Ciliation and function of the food-collecting and waste-rejecting organs of lophophorates

1978 ◽  
Vol 56 (10) ◽  
pp. 2142-2155 ◽  
Author(s):  
T. H. J. Gilmour
Keyword(s):  
Electron Microscopy ◽  
Transmission Electron Microscopy ◽  
Waste Material ◽  
Suspension Feeding ◽  
Food Material ◽  
Water Currents ◽  
Excess Water ◽  
Transmission Electron ◽  
Food Particles ◽  
And Function

Experiments with mixtures of colloidal graphite, stained Sephadex particles, and algae; observations by stroboscopic interference contrast optics; and scanning and transmission electron microscopy suggest that phoronids, brachiopods, and bryozoans can simultaneously reject waste material by an impingement mechanism and accept edible particles by a filtration mechanism without the ciliary reversals suggested in previous models of suspension feeding in lophophorates. Specialized laterofrontal cilia, which may detect heavy inedible particles, are found on the tentacles of all three phyla of lophophorates. In phoronids and bryozoans edible material is carried towards the esophagus by components of water currents created by the lateral cilia of the tentacles of the lophophore while inedible particles are rejected by the frontal cilia of the tentacles. The passage of food material to the mouths of brachiopods is assisted by frontal cilia located in grooves on alternate tentacles while the frontal cilia of ungrooved tentacles reject inedible material. The epistomes of lophophorates are also involved in the simultaneous acceptance of food and rejection of solid waste material and allow the escape of excess water travelling towards the mouth with food particles. This finding of a functional significance for the epistome suggests that lophophorates deserve reassessment as possible ancestors of chordates.

The structure, ciliation, and function of the lips of some bivalve molluscs

1974 ◽  
Vol 52 (3) ◽  
pp. 335-343 ◽  
Author(s):  
T. H. J. Gilmour
Keyword(s):  
Suspension Feeding ◽  
Bivalve Molluscs ◽  
Food Material ◽  
Water Currents ◽  
Excess Water ◽  
And Function

The structure, ciliation, and function of the lips of 12 species belonging to nine families of the subclass Pteriomorphia are described. All these species have devices for preventing the swallowing of excess water, collected along with participate food material, by the ctenidia and palps. It is suggested that in all bivalves, as in other suspension-feeding animals, water currents are generated by the food-collecting apparatus. But, whereas in members of the echinoderm superphylum, the development of gill slits to allow the escape of excess water which has already entered the mouth has had great evolutionary implications, the escape of water before it gets to the mouth is usually achieved in a less conspicuous manner in bivalves, although a complex lip apparatus has been developed in some monomyarian members of the Pteriomorphia.

scholarly journals Quercetin ameliorates mitochondrial dysfunction and mitigates methamphetamine-induced anxiety-like behavior

2021 ◽  
Author(s):  
Fengrong Chen ◽  
Jiaxue Sun ◽  
Yongjin Zhang ◽  
Yicong Dai ◽  
Zherui Zhang ◽  
...  
Keyword(s):  
Electron Microscopy ◽  
Transmission Electron Microscopy ◽  
Oxygen Consumption ◽  
Mitochondrial Dysfunction ◽  
Consumption Rate ◽  
Mitochondrial Morphology ◽  
Transmission Electron ◽  
Anxiety Behavior ◽  
And Function

Methamphetamine (MA) abuse results in neurotoxic outcomes, including increased anxiety and depression, during both MA use and withdrawal. Although numerous studies have reported an association between MA exposure and anxiety, the underlying mechanism remains elusive. In this study, escalating dose of MA was used to establish an MA-treated mouse model presenting anxiety behavior. RNA seq was then performed to profile the gene expression patterns in the hippocampus (HIPP). Differentially expressed genes (DEGs) were identified and function enrichment analysis was conducted to explore the underlying mechanisms. Quercetin as an mitochondria protector was used in vivo and in vitro. The C57BL/6J mice were co-treated with 50 mg/kg Quercetin and escalating MA. Anxiety behavior was evaluated by utilizing the elevated plus maze and the open field test. Transmission electron microscopy and immunohistochemistry were conducted to study the pathology of MA-inducced anxiety . The effects of MA and Quercetin on astrocytes were investigated by fluorescence staining, transmission electron microscopy, flow cytometry, and oxygen consumption rate. Western blot and qPCR were performed to analyze altered protein and gene levels of HIPP in mice and astrtocytes. The results demonstrated that forteen upregulated differentially expressed genes were identified and significantly enriched in signaling pathways related to psychiatric disorders and mitochondrial function. Interestingly, we found that quercetin was able to alleviate MA-induced anxiety-like behavior by improving neuron number and mitochondria injury. Mechanistically, quercetin can mitigate aberrant mitochondrial morphology and mitochondrial dysfunction not only by decreasing the levels of total cytoplasmic reactive oxygen species (ROS), mitochondria-derived ROS (mtROS), and mitochondrial membrane potential (MMP), but also increasing the oxygen consumption rate (OCR) and mitochondrial ATP production in vitro, indicating Quercetin ameliorated MA-induced anxiety-like behavior by modifying mitochondrial morphology and function. Furthermore, quercetin reversed OPA1 and DRP1 expression in astrocytes, and mitigated astrocyte activation and the release of inflammatory factors, which can trigger neuronal apoptosis and synaptic loss. Taken together, we provided evidence showing that MA can induce anxiety-like behavior via the induction of oxidative stress and mitochondrial dysfunction. Quercetin exerted antipsychotic activity through mitochondrial modulation, suggesting its potential for further therapeutic development in MA-induced anxiety.

Progress in environmental high-voltage transmission electron microscopy for nanomaterials

2020 ◽  
Vol 378 (2186) ◽  
pp. 20190602
Author(s):  
Nobuo Tanaka ◽  
Takeshi Fujita ◽  
Yoshimasa Takahashi ◽  
Jun Yamasaki ◽  
Kazuyoshi Murata ◽  
...  
Keyword(s):  
Electron Microscopy ◽  
Transmission Electron Microscopy ◽  
High Voltage ◽  
High Energy ◽  
Transmission Electron ◽  
Recent Developments ◽  
In Situ Observations ◽  
High Voltage Transmission ◽  
And Function

A new environmental high-voltage transmission electron microscope (E-HVEM) was developed by Nagoya University in collaboration with JEOL Ltd. An open-type environmental cell was employed to enable in-situ observations of chemical reactions on catalyst particles as well as mechanical deformation in gaseous conditions. One of the reasons for success was the application of high-voltage transmission electron microscopy to environmental (in-situ) observations in the gas atmosphere because of high transmission of electrons through gas layers and thick samples. Knock-on damages to samples by high-energy electrons were carefully considered. In this paper, we describe the detailed design of the E-HVEM, recent developments and various applications. This article is part of a discussion meeting issue ‘Dynamic in situ microscopy relating structure and function'.

scholarly journals Comparative ultrastructure of the radiolar crown in Sabellida (Annelida)

Zoomorphology ◽  
2020 ◽  
Author(s):  
Ekin Tilic ◽  
Greg W. Rouse ◽  
Thomas Bartolomaeus
Keyword(s):  
Electron Microscopy ◽  
Transmission Electron Microscopy ◽  
Characteristic Feature ◽  
Water Currents ◽  
Internal Stabilization ◽  
Transmission Electron ◽  
Phylogenetic Lineages ◽  
Histology And Immunohistochemistry ◽  
Comparative Ultrastructure

AbstractThree major clades of tube-dwelling annelids are grouped within Sabellida: Fabriciidae, Serpulidae and Sabellidae. The most characteristic feature of these animals is the often spectacularly colorful and flower-like radiolar crown. Holding up such delicate, feathery appendages in water currents requires some sort of internal stabilization. Each of the above-mentioned family-ranked groups has overcome this problem in a different way. Herein we describe the arrangement, composition and ultrastructure of radiolar tissues for fabriciids, sabellids and serpulids using transmission electron microscopy, histology and immunohistochemistry. Our sampling of 12 species spans most of the phylogenetic lineages across Sabellida and, from within Sabellidae, includes representatives of Myxicolinae, Sabellinae and the enigmatic sabellin Caobangia. We further characterize the ultrastructure of the chordoid cells that make up the supporting cellular axis in Sabellidae and discuss the evolution of radiolar tissues within Sabellida in light of the recently published phylogeny of the group.

scholarly journals Analysis of complex, beam-sensitive materials by transmission electron microscopy and associated techniques

2020 ◽  
Vol 378 (2186) ◽  
pp. 20190601
Author(s):  
Martha Ilett ◽  
Mark S'ari ◽  
Helen Freeman ◽  
Zabeada Aslam ◽  
Natalia Koniuch ◽  
...  
Keyword(s):  
Electron Microscopy ◽  
Transmission Electron Microscopy ◽  
Electron Beam ◽  
Molecular Crystals ◽  
Optimum Dose ◽  
Tem Analysis ◽  
Transmission Electron ◽  
Imaging And Spectroscopy ◽  
And Function

We review the use of transmission electron microscopy (TEM) and associated techniques for the analysis of beam-sensitive materials and complex, multiphase systems in-situ or close to their native state. We focus on materials prone to damage by radiolysis and explain that this process cannot be eliminated or switched off, requiring TEM analysis to be done within a dose budget to achieve an optimum dose-limited resolution. We highlight the importance of determining the damage sensitivity of a particular system in terms of characteristic changes that occur on irradiation under both an electron fluence and flux by presenting results from a series of molecular crystals. We discuss the choice of electron beam accelerating voltage and detectors for optimizing resolution and outline the different strategies employed for low-dose microscopy in relation to the damage processes in operation. In particular, we discuss the use of scanning TEM (STEM) techniques for maximizing information content from high-resolution imaging and spectroscopy of minerals and molecular crystals. We suggest how this understanding can then be carried forward for in-situ analysis of samples interacting with liquids and gases, provided any electron beam-induced alteration of a specimen is controlled or used to drive a chosen reaction. Finally, we demonstrate that cryo-TEM of nanoparticle samples snap-frozen in vitreous ice can play a significant role in benchmarking dynamic processes at higher resolution. This article is part of a discussion meeting issue ‘Dynamic in situ microscopy relating structure and function’.

scholarly journals Immuno- and affinity probes for electron microscopy: a review of labeling and preparation techniques.

1990 ◽  
Vol 38 (2) ◽  
pp. 145-157 ◽  
Author(s):  
J W Stirling
Keyword(s):  
Electron Microscopy ◽  
Transmission Electron Microscopy ◽  
Critical Analysis ◽  
Cell Structure ◽  
Structure And Function ◽  
Sectioned Material ◽  
Transmission Electron ◽  
And Function ◽  
Affinity Probes ◽  
Preparation Techniques

Immuno- and affinity probes are widely used in biology and medicine, and are becoming essential tools for the elucidation of cell structure and function. This article reviews and discusses the bewildering array of probes and preparation techniques now available for the investigation of sectioned material by transmission electron microscopy, with critical analysis of their merits. Emphasis is placed on immunogold probes and methods useful for routine preparation, gathering together information that may be used to improve labeling techniques. New data on inert dehydration for the localization of sensitive epitopes without chemical or cryofixation is presented.

Techniques for Transmission Electron Microscopy of Fiber-Matrix Interfaces in Aluminum Alloy-Boron Composites by Ion Erosio

1971 ◽  
Vol 29 ◽  
pp. 204-205
Author(s):  
G. G. Shaw
Keyword(s):  
Electron Microscopy ◽  
Transmission Electron Microscopy ◽  
Aluminum Alloy ◽  
Electron Beam ◽  
Pure Aluminum ◽  
Ion Milling ◽  
Transmission Electron ◽  
Fiber Matrix ◽  
Ion Erosion ◽  
Row Of Fibers

The morphology and composition of the fiber-matrix interface can best be studied by transmission electron microscopy and electron diffraction. For some composites satisfactory samples can be prepared by electropolishing. For others such as aluminum alloy-boron composites ion erosion is necessary.When one wishes to examine a specimen with the electron beam perpendicular to the fiber, preparation is as follows: A 1/8 in. disk is cut from the sample with a cylindrical tool by spark machining. Thin slices, 5 mils thick, containing one row of fibers, are then, spark-machined from the disk. After spark machining, the slice is carefully polished with diamond paste until the row of fibers is exposed on each side, as shown in Figure 1.In the case where examination is desired with the electron beam parallel to the fiber, preparation is as follows: Experimental composites are usually 50 mils or less in thickness so an auxiliary holder is necessary during ion milling and for easy transfer to the electron microscope. This holder is pure aluminum sheet, 3 mils thick.

Direct Observation of Heat Exchanger Deposits by Cross Sectioning

1967 ◽  
Vol 25 ◽  
pp. 364-365
Author(s):  
R. W. Anderson ◽  
D. L. Senecal
Keyword(s):  
Electron Microscopy ◽  
Transmission Electron Microscopy ◽  
Heat Exchanger ◽  
Direct Observation ◽  
Cross Sections ◽  
Preparation Method ◽  
Specimen Preparation ◽  
Flowing Water ◽  
Transmission Electron ◽  
Deposit Layer

A problem was presented to observe the packing densities of deposits of sub-micron corrosion product particles. The deposits were 5-100 mils thick and had formed on the inside surfaces of 3/8 inch diameter Zircaloy-2 heat exchanger tubes. The particles were iron oxides deposited from flowing water and consequently were only weakly bonded. Particular care was required during handling to preserve the original formations of the deposits. The specimen preparation method described below allowed direct observation of cross sections of the deposit layers by transmission electron microscopy.The specimens were short sections of the tubes (about 3 inches long) that were carefully cut from the systems. The insides of the tube sections were first coated with a thin layer of a fluid epoxy resin by dipping. This coating served to impregnate the deposit layer as well as to protect the layer if subsequent handling were required.

Phase Transformations in Ti-7w/oMo-3w/oMe Alloy

1974 ◽  
Vol 32 ◽  
pp. 514-515
Author(s):  
S. Fujishiro
Keyword(s):  
Electron Microscopy ◽  
Mechanical Properties ◽  
Transmission Electron Microscopy ◽  
Tensile Strength ◽  
Mechanical Processing ◽  
Ray Method ◽  
X Ray ◽  
Transmission Electron ◽  
Water Quench ◽  
Alpha Beta

The mechanical properties of three titanium alloys (Ti-7Mo-3Al, Ti-7Mo- 3Cu and Ti-7Mo-3Ta) were evaluated as function of: 1) Solutionizing in the beta field and aging, 2) Thermal Mechanical Processing in the beta field and aging, 3) Solutionizing in the alpha + beta field and aging. The samples were isothermally aged in the temperature range 300° to 700*C for 4 to 24 hours, followed by a water quench. Transmission electron microscopy and X-ray method were used to identify the phase formed. All three alloys solutionized at 1050°C (beta field) transformed to martensitic alpha (alpha prime) upon being water quenched. Despite this heavily strained alpha prime, which is characterized by microtwins the tensile strength of the as-quenched alloys is relatively low and the elongation is as high as 30%.

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