Comparison of Three Anadromous Rainbow Trout (Oncorhynchus mykiss) Populations Using DNA Fingerprinting and Mixed DNA Samples

1994 ◽  
Vol 51 (S1) ◽  
pp. 252-257 ◽  
Author(s):  
Paul Spruell ◽  
Shawn A. Cummings ◽  
Yousok Kim ◽  
Gary H. Thorgaard
Keyword(s):  
Image Analysis ◽  
Genetic Variation ◽  
Rainbow Trout ◽  
Oncorhynchus Mykiss ◽  
Dna Fingerprinting ◽  
Steelhead Trout ◽  
Rainbow Trout Oncorhynchus Mykiss ◽  
Oligonucleotide Fingerprinting

DNA fingerprinting of 15 individuals from three populations of steelhead trout (Oncorhynchus mykiss) was compared with DNA fingerprinting of mixtures of 15 individuals from the same populations. Three oligonucleotide fingerprinting probes were used to detect genetic variation in individuals and mixtures. Scanning image analysis coupled with customized software was used to assign band identity. The degree of band sharing of individuals within and between populations was then determined. Although band-sharing estimates were similar within and between populations, bands found predominately in one population were identified. DNA fingerprinting of mixtures detected bands found only in the majority of the individuals in the mixture, allowing differences between populations to be more readily identified.

The influence of Wisconsinan glaciation and contemporary stream hydrology on microsatellite DNA variation in rainbow trout (Oncorhynchus mykiss)

2010 ◽  
Vol 67 (6) ◽  
pp. 919-935 ◽  
Author(s):  
P. Tamkee ◽  
E. Parkinson ◽  
E. B. Taylor
Keyword(s):  
Genetic Diversity ◽  
Genetic Variation ◽  
Rainbow Trout ◽  
Oncorhynchus Mykiss ◽  
Microsatellite Dna ◽  
Isolation By Distance ◽  
Habitat Characteristics ◽  
Dna Variation ◽  
Rainbow Trout Oncorhynchus Mykiss ◽  
Branching Points

Microsatellite DNA variation was examined in rainbow trout ( Oncorhynchus mykiss ) populations from throughout British Columbia, Canada, to address the roles of historical isolation, postglacial dispersal, and contemporary geomorphology in structuring genetic variation and differentiation. We detected signatures of historical isolation and postglacial recolonization in the form of “interior” and “coastal” population groupings, a decline in genetic variation as distance increased from putative glacial refugia, and different extents of isolation-by-distance in different regions. Rainbow trout populations were structured genetically into major regions and into smaller watersheds and then into drainages. Within drainages, high levels of dispersal and gene flow were inferred between geographically proximate and contiguous lakes. Elevation, stream branching points (nodes), fluvial distance, migration barriers, and stream and lake order influenced genetic diversity within, and differentiation among, populations. Habitat characteristics, particularly lake surface area and perimeter, were poor predictors of genetic variation. Although founder events and postglacial dispersal influenced broadscale patterns of genetic diversity in rainbow trout, our results suggest that contemporary factors can strongly modulate historical patterns.

scholarly journals Multilocus DNA-fingerprinting using oligonucleotide probes (GATA)4 and (GGAT)4 in rainbow trout (Oncorhynchus mykiss)

1998 ◽  
Vol 21 (2) ◽  
pp. 241-243
Author(s):  
N. Vergara ◽  
P. Iturra ◽  
Aguirre
Keyword(s):  
Rainbow Trout ◽  
Oncorhynchus Mykiss ◽  
Dna Fingerprinting ◽  
Oligonucleotide Probes ◽  
Rainbow Trout Oncorhynchus Mykiss ◽  
Multilocus Dna Fingerprinting ◽  
Gynogenetic Offspring

Oligonucleotide probes (GATA)4 and (GGAT)4 for minisatellite loci produced DNA fingerprinting patterns which are unique in individual rainbow trout. These patterns can be used to monitor the efficiency of manipulation of production characters in gynogenetic individuals. The (GATA)4 probe produced highly individual patterns that differentiated individuals among gynogenetic offspring, and could be easily scored.

Hybridization with Domesticated Rainbow Trout (Oncorhynchus mykiss) Reduces Seasonal Variation in Growth of Steelhead Trout (O. mykiss)

1993 ◽  
Vol 50 (3) ◽  
pp. 480-487 ◽  
Author(s):  
Jörgen I. Johnsson ◽  
W. Craig Clarke ◽  
Ruth E. Withler
Keyword(s):  
Growth Rate ◽  
Seasonal Variation ◽  
Rainbow Trout ◽  
Oncorhynchus Mykiss ◽  
Natural Waters ◽  
Salt Water ◽  
Feeding Activity ◽  
Steelhead Trout ◽  
Rainbow Trout Oncorhynchus Mykiss ◽  
Winter Conditions

We tested the hypothesis that hybridization with domesticated rainbow trout (Oncorhynchus mykiss) reduces the seasonal variation in growth in both fresh and salt water in anadromous steelhead trout (O. mykiss). The seasonal variation in freshwater growth was more pronounced in the steelhead trout than in the hybrid and domesticated trout. Consequently, differences in growth rate among groups were considerably higher during winter than in fall or spring. We suggest that high feeding activity during unfavourable winter conditions in natural waters is maladaptive, but is not selected against in protected hatchery environments with a continuous food supply. This may lead to genetic divergence in feeding physiology and/or behaviour between steelhead trout and domesticated rainbow trout. Growth rates in experimental groups transferred to seawater in December relative to groups transferred in April were faster in rainbow and hybrid trout than in steelhead trout. The results support the hypothesis that seasonal variation in seawater growth in steelhead trout is reduced by crossing with domesticated rainbow trout.

Genetic variation in lysozyme activity in rainbow trout (Oncorhynchus mykiss)

Aquaculture ◽  
1993 ◽  
Vol 109 (3-4) ◽  
pp. 237-244 ◽  
Author(s):  
Knut H. Røed ◽  
Hans Jørgen S. Larsen ◽  
Ralph D. Linder ◽  
Terje Refstie
Keyword(s):  
Genetic Variation ◽  
Rainbow Trout ◽  
Oncorhynchus Mykiss ◽  
Lysozyme Activity ◽  
Rainbow Trout Oncorhynchus Mykiss
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