Design of Domestic Physical Instruments for Rapid (without Alternation of Generations) Genotype Identification during Selection in Segregating and Wild Plant Populations

2020 ◽  
Vol 65 (10) ◽  
pp. 1558-1562
Author(s):  
V. A. Dragavtsev
Author(s):  
Robert C. Godfree ◽  
Lyndsey M. Vivian ◽  
Jennifer C. Pierson

2012 ◽  
Vol 7 (6) ◽  
pp. 602-604 ◽  
Author(s):  
Frances M. Shapter ◽  
Timothy L. Fitzgerald ◽  
Daniel L.E. Waters ◽  
Stuart McDonald ◽  
Ian H. Chivers ◽  
...  

2012 ◽  
Vol 99 (6) ◽  
pp. 1033-1042 ◽  
Author(s):  
H. R. Prendeville ◽  
X. Ye ◽  
T. Jack Morris ◽  
D. Pilson

2019 ◽  
Vol 94 (1) ◽  
Author(s):  
Yuxin Ma ◽  
Armelle Marais ◽  
Marie Lefebvre ◽  
Sébastien Theil ◽  
Laurence Svanella-Dumas ◽  
...  

ABSTRACT Metagenomic studies have indicated that the diversity of plant viruses was until recently far underestimated. As important components of ecosystems, there is a need to explore the diversity and richness of the viruses associated with plant populations and to understand the drivers shaping their diversity in space and time. Two viral sequence enrichment approaches, double-stranded RNA (dsRNA) and virion-associated nucleic acids (VANA), have been used and compared here for the description of the virome of complex plant pools representative of the most prevalent plant species in unmanaged and cultivated ecosystems. A novel bioinformatics strategy was used to assess viral richness not only at the family level but also by determining operational taxonomic units (OTU) following the clustering of conserved viral domains. A large viral diversity dominated by novel dsRNA viruses was detected in all sites, while a large between-site variability limited the ability to draw a clear conclusion on the impact of cultivation. A trend for a higher diversity of dsRNA viruses was nevertheless detected in unmanaged sites (118 versus 77 unique OTUs). The dsRNA-based approach consistently revealed a broader and more comprehensive diversity for RNA viruses than the VANA approach, whatever the assessment criterion. In addition, dissimilarity analyses indicated both approaches to be largely reproducible but not necessarily convergent. These findings illustrate features of phytoviromes in various ecosystems and a novel strategy for precise virus richness estimation. These results allow us to reason methodological choices in phytovirome studies and likely in other virome studies where RNA viruses are the focal taxa. IMPORTANCE There are today significant knowledge gaps on phytovirus populations and on the drivers impacting them but also on the comparative performance-methodological approaches for their study. We used and compared two viral sequence enrichment approaches, double-stranded RNAs (dsRNA) and virion-associated nucleic acids (VANA), for phytovirome description in complex pools representative of the most prevalent plant species in unmanaged and cultivated ecosystems. Viral richness was assessed by determining operational taxonomic units (OTU) following the clustering of conserved viral domains. There is some limited evidence of an impact of cultivation on viral populations. These results provide data allowing us to reason the methodological choices in virome studies. For researchers primarily interested in RNA viruses, the dsRNA approach is recommended because it consistently provided a more comprehensive description of the analyzed phytoviromes, but it understandably underrepresented DNA viruses and bacteriophages.


2017 ◽  
Vol 7 (6) ◽  
pp. 1762-1769 ◽  
Author(s):  
Tarald Seldal ◽  
Stein Joar Hegland ◽  
Knut Rydgren ◽  
Cesar Rodriguez-Saona ◽  
Joachim Paul Töpper

2013 ◽  
Vol 23 (4) ◽  
pp. 241-255 ◽  
Author(s):  
Alexa N. Weiss ◽  
Samantha B. Primer ◽  
Brian A. Pace ◽  
Kristin L. Mercer

AbstractGene flow between agricultural crops and related wild plant populations can produce hybrids which differ significantly from their wild counterparts in many life history traits, including seed traits. Seeds from wild annuals often possess significant dormancy, while cultivated varieties have been selected to germinate immediately under favourable conditions. Consequently, the dormancy characteristics of crop–wild hybrids could influence their survival, seed-bank dynamics and, ultimately, the extent to which crop genes persist in wild populations. Dormancy characteristics can be influenced by both maternal effects, as well as the genetics of a seed's embryo, which are the result of contributions from both parents. Here we focus on how maternal effects and embryo genetics affect seed dormancy in crop–wild hybrid sunflowers (Helianthus annuus). Using three laboratory experiments, we quantified the germination and dormancy of 15 crop–wild hybrid sunflower cross types, while also identifying achene characteristics that may influence the differential germination observed. We found that increased frequencies of crop alleles and the maternal effects imparted by hybridization can reduce dormancy, though the effect of increased frequencies of crop alleles was more pronounced in wild- and F1-produced than in the crop-produced achenes. The more open pericarp of the crop-produced achenes and the shorter relative distance that their radicles had to travel to germinate may explain some of the observed maternal effects. Finally, we generated hypotheses about how these results could affect survival and crop gene introgression in the field.


1992 ◽  
Vol 20 (5) ◽  
pp. 1153-1153 ◽  
Author(s):  
M. Loönn ◽  
H. Tegelstrom ◽  
H.C. Prentice

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