Redox reactions of reactive oxygen species in aqueous solutions as the probe for scanning electrochemical microscopy of single live T24 cells

2010 ◽  
Vol 88 (6) ◽  
pp. 569-576 ◽  
Author(s):  
Xiaocui Zhao ◽  
Mengni Zhang ◽  
Yitao Long ◽  
Zhifeng Ding
Keyword(s):  
Reactive Oxygen Species ◽  
Hydrogen Peroxide ◽  
Aqueous Solutions ◽  
Redox Reactions ◽  
Reactive Oxygen ◽  
Scanning Electrochemical Microscopy ◽  
Electrochemical Technique ◽  
Oxygen Species ◽  
T24 Cells ◽  
Electrochemical Microscopy

The redox reactions of two main components of reactive oxygen species (ROS), superoxide and hydrogen peroxide, along with oxygen in aqueous solutions were investigated using a conventional electrochemical technique, differential pulse voltammetry (DPV). Superoxide undergoes oxidation at a Pt working electrode biased at 0.055 V versus Ag/AgCl, while hydrogen peroxide can be oxidized and reduced at 0.817 and –0.745 V, respectively. Oxygen in the solutions is reduced at the electrode with an applied potential of –0.455 V. Based on these results, hydrogen peroxide and superoxide released from live cells can be successfully monitored, identified, and mapped using scanning electrochemical microscopy (SECM) at different potentials. Single human bladder (T24) cells were imaged using a 5 μm diameter SECM probe biased at –0.400, –0.600, and –0.800 V. Oxygen reduction that seems an interference can be discriminated from that of hydrogen peroxide by means of SECM.

Scanning Electrochemical Microscopy Studies of Glutathione-Modified Surfaces. An Erasable and Sensitive-to-Reactive Oxygen Species Surface

Langmuir ◽  
2011 ◽  
Vol 27 (17) ◽  
pp. 11206-11211 ◽  
Author(s):  
Alina Latus ◽  
Jean-Marc Noël ◽  
Elena Volanschi ◽  
Corinne Lagrost ◽  
Philippe Hapiot
Keyword(s):  
Reactive Oxygen Species ◽  
Reactive Oxygen ◽  
Scanning Electrochemical Microscopy ◽  
Oxygen Species ◽  
Modified Surfaces ◽  
Electrochemical Microscopy

scholarly journals Reactive oxygen species formed in organic lithium–oxygen batteries

2016 ◽  
Vol 18 (16) ◽  
pp. 10774-10780 ◽  
Author(s):  
Patrick Schwager ◽  
Saustin Dongmo ◽  
Daniela Fenske ◽  
Gunther Wittstock
Keyword(s):  
Reactive Oxygen Species ◽  
Fluorescence Microscopy ◽  
Reactive Oxygen ◽  
Scanning Electrochemical Microscopy ◽  
Oxygen Species ◽  
Organic Electrolytes ◽  
Lithium Oxygen Batteries ◽  
Electrochemical Microscopy ◽  
Discharge Reaction

The generation of reactive oxygen species has been assumed to occur during the charging reaction of lithium-oxygen batteries with organic electrolytes. Here we show independently by fluorescence microscopy and scanning electrochemical microscopy that superoxide is also formed and released into the solution during the discharge reaction.

Hydrogen peroxide-responsive AIE probe for imaging-guided organelle targeting and photodynamic cancer cell ablation

2021 ◽  
Author(s):  
Qian Wu ◽  
Youmei Li ◽  
Ying Li ◽  
Dong Wang ◽  
Ben Zhong Tang
Keyword(s):  
Reactive Oxygen Species ◽  
Hydrogen Peroxide ◽  
Cancer Cell ◽  
Reactive Oxygen ◽  
Living Cells ◽  
Oxygen Species ◽  
Cell Ablation ◽  
Selective Monitoring ◽  
Organelle Targeting

Hydrogen peroxide (H2O2), as one kind of key reactive oxygen species (ROS), is mainly produced endogenously primarily in the mitochondria. The selective monitoring of H2O2 in living cells is of...

scholarly journals Intracellular Sources of ROS/H2O2 in Health and Neurodegeneration: Spotlight on Endoplasmic Reticulum

Cells ◽  
2021 ◽  
Vol 10 (2) ◽  
pp. 233
Author(s):  
Tasuku Konno ◽  
Eduardo Pinho Melo ◽  
Joseph E. Chambers ◽  
Edward Avezov
Keyword(s):  
Oxidative Stress ◽  
Reactive Oxygen Species ◽  
Hydrogen Peroxide ◽  
Endoplasmic Reticulum ◽  
Neurodegenerative Diseases ◽  
Antioxidative Enzymes ◽  
Redox Reactions ◽  
Ros Production ◽  
Oxygen Species ◽  
Specific Target

Reactive oxygen species (ROS) are produced continuously throughout the cell as products of various redox reactions. Yet these products function as important signal messengers, acting through oxidation of specific target factors. Whilst excess ROS production has the potential to induce oxidative stress, physiological roles of ROS are supported by a spatiotemporal equilibrium between ROS producers and scavengers such as antioxidative enzymes. In the endoplasmic reticulum (ER), hydrogen peroxide (H2O2), a non-radical ROS, is produced through the process of oxidative folding. Utilisation and dysregulation of H2O2, in particular that generated in the ER, affects not only cellular homeostasis but also the longevity of organisms. ROS dysregulation has been implicated in various pathologies including dementia and other neurodegenerative diseases, sanctioning a field of research that strives to better understand cell-intrinsic ROS production. Here we review the organelle-specific ROS-generating and consuming pathways, providing evidence that the ER is a major contributing source of potentially pathologic ROS.

scholarly journals Upconversion-based nanosystems for fluorescence sensing of pH and H2O2

2021 ◽  
Author(s):  
Chunning Sun ◽  
Michael Gradzielski
Keyword(s):  
Reactive Oxygen Species ◽  
Hydrogen Peroxide ◽  
Excitation Energy ◽  
Reactive Oxygen ◽  
Oxygen Species ◽  
Fluorescence Sensing ◽  
Living Organisms

Hydrogen peroxide (H2O2), a key reactive oxygen species, plays an important role in living organisms, industrial and environmental fields. Here, a non-contact upconversion nanosystem based on the excitation energy attenuation...

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