Development of the giant kidney worm, Dioctophyma renale (Goeze, 1782) (Nematoda: Dioctophymatoidea)

1975 ◽  
Vol 53 (11) ◽  
pp. 1552-1568 ◽  
Author(s):  
T. F. Mace ◽  
R. C. Anderson
Keyword(s):  
Blood Vessel ◽  
Rana Catesbeiana ◽  
Abdominal Muscles ◽  
Lumbriculus Variegatus ◽  
Larval Stages ◽  
Infective Larvae ◽  
Ictalurus Nebulosus ◽  
Third Stage ◽  
Paratenic Hosts ◽  
The Right

Dioctophyma renale was found in the right kidney of 48% of mink (Mustela vison) trapped in late fall and winter in the Black River area of Ontario. Eggs embryonated at temperatures from 14 C to 30 C. Eggs hatched in the intestine of the aquatic oligochaete Lumbriculus variegatus, and first-stage larvae migrated to the ventral blood vessel where development took place. The first molt occurred about 50 days after infection in oligochaetes kept at 20 C; the second molt occurred about 100 days after infection. Third-stage larvae removed from oligochaetes produced infection in a mink. Larvae given to frogs (i.e. Rana clamitans, melanota and R. pipiens) became encapsulated in the stomach wall or abdominal muscles. A mink was infected with larvae removed from frogs experimentally infected 25 days earlier. A mink was also infected with larvae found in a naturally infected bullhead (Ictalurus nebulosus). Infective larvae of D. renale were found in wild Rana catesbeiana (6.2%), R. septentrionalis (9.6%), and R. clamitans melanota (0.7%) in an area enzootic for dioctophymiasis. It is suggested frogs as well as bullheads are important natural paratenic hosts for D. renale. The various larval stages of D. renale are described and its third-stage larva is distinguished from that of Eustrongylides spp. which may also be found in frogs.

MORPHOLOGICAL AND BIOLOGICAL DIFFERENCES BETWEEN THE INFECTIVE LARVAE OF ANGIOSTRONGYLUS CANTONENSIS AND THOSE OF ANAFILAROIDES ROSTRATUS

1963 ◽  
Vol 41 (7) ◽  
pp. 1179-1183 ◽  
Author(s):  
Joseph E. Alicata
Keyword(s):  
The Body ◽  
Angiostrongylus Cantonensis ◽  
Infective Larvae ◽  
Anal Opening ◽  
Third Stage ◽  
Rat Lungworm ◽  
The Central Nervous System ◽  
Paratenic Hosts ◽  
Rats And Mice ◽  
Biological Differences

Third-stage larvae of the rat lungworm, Angiostrongylus cantonensis, can be differentiated from those of the cat lungworm, Anafilaroides rostratus, on the basis of shorter body length, shorter esophagus, and in some cases on the position of the genital primordium. In A. cantonensis the length of the body is 460 to 510 μ and the length of the esophagus, including the buccal cavity, is 171 to 198 μ. The genital primordium is 120 to 135 μ from the anal opening. In A. rostratus the length of the body is 545 to 655 μ, and that of the esophagus is 218 to 246 μ. The genital primordium is 37 to 153 μ from the anal opening. The infective larvae of A. rostratus do not migrate to the central nervous system as do those of A. cantonensis. The larvae of A. rostratus have been found to re-encyst in the liver of rats and mice which serve as paratenic hosts.

Studies on resistance in calves to experimental infection with the nodular worm, Oesophagostomum radiatum (Rudolphi, 1803). III. The stimulation of resistance by multiple infections with larval stages

1967 ◽  
Vol 18 (4) ◽  
pp. 707 ◽  
Author(s):  
RK Keith
Keyword(s):  
Test Dose ◽  
Multiple Infections ◽  
Pathological Changes ◽  
Fourth Stage ◽  
Larval Stages ◽  
Infective Larvae ◽  
Lower Weight ◽  
Third Stage ◽  
High Degree ◽  
Stimulation Of

Observations are reported on the influence of multiple infections with larval stages of Oesophagostomum radiatum on the stimulation of resistance in calves to reinfection with this parasite. Calves were given spaced doses of infective larvae, and the resulting infections were terminated in the early fourth stage by treatment with an anthelmintic. The egg counts, numbers of adult worms recovered at necropsy, and the pathological changes observed in the intestine subsequent to a test dose indicated that the reinfected calves had developed a high degree of resistance. The reinfected calves made significantly lower weight gains than uninfected control calves during the period of exposure to the larval infections. The results suggest that the immunogenic products of adult worms, and of third stage or early fourth stage larvae, are qualitatively similar.

ON THE DEVELOPMENT, MORPHOLOGY, AND EXPERIMENTAL TRANSMISSION OF DIPLOTRIAENA BARGUSINICA (FILARIOIDEA : DIPLOTRIAENIDAE)

1962 ◽  
Vol 40 (7) ◽  
pp. 1175-1186 ◽  
Author(s):  
Roy C. Anderson
Keyword(s):  
Definitive Host ◽  
Fat Body ◽  
The Other ◽  
Infective Stage ◽  
Fourth Stage ◽  
Turdus Migratorius ◽  
Experimental Transmission ◽  
Larval Stages ◽  
Infective Larvae ◽  
Third Stage

Eggs of Diplotriaena bargusinica from worms found in a Hylocichla fuscescens from Algonquin Park, Ontario, hatched and developed in the fat body of grasshoppers (Melanoplus bilituratus, M. fasciatus, Camnula pellucida). At approximately 30–33 °C the first molt took place on the 9–11th day, the second on the 14–16th day. Most second- and third-stage larvae were enveloped in capsules, more than one larva often being present in a single capsule. The larval stages from grasshoppers are described. Grasshoppers with infective larvae were fed to two H. fuscescens, one H. ustulata. one Turdus migratorius, and one Quiscalus quiscula. Subadult worms were found in the two H. fuscescens and the H. ustulata 55–301 days later but none was found in the other birds examined 78–97 days later. Late fourth-stage larvae from the heart and aorta of a nestling H. ustulata captured in the field are described. It is believed tridents first make their appearance during development in the definitive host from the infective stage to the fourth stage. Observations on the morphology of adult D. bargusinica are also reported.

Larval and juvenile development of the Iceland cockle Ciliatocardium ciliatum (Fabricius, 1780) (Bivalvia: Cardiidae)

2020 ◽  
Vol 324 (2) ◽  
pp. 242-251
Author(s):  
L.P. Flyachinskaya ◽  
P.A. Lezin
Keyword(s):  
Lateral Position ◽  
Larval Shell ◽  
Subtidal Zone ◽  
Larval Stages ◽  
Posterior Shoulder ◽  
Blade Shape ◽  
The Right ◽  
Lateral Structures ◽  
Juvenile Development ◽  
Concentric Structure

The paper considers the development of Ciliatocardium ciliatum from the stage of straight hinge to juvenile. In the White Sea the spawning of C. ciliatum begins at the end of June, larvae at different stages of development occur in plankton until the end of September. The earliest of the larvae found had shell lengths of 123–130 µm. The paper first examined the anatomy and structure of the larval shell of C. ciliatum. During the development, the main stages of organogenesis were described and special attention was paid to the formation of the digestive and muscular systems. The digestive system begins to function when the larva reaches a size of 170–180 µm. The digestive gland has a two-blade shape and is shifted to the right side. The foot is formed at a size of 230 µm, the gill rudiments appear when the larva reaches 270 µm. The development of the larval shell and larval hinge of the mollusc is considered in detail. The development of the larval shell of C. ciliatum is similar to the development of other family members. Throughout all the larval stages, the shell has a rounded shape with a low umbos, and the prodissoconch II has a clearly visible concentric structure. The C. ciliatum larval hinge is characterized by weak differentiation and the absence of pronounced cardinal teeth typical for other Cardiidae. However, the lateral structures of the castle – ridges and flanges – are well developed. The ligament begins to form at a size of 240–250 µm and occupies a lateral position. The settlement of the cockle takes place in September in the subtidal zone. After the metamorphosis, a large radial sculpture is formed on the dissoconch and a number of small spikes are formed at the rib of the posterior shoulder.

Studies on the life-history of Bunostomum phlebotomum (Railliet, 1900), a hookworm parasite of cattle

Parasitology ◽  
1946 ◽  
Vol 37 (3-4) ◽  
pp. 192-201 ◽  
Author(s):  
J. F. A. Sprent
Keyword(s):  
Infective Larva ◽  
Stage Larva ◽  
Fourth Stage ◽  
Larval Stages ◽  
The Third ◽  
Third Stage ◽  
History Of ◽  
Time Required ◽  
Cutaneous Blood ◽  
Fourth Stage Larva

A description is given of the processes of copulation, formation of the egg and spermatozoon, cleavage, embryogeny and hatching in B. phlebotomum. These processes were found to be essentially similar to those in other strongyle nematodes.The anatomy of the first three larval stages is described and the observations of Conradi & Barnette (1908) and Schwartz (1924) were largely confirmed.Penetration of the skin of calves by the infective larva was observed histologically. The larvae were found to have reached the dermis within 30 min. and to have penetrated the cutaneous blood vessels within 60 min. of application to the skin. The larvae were found in the lung where the third ecdysis was in progress 10 days after penetration of the skin. A description is given of the growth of the third-stage larva in the lung, the changes which take place during the third ecdysis, and the anatomy of the fourth-stage larva.The fourth-stage larvae exsheath in the lungs and travel to the intestine. After a period of growth in which sexual differentiation takes place, the fourth ecdysis occurs and the adult parasite emerges. The time required for the attainment of maturity was found to be somewhere between 30 and 56 days after penetration of the skin.This paper was written at the Ministry of Agriculture and Fisheries Veterinary Laboratories, Wey-bridge, and the writer would like to express his gratitude to the Director, Prof. T. Dalling, also to Dr W. R. Wooldridge, chairman of the Council of the Veterinary Educational Trust for their help and encouragement. The writer's thanks are also due to Dr H. A. Baylis, Prof. R. T. Leiper and Dr E. L. Taylor for their advice and help on technical points, and to Mr R. A. O. Shonekan, African laboratory assistant, for his able co-operation.

scholarly journals Seasonal occurrence of some larval stages of endoparasites in three cyprinids from the Nwanedi-Luphephe dams, the Limpopo River System, South Africa

2015 ◽  
Vol 52 (3) ◽  
pp. 229-235 ◽  
Author(s):  
E. M. Mbokane ◽  
J. Theron ◽  
W. J. Luus-Powell
Keyword(s):  
Developmental Stages ◽  
River System ◽  
Intestinal Wall ◽  
The Body ◽  
Seasonal Occurrence ◽  
Seasonal Fluctuations ◽  
Metazoan Parasites ◽  
Larval Stages ◽  
Parasite Communities ◽  
Third Stage

Abstract This study provides information on seasonal occurrence of developmental stages of endoparasites infecting three cyprinids in the Nwanedi-Luphephe dams, Limpopo River System. Labeobarbus marequensis (Smith, 1841), Barbus trimaculatus Peters, 1852 and Barbus radiatus Peters, 1853 were investigated seasonally from January 2008 to October 2008. The following larvae of metazoan parasites were collected: Diplostomum sp. from the eyes of L. marequensis and B. trimaculatus; Ornithodiplostomum sp. from the gills of B. trimaculatus; Posthodiplostomum sp. from muscle, skin and fins of B. trimaculatus and B. radiatus; third-stage Contracaecum larvae (L3) from the mesentery fats and on the liver lobes of L. marequensis and B. trimaculatus and gryporynchid cestode larvae from the outer intestinal wall of B. radiatus. All the flukes encountered were metacercariae. Diplostomum sp. and Contracaecum sp. dominated the parasite communities. Their prevalence exhibited seasonal fluctuations with maxima in summer. Factors likely to influence fish infection such as the body size of fish and their condition factors were also briefly considered in this study.

The Physiology and Morphology of Median Nerve Motor Neurones in the Thoracic Ganglia of the Locust

1982 ◽  
Vol 96 (1) ◽  
pp. 325-341
Author(s):  
MALCOLM BURROWS
Keyword(s):  
Synaptic Input ◽  
Motor Neurone ◽  
Abdominal Muscles ◽  
Thoracic Ganglia ◽  
Synaptic Potentials ◽  
Metathoracic Ganglion ◽  
Inspiratory Motor ◽  
Motor Neurones ◽  
Chemical Synapses ◽  
The Right

Simultaneous intracellular recordings have been made from the two expiratory, and from the two inspiratory motor neurones which have their axons in the unpaired median nerves of the thoracic ganglia. Each motor neurone has an axon that branches to innervate muscles on the left and on the right side of one segment. The expiratory neurones studied were those in the meso- and meta-thoracic ganglia which innervate spiracular closer muscles. The depolarizing synaptic potentials underlying the spikes during expiration are common to the two closer motor neurones in a particular segment. Similarly, during inspiration when there are usually no spikes, the hyperpolarizing, inhibitory potentials are also common to both motor neurones. The synaptic input to the neurones can be derived from four interneurones; two responsible for the depolarizing potentials during expiration and two for the inhibitory potentials during inspiration. The inspiratory neurones studied were those in the abdominal ganglia fused to the metathoracic ganglion which innervate dorso-ventral abdominal muscles. During inspiration the two motor neurones of one segment spike at a similar and steady frequency. The underlying synaptic input to the two is common. During expiration, when there are usually no spikes, the hyperpolarizing synaptic potentials are also common to both neurones. In addition they match exactly the depolarizing potentials occurring at the same time in the closer motor neurones. The same set of interneurones could be responsible. No evidence has been revealed to indicate that the two closer, or the two inspiratory motor neurones of one segment are directly coupled by electrical or chemical synapses. The morphology of both types of motor neurone is distinct from that of other motor neurones in these ganglia. Both types branch extensively in both the left and in the right areas of the neuropile.

Improved Methods of Obtaining Clean Larvae of Haemonchus contortus

1963 ◽  
Vol 37 (4) ◽  
pp. 251-254 ◽  
Author(s):  
M. G. Christie ◽  
J. E. Patterson
Keyword(s):  
Free Surface ◽  
Surface Area ◽  
Haemonchus Contortus ◽  
Water Repellent ◽  
Optimum Conditions ◽  
Infective Larvae ◽  
Mucous Coat ◽  
Third Stage ◽  
Free Surface Area ◽  
Improved Methods

A pellet of sheep faeces containing eggs of Haemonchus contortus forms an adequate environment for the development of those eggs to third stage infective larvae, provided that it is kept moist. Observation shows a concentration of developing larvae on the external mucous coat of the pellet. These data suggest that optimum conditions for development would occur when pellets are separated one from another and standing on a water repellent surface, thus presenting the maximum free surface area and minimum opportunity for aggregation and hence of over crowding. The best method of recovering the infective larvae would be one that obtained larvae free from contamination without requiring them to expend energy in separating themselves from the contamination.

Studies on resistance of sheep to infestation with Haemonchus contortus and Trichostrongylus spp. and on the immunological reactions of sheep exposed to infestation. I. The preparation of antigens for the complement fixation test and the reactivity of the biochemical fractions of H. contortus

1950 ◽  
Vol 1 (3) ◽  
pp. 285 ◽  
Author(s):  
DF Stewart
Keyword(s):  
Haemonchus Contortus ◽  
Complement Fixation Test ◽  
Complement Fixation ◽  
Fixation Test ◽  
Mature Adult ◽  
Infective Larvae ◽  
Lipid Fractions ◽  
Significant Difference ◽  
Third Stage ◽  
Free Material

All antigen was developed to detect circulating antibodies by means of the complement fixation test in sheep infested with Haemonchus contortus and Trichostrongylus spp. Extraction of worm material at 100°C. for 10 minutes was found to be the most satisfactory method for the preparation of antigens. Potent antigens were prepared from young adult H. contortus, from third-stage infective larvae, and from the eggs. Old mature adult H. contortus yielded antigens of low potency. No significant difference was found between the potency of antigens prepared from male and female adult H. contortus collected from the same sheep. Both adult Trichostrongylus spp. and third-stage infective larvae consistently yielded antigens of high potency. No significant difference was found in the results obtained with larval or adult H. contortus antigens, adjusted to the same potency and tested with natural H. contortus antisera. H. contortus and Trichostrongylus spp. absorbed the antiserum to each other. It was shown that a lipid was an essential constituent of the boiled antigen in the complement fixation reaction with natural antisera. Lipid-free antigens from H. contortus failed to react with natural antisera. The lipid was not antigenic when injected into rabbits. The carbohydrate fraction of H. contortus did not fix complement in the presence of natural antisera and was not antigenic when injected into rabbits. The lipid fraction of a variety of nematode parasites reacted with natural antisera to H. contortus infestation. Similar lipid fractions of two species of trematodes did not react with natural antisera to H. contortus infestation. Normal saline suspensions of the lipid-free material from a variety of helminths showed a greater degree of specificity when tested with artificially prepared antisera than did the lipid fractions.

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