Recombinant small heat shock protein from Acholeplasma laidlawii increases the Escherichia coli viability in thermal stress by selective protein rescue

2017 ◽  
Vol 51 (1) ◽  
pp. 112-121 ◽  
Author(s):  
A. R. Kayumov ◽  
M. I. Bogachev ◽  
V. A. Manuvera ◽  
V. N. Lazarev ◽  
A. V. Sabantsev ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Thermal Stress ◽  
Heat Shock ◽  
Heat Shock Protein ◽  
Small Heat Shock Protein ◽  
Acholeplasma Laidlawii

Protective role of Mycobacterium leprae small heat-shock protein in heterologous hosts, Escherichia coli and Mycobacterium smegmatis, grown under stress

2013 ◽  
Vol 62 (7) ◽  
pp. 959-967 ◽  
Author(s):  
Jayapal Jeya Maheshwari ◽  
Kuppamuthu Dharmalingam
Keyword(s):  
Escherichia Coli ◽  
Heat Shock ◽  
Heat Shock Protein ◽  
Mycobacterium Smegmatis ◽  
Small Heat Shock Protein ◽  
Mycobacterium Leprae ◽  
Protective Role ◽  
Low Oxygen

The aim of this study is to examine the in vivo role of a small heat-shock protein (sHsp18) from Mycobacterium leprae in the survival of heterologous recombinant hosts carrying the gene encoding this protein under different environmental conditions that are normally encountered by M. leprae during its infection of the human host. Using an Escherichia coli system where shsp18 expression is controlled by its native promoter, we show that expression of shsp18 is induced under low oxygen tension, nutrient depletion and oxidative stress, all of which reflect the natural internal environment of the granulomas where the pathogen resides for long periods. We demonstrate the in vivo chaperone activity of sHsp18 through its ability to confer survival advantage to recombinant E. coli at heat-shock temperatures. Additional evidence for the protective role of sHsp18 was obtained when Mycobacterium smegmatis harbouring a copy of shsp18 was found to multiply better in human macrophages. Furthermore, the autokinase activity of sHsp18 protein demonstrated for what is believed to be the first time in this study implies that some of the functions of sHsp18 might be controlled by the phosphorylation state of this protein. Results from this study suggest that shsp18 might be one of the factors that facilitate the survival and persistence of M. leprae under stress and autophosphorylation of sHsp18 protein could be a mechanism used by this protein to sense changes in the external environment.

scholarly journals Regulation and Mechanism of Action of the Small Heat Shock Protein from the Hyperthermophilic ArchaeonPyrococcus furiosus

2001 ◽  
Vol 183 (17) ◽  
pp. 5198-5202 ◽  
Author(s):  
Pongpan Laksanalamai ◽  
Dennis L. Maeder ◽  
Frank T. Robb
Keyword(s):  
Escherichia Coli ◽  
Heat Shock ◽  
Heat Shock Protein ◽  
Mechanism Of Action ◽  
Pyrococcus Furiosus ◽  
Small Heat Shock Protein ◽  
Control Culture ◽  
Gene Encoding ◽  
E Coli

ABSTRACT The small heat shock protein (sHSP) from the hyperthermophilePyrococcus furiosus was specifically induced at the level of transcription by heat shock at 105°C. The gene encoding this protein was cloned and overexpressed in Escherichia coli. The recombinant sHSP prevented the majority of E. coli proteins from aggregating in vitro for up to 40 min at 105°C. The sHSP also prevented bovine glutamate dehydrogenase from aggregating at 56°C. Survivability of E. colioverexpressing the sHSP was enhanced approximately sixfold during exposure to 50°C for 2 h compared with the control culture, which did not express the sHSP. Apparently, the sHSP confers a survival advantage on mesophilic bacteria by preventing protein aggregation at supraoptimal temperatures.

The Temperature-Dependent Selectivity of Potential Interaction Partners for the Small Heat Shock Protein IbpA from Acholeplasma laidlawii

2016 ◽  
Vol 6 (4) ◽  
pp. 437-442 ◽  
Author(s):  
Innokentii E. Vishnyakov ◽  
Mikhail I. Bogachev ◽  
Ilnur Salafutdinov ◽  
Sergei N. Borchsenius ◽  
Airat R. Kayumov
Keyword(s):  
Heat Shock ◽  
Heat Shock Protein ◽  
Small Heat Shock Protein ◽  
Potential Interaction ◽  
Temperature Dependent ◽  
Acholeplasma Laidlawii ◽  
Interaction Partners

Overexpression of small heat shock protein 21 protects the Chinese oak silkworm Antheraea pernyi against thermal stress

2013 ◽  
Vol 59 (8) ◽  
pp. 848-854 ◽  
Author(s):  
Qiu-Ning Liu ◽  
Bao-Jian Zhu ◽  
Li-Shang Dai ◽  
Wei-Wei Fu ◽  
Kun-Zhang Lin ◽  
...  
Keyword(s):  
Thermal Stress ◽  
Heat Shock ◽  
Heat Shock Protein ◽  
Small Heat Shock Protein ◽  
Antheraea Pernyi

scholarly journals Heterologous Expression of a Plant Small Heat-Shock Protein Enhances Escherichia coli Viability under Heat and Cold Stress

1999 ◽  
Vol 120 (2) ◽  
pp. 521-528 ◽  
Author(s):  
Alvaro Soto ◽  
Isabel Allona ◽  
Carmen Collada ◽  
Maria-Angeles Guevara ◽  
Rosa Casado ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Heat Shock ◽  
Heat Shock Protein ◽  
Cold Stress ◽  
Heterologous Expression ◽  
Small Heat Shock Protein ◽  
Heat And Cold Stress
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