Cloning and characterisation of a new rye-specific repeated sequence

Genome ◽  
1991 ◽  
Vol 34 (1) ◽  
pp. 81-87 ◽  
Author(s):  
François Guidet ◽  
Peter Rogowsky ◽  
Christopher Taylor ◽  
Weining Song ◽  
Peter Langridge
Keyword(s):  
Molecular Marker ◽  
Dna Sequence ◽  
Repetitive Dna ◽  
Genetic Material ◽  
Repeated Sequence ◽  
Repeated Dna ◽  
New Family ◽  
Repetitive Dna Sequence ◽  
Repeated Dna Families

In this paper we describe a repetitive DNA sequence unique to the rye genome. The corresponding DNA probe (pAW173) was cloned as a 450-bp fragment and does not hybridize to wheat or barley DNA and is not related to five other repeated DNA families characterised in rye. This new family (designated the R173 family) consists of moderately repeated DNA (~ 15 000 units per genome) and is present on all seven rye chromosomes. The hybridization patterns in Southern blots indicate that it is not organised as blocks of tandem arrays and in situ hybridization shows that it is dispersed throughout the rye chromosomes. The probe has been successfully used as a molecular marker in identifying rye genetic material in a wheat background.Key words: repetitive DNA sequence, rye-specific DNA, molecular marker.

Characterization of a dispersed repetitive DNA sequence associated with the CCDD genome of wild rice

Genome ◽  
1995 ◽  
Vol 38 (4) ◽  
pp. 681-688 ◽  
Author(s):  
M. C. Kiefer-Meyer ◽  
A. S. Reddy ◽  
M. Delseny
Keyword(s):  
Nucleotide Sequence ◽  
Dna Sequence ◽  
Repetitive Dna ◽  
Dna Sequences ◽  
Wild Rice ◽  
Repeated Sequence ◽  
Amino Acid Sequences ◽  
Open Reading Frames ◽  
Repetitive Dna Sequence ◽  
Wild Rice Species

A HindII repetitive fragment (pOD3) was isolated and cloned from the genomic DNA of an accession of Oryza latifolia, a wild rice species that possesses a tetraploid CCDD genome. Southern blot analysis using this clone as a probe demonstrated that this repetitive DNA sequence had a dispersed organization in the CCDD genome and seemed to be highly specific for this genome type. This fragment is the first CCDD-specific repeated DNA sequence to be described. The hybridization pattern is similar for most CCDD accessions tested, although a few showed no hybridization signal. The nucleotide sequence of the element cloned in pOD3 was determined and analysed. The 1783 base pair long repeated sequence shows no homology with other known nucleotide sequences. In addition, none of the amino acid sequences deduced from the potential open reading frames contained in the pOD3 repeat is homologous to any known protein. The nucleotide sequence presents several internal repeats, direct or inverted, but their significance remains unknown.Key words: rice, dispersed repetitive DNA sequences, genome-specific sequences.

scholarly journals Characterisation of a novel repetitive DNA sequence fromMycobacterium bovis

1992 ◽  
Vol 96 (2-3) ◽  
pp. 179-185 ◽  
Author(s):  
T.J. Doran ◽  
A.L.M. Hodgson ◽  
J.K. Davies ◽  
A.J. Radford
Keyword(s):  
Dna Sequence ◽  
Repetitive Dna ◽  
Repetitive Dna Sequence

scholarly journals C-Ag+−C based repetitive DNA sequence

2020 ◽  
Vol 1679 ◽  
pp. 022049
Author(s):  
M A Popova ◽  
V I Rolich ◽  
R R Ramazanov ◽  
N A Kasyanenko ◽  
P A Sokolov
Keyword(s):  
Dna Sequence ◽  
Repetitive Dna ◽  
Repetitive Dna Sequence

scholarly journals Functional role of a highly repetitive DNA sequence in anchorage of the mouse genome

1988 ◽  
Vol 16 (17) ◽  
pp. 8351-8360 ◽  
Author(s):  
Beatrice Neuer-Nitsche ◽  
Xiang Lu ◽  
Dieter Werner
Keyword(s):  
Dna Sequence ◽  
Repetitive Dna ◽  
Functional Role ◽  
Mouse Genome ◽  
Repetitive Dna Sequence ◽  
Highly Repetitive Dna

A highly repetitive DNA sequence possibly unique to canids

Gene ◽  
1992 ◽  
Vol 110 (2) ◽  
pp. 235-238 ◽  
Author(s):  
Michael F. Minnick ◽  
Lisa C. Stillwell ◽  
Jill M. Heineman ◽  
Gary L. Stiegler
Keyword(s):  
Dna Sequence ◽  
Repetitive Dna ◽  
Repetitive Dna Sequence ◽  
Highly Repetitive Dna

scholarly journals The nucleotide sequence of the ubiquitous repetitive DNA sequence B1 complementary to the most abundant class of mouse fold-back RNA

1980 ◽  
Vol 8 (6) ◽  
pp. 1201-1216 ◽  
Author(s):  
A.S. Krayev ◽  
D.A. Kramerov ◽  
K.G. Skryabin ◽  
A.P. Ryskov ◽  
A.A. Bayev ◽  
...  
Keyword(s):  
Nucleotide Sequence ◽  
Dna Sequence ◽  
Repetitive Dna ◽  
Repetitive Dna Sequence
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