Genetic homogeneity based on skin histocompatibility and the evolution and systematics of parthenogenetic Cnemidophorus laredoensis (Sauria: Teiidae)

2000 ◽  
Vol 78 (6) ◽  
pp. 895-904
Author(s):  
Ramadan M Abuhteba ◽  
James M Walker ◽  
James E Cordes
Keyword(s):  
Parental Species ◽  
Skin Grafting ◽  
Genetic Homogeneity ◽  
Skin Grafts ◽  
Clonal Structure ◽  
Parthenogenetic Species ◽  
High Level

The parthenogenetic whiptail lizard Cnemidophorus laredoensis of Texas and México was derived from one or more hybrids between the gonochoristic species Cnemidophorus gularis and Cnemidophorus sexlineatus. We analyzed clonal structure in C. laredoensis on the basis of histocompatibility reactions to reciprocal skin grafting. Individuals of C. laredoensis rejected skin grafts from both parental species and from a hybrid between C. gularis and an undescribed parthenogenetic species. One lizard initially identified as C. laredoensis was found to be a triploid C. laredoensis × C. gularis hybrid, based on its histocompatibility reactions. The hybrid accepted grafts from individuals of C. laredoensis; however, C. laredoensis rejected grafts from this and a second hybrid. Seventeen individuals of C. laredoensis from six sites located throughout the range of the species in Texas were fully histocompatible, based on a large number of reciprocal skin grafts. Only three individuals of C. laredoensis from three sites donated grafts to other individuals of the species, but they were unable to host all or most of the reciprocal grafts. The skin histo compatibility among 17 individuals of C. laredoensis from six widely separated geographic sites points to a high level of genetic homogeneity in the species due to clonal descent from one parthenogenetic C. gularis × C. sexlineatus hybrid. Unidirectional rejection of skin grafts by three lizards could be a result of postformational mutations within this lineage, though this suggestion is open to question. The name C. laredoensis should be restricted to populations in Texas and México that are diagnostically identical with the parthenogenetic species described from two sites in Laredo, Webb County, Texas, in 1973.

668 Fixation of Split-Thickness Skin Grafts with Liquid Adhesive and Fibrin Glue in Place of Staples

2021 ◽  
Vol 42 (Supplement_1) ◽  
pp. S190-S191
Author(s):  
Joshua Frost ◽  
Nathan Hallier ◽  
Tanir Moreno ◽  
Jared Covell ◽  
Ryan Keck ◽  
...  
Keyword(s):  
Fibrin Glue ◽  
Skin Grafting ◽  
Skin Grafts ◽  
Inclusion Criteria ◽  
Intact Skin ◽  
Body Area ◽  
Wound Site ◽  
Graft Take ◽  
Thickness Skin ◽  
Split Thickness Skin Grafting

Abstract Introduction A critical component of split-thickness skin grafting is the fixation of the skin graft to the wound site. Graft displacement can result in graft failure, especially during the initial 48–72 hours following application. The most common method of securing grafts is with the use of staples, sometimes with the addition of fibrin glue in order to aid both graft adhesion and homeostasis. The use of staples, however, is associated with significant levels of patient discomfort, especially during staple removal. A possible alternative to staples is the use of liquid adhesives, in combination with steri-strips, to anchor the edges of skin grafts to intact skin. Certain liquid adhesives, such as gum-based resins, are cheaper to use than staples and offer the potential to secure small split-thickness skin grafts without the associated pain of staples. In this pilot study, we examined the effectiveness of using a combination of gum-based resin (Gum Mastic-Storax-Msal-Alcohol), fibrin glue, and steri-strips to secure partial-thickness grafts in 8 patients without the use of staples or sutures. Methods Patients were included in the study who required split-thickness skin grafts to treat wounds involving less than or equal to 15% total surface body area and whose wounds were not located in areas prone to graft displacement, such as the axilla and groin. For each patient, skin grafts were secured using fibrin glue (sprayed over the entire wound), and a combination of liquid adhesive and steri-strips applied around the wound perimeter. The success of each graft was determined by the percentage of graft take. Results From January 1st, 2020 to April 30th, 2020, 8 patients were identified who fit the inclusion criteria. Five of the patients received grafts to their lower extremities, two patients received grafts to their upper extremities, and one of the patients received a graft to the torso. The average wound site that was grafted was 116.7 cm2. Average graft take among the 8 patients was 96.9%, with a range of 90%-100%. No complications at the graft site were noted, such as hematomas or any other event that resulted in graft displacement or failure. Conclusions The results of the study demonstrate that a combination of liquid adhesive, fibrin glue, and steri-strips, can be used as an effective alternative to staples in small split-thickness skin grafts. The use of liquid adhesive in place of staples was advantageous because it eliminated to need for staple removal, which resulted in less discomfort for the patient and less work for the nursing staff.

scholarly journals A cross-sectional study of long-term satisfaction after surgery for congenital syndactyly: does skin grafting influence satisfaction?

2018 ◽  
Vol 44 (3) ◽  
pp. 296-303 ◽  
Author(s):  
Bien R. Ferrari ◽  
Paul M.N. Werker
Keyword(s):  
Donor Site ◽  
Skin Grafting ◽  
Cross Sectional Study ◽  
Complication Rates ◽  
Skin Grafts ◽  
Sectional Study ◽  
Level Of Evidence ◽  
Cross Sectional ◽  
Scar Assessment

Syndactyly correction without skin grafting is advocated because it prevents graft-related complications and donor site morbidity. In this cross-sectional study, we compared satisfaction among patients who underwent correction with and without skin grafting to determine preference based on subjective and objective parameters. Retrospective chart analysis was performed among 27 patients (49 webs) who were seen at follow-up after a median follow-up period of 7.4 years, at which the Patient and Observer Scar Assessment Scale, the Withey score and a satisfaction survey were used. Notably, there were no significant differences in complication rates or observer rated scar scores. Although the need for an additional surgical procedure was higher after skin grafting, patient-rated satisfaction scores were similar irrespective of the use of grafting. Our data suggest that corrections can best be performed without skin grafts if seeking to minimize the need for an additional procedure, but that the use of skin grafts does not appear to affect patient satisfaction. Level of evidence: IV

Expansion potential of skin grafts with novel I-shaped auxetic incisions

2021 ◽  
Author(s):  
Vivek Gupta ◽  
Arnab Chanda
Keyword(s):  
Skin Grafting ◽  
Burn Injuries ◽  
Skin Grafts ◽  
Severe Burn ◽  
Thickness Skin ◽  
Split Thickness Skin Grafting ◽  
Shape Pattern ◽  
Severe Burn Injuries ◽  
Poisson's Ratios ◽  
Poisson’S Ratios

Abstract Severe burn injures lead to millions of fatalities every year due to lack of skin replacements. While skin is a very limited and expensive entity, split thickness skin grafting, which involves the projection of a parallel incision pattern on a small section of healthy excised skin, is typically employed to increase the expansion and cover a larger burn site. To date, the real expansion capacity of such grafts are low (<3 times) and insufficient for treatment of severe burn injuries. In this study, novel I-shaped auxetic incision patterns, which are known to exhibit high negative Poisson’s ratios, have been tested on the skin to investigate their expansion potential. Fourteen two-layer skin graft models with varying incision pattern parameters (i.e., length, spacing, and orientation) were developed using finite element modelling and tested under uniaxial and biaxial tensile loads. The Poisson’s ratio, meshing ratios, and induced stresses were quantified across all models. Graft models tested uniaxially along the orthogonal directions indicated opposite trends in generated Poisson’s ratios, as the length of the I-shape incisions were increased. Biaxially, with a symmetric and closely spaced I-shape pattern, graft meshing ratios up to 15.65 were achieved without overstressing the skin. Overall, the findings from the study indicated that expansion potentials much higher than that of traditional skin grafts can be achieved with novel I-shaped auxetic skin grafts, which would be indispensable for covering large wounds in severe burn injuries.

Skin Grafting

2019 ◽  
pp. 65-72
Author(s):  
Stephen M. Milner
Keyword(s):  
Skin Grafting ◽  
Skin Grafts ◽  
Full Thickness ◽  
Preoperative Preparation ◽  
Scar Contracture ◽  
Skin Defects ◽  
Full Thickness Skin ◽  
Thickness Skin ◽  
Contracture Release

Skin grafting is an indispensable technique used in a variety of clinical situations, including acute burns, traumatic wounds, scar contracture release, and oncological and congenital deficiencies. The author’s preferred techniques for harvesting and resurfacing various skin defects using split- and full-thickness skin grafts are described in this chapter, together with the assessment of donor and recipient sites, preoperative preparation and postoperative considerations.

Migration and Hereditary Disease in the Saguenay Population of Eastern Quebéc

1987 ◽  
Vol 21 (3) ◽  
pp. 592-608 ◽  
Author(s):  
Margaret I. Gradie ◽  
Danielle Gauvreau
Keyword(s):  
Genetic Structure ◽  
Genetic Disease ◽  
Genetic Diseases ◽  
Large Population ◽  
Hereditary Disease ◽  
Genetic Homogeneity ◽  
Preliminary Results ◽  
Consanguineous Marriages ◽  
High Level ◽  
The Relationship

This article examines the relationship between migration and genetic disease in the situation of the Saguenay region of Quέbec. This large population shows an elevated incidence of several genetic diseases. The process of migration, which created the population, is thought to be a major factor in determining the genetic structure of the contemporary population. Preliminary results suggest that although consanguineous marriages are not and never were frequent in the population, socially, kinship was important in determining migration and persistence, leading to a high level of genetic homogeneity today.

Use of Mechanical Stretching to Treat Skin Graft Contracture

2020 ◽  
Vol 41 (4) ◽  
pp. 892-899
Author(s):  
Jinfeng Zhou ◽  
Youcai Zhao ◽  
Wengbo Yang ◽  
Qianming Du ◽  
Jun Yin ◽  
...  
Keyword(s):  
Skin Graft ◽  
Smooth Muscle Actin ◽  
Picric Acid ◽  
Skin Grafting ◽  
Control Group ◽  
Skin Grafts ◽  
Alpha Smooth Muscle Actin ◽  
Treatment Measures ◽  
And Function ◽  
Mechanical Stretching

Abstract After transplantation, skin grafts contract to different degrees, thus affecting the appearance and function of the skin graft sites. The exact mechanism of contracture after skin grafting remains unclear, and reliable treatment measures are lacking; therefore, new treatment methods must be identified. Many types of centripetal contraction forces affect skin graft operation, thus leading to centripetal contracture. Therefore, antagonizing the centripetal contraction of skin grafts may be a feasible method to intervene in skin contracture. Here, the authors propose the first reported mechanical stretching method to address contracture after skin grafting. A full-thickness skin graft model was established on the backs of SD rats. The skin in the experimental group was stretched unilaterally or bidirectionally with a self-made elastic stretching device, whereas the skin was non-stretched in the control group. The rats were sacrificed 2 weeks after stretching. The area, length, and width of the skin were measured. The grafts were cut and fixed with formalin. Routine paraffin sections were stained with hematoxylin-eosin, picric acid-Sirius red, Victoria blue, and anti-alpha-smooth muscle actin (SMA). Mechanical stretching made the graft lengthen in the direction of the stress and had an important influence on collagen deposition and alpha-SMA expression in the graft. This method warrants further in-depth study to provide a basis for clinical application.

CORRECTIVE SURGERY OF SYNDACTYLY WITHOUT THE USE OF SKIN GRAFTS

Hand Surgery ◽  
1996 ◽  
Vol 01 (02) ◽  
pp. 203-209 ◽  
Author(s):  
D. Vickers ◽  
W. Donnelly
Keyword(s):  
Operative Technique ◽  
Skin Grafting ◽  
Skin Grafts ◽  
Corrective Surgery ◽  
Simple Complex ◽  
Good Outcome

This paper presents an operative technique of syndactyly release (syndactylysis) without skin grafting. A series of 31 cases, of simple, complex and complicated syndactylies are presented with a mean follow-up of 4.4 years. The results demonstrate that a good outcome can be reliably obtained by syndactylysis without the use of skin grafts.

The Crucial Role of Vascularization and Lymphangiogenesis in Skin Reconstruction

2018 ◽  
Vol 59 (3-4) ◽  
pp. 242-254 ◽  
Author(s):  
Florian S.  Frueh ◽  
Nadia Sanchez-Macedo ◽  
Maurizio Calcagni ◽  
Pietro Giovanoli ◽  
Nicole Lindenblatt
Keyword(s):  
Clinical Practice ◽  
Skin Grafting ◽  
Lymphatic Drainage ◽  
Skin Grafts ◽  
Skin Substitutes ◽  
Microvascular Network ◽  
Skin Defects ◽  
Dermal Substitutes ◽  
Skin Reconstruction

Background: The treatment of extensive skin defects and bradytrophic wounds remains a challenge in clinical practice. Despite emerging tissue engineering approaches, skin grafts and dermal substitutes are still the routine procedure for the majority of skin defects. Here, we review the role of vascularization and lymphangiogenesis for skin grafting and dermal substitutes from the clinician’s perspective. Summary: Graft revascularization is a dynamic combination of inosculation, angiogenesis, and vasculogenesis. The majority of a graft’s microvasculature regresses and is replaced by ingrowing microvessels from the wound bed, finally resulting in a chimeric microvascular network. After inosculation within 48–72 h, the graft is re-oxygenated. In contrast to skin grafts, the vascularization of dermal substitutes is slow and dependent on the ingrowth of vessel-forming angiogenic cells. Preclinical angiogenic strategies with adipose tissue-derived isolates are appealing for the treatment of difficult wounds and may markedly accelerate skin reconstruction in the future. However, their translation from bench to bedside is still restricted by major regulatory restrictions. Finally, the lymphatic system contributes to edema reduction and the removal of local wound debris. Therapeutic lymphangiogenesis is an emerging field of research in skin reconstruction. Key Messages: For the successful engraftment of skin grafts and dermal substitutes, the rapid formation of a microvascular network is of pivotal importance. Hence, to understand the biological processes behind revascularization of skin substitutes and to implement this knowledge into clinical practice is a prerequisite when treating skin defects. Furthermore, a functional lymphatic drainage crucially contributes to the engraftment of skin substitutes.

scholarly journals Cutaneous vascular and sudomotor responses in human skin grafts

2010 ◽  
Vol 109 (5) ◽  
pp. 1524-1530 ◽  
Author(s):  
Craig G. Crandall ◽  
Scott L. Davis
Keyword(s):  
Heat Stress ◽  
Heat Dissipation ◽  
Skin Grafting ◽  
Whole Body ◽  
Sweat Glands ◽  
Skin Grafts ◽  
Local Cooling ◽  
Doppler Flowmetry ◽  
Graft Area ◽  
The Individual

Each year millions of individuals sustain burns. Within the US 40,000–70,000 individuals are hospitalized for burn-related injuries, some of which are quite severe, requiring skin grafting. The grafting procedure disrupts neural and vascular connections between the host site and the graft, both of which are necessary for that region of skin to contribute to temperature regulation. With the use of relatively modern techniques such as laser-Doppler flowmetry and intradermal microdialysis, a wealth of information has become available regarding the consequences of skin grafting on heat dissipation and heat conservation mechanisms. The prevailing data suggest that cutaneous vasodilator capacity to an indirect heat stress (i.e., heating the individual but not the evaluated graft area) and a local heating stimulus (i.e., directly heating the graft area) is impaired in grafted skin. These impairments persist for ≥4 yr following the grafting procedures and are perhaps permanent. The capacity for grafted skin to vasodilate to an endothelial-dependent vasodilator is likewise impaired, whereas its capacity to vasodilate to an endothelial-independent vasodilator is generally preserved. Sweating responsiveness is minimal to nonexistent in grafted skin to both a whole body heat stress and local administration of the primary neurotransmitter responsible for stimulating sweat glands (i.e., acetylcholine). Likewise, there is no evidence that this absence of sweat gland responsiveness improves as the graft matures. In contrast to the heating stimuli, cutaneous vasoconstrictor responses to both indirect whole body cooling (i.e., exposing the individual to a cold stress but not at the evaluated graft area) and direct local cooling (i.e., directly cooling the graft area) are preserved in grafted skin as early as 5–9 mo postgrafting. If uninjured skin does not compensate for impaired heat dissipation of grafted skin, individuals having skin grafts encompassing significant fractions of their body surface area will be at a greater risk for a hyperthermic-related injury. Conversely, the prevailing data suggest that such individuals will not be at a greater risk of hypothermia upon exposure to cold environmental conditions.

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