Characterization of MboI satellites in Cirrhina mrigala and Clarias batrachus (Pisces)

Genome ◽  
1998 ◽  
Vol 41 (1) ◽  
pp. 34-39 ◽  
Author(s):  
Bhaja K Padhi ◽  
Sudip K Ghosh ◽  
Radha K Mandal
Keyword(s):  
Parental Species ◽  
Labeo Rohita ◽  
Clarias Gariepinus ◽  
Clarias Batrachus ◽  
Catla Catla ◽  
Genetic Introgression ◽  
Heteropneustes Fossilis ◽  
Reciprocal Hybrids ◽  
Species Specific

We have cloned and characterized two highly reiterated, tandemly repeated, and A + T rich MboI DNA fragments, one in Cirrhina mrigala (Cyprinidae), with a monomer size of 266 bp, and one in Clarias batrachus (Clariidae), with a monomer size of 227 bp. The MboI fragment in C. mrigala is species-specific and absent in other carps, such as Catla catla and Labeo rohita. The MboI fragment in C. batrachus was also present in two other catfishes tested, namely Clarias gariepinus and Heteropneustes fossilis. In C. mrigala xC. catla and C. mrigala x L. rohita hybrids, the C. mrigala specific MboI fragment is inherited uniparentally. In the reciprocal hybrids of C. batrachus xH. fossilis, the satellite ladder contains the bands of both parental species. The MboI satellite of carp may be useful in genetic introgression analysis and that of catfish in distinguishing between gynogenetic progeny and true hybrids.

Development and characterization of cell lines derived from rohu,Labeo rohita(Hamilton), and catla,Catla catla(Hamilton)

2009 ◽  
Vol 32 (3) ◽  
pp. 211-218 ◽  
Author(s):  
V P Ishaq Ahmed ◽  
V Chandra ◽  
R Sudhakaran ◽  
S Rajesh Kumar ◽  
M Sarathi ◽  
...  
Keyword(s):  
Cell Lines ◽  
Labeo Rohita ◽  
Catla Catla

scholarly journals ECTO- PARASITISM IN JUVENILE INDIAN MAJOR CARPS OF DIFFERENT FISH FARMS OF MYMENSINGH

2017 ◽  
Vol 14 (2) ◽  
pp. 257-262
Author(s):  
D. R. Das ◽  
S. Majumder ◽  
K. J. Chandra
Keyword(s):  
Labeo Rohita ◽  
Quality Parameters ◽  
Management Systems ◽  
Catla Catla ◽  
Fish Farms ◽  
Indian Major Carps ◽  
Species Specific ◽  
Fish Hosts ◽  
Major Carps ◽  
Eleven Species

An investigation was made on the ecto-parasitism of juvenile carps of Mymensingh region.  Eight  farms, four Government (Govt.) and four Private (Pvt.) fish farms were investigated for Indian major carps(Catla catla, Labeo rohita and Cirrhinus cirrhosus)  (Catla, Rui and Mrigal)  during  June, 2010 to May, 2012. Monthly samplings were carried out with 5 fish from each species and each farms. Altogether 2880 fish hosts were examined of which 1674 were found to be infested with different groups of parasites during the study period. The ectoparasites were very common in gill, skin, mouth cavity, muscle, base of the pelvic, pectoral, anal, caudal fin and body surface of all fishes. Eleven species of protozoan, thirteen species of  monogeneans and two species of argulus were recorded. The risk of being infestation by parasites in carps significantly (p<0.001) increased when the water quality parameters were deteriorated. Prevalence (%), mean intensity and abundance were found to be species specific and also varied with seasons and management systems practiced by different farmers. Prevalence (%) of ecto-parasites in carps was significantly (p< 0.001) higher in Pvt. farm than that of Govt. farms and was significantly (p< 0.01) higher in rainy season. Rui was more susceptible than Catla and Mrigal.

Morphological Redescription and Molecular Characterization of Dactylogyrus Labei (Monogenea, Dactylogyridae) from Catla Catla: A New Host Record in India

2014 ◽  
Vol 48 (5) ◽  
pp. 451-456 ◽  
Author(s):  
H. R. Chiary ◽  
A. Chaudhary ◽  
H. S. Singh
Keyword(s):  
Molecular Characterization ◽  
West Bengal ◽  
Labeo Rohita ◽  
Host Record ◽  
First Record ◽  
Catla Catla ◽  
New Host ◽  
Atypical Form ◽  
New Host Record

Abstract Morphological Redescription and Molecular Characterization of Dactylogyrus labei (Monogenea, Dactylogyridae) from Catla catla: a New Host Record in India. Chiary, H. R., Chaudhary, A., Singh, H. S. - Dactylogyrus labei Musselius et Gusev, 1976 (atypical form) specimens were collected from host Catla catla from the river Brahmaputra, Guwahati, Assam, India. The Dactylogyrus samples were examined by morphological and molecular methods. This parasite was originally described from Labeo rohita from fish farm Kalyani, West Bengal, India, but the morphological study of sclerotized parts showed the existence of D. labei on Catla catla. This is the first record of D. labei from Catla catla in India.

scholarly journals Livelihood Patterns and Socio-economic Conditions of the Fishermen of Kharingcha Baor at Chaugachha Upazilla in Jashore District, Bangladesh

2021 ◽  
pp. 61-74
Author(s):  
Md. Raihan Uddin ◽  
M. Aminur Rahman ◽  
Moumita Choudhury ◽  
Md. Almamun Farid ◽  
Anusree Biswas ◽  
...  
Keyword(s):  
Cyprinus Carpio ◽  
Labeo Rohita ◽  
Economic Conditions ◽  
Small Scale ◽  
Catla Catla ◽  
Sustainable Livelihood ◽  
Heteropneustes Fossilis ◽  
Socioeconomic Condition ◽  
Channa Striatus ◽  
Village Doctors

A field study was carried out to investigate the livelihood patterns the socio-economic conditions of the small-scale traditional fishermen of Kharingcha baor at Chaugachha upazilla in Jashore, Bangladesh. The survey was conducted on 100 peoples from 70 families of the fishermen in the study area. It was found from the study that 94% fishermen were comprising of males, while 6% were of females. Among them, the majority was Hindus (88%) and the minority was Muslims (12%). During the suvey, the proportion of semi paka houses were documented to be 88%, while kancha were 12%, and all the families had electricity facilities. It was found that 20% of fishermen had no education, 6% had passed SSC-HSC examinations, 14% had passed 6-10 classes and 24% had passed 1-5 classes. It revealed that 84% of fishermen were married and 16% were unmarried. Throughout the study, 33% of the fishermen were found to be dependent on village doctors, 40% on upazilla hospital and 27% on MBBS doctors. Several types of local boats and dingi nouka as well as nets such as tana jal (surrounding net), set bagnet, khapla jal (cast net), thela jal (push net) etc. were used for the harvesting of different fish species (Labeo rohita, Catla catla, Cyprinus carpio, Cirrhinus mrigala, Heteropneustes fossilis, Channa striatus, C. punctatus etc.). Averagely, 35-80 kg fish per day was caught by a group of fishermen using a boat. The majority of the fishers sold their harvested fishes upon the fulfillment of their demands. The monthly mean income of fishermen was around BDT. 5,000-15,000 in the baor area. Among the fishermen, 56% of them were observed to lend money from money lenders and 44% from the NGOs for their fishing-related activities and livelihoods. It revealed from the present findings that the traditional fishermen of Kharingcha baor require more technical, organizational and institutional helps for the improvement of their socioeconomic condition and sustainable livelihood.

scholarly journals Cannibalism and Performance Evaluation of Hybrids between Clarias batrachus and Clarias gariepinus

2016 ◽  
Vol 74 (3) ◽  
pp. 124-129 ◽  
Author(s):  
Samuel Olabode Olufeagba ◽  
Victor Tosin Okomoda
Keyword(s):  
Performance Evaluation ◽  
Growth Performance ◽  
Clarias Gariepinus ◽  
Commercial Production ◽  
Clarias Batrachus ◽  
African Catfish ◽  
Excellent Performance ◽  
Reciprocal Hybrids ◽  
And Performance

AbstractCannibalism is an important characteristic of African catfish that significantly reduces the number of stocked fish at harvest. This study evaluates the effect of reciprocal hybridization of Clarias batrachus and Clarias gariepinus on cannibalism and growth performance of their progenies in an indoor rearing system. The result obtained reveals excellent performance of hybrids of C. gariepinus ♂ and C. batrachus ♀ over the purebreds in terms of growth. However, the reciprocal hybrids between C. gariepinus ♀ and C. batrachus ♂ all died few hours after hatching. Cannibalism was significantly reduced in the crosses between C. gariepinus ♂ and C. batrachus ♀ with 100% survival after five months of culture. Hence, this could be exploited in commercial production of catfish to reduce cannibalism and increase harvest size of fish farmers.

Incidence and molecular characterization of fungi and yeast isolated from cultured catfish and Nile tilapia

2020 ◽  
Vol 21 (3) ◽  
pp. 61-66
Author(s):  
Ola Hashem ◽  
Viola Zaki ◽  
Rawia Adawy
Keyword(s):  
Molecular Characterization ◽  
Oreochromis Niloticus ◽  
Nile Tilapia ◽  
Clarias Gariepinus ◽  
Fungal Species ◽  
High Rate ◽  
Fish Farms ◽  
Isolation And Identification ◽  
Penicillium Spp

Objective: To study the incidence and seasonal dynamics of different fungi affected freshwater fishes in Lake Manzala with molecular identification of the isolated fungi. Animals: 300 Nile tilapia (Oreochromis niloticus) and 300 catfish (Clarias gariepinus). Design: Descriptive study. Procedures: Random samples of Oreochromis niloticus (O. niloticus) and Clarias gariepinus (C. gariepinus) were collected from Manzala fish farms. Clinical and postmortem examination of fish was applied. Isolation and identification of different fungi were performed by conventional methods. Furthermore, the molecular characterization of isolated fungi was carried out. Results: C. gariepinus had a higher rate of infection with different fungal species than O. niloticus. Aspergillus spp. (Aspergillus niger and Aspergillus flavus) were the most fungal isolated from the examined fishes, followed by Penicillium spp. and Candida albicans. Aspergillus spp were detected in all seasons with a higher rate in summer and spring. A. flavus, A. niger, Penicillium spp. and C.albicans isolates were amplified from both C. gariepinus and O. niloticus at the specified molecular weight using PCR. Conclusion and clinical relevance: Fungal infection affected the fish showing different external and internal lesions, all species of Aspergillus were found in all seasons with a high rate in, hot seasons, summer and spring. The Prevalence of Penicillium and C. albicans were also reported. All fungal isolates were identified on the phenotypic and molecular bases.

scholarly journals Characterization of the Major Antigenic Protein 2 of Ehrlichia canis and Ehrlichia chaffeensis and Its Application for Serodiagnosis of Ehrlichiosis

2003 ◽  
Vol 10 (4) ◽  
pp. 520-524 ◽  
Author(s):  
Tamece T. Knowles ◽  
A. Rick Alleman ◽  
Heather L. Sorenson ◽  
David C. Marciano ◽  
Edward B. Breitschwerdt ◽  
...  
Keyword(s):  
Blot Analysis ◽  
Single Copy ◽  
Ehrlichia Canis ◽  
Specific Method ◽  
Ehrlichia Chaffeensis ◽  
Antigenic Protein ◽  
Canine Monocytic Ehrlichiosis ◽  
Copy Gene ◽  
Species Specific

ABSTRACT Canine monocytic ehrlichiosis, caused by Ehrlichia canis or Ehrlichia chaffeensis, can result in clinical disease in naturally infected animals. Coinfections with these agents may be common in certain areas of endemicity. Currently, a species-specific method for serological diagnosis of monocytic ehrlichiosis is not available. Previously, we developed two indirect enzyme-linked immunosorbent assays (ELISAs) using the major antigenic protein 2 (MAP2) of E. chaffeensis and E. canis. In this study, we further characterized the conservation of MAP2 among various geographic isolates of each organism and determined if the recombinant MAP2 (rMAP2) of E. chaffeensis would cross-react with E. canis-infected dog sera. Genomic Southern blot analysis using digoxigenin-labeled species-specific probes suggested that map2 is a single-copy gene in both Ehrlichia species. Sequences of the single map2 genes of seven geographically different isolates of E. chaffeensis and five isolates of E. canis are highly conserved among the various isolates of each respective ehrlichial species. ELISA and Western blot analysis confirmed that the E. chaffeensis rMAP2 failed to serologically differentiate between E. canis and E. chaffeensis infections.

Characterization of trypanosome isolates from cattle in Uganda using species-specific DNA probes reveals predominance of mixed infections

1990 ◽  
Vol 11 (03) ◽  
pp. 271-280
Author(s):  
J. H. P. Nyeko ◽  
O. K. Ole-Moiyoi ◽  
P. A. O. Majiwa ◽  
L. H. Otieno ◽  
P. M. Ociba
Keyword(s):  
Dna Probes ◽  
Mixed Infections ◽  
Species Specific
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