Polymorphic regions in plant genomes detected by an M13 probe

Genome ◽  
1989 ◽  
Vol 32 (5) ◽  
pp. 824-828 ◽  
Author(s):  
P. A. Zimmerman ◽  
N. Lang-Unnasch ◽  
C. A. Cullis
Keyword(s):  
Animal Species ◽  
Fragment Length Polymorphism ◽  
Higher Plants ◽  
Genome Chromosome ◽  
Plant Genomes ◽  
Bacteriophage M13 ◽  
Hypervariable Minisatellites ◽  
M13 Probe ◽  
Restriction Fragment Length ◽  
Dicot Species

A sequence containing two clusters of 15 base pair repeats from the protein III gene in the bacteriophage M13 has been shown to detect hypervariable minisatellites in many animal species. We have shown that this M13 fragment will detect similar sequences in higher plants including monocots, dicots, and gymnosperms. In addition, polymorphisms were demonstrated in three dicot species. The amount of this sequence has no relationship with the size of the genome, chromosome number, or amount of repetitive DNA in the species tested.Key words: bacteriophage M13, monocot, dicot, gymnosperm, restriction fragment length polymorphism.

Variation in the Promoter Region of the β Fibrinogen Gene Is Associated with Plasma Fibrinogen Levels in Smokers and Non-Smokers

1991 ◽  
Vol 65 (05) ◽  
pp. 487-490 ◽  
Author(s):  
A E Thomas ◽  
F R Green ◽  
C H Kelleher ◽  
H C Wilkes ◽  
P J Brennan ◽  
...  
Keyword(s):  
Heart Disease ◽  
Promoter Region ◽  
Fragment Length Polymorphism ◽  
Fibrinogen Level ◽  
Plasma Fibrinogen ◽  
Plasma Fibrinogen Level ◽  
Healthy Men ◽  
History Of ◽  
General Practices ◽  
Restriction Fragment Length

SummaryWe investigated the association between fibrinogen levels and a HaeIII restriction fragment length polymorphism located at −453 bp from the start of transcription of the β fibrinogen gene. 292 healthy men aged 45 to 69 years, recruited from general practices throughout Britain, were studied. None had a history of ischaemic heart disease. 41.1% (120) were smokers and fibrinogen levels were higher in this group. The frequency of the noncutting allele (designated H2) was 0.19 and was the same in smokers and non-smokers. The H2 allele was associated with elevated levels of fibrinogen in both smokers and non-smokers and the effect of genotype was similar in both groups. After smoking, HaeIII genotype was the strongest predictor of fibrinogen levels and explained 3.1% of the variance in fibrinogen levels. These results confirm earlier studies that variation at the fibrinogen locus contributes to the between-individual differences in plasma fibrinogen level.

Restriction Fragment Length Polymorphism in Soybean Germplasm of the Southern USA

Crop Science ◽  
1993 ◽  
Vol 33 (6) ◽  
pp. 1169-1176 ◽  
Author(s):  
H. T. Skorupska ◽  
R. C. Shoemaker ◽  
A. Warner ◽  
E. R. Shipe ◽  
W. C. Bridges
Keyword(s):  
Restriction Fragment Length Polymorphism ◽  
Restriction Fragment ◽  
Length Polymorphism ◽  
Fragment Length ◽  
Fragment Length Polymorphism ◽  
Soybean Germplasm ◽  
Restriction Fragment Length
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