The effects of increased endurance training load on biomarkers of heat intolerance during intense exercise in the heat

2009 ◽  
Vol 34 (4) ◽  
pp. 616-624 ◽  
Author(s):  
Chin Leong Lim ◽  
David Pyne ◽  
Peggy Horn ◽  
Amelia Kalz ◽  
Philo Saunders ◽  
...  
Keyword(s):  
Tumor Necrosis ◽  
Venous Blood ◽  
Plasma Concentrations ◽  
Training Load ◽  
Training Intervention ◽  
Intense Exercise ◽  
Short Term ◽  
Volitional Exhaustion ◽  
Tnf Α ◽  
Necrosis Factor

The effects of increased training (IT) load on plasma concentrations of lipopolysaccharides (LPS), proinflammatory cytokines, and anti-LPS antibodies during exercise in the heat were investigated in 18 male runners, who performed 14 days of normal training (NT) or 14 days of 20% IT load in 2 equal groups. Before (trial 1) and after (trial 2) the training intervention, all subjects ran at 70% maximum oxygen uptake on a treadmill under hot (35 °C) and humid (∼40%) conditions, until core temperature reached 39.5 °C or volitional exhaustion. Venous blood samples were drawn before, after, and 1.5 h after exercise. Plasma LPS concentration after exercise increased by 71% (trial 1, p < 0.05) and 21% (trial 2) in the NT group and by 92% (trial 1, p < 0.01) and 199% (trial 2, p < 0.01) in the IT group. Postintervention plasma LPS concentration was 35% lower before exercise (p < 0.05) and 47% lower during recovery (p < 0.01) in the IT than in the NT group. Anti-LPS IgM concentration during recovery was 35% lower in the IT than in the NT group (p < 0.05). Plasma interleukin (IL)-6 and tumor necrosis factor (TNF)-α concentrations after exercise (IL-6, 3−7 times, p < 0.01, and TNF-α, 33%, p < 0.01) and during recovery (IL-6, 2−4 times, p < 0.05, and TNF-α, 30%, p < 0.01) were higher than at rest within each group. These data suggest that a short-term tolerable increase in training load may protect against developing endotoxemia during exercise in the heat.

Physiological persona differences based on stress and inflammation between meditators and healthy controls

2019 ◽  
Vol 17 (2) ◽  
Author(s):  
Dipti Magan ◽  
Raj Kumar Yadav
Keyword(s):  
Blood Pressure ◽  
Body Mass Index ◽  
Plasma Levels ◽  
Inflammatory Markers ◽  
Tumor Necrosis ◽  
Healthy Controls ◽  
Short Term ◽  
Tnf Α ◽  
Necrosis Factor

AbstractBackgroundNowadays, yoga is endorsed and advised routinely to stay fit and healthy, as well as control many chronic diseases including diabetes type 2, hypertension, coronary artery diseases, etc. Now, our assumption is that those who do regular yoga have different persona than who do not do yoga regularly. We planned to test our hypothesis scientifically, and therefore baseline physiological characteristics with stress and inflammation levels in long-term and short-term meditators and healthy novice controls were analyzed.MethodsIn this retrospective analysis, 97 male participants were included for their Baseline analysis. Fifteen apparently healthy subjects practicing preksha meditation (since >5 years, at least 5 days a week) were included as long-term meditators (LTMs); 58 subjects who attended one of our short-term yoga-based lifestyle intervention programs for 2 weeks were included as short-term meditators (STMs); 24 male novice subjects, who did not participate in any yogic intervention, were included as healthy controls. Here, we analyzed the Baseline plasma levels of stress and inflammatory markers, cortisol, β-endorphin, interleukin (IL)-6 and tumor necrosis factor (TNF)-α in long-term meditators vs. short-term meditators vs. healthy controls.Outcome measuresThe study parameters body mass index (BMI), systolic blood pressure (SBP), diastolic blood pressure (DBP), plasma levels of stress and immune markers, cortisol, β-endorphin (β-Ed), interleukin-6 (IL-6) and tumor necrosis factor-α (TNF-α), were assessed in all the three groups at baseline.ResultsSignificant (p<0.05) differences were observed at baseline for plasma levels of stress and inflammatory markers as well as body mass index and systolic blood pressure among LTM vs. STM vs. healthy controls.ConclusionsOur observations suggest that the subjects who do regular yoga-meditation practice have better stress & inflammation status than comparable age matched healthy controls.

scholarly journals Short-term alpha- or gamma-delta-enriched tocopherol oil supplementation differentially affects the expression of proinflammatory mediators: selective impacts on characteristics of protein tyrosine nitration in vivo

2013 ◽  
Vol 3 (1) ◽  
pp. 6 ◽  
Author(s):  
Ted H. Elsasser ◽  
Stanislaw Kahl ◽  
Katie M. Lebold ◽  
Maret G. Traber ◽  
Jessica Shaffer ◽  
...  
Keyword(s):  
Plasma Concentrations ◽  
Short Term ◽  
Proinflammatory Response ◽  
E Coli ◽  
Amyloid A ◽  
Proinflammatory Mediators ◽  
Tnf Α ◽  
Factor Α ◽  
Necrosis Factor

While vitamin E has been used for decades in cattle diets, the principle form used traditionally is the synthetic α-isoform acetate or succinate and largely no data exist on the biological partitioning or functionality of the major naturally occurring γ- and δ-isoforms in cattle. Using tyrosine 3’-nitrated protein (pNT) as a biomarker of nitrosative cell stress, we sought to evaluate the effectiveness of short-term feeding supplementation of high content natural α-tocopherol (<em>α-T</em>, 96% α-isomer) compared to high content γ- and δ-enriched low α-content mixed tocopherol oils (<em>γ-T</em>, ~70% <em>γ-</em>, 20% δ-, &lt;5% α-isoform) to mitigate systemic and hepatic aspects of the proinflammatory response to endotoxin (LPS). Calves fed diets supplemented with <em>α-T</em>, <em>γ-T</em> for five days or no tocopherol supplement (<em>T0E</em>) were challenged with a low-level of LPS (0.25 μg/kg, iv, <em>E. coli </em>055:B5) sufficient to effect a liver nitration response. As fed,<em> α-T</em> or <em>γ-T</em> increased plasma and liver content of the respective tocopherols reflecting their relative abundance in the respective diets. Plasma or tissue mediators and biomarkers of the proinflammatory response [plasma concentrations of tumor necrosis factor-α (TNF-α, P&lt;0.001), nitrate+nitrite (NOx, P&lt;0.01), and serum amyloid A (SAA, P&lt;0.001)], and general liver content of pNT (P&lt;0.005) increased after LPS. LPS-mediated increases in TNF-α were not dif- ferent between diet treatments; both plasma NOx (P&lt;0.05) and generalized liver pNT (P&lt;0.03) responses were attenuated significantly in <em>α-T </em>and <em>γ-T versus T0E calves</em>. Plasma SAA was significantly decreased in γ-T calves at 24 h post-LPS relative to responses in <em>α-T</em> or <em>T0E </em>calves. The nitration of the mitochondrial proteins 24 h post-LPS was not only attenuated in <em>α-T</em> and <em>γ-T vs T0E</em>, but also the mitigating effect of <em>γ-T</em> on these specific nitration events was greater than that of <em>α-T </em>(P&lt;0.01). Results are consistent with the concept that short-term <em>α-T</em> or <em>γ-T</em> supplementation can effectively decrease proinflammatory liver pNT after LPS; some mitochondrial nitration targets may be better protected with prophylactic supplementation with γ-,δ-tocopherol enriched oil.

Brain-blood permeability: TNF-α promotes escape of protein tracer from CSF to blood

2000 ◽  
Vol 279 (1) ◽  
pp. R148-R151 ◽  
Author(s):  
Jodi B. Dickstein ◽  
Harvey Moldofsky ◽  
John B. Hay
Keyword(s):  
Central Nervous System ◽  
Cerebrospinal Fluid ◽  
Nervous System ◽  
Tumor Necrosis ◽  
Venous Blood ◽  
Protein Clearance ◽  
Tracer Recovery ◽  
Tnf Α ◽  
The Central Nervous System ◽  
Necrosis Factor

The objective of this study was to determine the effect of tumor necrosis factor (TNF)-α on the efflux of protein from the central nervous system to blood based on assessing the clearance of radiolabeled albumin from the cerebrospinal fluid (CSF) to blood in rats. 125I-labeled human serum albumin (125I-HSA) was injected into a lateral ventricle, and venous blood was sampled hourly to determine the basal CSF protein clearance into the blood. After this, rats were intraventricularly infused with 10 μl TNF-α and 10 μl131I-HSA ( n = 6) or 10 μl saline and 10 μl 131I-HSA ( n = 6). Venous blood was sampled hourly for 3 h. 131I-HSA tracer recovery increased threefold in the venous blood and was significantly higher in the spleen, muscles, and skin in animals treated with TNF-α. No significant changes were observed in control animals treated with saline. The data suggest that TNF-α promotes the clearance of protein macromolecules from the CSF to the venous blood.

scholarly journals Analysis of Bone Marrow-derived Mesenchymal Stem Cell Kinetics after Short-term Stimulation with Tumor Necrosis Factor-α (TNF-α)

2019 ◽  
Vol 28 (2) ◽  
pp. 99-108 ◽  
Author(s):  
Mio Naritani ◽  
Miho Inoue ◽  
Resmi Raju ◽  
Mayu Miyagi ◽  
Masamitsu Oshima ◽  
...  
Keyword(s):  
Bone Marrow ◽  
Stem Cell ◽  
Tumor Necrosis Factor ◽  
Tumor Necrosis ◽  
Tumor Necrosis Factor Α ◽  
Cell Kinetics ◽  
Short Term ◽  
Tnf Α ◽  
Factor Α ◽  
Necrosis Factor

Factors mediating the hemodynamic effects of tumor necrosis factor-α in portal hypertensive rats

1999 ◽  
Vol 276 (3) ◽  
pp. G687-G693 ◽  
Author(s):  
Javier Muñoz ◽  
Agustín Albillos ◽  
María Pérez-Páramo ◽  
Irma Rossi ◽  
Melchor Alvarez-Mon
Keyword(s):  
Nitric Oxide ◽  
Tumor Necrosis ◽  
Tumor Necrosis Factor Α ◽  
Portal Pressure ◽  
Systemic Resistance ◽  
Short Term ◽  
Tnf Α ◽  
Factor Α ◽  
Necrosis Factor

Nitric oxide, prostacyclin, and glucagon have been implicated in promoting the hyperdynamic circulatory state of portal hypertension. Recent evidence also indicates that increased tumor necrosis factor-α (TNF-α) production is involved in the pathogenesis of this hemodynamic abnormality. This study was aimed at investigating in rats with portal vein stenosis (PVS) the effects on splanchnic hemodynamics of blocking circulating TNF-α and the factors mediating the vascular action of this cytokine in this setting. Anti-TNF-α polyclonal antibodies or placebo was injected into rats ( n = 96) before and 4 days after PVS (short-term inhibition) and at 24 h and 4, 7, 10 days after PVS (long-term inhibition). Short-term TNF-α inhibition reduced portal venous inflow and cardiac index and increased splanchnic and systemic resistance. Portal pressure was unchanged, but portal-systemic shunting was decreased. After long-term TNF-α inhibition, portal venous inflow and portal pressure were unchanged, but arterial pressure and systemic resistance rose significantly. Anti-TNF-α PVS rats exhibited lower increments of systemic resistance after N ω-nitro-l-arginine methyl ester and indomethacin administration and lower serum levels of TNF-α, nitrates-nitrites, and 6-keto-PGF1α, both over the short and the long term. Serum glucagon levels rose after long-term inhibition. In conclusion, the specific role played by TNF-α in the development of the hyperdynamic state of portal hypertension appears to be mainly mediated through an increased release of nitric oxide and prostacyclin. Maintenance of the splanchnic hyperemia after long-term TNF-α inhibition could be due to a compensatory release of glucagon.

Changes in Serum Tumor Necrosis Factor (TNF-α) with Kami-Shoyo-San Administration in Depressed Climacteric Patients

2004 ◽  
Vol 32 (04) ◽  
pp. 621-629 ◽  
Author(s):  
Takahisa Ushiroyama ◽  
Atsushi Ikeda ◽  
Kou Sakuma ◽  
Minoru Ueki
Keyword(s):  
Tumor Necrosis Factor ◽  
Herbal Medicine ◽  
Tumor Necrosis ◽  
Rating Scale ◽  
Therapy Group ◽  
Plasma Concentrations ◽  
Herbal Medicines ◽  
College Hospital ◽  
Tnf Α ◽  
Necrosis Factor

An herbal medicine (Kampo) is widely used to prevent or treat climacteric symptoms. In order to investigate the potential involvement of tumor necrosis factor (TNF)-α in susceptibility to mood disorder in climacteric women and to clarify the relationship between immune function and the efficacy of herbal medicine, we compared serum TNF-α levels in two treated groups, with and without concurrent use of herbal medicine. This study included 113 consecutive depressed menopausal patients who visited the gynecological and psychosomatic medicine outpatient clinic of the Osaka Medical College Hospital in Japan. Fifty-eight patients were administered Kami-shoyo-san according to the definition of above Sho. In contrast, 55 patients who were different in Sho of Kami-shoyo-san were administered antidepressants. Hamilton Rating Scale for depression (HAM-D) scores were determined at baseline and 12 weeks after starting treatment (endpoint). TNF-α concentrations were analyzed before and after 12 weeks of treatment. Kami-shoyo-san significantly increased plasma concentrations of TNF-α after 12 weeks of treatment, to 17.22 ± 6.13 pg/ml from a baseline level of 14.16 ± 6.27 pg/ml (p = 0.048). The percent change in plasma concentration of TNF-α differed significantly between the Kami-shoyo-san therapy group and the antidepressant therapy group at 4 weeks (12.0 ± 7.8% and -1.22 ± 0.25%, respectively, p <0.01), 8 weeks (19.7 ± 3.4% and -2.45 ± 0.86%, respectively, p <0.01), and 12 weeks (21.3 ± 5.4% and -6.81 ± 2.2%, respectively, p <0.001). We found in this study that Kami-shoyo-san, an herbal medicine, increased plasma TNF-α levels in depressed menopausal patients. Cytokines may play various roles in mood and emotional status via the central nervous system and may be regulated by herbal medicines, although the interactions are very complex.

scholarly journals EFFECTS OF A PROBIOTIC MILK DRINK ON SALIVARY TUMOR NECROSIS FACTOR-ALPHA IN CHILDREN WITH ACUTE LYMPHOBLASTIC LEUKEMIA IN THE INDUCTION PHASE

2018 ◽  
Vol 11 (2) ◽  
pp. 340
Author(s):  
Jennifer Surja Pariaputra ◽  
Sarworini Bagio Budiardjo ◽  
Margaretha Suharsini
Keyword(s):  
Tumor Necrosis ◽  
Lymphoblastic Leukemia ◽  
Control Group ◽  
Induction Phase ◽  
Short Term ◽  
Necrosis Factor Alpha ◽  
Tnf Α ◽  
Factor Alpha ◽  
Experimental Group ◽  
Necrosis Factor

 Objective: The aim of this study was to investigate the effects of the short-term use of a probiotic milk drink on salivary tumor necrosis factor-alpha (TNF-α) in children with acute lymphoblastic leukemia (ALL) during the induction phase of chemotherapy.Methods: A randomized, case-controlled clinical trial was carried out. The samples of the study were collected from 11 children with ALL and 11 healthy children as the control. Both groups received a probiotic milk drink. The probiotic milk drink contained Lactobacillus casei (6.5×109 colony forming unit/mL). The subjects were instructed to rinse 5 mL of probiotic milk drink for 30 s over the course of 2 weeks. Unstimulated saliva samples were collected from patients at baseline and 1 and 2 weeks after rinsing with the probiotic milk drink. The levels of TNF-α were determined using a human TNF-α enzyme-linked immunosorbent assay kit.Results: The salivary TNF-α levels in the experimental group decreased but not to a statistically significant degree (p>0.05) when comparing the baseline with weeks 1 and 2 after rinsing. There was a significant decrease of salivary TNF-α levels in the control group (p<0.05) when comparing the baseline with 1 and 2 weeks after rinsing. The concentration of salivary TNF-α in the experimental group was higher than in the control group (p<0.05).Conclusion: This study demonstrates the effect of a short-term intake of a probiotic milk drink on children with ALL during the induction phase of chemotherapy. The results show a reduction of salivary TNF-α after taking the probiotic milk drink after 1 week and 2 weeks, although the results were not statistically significant.

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