Effects of Th1 and Th2 cytokines on cytokine production and ICAM-1 expression on synovial fibroblasts.

e19028 Background: Melanomas produce soluble and cell-associated molecules that can suppress or alter antitumor immunity. Preclinical studies suggest the disease burden may alter the cytokine profile of helper T cell responses to cancer antigens. To describe the vaccine-induced production of Th2 or Th1 responses to helper peptides in the setting of advanced melanoma, we measured cytokine production of helper T cells responding to vaccination by 6 melanoma helper peptides (6MHP). We then analyzed results by measurable disease status. Methods: Thirty-nine patients with stage IIIB-IV melanoma received a 6MHP vaccine as a part of the UVA Mel41 clinical trial. For analysis of this correlative endpoint, antigen-reactive T cells were exposed to antigen, and supernatants (days 2 and 5) were assayed by cytokine bead arrays for Th1 and Th2 cytokines. Results: The dominant Th1 and Th2 cytokines detected were IFN-g and IL-5, respectively, though high levels of IL-2 were also produced early by sentinel lymph node lymphocytes. Th1 cytokine responses predominated both pre and post vaccine. Peripheral blood lymphocytes from patients with clinically measurable disease produced similar levels of total cytokine as patients with no evidence of disease (111pg/ml versus 174pg/ml respectively, p=0.44). For both disease statuses, the cytokine profile was Th1-dominant in most patients. Total cytokine production varied significantly among patients and varied over time but persisted up to nine months post-vaccine. Conclusions: The MHC class II-associated peptides used in this study induced helper T cells with a Th1-biased cytokine response in both PBMC and sentinel immunized nodes. However, both groups of patients can mount a Th1 dominant response to these peptides, which may persist long after the vaccination sequence. Future studies are needed to test newer vaccine adjuvants in combination with these peptides.

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Th1 and Th2 cytokines in early onset periodontitis

Immunology Letters ◽

10.1016/s0165-2478(97)88489-1 ◽

1997 ◽

Vol 56 (1-3) ◽

pp. 407

Author(s):

Z Opatrná

Keyword(s):

Early Onset ◽

Th2 Cytokines ◽

Th1 And Th2 Cytokines ◽

Th1 And Th2

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Effects of β-Lapachone on the Production of Th1 and Th2 Cytokines in C57BL/6 Mice

Journal of Environmental Pathology Toxicology and Oncology ◽

10.1615/jenvironpatholtoxicoloncol.v31.i2.10 ◽

2012 ◽

Vol 31 (2) ◽

pp. 87-94 ◽

Cited By ~ 1

Author(s):

Myungwon Choi ◽

Indal Park ◽

Jaehyun Ju ◽

Kun-Young Park ◽

Kwang Hyuk Kim

Keyword(s):

Th2 Cytokines ◽

Th1 And Th2 Cytokines ◽

Th1 And Th2

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The Th1 and Th2 cytokines IFN-γ and IL-4 antagonize the inhibition of monocyte IL-1 receptor antagonist by glucocorticoids: involvement of IL-1

European Journal of Immunology ◽

10.1002/(sici)1521-4141(199807)28:07<2075::aid-immu2075>3.0.co;2-0 ◽

1998 ◽

Vol 28 (7) ◽

pp. 2075-2085 ◽

Cited By ~ 14

Author(s):

Damián Kovalovsky ◽

Marcelo Páez Pereda ◽

Joachim Sauer ◽

Carolina Perez Castro ◽

Victor E. Nahmod ◽

...

Keyword(s):

Receptor Antagonist ◽

Th2 Cytokines ◽

Th1 And Th2 Cytokines ◽

Ifn Γ ◽

Th1 And Th2

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P. gingivalis-specific T-cell Lines Produce Th1 and Th2 Cytokines

Journal of Dental Research ◽

10.1177/154405910208100503 ◽

2002 ◽

Vol 81 (5) ◽

pp. 303-307 ◽

Cited By ~ 21

Author(s):

E. Gemmell ◽

C.L. Carter ◽

D.A. Grieco ◽

P.B. Sugerman ◽

G.J. Seymour

Keyword(s):

T Cell ◽

Cell Lines ◽

Th2 Cytokines ◽

Th1 And Th2 Cytokines ◽

T Cell Lines ◽

Th1 And Th2

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Modulation of Helper T Cytokines in Thymus during Early Pregnancy in Ewes

Animals ◽

10.3390/ani9050245 ◽

2019 ◽

Vol 9 (5) ◽

pp. 245 ◽

Cited By ~ 4

Author(s):

Leying Zhang ◽

Zimo Zhao ◽

Hao Mi ◽

Baoliang Liu ◽

Bin Wang ◽

...

Keyword(s):

Immune System ◽

Estrous Cycle ◽

Early Pregnancy ◽

Interleukin 2 ◽

Th2 Cytokines ◽

Maternal Immune System ◽

Th1 And Th2 Cytokines ◽

Ifn Γ ◽

Necrosis Factor ◽

Th1 And Th2

There is an immune tolerance in maternal immune system during pregnancy, and thymus is a main organ of the immune system. Helper T (Th)1 and Th2 cytokines are involved in the regulation of immune system, but the modulation of Th cytokines in the thymus during early pregnancy is unclear in ewes. Thymuses were collected on day 16 of the estrous cycle, and on days 13, 16, and 25 of pregnancy in ewes. qRT-PCR, Western blot, and immunohistochemistry were used to analyze the expression of Th1 and Th2 cytokines in the thymuses. There was a peak in the expression of interferon-gamma (IFN-γ) on day 16 of pregnancy, an upregulation of tumor necrosis factor beta (TNF-β), and a sustained expression of interleukin-2 (IL-2) and IL-4. Furthermore, there was a peak in the expression of IL-6 on day 13 of pregnancy, no expression of IL-6 on day 16 of the estrous cycle and day 25 of pregnancy, and an upregulation of IL-5 and IL-10 in the thymuses during early pregnancy. The immunohistochemistry results revealed that the IFN-γ and IL-6 proteins were limited to the stromal cells, capillaries, and thymic corpuscles. In conclusion, early pregnancy influenced the production of Th1 and Th2 cytokines of maternal thymus in sheep.

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