Ovariectomy augments B lymphopoiesis and generation of monocyte-macrophage precursors in rat bone marrow

To investigate the effects of estrogen depletion on hematopoiesis and bone turnover, female rats were either ovariectomized (OVX) or sham operated and killed at 1, 2, 3, and 4 wk postsurgery. Flow cytometric analysis of bone marrow cells (BMC) revealed that, in close temporal association with the rise in bone turnover as measured by bone histomorphometry, the number of Thy 1.1+and KiB1R+BMC increased two- to threefold in OVX rats relative to sham controls. The Thy 1.1+BMC were further characterized as Thy 1.1+/KiB1R+and Thy 1.1+/HIS24+double-positive cells of the B cell lineage. A transient rise in ED1+myeloid cells expressing a lysosomal antigen specific for the monocyte-macrophage and osteoclast lineage coincided with the upregulation of osteoclast numbers in OVX rats at 2 wk postsurgery, but the number of ED8+myelomonocytic BMC remained unchanged. Administration of estradiol prevented the rise in Thy 1.1+, KiB1R+, and ED1+BMC in OVX animals. Our study indicates that ovariectomy upregulates B lymphopoiesis in rat bone marrow and increases myeloid cell differentiation into the monocyte-macrophage and possibly also the osteoclast lineage.

Download Full-text

Asialogangliosides as differentiation markers of rat erythropoietic and granulopoietic cells in rat bone marrow. Flow cytometric analysis of rat bone marrow cells using anti-asialoganglioside antibodies.

Cell Structure and Function ◽

10.1247/csf.11.89 ◽

1986 ◽

Vol 11 (2) ◽

pp. 89-97 ◽

Cited By ~ 4

Author(s):

Hirohisa Kimura ◽

Takao Taki ◽

Shyoji Hoshi ◽

Shyoji Yamamoto ◽

Makoto Matsumoto

Keyword(s):

Bone Marrow ◽

Bone Marrow Cells ◽

Flow Cytometric Analysis ◽

Differentiation Markers ◽

Flow Cytometric ◽

Rat Bone ◽

Marrow Cells

Download Full-text

A study on the investigation of cadmium chloride genotoxicity in rat bone marrow using micronucleus test and chromosome aberration analysis

Toxicology and Industrial Health ◽

10.1191/0748233705th237oa ◽

2005 ◽

Vol 21 (9) ◽

pp. 243-248 ◽

Cited By ~ 46

Author(s):

Ayla Çelik ◽

Ülkü Çömelekoğlu ◽

Serap Yalin

Keyword(s):

Bone Marrow ◽

Chronic Exposure ◽

Cadmium Chloride ◽

Bone Marrow Cells ◽

Micronucleus Test ◽

Genotoxic Effect ◽

Female Rats ◽

Tibia Bone ◽

Polychromatic Erythrocytes ◽

Rat Bone

In this study, we investigated the genotoxic and cytotoxic potential of cadmium chloride (CdCl2)in Wistar rat tibia bone marrow cells, using the structural chromosomal aberration (SCA) and micronucleus (MN) test systems. CdCl2 was administered to adult female rats as repeated i.p. doses of 0.5 mg/kg b.w. for 18 week (four months) at 48 h intervals. Mitomycin C (MMC) was used as a positive control (2 mg/kg b.w.). This study shows that cadmium chloride treatment significantly induced the frequency of micronucleus in polychromatic erythrocytes in tibia bone marrow. This increase in micronucleus frequency shows that cadmium has a genotoxic effect on bone marrow at this level. Also, in order to determine cytotoxicity in bone marrow, the ratio of polychromatic erythrocytes to normochromatic erythrocytes was calculated in bone marrow. The results of this study indicate that CdCl2 decreased this ratio. The decrease of this ratio in bone marrow shows CdCl2 may lead to cytotoxicity. We have reported that 0.5 mg/kg-level chronic exposure to cadmium (Cd) has an injurious effect on bone marrow. Our findings indicate that CdCl2 has a cytotoxic and genotoxic effect on rat bone marrow at chronic exposure.

Download Full-text

Effects of berberine on differentiation and bone resorption of osteoclasts derived from rat bone marrow cells

Journal of Chinese Integrative Medicine ◽

10.3736/jcim20090408 ◽

2009 ◽

Vol 7 (4) ◽

pp. 342-348 ◽

Cited By ~ 13

Author(s):

P Wei

Keyword(s):

Bone Marrow ◽

Bone Resorption ◽

Bone Marrow Cells ◽

Rat Bone ◽

Marrow Cells

Download Full-text

Glucosamine Protects Rat Bone Marrow Cells Against Cisplatin-induced Genotoxicity and Cytotoxicity

Anti-Cancer Agents in Medicinal Chemistry ◽

10.2174/1871520619666190704164126 ◽

2019 ◽

Vol 19 (14) ◽

pp. 1695-1702 ◽

Cited By ~ 1

Author(s):

Mohsen Cheki ◽

Salman Jafari ◽

Masoud Najafi ◽

Aziz Mahmoudzadeh

Keyword(s):

Bone Marrow ◽

Bone Marrow Cells ◽

Micronucleus Assay ◽

Ros Generation ◽

Polychromatic Erythrocytes ◽

Hematological Analysis ◽

Antagonistic Potential ◽

Harmful Effects ◽

Rat Bone ◽

Marrow Cells

Background and Objective: Glucosamine is a widely prescribed dietary supplement used in the treatment of osteoarthritis. In the present study, the chemoprotectant ability of glucosamine was evaluated against cisplatin-induced genotoxicity and cytotoxicity in rat bone marrow cells. Methods: Glucosamine was orally administrated to rats at doses of 75 and 150 mg/kg body weight for seven consecutive days. On the seventh day, the rats were treated with a single injection of cisplatin (5 mg/kg, i.p.) at 1h after the last oral administration. The cisplatin antagonistic potential of glucosamine was assessed by micronucleus assay, Reactive Oxygen Species (ROS) level analysis, hematological analysis, and flow cytometry. Results: Glucosamine administration to cisplatin-treated rats significantly decreased the frequencies of Micronucleated Polychromatic Erythrocytes (MnPCEs) and Micronucleated Normchromatic Erythrocytes (MnNCEs), and also increased PCE/(PCE+NCE) ratio in bone marrow cells. Furthermore, treatment of rats with glucosamine before cisplatin significantly inhibited apoptosis, necrosis and ROS generation in bone marrow cells, and also increased red blood cells count in peripheral blood. Conclusion: This study shows glucosamine to be a new effective chemoprotector against cisplatin-induced DNA damage and apoptosis in rat bone marrow cells. The results of this study may be helpful in reducing the harmful effects of cisplatin-based chemotherapy in the future.

Download Full-text