages on the prevalence and severity of disease.


2018 ◽  
Vol 6 (19) ◽  
Author(s):  
Michelle Qiu Carter ◽  
Antares Pham

ABSTRACTEscherichia coliO145 strains RM14715 and RM14723 were isolated from wildlife feces near a leafy greens-growing region in Yuma, Arizona. Both strains carry a distinct genotype compared with theE. coliO145 strains isolated from Salinas Valley, California. Here we report complete genome sequences and annotations of RM14715 and RM14723.


2019 ◽  
Vol 8 (1) ◽  
Author(s):  
Craig T. Parker ◽  
Steven Huynh ◽  
James L. Bono ◽  
William G. Miller ◽  
Michael B. Cooley ◽  
...  

Non-O157 Shiga toxin-producing Escherichia coli (STEC) strains are a common source of foodborne illness. STEC O111 is among the most prevalent non-O157 STEC serogroups.


2015 ◽  
Vol 3 (6) ◽  
Author(s):  
Rebecca L. Lindsey ◽  
Kristen Knipe ◽  
Lori Rowe ◽  
Lisley Garcia-Toledo ◽  
Vladimir Loparev ◽  
...  

Shiga toxin-producing Escherichia coli (STEC) is an important foodborne pathogen. Here, we report complete whole-genome sequences for two STEC strains of serotypes O119:H4 and O165:H25 isolated from clinical cases in the United States.


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