scholarly journals S115 Pleural Fluid Adenosine Deaminase (ada) In The Diagnosis Of Tuberculous Pleural Effusions In A Low Incidence Population

Thorax ◽  
2014 ◽  
Vol 69 (Suppl 2) ◽  
pp. A62-A62 ◽  
Author(s):  
D. Arnold ◽  
R. Bhatnagar ◽  
L. Fairbanks ◽  
N. Zahan-Evans ◽  
A. Clive ◽  
...  
Keyword(s):  
Adenosine Deaminase ◽  
Pleural Fluid ◽  
Pleural Effusions ◽  
Low Incidence

Increased Pleural Fluid Adenosine Deaminase Levels in Patients with Malignant Pleural Effusions: A Potential Predictor of Talc Pleurodesis Outcome

Lung ◽  
2007 ◽  
Vol 185 (6) ◽  
pp. 349-354 ◽  
Author(s):  
Huseyin Yildirim ◽  
Muzaffer Metintas ◽  
Güntülü Ak ◽  
Sinan Erginel ◽  
Fusun Alatas ◽  
...  
Keyword(s):  
Adenosine Deaminase ◽  
Pleural Fluid ◽  
Pleural Effusions ◽  
Talc Pleurodesis ◽  
Potential Predictor ◽  
Malignant Pleural Effusions

DIAGNOSTIC SIGNIFICANCE OF PLEURAL FLUID INTERFERON GAMMA IN COMPARISON WITH ADENOSINE DEAMINASE IN TUBERCULOUS PLEURAL EFFUSIONS

CHEST Journal ◽  
2008 ◽  
Vol 134 (4) ◽  
pp. 54P
Author(s):  
Subhakar Kandi ◽  
Kishore Reddy Bhimireddy ◽  
Jaya Lakshmi Lingam ◽  
Prasad V. Surapaneni
Keyword(s):  
Adenosine Deaminase ◽  
Pleural Fluid ◽  
Interferon Gamma ◽  
Pleural Effusions ◽  
Diagnostic Significance

scholarly journals Pleural Fluid Adenosine Deaminase (Pfada) in the Diagnosis of Tuberculous Effusions in a Low Incidence Population

PLoS ONE ◽  
2015 ◽  
Vol 10 (2) ◽  
pp. e0113047 ◽  
Author(s):  
David T. Arnold ◽  
Rahul Bhatnagar ◽  
Lynette D. Fairbanks ◽  
Natalie Zahan-Evans ◽  
Amelia O. Clive ◽  
...  
Keyword(s):  
Adenosine Deaminase ◽  
Pleural Fluid ◽  
Low Incidence

scholarly journals Rapid automated determination of adenosine deaminase and lysozyme for differentiating tuberculous and nontuberculous pleural effusions

1996 ◽  
Vol 42 (2) ◽  
pp. 218-221 ◽  
Author(s):  
V Villena ◽  
J A Navarro-Gonzálvez ◽  
C García-Benayas ◽  
J A Manzanos ◽  
J Echave ◽  
...  
Keyword(s):  
Adenosine Deaminase ◽  
Pleural Fluid ◽  
Sensitivity And Specificity ◽  
Pleural Effusions ◽  
Pleural Biopsy ◽  
Cutoff Values ◽  
Serum Lysozyme ◽  
Automated Procedures ◽  
Automated Determination

Abstract The catalytic concentration of pleural adenosine deaminase (ADA) and the ratio of pleural lysozyme (PL) to serum lysozyme (SL) were measured in consecutive patients (49 tuberculous and 179 nontuberculous) with two automated procedures in a Hitachi 717 analyzer. Using sensitivity and specificity curves, we established cutoff values at 33 U/L for ADA and 1.7 for the PL/SL ratio. The sensitivity of ADA activities for tuberculous effusion was 90%, specificity 85%. Combining ADA with the PL/SL ratio enhanced specificity to 99%. However, high values for ADA and lysozyme ratios are not, alone or in combination, sensitive or specific enough to replace pleural biopsy or culture of pleural fluid for the diagnosis of tuberculous empyema.

scholarly journals Diagnostic Performance of Pleural Fluid Adenosine Deaminase for Tuberculous Pleural Effusion in a Low-Incidence Setting

2018 ◽  
Vol 56 (8) ◽  
Author(s):  
Matthew Blakiston ◽  
Weldon Chiu ◽  
Conroy Wong ◽  
Susan Morpeth ◽  
Susan Taylor
Keyword(s):  
Pleural Effusion ◽  
Adenosine Deaminase ◽  
Pleural Fluid ◽  
Diagnostic Performance ◽  
Area Under The Curve ◽  
Pleural Effusions ◽  
Roc Curve Analysis ◽  
Predictive Values ◽  
Content Type ◽  
Tuberculous Pleural Effusion

ABSTRACT The challenges associated with diagnosing tuberculous pleural effusion have led to the use of pleural fluid adenosine deaminase (pfADA) as a biomarker for Mycobacterium tuberculosis infection. This study retrospectively reviewed the diagnostic performance of pfADA, the pleural fluid lactate dehydrogenase (LD)/ADA ratio, and combinations of these two parameters in 1,637 episodes of pleural effusion in the low-tuberculosis (TB)-incidence setting of Auckland, Aotearoa New Zealand, from between March 2008 and November 2014. The median pfADA in 57 TB pleural effusion episodes (58.1 U/liter) was significantly higher (P < 0.001) than in 1,580 non-TB pleural effusions (11.4 U/liter). The median LD/ADA ratio in TB pleural effusion (8.2) was significantly lower (P < 0.001) than in non-TB pleural effusions (30.5). The pfADA and pleural fluid LD/ADA ratio AUCROC values (that is, receiver operating characteristic [ROC] curve analysis with determination of the ROC area under the curve) were 0.93 and 0.94, respectively. The pfADA thresholds of ≥15 and ≥30 U/liter demonstrated sensitivities of 100% (95% confidence internal = 93.7 to 100) and 93.0% (83.3 to 97.2), specificities of 62.7% (60.3 to 65.0) and 87.3% (85.6 to 88.9), positive predictive values (PPVs) of 8.8% (6.9 to 11.2) and 20.9% (16.4 to 26.4), and negative predictive values (NPVs) of 100% (99.6 to 100) and 99.7% (99.3 to 99.9), respectively. LD/ADA ratio thresholds of <25 and <15 demonstrated sensitivities of 100% (93.5 to 100) and 89.1% (78.2 to 94.9), specificities of 61.6% (59.1 to 64.0) and 84.8% (82.9 to 86.5), PPVs of 8.5% (6.6 to 10.9) and 17.3% (13.3 to 22.0), and NPVs of 100% (99.6 to 100) and 99.5% (99.0 to 99.8), respectively. A combination of pfADA ≥ 30 U/liter and an LD/ADA ratio < 15 increased the specificity and PPV to 97.8% (96.9 to 98.4) and 57.3% (46.5 to 67.5) but decreased the sensitivity to 85.5% (73.8 to 92.4). The primary value of pfADA in a low-TB-incidence setting, such as Auckland, is in utilization of its high NPV.

scholarly journals The pleural fluid lactate dehydrogenase/adenosine deaminase ratio differentiates between tuberculous and parapneumonic pleural effusions

2017 ◽  
Vol 17 (1) ◽  
Author(s):  
Jinlin Wang ◽  
Jun Liu ◽  
Xiaohong Xie ◽  
Panxiao Shen ◽  
Jianxing He ◽  
...  
Keyword(s):  
Lactate Dehydrogenase ◽  
Adenosine Deaminase ◽  
Pleural Fluid ◽  
Pleural Effusions

scholarly journals Diagnostic Accuracy of Adenosine deaminase enzyme (ADA) in the diagnosis of tuberculous pleural effusion

2020 ◽  
Vol 24 (4) ◽  
pp. 311-315
Author(s):  
Haroon Ur Rasheed ◽  
Ejaz Hassan Khan ◽  
Mohsin Shafi ◽  
Ahmad Rafiq ◽  
Ambreen Ali ◽  
...  
Keyword(s):  
Pleural Effusion ◽  
Diagnostic Accuracy ◽  
Adenosine Deaminase ◽  
Pleural Fluid ◽  
Predictive Value ◽  
Colorimetric Method ◽  
Pleural Effusions ◽  
Cross Sectional ◽  
Tuberculous Pleural Effusion ◽  
Systemic Examination

Objective: To study the diagnostic accuracy of Adenosine deaminase enzyme (ADA) in the diagnosis of tuberculous pleural effusion (TPE).Material and Methods: It was a cross-sectional descriptive study conducted in the Pulmonology departments of Lady Reading and Khyber Teaching Hospital Peshawar and department of Pathology, Khyber Medical College, Peshawar from April 2015 to Jan 2016. A total of 210 tuberculous and non-tuberculous pleural effusion patients were selected through consecutive non-probability sampling techniques. After physical and systemic examination, 3cc of pleural fluid was taken. ADA was estimated by Non-Guisti and Galanti method through the simple colorimetric method. All the data was entered in a specially designed proforma and SPSS v16 was used for statistical analysis.Results: Out of 210 tuberculous and non-tuberculous pleural effusions, the commonest cause of pleural effusion was tuberculosis followed by malignancy. In our study, Pleural fluid ADA levels have sensitivity, specificity, positive predictive value( PPV), and negative predictive value (NPV) of 95.5%, 92.3%, 92.4%, and 96% respectively in differentiating tuberculous pleural effusions from non-tuberculous lymphocytes predominant pleural effusions. Conclusion: Tuberculosis is the commonest infectious disease worldwide. A pleural fluid ADA level of ≥ 35 U/L in lymphocyte-predominant effusions makes mycobacterium tuberculosis most likely etiology. This test is not only very sensitive and specific but also it is very cheap, quick, and easy to perform by routine colorimetric method.  

scholarly journals Study of Diagnostic Importance of Adenosine Deaminase (ADA) Level in Pleural Effusions

2015 ◽  
Vol 2 (2) ◽  
pp. 104 ◽  
Author(s):  
Sachin Kate ◽  
B. K. Mutha ◽  
Gauri Kulkarni ◽  
Chetan Mahajan ◽  
Sushma Dugad
Keyword(s):  
Pleural Effusion ◽  
Adenosine Deaminase ◽  
Pleural Fluid ◽  
Sensitivity And Specificity ◽  
Predictive Value ◽  
Tertiary Care ◽  
High Sensitivity ◽  
Pleural Effusions ◽  
Tertiary Care Centre ◽  
Adenosine Deaminase Activity

<strong>Introduction</strong>: Pleural effusion is the abnormal accumulation of fluid in the pleural space. TB is the most common cause of pleural effusion worldwide (30-60%). The pleural fluid activity of adenosine deaminase (ADA) is one of the best, providing reliable basis for a treatment decision, particularly in excluding the diagnosis of tuberculosis, due to its high sensitivity.<strong> Aims and Objectives</strong>: To assess the importance of adenosine deaminase(ADA) level in the diagnosis of pleural effusion. To assess Adenosine Deaminase Activity (ADA) in tuberculosis pleural effusion and assess the sensitivity and specificity of ADA levels. <strong>Materials and Methods</strong>: This study was performed at the Department of Pulmonary Medicine at tertiary care centre. The study comprised of 75 patients of pleural effusion having Age &gt; 14 years, Clinical and Radiological evidence of Pleural Effusions&amp;Patients willing for ADA examination. Patients having Age &gt; 65 years, minimal nontappable effusion, not giving consent for ADA examination patient were excluded from the study. Detailed history, thorough physical examination, radiological findings, haematological and biochemical findings were recorded in the proforma. Pleural aspiration was performed on all patients. Macroscopic findings, cytological, microbiological and biochemical analysis of pleural fluid were performed in all patients including ADA level. PCR for Mycobacterium tuberculosis was also assessed in pleural fluid. Pleural fluid Adenosine deaminase level was measured by Giusti and Galanti method. <strong>Result</strong>: In our study out of 45 patients with tuberculosis pleural effusion ADA was more than 40IU/L in 42 (93.33%) and less than40IU/L in 3 (6.66 %). Our study showed a mean ADA of 107.7 IU/L Using a cut off of greater 40IU/L we got a sensitivity and specificity of 93.3% and 90% respectively and Positive predictive value 93.3% and Negative predictive value 90%. <strong>Conclusion</strong>: Pleural fluid ADA activity has been shown to be a valuable biochemical marker that has a high sensitivity and specificity for TB diagnosis.

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