scholarly journals In Vitro Evaluation of Antimicrobial Peptides from the Black Soldier Fly ( Hermetia Illucens ) against a Selection of Human Pathogens

2022 ◽  
Author(s):  
Laurence Van Moll ◽  
Jeroen De Smet ◽  
Anne Paas ◽  
Dorothee Tegtmeier ◽  
Andreas Vilcinskas ◽  
...  
Keyword(s):  
Antimicrobial Resistance ◽  
Drug Development ◽  
Antimicrobial Peptides ◽  
Human Pathogens ◽  
Resistance Development ◽  
Black Soldier Fly ◽  
In Vitro Evaluation ◽  
Conventional Antibiotics ◽  
Selection Of

With the ever growing antimicrobial resistance, finding new candidates for antimicrobial drug development is indispensable. Antimicrobial peptides have steadily gained attention as alternatives for conventional antibiotics, due to some highly desirable characteristics, such as their low propensity for resistance development.

scholarly journals Engineering of Marine-derived Antimicrobial Peptides (mAMPs) into Improved Anti-infective Drug Leads: A Mini-review

2021 ◽  
Vol 1192 (1) ◽  
pp. 012013
Author(s):  
L Sukmarini
Keyword(s):  
Antimicrobial Peptides ◽  
Chemical Diversity ◽  
Cytotoxic Activities ◽  
Small Molecular Weight ◽  
Resistance Development ◽  
Drug Candidates ◽  
Bacteria And Fungi ◽  
Drug Leads ◽  
Net Positive Charge

Abstract Marine-derived antimicrobial compounds possess chemical diversity varying from peptides, fatty acids to terpenes, alkaloids, and polyketides. These compounds, especially of peptide origin called antimicrobial peptides (AMPs), are present in the majority of marine organisms, including microbes (bacteria and fungi), invertebrates (molluscs, echinoderms, and sponges), vertebrates (fish and mammals), and plants (marine algae). They are defined by small molecular weight (less than 10 kDa), a net positive charge, and amphipathic structures. Moreover, due to their profound in vitro antimicrobial and cytotoxic activities and a low risk for resistance development, naturally occurring marine-derived AMPs (mAMPs) have been used as drug design templates for a large variety of semi-synthetic or synthetic AMPs, some of which have reached clinical trials. This mini-review aims to discuss AMPs from marine sources, mainly emphasizing the engineering of these peptides with improved pharmacological properties to develop drug candidates. Some selected recent examples of these engineered mAMPs as anti-infective drug leads are herein highlighted.

Antimicrobial peptides isolated from the blood of farm animals

2010 ◽  
Vol 50 (7) ◽  
pp. 660 ◽  
Author(s):  
Pak-Lam Yu ◽  
Danitsja S. van der Linden ◽  
Haryadi Sugiarto ◽  
Rachel C. Anderson
Keyword(s):  
Antimicrobial Peptides ◽  
Pathogenic Bacteria ◽  
Synergistic Effects ◽  
Waste Product ◽  
Pharmaceutical Products ◽  
Farm Animals ◽  
Antimicrobial Substances ◽  
Spectrum Activity ◽  
Conventional Antibiotics

The development of antimicrobial resistance by pathogenic bacteria has fuelled the search for alternatives to conventional antibiotics. Endogenous antimicrobial peptides have the potential to be used as new antimicrobial substances because they have low minimum inhibitory concentration in vitro, have broad-spectrum activity, neutralise lipopolysaccharides, promote wound healing and have synergistic effects with conventional antibiotics. Farm animals, in particular the blood that is a by-product of the meat and poultry industries, are an abundant, and currently underutilised, source of such antimicrobial peptides. These antimicrobial peptides could be isolated and developed into high-value products such as biopreservatives, topical neutraceutical products and pharmaceuticals. There have been some clinical trials of antimicrobial peptides as pharmaceutical products, but up to now, the trials have shown disappointing results. Further research and development is still needed before such peptides can be commercialised and full advantage taken of this waste product of the meat and poultry industries.

scholarly journals In vitro evaluation of the potential for resistance development to ceragenin CSA-13

2012 ◽  
Vol 67 (11) ◽  
pp. 2665-2672 ◽  
Author(s):  
J. E. Pollard ◽  
J. Snarr ◽  
V. Chaudhary ◽  
J. D. Jennings ◽  
H. Shaw ◽  
...  
Keyword(s):  
Resistance Development ◽  
In Vitro Evaluation

In vitro evaluation of antimicrobial resistance selection in Neisseria gonorrhoeae

2021 ◽  
pp. 106417
Author(s):  
George P. Allen ◽  
Kristina M. Deao ◽  
Stephanie A. Hill ◽  
Sandra M. Schipelliti ◽  
Thomas Tran
Keyword(s):  
Antimicrobial Resistance ◽  
Neisseria Gonorrhoeae ◽  
In Vitro Evaluation ◽  
Resistance Selection

scholarly journals Peptide Extract from Olivancillaria hiatula Exhibits Broad-Spectrum Antibacterial Activity

2018 ◽  
Vol 2018 ◽  
pp. 1-11 ◽  
Author(s):  
Edward Ntim Gasu ◽  
Hubert Senanu Ahor ◽  
Lawrence Sheringham Borquaye
Keyword(s):  
Antibacterial Activity ◽  
Antimicrobial Peptides ◽  
Broad Spectrum ◽  
Lag Phase ◽  
Marine Mollusc ◽  
Agar Diffusion ◽  
Global Concern ◽  
Dependent Growth ◽  
Conventional Antibiotics

Increasing reports of infectious diseases worldwide have become a global concern in recent times. Depleted antibiotic pipelines, rapid and complex cases of antimicrobial resistance, and emergence and re-emergence of infectious disease have necessitated an urgent need for the development of new antimicrobial therapeutics, preferably with novel modes of action. Due to their distinct mode of action, antimicrobial peptides offer an interesting alternative to conventional antibiotics to deal with the problems enumerated. In this study, the antimicrobial potential of the peptide extract from the marine mollusc, Olivancillaria hiatula, was evaluated in vitro. Agar diffusion and broth dilution techniques were used to evaluate microbial susceptibility to the peptide extract. Microplate-based assays were also used to investigate time-dependent growth inhibition profiles of microbes in the presence of peptide and evaluate the peptide’s ability to modulate the activities of standard antibiotics. Both Gram-positive and Gram-negative bacteria were inhibited by the peptide extract in the agar diffusion assay. The minimum inhibitory concentration (MIC) of peptide against test microorganisms was between 0.039 and 2.5 mg/mL. At the MIC, the peptide extract was bacteriostatic towards all tested microorganisms but bactericidal to Staphylococcus aureus. In the presence of the peptide extract, a prolonged lag phase was observed for all microbes, similar to standard ciprofloxacin. When administered together, peptide extracts enhanced the activities of ciprofloxacin and cefotaxime and were antagonistic towards erythromycin but indifferent towards metronidazole. Taken together, these results show the broad-spectrum antibacterial activity of peptide extract from Olivancillaria hiatula and demonstrate that antimicrobial peptides can be employed in combination with some conventional antibiotics for improved effects.

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