Calpain inhibitor I reduces colon injury caused by dinitrobenzene sulphonic acid in the rat

ABSTRACT Effects of inorganic ions on the uptake of chloromercuribenzene-p-sulphonic acid (CMBS) were studied in microdissected pancreatic islets of non-inbred ob/ob-mice. Na2SO4 stimulated the total islet cell uptake of CMBS but decreased the amount of CMBS remaining in islets after brief washing with L-cysteine. CaCl2 stimulated both the total and the cysteine-non-displaceable uptake; the stimulatory effect of CaCl2 on the cysteine-non-displaceable CMBS uptake was counteracted by Na2SO4. NaCl, KCl or choline chloride had no significant effect on the total islet cell uptake of CMBS, whereas LiCl was stimulatory. It is concluded that β-cells resemble erythrocytes in having a permeation path for CMBS that is inhibited by SO42−. By analogy with existing models of the erythrocyte membrane, it is suggested that the SO42−-sensitive path leads to sulphydryl groups controlling monovalent cationic permeability in β-cells.

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Faculty Opinions recommendation of The calpain inhibitor MDL28170 induces the expression of apoptotic markers in Leishmania amazonensis promastigotes.

Faculty Opinions – Post-Publication Peer Review of the Biomedical Literature ◽

10.3410/f.718359864.793494200 ◽

2014 ◽

Author(s):

Roberto Arenas ◽

Edoardo Torres-Guerrero

Keyword(s):

Leishmania Amazonensis ◽

Calpain Inhibitor ◽

Apoptotic Markers

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An Efficient Synthesis of 2-Substituted Quinazolin-4(3H)-ones by Using Recyclable Wang Resin Supported Sulfonic Acid Catalyst

Letters in Organic Chemistry ◽

10.2174/1570178617999201109203731 ◽

2020 ◽

Vol 17 ◽

Author(s):

Kalyani K. ◽

Srinivasa Reddy Kallam

Keyword(s):

Sulfonic Acid ◽

Functional Group ◽

Acid Catalyst ◽

Sulphonic Acid ◽

Isatoic Anhydride ◽

Efficient Synthesis ◽

Wang Resin ◽

Polymer Supported ◽

High Yields

Abstract:: An efficient synthesis of 2-substituted Quinazolin-4(3H)-ones has been developed from isatoic anhydride with various amidoximes by using recyclable polymer supported sulphonic acid catalyst. Excellent functional group compatibil-ity and high yields are the important features of this protocol.

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Determination of S-hydroxyquinoline-5-sulphonic acid

Collection of Czechoslovak Chemical Communications ◽

10.1135/cccc19581803 ◽

1958 ◽

Vol 23 (9) ◽

pp. 1803-1805

Author(s):

V. Velich

Keyword(s):

Sulphonic Acid

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Pulse polarographic study of the behaviour of some o,o'-dihydroxyazo-compounds

Collection of Czechoslovak Chemical Communications ◽

10.1135/cccc19891219 ◽

1989 ◽

Vol 54 (5) ◽

pp. 1219-1226 ◽

Cited By ~ 3

Author(s):

Enric Casassas ◽

Miquel Esteban ◽

Santiago Alier

Keyword(s):

Carboxylic Acid ◽

Reaction Mechanisms ◽

Sulphonic Acid ◽

Base Catalysis ◽

Polarographic Study ◽

Acid Base ◽

Eriochrome Black T ◽

Naphthoic Acid ◽

Calcon Carboxylic Acid

The reduction of several o,o'-dihydroxyazo-compounds is studied by means of pulse polarographic techniques (DPP, NPP and RPP). The compounds studied are the following: 2-(2'-hydroxyphenylazo)-phenol (o,o'-dihydroxyazobenzene), 1-(2'-hydroxy-1'-naphthylazo)-2-naphthol-4-sulphonic acid (calcon or Eriochrome Blue Black R), 1-(2'-hydroxy-4'-sulpho-1'-naphthylazo)-2-hydroxy-3-naphthoic acid (calcon carboxylic acid), and 1-(1'-hydroxy-2'-naphthylazo)-6-nitro-2-naphthol-4-sulphonic acid (Eriochrome Black T). Correlations between Ip and Epand experimental variables (pH, T, conc.) and instrumental parameters (dropping time, t, and pulse magnitude, ΔE) are established. Reaction mechanisms formerly proposed are discussed on the basis of the new obtained results, and the ranges are defined where adsorption and/or acid-base catalysis are operative.

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Calpain inhibitor-1 reduces renal ischemia/reperfusion injury in the rat

Kidney International ◽

10.1046/j.1523-1755.2001.0590062073.x ◽

2001 ◽

Vol 59 (6) ◽

pp. 2073 ◽

Cited By ~ 8

Author(s):

Prabal K. Chatterjee ◽

Paul A.J. Brown ◽

Salvatore Cuzzocrea ◽

Kai Zacharowski ◽

Keith N. Stewart ◽

...

Keyword(s):

Reperfusion Injury ◽

Ischemia Reperfusion Injury ◽

Ischemia Reperfusion ◽

Renal Ischemia ◽

Calpain Inhibitor ◽

Renal Ischemia Reperfusion Injury

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Calpain inhibitor A-558693 in experimental focal cerebral ischemia in rats

Neurological Research ◽

10.1179/016164105x15712 ◽

2005 ◽

Vol 27 (5) ◽

pp. 466-470 ◽

Cited By ~ 3

Author(s):

Martin Liebetrau ◽

Helge Martens ◽

Nora Thomassen ◽

Dorothe Burggraf ◽

Dusica Gabrijelcic-Geiger ◽

...

Keyword(s):

Cerebral Ischemia ◽

Focal Cerebral Ischemia ◽

Calpain Inhibitor

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Dental pulp stem cells overexpressing hepatocyte growth factor facilitate the repair of DSS-induced ulcerative colitis

Stem Cell Research & Therapy ◽

10.1186/s13287-020-02098-4 ◽

2021 ◽

Vol 12 (1) ◽

Author(s):

Ning Li ◽

Yichi Zhang ◽

Narayan Nepal ◽

Guoqing Li ◽

Ningning Yang ◽

...

Keyword(s):

Oxidative Stress ◽

Ulcerative Colitis ◽

Body Weight ◽

Stem Cell ◽

Dental Pulp ◽

The Other ◽

Therapeutic Effects ◽

Colon Injury ◽

Treatment Groups ◽

Hepatocyte Growth

Abstract Background Ulcerative colitis (UC) is a chronic and recurrent disease without satisfactory treatment strategies. Dental pulp stem cell (DPSC) transplantation has been proposed as a potential therapy for UC. This study aimed to investigate the therapeutic effects of the rat hepatocyte growth factor (HGF) gene transduced into DPSCs for UC. Methods The therapeutic effects of HGF-DPSCs transplanted intravenously into a rat model of UC induced by 5% dextran sulphate sodium (DSS) were compared with the other treatment groups (LV-HGF group, DPSCs group and GFP-DPSCs group). Immunofluorescence and immunohistochemistry were used to observe the localization and proliferation of HGF-DPSCs at the site of colon injury. The expression levels of inflammatory factors were detected by real-time quantitative PCR (RT-PCR) and western blotting. The oxidative stress markers were detected by ELISA. DAI scores and body weight changes were used to macroscopically evaluate the treatment of rats in each group. Results Immunofluorescence and immunohistochemistry assays showed that HGF-DPSCs homed to colon injury sites and colocalized with intestinal stem cell (ISC) markers (Bmi1, Musashi1 and Sox9) and significantly promoted protein expression (Bmi1, Musashi1, Sox9 and PCNA). Anti-inflammatory cytokine (TGF-β and IL-10) expression was the highest in the HGF-DPSCs group compared with the other treatment groups, while the expression of pro-inflammatory cytokines (TNF-α and INF-γ) was the lowest. Additionally, the oxidative stress response results showed that malondialdehyde (MDA) and myeloperoxidase (MPO) expression decreased while superoxide dismutase (SOD) expression increased, especially in the HGF-DPSCs group. The DAI scores showed a downward trend with time in the five treatment groups, whereas body weight increased, and the changes were most prominent in the HGF-DPSCs group. Conclusions The study indicated that HGF-DPSCs can alleviate injuries to the intestinal mucosa by transdifferentiating into ISC-like cells, promoting ISC-like cell proliferation, suppressing inflammatory responses and reducing oxidative stress damage, which provides new ideas for the clinical treatment of UC.

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Intracellular versus extracellular inhibition of calpain I causes differential effects on pain in a rat model of joint inflammation

Molecular Pain ◽

10.1177/17448069211016141 ◽

2021 ◽

Vol 17 ◽

pp. 174480692110161

Author(s):

Jason J McDougall ◽

Miranda McConnell ◽

Allison R Reid

Keyword(s):

Joint Pain ◽

Acute Inflammation ◽

Weight Bearing ◽

Joint Inflammation ◽

Local Administration ◽

Calpain Inhibitor ◽

Mast Cell Tryptase ◽

Calcium Dependent ◽

Protease Activated Receptor 2 ◽

Arthritic Joints

Calpain I is a calcium-dependent cysteine protease which has dual effects on tissue inflammation depending on its cellular location. Intracellularly, calpain I has pro-inflammatory properties but becomes anti-inflammatory when exteriorised into the extracellular space. In this study, the effect of calpain I on joint pain was investigated using the kaolin/carrageenan model of acute synovitis. Evoked pain behaviour was determined by von Frey hair algesiometry and non-evoked pain was measured using dynamic hindlimb weight bearing. Local administration of calpain I reduced secondary allodynia in the acute inflammation model and this effect was blocked by the cell impermeable calpain inhibitor E-64c. Calpain I also blocked the algesic effect of the protease activated receptor-2 (PAR-2) cleaving enzyme mast cell tryptase. The cell permeable calpain blocker E-64d also produced analgesia in arthritic joints. These data suggest that calpain I produces disparate effects on joint pain viz. analgesia when present extracellularly by disarming PAR-2, and pro-algesic when the enzyme is inside the cell.

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