Phenotypic manifestation of gene expression encoding xyloglucanase from Penicillium canescens in transgenic aspen plants

2012 ◽  
Vol 59 (5) ◽  
pp. 618-625 ◽  
Author(s):  
K. A. Shestibratov ◽  
A. S. Podresov ◽  
M. A. Salmova ◽  
Yu. A. Kovalitskaya ◽  
E. O. Vidyagina ◽  
...  
Keyword(s):  
Gene Expression ◽  
Penicillium Canescens ◽  
Transgenic Aspen ◽  
Phenotypic Manifestation

scholarly journals Various effects of the expression of the xyloglucanase gene from Penicillium canescens in transgenic aspen under semi-natural conditions

2020 ◽  
Author(s):  
Elena O. Vidyagina ◽  
Natalia M. Subbotina ◽  
Vladimir A. Belyi ◽  
Vadim G. Lebedev ◽  
Konstantin V. Krutovsky ◽  
...  
Keyword(s):  
Cell Wall ◽  
Decomposition Rate ◽  
Transgenic Line ◽  
Carbohydrate Composition ◽  
Penicillium Canescens ◽  
Natural Conditions ◽  
Biochemical Traits ◽  
Transgenic Aspen ◽  
Transgenic Lines ◽  
Accelerated Growth

Abstract Background: Recombinant carbohydrases genes are used to produce transgenic woody plants with improved phenotypic traits. However, cultivation of such plants in open field is challenging due to by a number of problems. Therefore, additional research is needed to alleviate them. Results: Results of successful cultivation of the transgenic aspens ( Populus tremula ) carrying the recombinant xyloglucanase gene ( sp-Xeg ) from Penicillium canescens in semi-natural conditions are reported in this paper for the first time. Change of carbohydrate composition of wood was observed in transgenic aspens carrying the sp-Xeg gene. The transformed transgenic line Xeg-2-1b demonstrated accelerated growth and increased content of cellulose in wood of trees growing in both greenhouse and outside in comparison with the control untransformed line Pt. The accelerated growth was observed also in the transgenic line Xeg-1-1c. Thicker cell-wall and longer xylem fiber were also observed in both these transgenic lines. Undescribed earlier considerable reduction in the wood decomposition rate of the transgenic aspen stems was also revealed for the transformed transgenic lines. The decomposition rate was approximately twice as lower for the transgenic line Xeg-2-3b in comparison with the control untransformed line Pt. Conclusion: A direct dependence of the phenotypic and biochemical traits on the expression of the recombinant gene sp-Xeg was demonstrated. The higher was the level of the sp-Xeg gene expression, the more markedly were changes in the phenotypic and biochemical traits. All lines showed phenotypic changes in the leave traits. Our results showed that the plants carrying the recombinant sp-Xeg gene do not demonstrate a decrease in growth parameters in semi-natural conditions. In some transgenic lines, a change in the carbohydrate composition of the wood, an increase in the cell wall thickness and a decrease in the rate of decomposition of wood were observed.

Expression of xyloglucanase sp-Xeg gene from Penicillium canescens accelerates growth and rooting in transgenic aspen plants

2014 ◽  
Vol 50 (7) ◽  
pp. 701-708 ◽  
Author(s):  
E. O. Vidyagina ◽  
Yu. A. Kovalitskaya ◽  
D. S. Loginov ◽  
O. V. Koroleva ◽  
K. A. Shestibratov
Keyword(s):  
Penicillium Canescens ◽  
Transgenic Aspen

Protein inheritance and regulation of gene expression in yeast

2010 ◽  
Vol 8 (4) ◽  
pp. 10-16
Author(s):  
Ludmila N Mironova
Keyword(s):  
Gene Expression ◽  
Rapid Development ◽  
Good Reason ◽  
Regulation Of Gene Expression ◽  
Epigenetic Mechanism ◽  
Genetic Determinants ◽  
Phenotypic Manifestation ◽  
Cellular Population ◽  
Protein Nature

Prions of lower eukaryotes are genetic determinants of protein nature. Last years are marked by rapid development of the conception of prion inheritance. The list of yeast proteins, which have been shown to exist in the prion form in vivo, and phenotypic manifestation of prions provide good reason to believe that protein prionization may represent epigenetic mechanism regulating adaptability of a single cell and cellular population to environmental conditions.

scholarly journals Various effects of the expression of the xyloglucanase gene from Penicillium canescens in transgenic aspen under semi-natural conditions

2020 ◽  
Vol 20 (1) ◽  
Author(s):  
Elena O. Vidyagina ◽  
Natalia M. Subbotina ◽  
Vladimir A. Belyi ◽  
Vadim G. Lebedev ◽  
Konstantin V. Krutovsky ◽  
...  
Keyword(s):  
Cell Wall ◽  
Decomposition Rate ◽  
Transgenic Line ◽  
Carbohydrate Composition ◽  
Penicillium Canescens ◽  
Natural Conditions ◽  
Biochemical Traits ◽  
Transgenic Aspen ◽  
Transgenic Lines ◽  
Accelerated Growth

Abstract Background Recombinant carbohydrases genes are used to produce transgenic woody plants with improved phenotypic traits. However, cultivation of such plants in open field is challenging due to a number of problems. Therefore, additional research is needed to alleviate them. Results Results of successful cultivation of the transgenic aspens (Populus tremula) carrying the recombinant xyloglucanase gene (sp-Xeg) from Penicillium canescens in semi-natural conditions are reported in this paper for the first time. Change of carbohydrate composition of wood was observed in transgenic aspens carrying the sp-Xeg gene. The transformed transgenic line Xeg-2-1b demonstrated accelerated growth and increased content of cellulose in wood of trees growing in both greenhouse and outside in comparison with the control untransformed line Pt. The accelerated growth was observed also in the transgenic line Xeg-1-1c. Thicker cell-wall and longer xylem fiber were also observed in both these transgenic lines. Undescribed earlier considerable reduction in the wood decomposition rate of the transgenic aspen stems was also revealed for the transformed transgenic lines. The decomposition rate was approximately twice as lower for the transgenic line Xeg-2-3b in comparison with the control untransformed line Pt. Conclusion A direct dependence of the phenotypic and biochemical traits on the expression of the recombinant gene sp-Xeg was demonstrated. The higher was the level of the sp-Xeg gene expression, the more pronounced were changes in the phenotypic and biochemical traits. All lines showed phenotypic changes in the leave traits. Our results showed that the plants carrying the recombinant sp-Xeg gene do not demonstrate a decrease in growth parameters in semi-natural conditions. In some transgenic lines, a change in the carbohydrate composition of the wood, an increase in the cell wall thickness, and a decrease in the rate of decomposition of wood were observed.

scholarly journals Overexpression of the recombinant xyloglucanase <i>sp-Xeg</i> from <i>Penicillium canescens</i> accelerates growth and rooting of transgenic aspen plants

2013 ◽  
Vol 05 (06) ◽  
pp. 36-43
Author(s):  
Vidyagina Elena ◽  
Kovalitskaya Yulia ◽  
Loginov Dmitry ◽  
Koroleva Olga ◽  
Shestibratov Konstantin
Keyword(s):  
Penicillium Canescens ◽  
Transgenic Aspen

scholarly journals Various effects of the expression of the xyloglucanase gene from Penicillium canescens in transgenic aspen under semi-natural conditions

2020 ◽  
Author(s):  
Elena O. Vidyagina ◽  
Natalia M. Subbotina ◽  
Vladimir A. Belyi ◽  
Vadim G. Lebedev ◽  
Konstantin V. Krutovsky ◽  
...  
Keyword(s):  
Cell Wall ◽  
Decomposition Rate ◽  
Transgenic Line ◽  
Carbohydrate Composition ◽  
Penicillium Canescens ◽  
Natural Conditions ◽  
Biochemical Traits ◽  
Transgenic Aspen ◽  
Transgenic Lines ◽  
Accelerated Growth

Abstract Background: Recombinant carbohydrases genes are used to produce transgenic woody plants with improved phenotypic traits. However, cultivation of such plants in open field is challenging due to by a number of problems. Therefore, additional research is needed to alleviate them. Results: Results of successful cultivation of the transgenic aspens (Populus tremula) carrying the recombinant xyloglucanase gene (sp-Xeg) from Penicillium canescens in semi-natural conditions are reported in this paper for the first time. Change of carbohydrate composition of wood was observed in transgenic aspens carrying the sp-Xeg gene. The transformed transgenic line Xeg-2-1b demonstrated accelerated growth and increased content of cellulose in wood of trees growing in both greenhouse and outside in comparison with the control untransformed line Pt. The accelerated growth was observed also in the transgenic line Xeg-1-1c. Thicker cell-wall and longer xylem fiber were also observed in both these transgenic lines. Undescribed earlier considerable reduction in the wood decomposition rate of the transgenic aspen stems was also revealed for the transformed transgenic lines. The decomposition rate was approximately twice as lower for the transgenic line Xeg-2-3b in comparison with the control untransformed line Pt. Conclusion: A direct dependence of the phenotypic and biochemical traits on the expression of the recombinant gene sp-Xeg was demonstrated. The higher was the level of the sp-Xeg gene expression, the more markedly were changes in the phenotypic and biochemical traits. All lines showed phenotypic changes in the leave traits. Our results showed that the plants carrying the recombinant sp-Xeg gene do not demonstrate a decrease in growth parameters in semi-natural conditions. In some transgenic lines, a change in the carbohydrate composition of the wood, an increase in the cell wall thickness and a decrease in the rate of decomposition of wood were observed.

Molecular and Microscopic Analysis of Altered Gene Expression in Transgenic and Gene Targeted Mice

1996 ◽  
Vol 54 ◽  
pp. 12-13
Author(s):  
W. K. Jones ◽  
J. Robbins
Keyword(s):  
Gene Expression ◽  
Pulmonary Veins ◽  
Microscopic Analysis ◽  
Mouse Tissue ◽  
Adult Heart ◽  
Heavy Chains ◽  
Altered Gene Expression ◽  
Altered Gene ◽  
Pulmonary Myocardium

Two myosin heavy chains (MyHC) are expressed in the mammalian heart and are differentially regulated during development. In the mouse, the α-MyHC is expressed constitutively in the atrium. At birth, the β-MyHC is downregulated and replaced by the α-MyHC, which is the sole cardiac MyHC isoform in the adult heart. We have employed transgenic and gene-targeting methodologies to study the regulation of cardiac MyHC gene expression and the functional and developmental consequences of altered α-MyHC expression in the mouse.We previously characterized an α-MyHC promoter capable of driving tissue-specific and developmentally correct expression of a CAT (chloramphenicol acetyltransferase) marker in the mouse. Tissue surveys detected a small amount of CAT activity in the lung (Fig. 1a). The results of in situ hybridization analyses indicated that the pattern of CAT transcript in the adult heart (Fig. 1b, top panel) is the same as that of α-MyHC (Fig. 1b, lower panel). The α-MyHC gene is expressed in a layer of cardiac muscle (pulmonary myocardium) associated with the pulmonary veins (Fig. 1c). These studies extend our understanding of α-MyHC expression and delimit a third cardiac compartment.

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