Development of enteropeptidase activity in mouse small intestine: influence of hormones

1985 ◽  
Vol 63 (5) ◽  
pp. 472-475 ◽  
Author(s):  
Pierre Arsenault ◽  
Daniel Ménard
Keyword(s):  
Small Intestine ◽  
Proximal Part ◽  
Major Effect ◽  
Enzymic Activity ◽  
Cortisone Acetate ◽  
Maximal Increase ◽  
Adult Level ◽  
Mouse Small Intestine ◽  
Epidermal Growth ◽  
Old Mice

The postnatal development of enteropeptidase activity has been examined on mucosal scrapping of the proximal part of the mouse small intestine. The activity was present at birth and remained low during the first 15 days of life. Then it rapidly increased reaching adult level within 2 days. Daily administration of cortisone acetate (25 μg∙g body weight (bw)−1∙day−1), insulin (12.5 mU∙g bw−1∙day−1), or epidermal growth factor (4 μg∙g bw−1∙day−1) during 3 days to 8-day-old mice induced a premature increase of enteropeptidase. The maximal increase was observed with cortisone treatment, the enzymic activity representing 70% of the adult level. Thyroxine alone (1 μg∙g bw−1∙day−1) had no significant effect on enteropeptidase activity. Hormonal interactions have been evaluated by studying the effects of different hormonal combinations. Finally, cortisone acetate which has a major effect on this activity during suckling period was unable to influence adult small intestinal enteropeptidase activity.

scholarly journals Gata4 Is Essential for the Maintenance of Jejunal-Ileal Identities in the Adult Mouse Small Intestine

2006 ◽  
Vol 26 (23) ◽  
pp. 9060-9070 ◽  
Author(s):  
Tjalling Bosse ◽  
Christina M. Piaseckyj ◽  
Ellen Burghard ◽  
John J. Fialkovich ◽  
Satish Rajagopal ◽  
...  
Keyword(s):  
Small Intestine ◽  
Cell Fate ◽  
Adult Mouse ◽  
Specific Model ◽  
Gene Encoding ◽  
Fatty Acid Binding ◽  
Adult Mice ◽  
Mouse Small Intestine ◽  
Old Mice

ABSTRACTGata4, a member of the zinc finger family of GATA transcription factors, is highly expressed in duodenum and jejunum but is nearly undetectable in distal ileum of adult mice. We show here that the caudal reduction of Gata4 is conserved in humans. To test the hypothesis that the regional expression of Gata4 is critical for the maintenance of jejunal-ileal homeostasis in the adult small intestine in vivo, we established an inducible, intestine-specific model that results in the synthesis of a transcriptionally inactiveGata4mutant. Synthesis of mutant Gata4 in jejuna of 6- to 8-week-old mice resulted in an attenuation of absorptive enterocyte genes normally expressed in jejunum but not in ileum, including those for the anticipated targets liver fatty acid binding protein (Fabp1) and lactase-phlorizin hydrolase (LPH), and a surprising induction of genes normally silent in jejunum but highly expressed in ileum, specifically those involved in bile acid transport. Inactivation ofGata4resulted in an increase in the goblet cell population and a redistribution of the enteroendocrine subpopulations, all toward an ileal phenotype. The gene encoding Math1, a known activator of the secretory cell fate, was induced ∼75% (P< 0.05). Gata4 is thus an important positional signal required for the maintenance of jejunal-ileal identities in the adult mouse small intestine.

scholarly journals In Vivo Imaging of Enteric Neurogenesis in the Deep Tissue of Mouse Small Intestine

PLoS ONE ◽  
2013 ◽  
Vol 8 (1) ◽  
pp. e54814 ◽  
Author(s):  
Kei Goto ◽  
Go Kato ◽  
Isao Kawahara ◽  
Yi Luo ◽  
Koji Obata ◽  
...  
Keyword(s):  
Small Intestine ◽  
In Vivo Imaging ◽  
Deep Tissue ◽  
Mouse Small Intestine

Is polyamine synthesis involved in the proliferative response of the intestinal epithelium to urogastrone-epidermal growth factor?

1989 ◽  
Vol 76 (6) ◽  
pp. 595-598 ◽  
Author(s):  
R. A. Goodlad ◽  
H. Gregory ◽  
N. A. Wright
Keyword(s):  
Cell Proliferation ◽  
Small Intestine ◽  
Growth Factor ◽  
Epidermal Growth Factor ◽  
Proliferative Response ◽  
Intestinal Cell ◽  
Intestinal Epithelial ◽  
Polyamine Synthesis ◽  
Proliferative Effect ◽  
Epidermal Growth

1. Intestinal epithelial cell proliferation was measured in rats maintained on total parenteral nutriton (TPN), in TPN rats given 300 μg of recombinant human epidermal growth factor (urogastrone-epidermal growth factor, URO-EGF) day−1 kg−1, and in further groups given URO-EGF and difluoromethylornithine (DFMO), an inhibitor of the enzyme ornithine decarboxylase (ODC). 2. URO-EGF significantly increased intestinal cell proliferation throughout the gastrointestinal tract. The proliferative response of the colon was particularly pronounced. 3. DFMO reduced the proliferative effect of urogastrone in the stomach and small intestine. DFMO also reduced URO-EGF-stimulated intestinal cell proliferation in the colon, but to a lesser extent. 4. It is concluded that ODC is essential for effecting the proliferative response of the stomach and small intestine to URO-EGF, but this role may be less important in the colon.

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