Sources of alcohol dehydrogenase (NAD+-linked) in the cockroach, Leucophaea maderae

1969 ◽  
Vol 15 (3) ◽  
pp. 257-258 ◽  
Author(s):  
Carl W. Despreaux Jr. ◽  
Leon L. Pierre
Keyword(s):  
Alcohol Dehydrogenase ◽  
Enzyme Synthesis ◽  
Normal Tissues ◽  
Leucophaea Maderae ◽  
Fat Bodies

The activity of alcohol dehydrogenase (NAD+-linked) in symbionts, and normal and aposymbiotic fat bodies of the (Madeira) cockroach, Leucophaea maderae, revealed the following: (1) highest concentrations of the enzyme in symbiont materials; (2) lowest concentrations of the enzyme in aposymbiotic fat bodies; (3) comparable concentrations of the enzyme in similar types of fat bodies; (4) possible symbiont contribution of the enzyme to normal insects; and (5) probable inhibition of the symbiont enzyme synthesis in normal tissues.

Tyrosinase activity of the symbionts and fat bodies of the cockroach, Leucophaea maderae

1969 ◽  
Vol 15 (3) ◽  
pp. 253-255 ◽  
Author(s):  
Charles Goldberg ◽  
Leon L. Pierre
Keyword(s):  
Enzyme Synthesis ◽  
Tyrosinase Activity ◽  
Insect Cuticle ◽  
Leucophaea Maderae ◽  
Fat Bodies

Tyrosinase activity of the symbionts, and fat bodies of the (Madeira) cockroach, Leucophaea maderae, revealed the following: (a) greatest enzyme synthesis (which decreased upon adolescence) by normal nymphal fat bodies; (b) decreased and somewhat comparable synthesis by aposymbiotic fat bodies; (c) least synthesis by symbiont materials; and (d) definite symbiont enzyme contribution to the pigmentation of the normal insect cuticle.

Malic Enzyme Activity in the Fat Bodies of the Cockroach, Leucophaea maderae (F.)

Nature ◽  
1966 ◽  
Vol 210 (5042) ◽  
pp. 1293-1294 ◽  
Author(s):  
NATHAN DUBOWSKY ◽  
LEON L. PIERRE
Keyword(s):  
Enzyme Activity ◽  
Malic Enzyme ◽  
Leucophaea Maderae ◽  
Malic Enzyme Activity ◽  
Fat Bodies

Cytosolic and nuclear receptors for juvenile hormone in fat bodies of Leucophaea maderae

1987 ◽  
Vol 17 (7) ◽  
pp. 1045-1052 ◽  
Author(s):  
Franz Engelmann ◽  
Jaroslava Mala ◽  
Stephen S. Tobe
Keyword(s):  
Juvenile Hormone ◽  
Nuclear Receptors ◽  
Leucophaea Maderae ◽  
Fat Bodies

scholarly journals GENETIC VARIATION IN THE EXPRESSION OF ADH IN DROSOPHILA MELANOGASTER

Genetics ◽  
1982 ◽  
Vol 101 (3-4) ◽  
pp. 431-446
Author(s):  
G Maroni ◽  
C C Laurie-Ahlberg ◽  
D A Adams ◽  
A N Wilton
Keyword(s):  
Drosophila Melanogaster ◽  
Genetic Variation ◽  
Alcohol Dehydrogenase ◽  
Structural Gene ◽  
Alimentary Canal ◽  
Fat Body ◽  
Natural Populations ◽  
Enzyme Protein ◽  
Fat Bodies ◽  
Highly Correlated

ABSTRACT Several chromosomes derived from natural populations have been identified that affect the expression of alcohol dehydrogenase (ADH) . Second chromosomes, which also carry the structural gene Adh, show a great deal of polymorphism of genetic elements that determine how much enzyme protein accumulates. The level of enzyme was measured in third instar larvae, 6-to-8-day-old males and in larval fat bodies and alimentary canals. In general, activities in the different organs and stages are highly correlated with one another. One line was found, however, in which the ADH level in the fat body is more than twice the level one would expect on the basis of the activity in alimentary canal. We have also found evidence of third-chromosome elements that affect the level of ADH.

Activity of Isocitric Dehydrogenase in the Fat Bodies of the Cockroach, Leucophaea maderae (F)

Nature ◽  
1967 ◽  
Vol 213 (5072) ◽  
pp. 209-210 ◽  
Author(s):  
NATHAN DUBOWSKY ◽  
LEON L. PIERRE
Keyword(s):  
Isocitric Dehydrogenase ◽  
Leucophaea Maderae ◽  
Fat Bodies

Surface Ultrastructure of Human Ectocervix in Certain Pathological Conditions

1985 ◽  
Vol 43 ◽  
pp. 570-571
Author(s):  
S. Mukherjee ◽  
T. Guha ◽  
B. Chakrabarti ◽  
P. Chakrabarti
Keyword(s):  
Epithelial Cells ◽  
Marked Reduction ◽  
Squamous Epithelium ◽  
Malignant Tumour ◽  
Surface Ultrastructure ◽  
Normal Tissues ◽  
Pathological Conditions ◽  
Hexagonal Shape ◽  
Columnar Cells ◽  
Scanning Electron

The cervix is an important organ in reproduction. Its malfunction is frequently a factor for infertility. Ectocervix region does not appear to have received much attention although many studies have been reported on the endocervix. We report here our SEM observations on ectocervix in certain pathological conditions compared to normal ectocervix.Ectocervix specimens from human females with specific pathological disorders were processed for Scanning Electron Microscopy by conventional method and they were examined in a Philips SEM.The normal ectocervix is lined by flat layer of squamous epithelial cells with microridges (Fig. 1). These cells are known to be formed from columnar cells through metaplastic transformation. The cells of carcinoma-bearing ectocervix show a disorganised appearance (Fig. 2). In non-malignant tumour surface some cuboidal and few columnar cells were seen (Fig. 3). A cyst appears like an overgrowth on the surface of the squamous epithelium (Fig. 4). In ulcerated ectocervix a marked reduction of epithelial cells are observed (Fig. 5); the cells are devoid of microridges and, the large polygonal cells, as observed in normal tissues, have somehow acquired comparatively small hexagonal shape

Glucose metabolism in cancer cells: immunogold localization of hexokinase to the outer mitochondrial membrane

1995 ◽  
Vol 53 ◽  
pp. 1044-1045
Author(s):  
Krishan K. Arora ◽  
Glenn L. Decker ◽  
Peter L. Pedersen
Keyword(s):  
Tumor Cells ◽  
Mitochondrial Membrane ◽  
Present Report ◽  
Large Fraction ◽  
Mitochondrial Fraction ◽  
Immunogold Labeling ◽  
Outer Mitochondrial Membrane ◽  
Immunogold Localization ◽  
Hexokinase Activity ◽  
Normal Tissues

Hexokinase (ATP: D-hexose 6-phophotransferase EC 2.7.1.1) is the first enzyme of the glycolytic pathway which commits glucose to catabolism by catalyzing the phosphorylation of glucose with ATP. Previous studies have shown diat hexokinase activity is markedly elevated in rapidly growing tumor cells exhibiting high glucose catabolic rates. A large fraction (50-80%) of this enzyme activity is bound to the mitochondrial fraction (1,2) where it has preferred access to ATP (3). In contrast,the hexokinase activity of normal tissues is quite low, with one exception being brain which is a glucose-utilizing tissue (4). Biochemical evidence involving rigorous subfractionation studies have revealed striking differences between the subcellular distribution of hexokinase in normal and tumor cells [See review by Arora et al (4)].In the present report, we have utilized immunogold labeling techniques to evaluate die subcellular localization of hexokinase in highly glycolytic AS-30D hepatoma cells and in the tissue of its origin, i.e., rat liver.

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