scholarly journals TNF Induces Choroid Plexus Epithelial Cell Barrier Alterations by Apoptotic and Nonapoptotic Mechanisms

2010 ◽  
Vol 2010 ◽  
pp. 1-10 ◽  
Author(s):  
Christian Schwerk ◽  
Kasia Rybarczyk ◽  
Frank Essmann ◽  
Annette Seibt ◽  
Marie-Louise Mölleken ◽  
...  
Keyword(s):  
Choroid Plexus ◽  
Inflammatory Diseases ◽  
High Mobility ◽  
Structural Basis ◽  
Choroid Plexus Epithelial Cell ◽  
The Central Nervous System ◽  
Cell Condensation ◽  
Dose Dependent Decrease ◽  
Dose Dependent ◽  
Choroid Plexus Epithelial

The choroid plexus epithelium constitutes the structural basis of the blood-cerebrospinal fluid barrier. Since the cytokine TNF is markedly increased during inflammatory diseases in the blood and the central nervous system, we investigated by which mechanisms TNF induces barrier alteration in porcine choroid plexus epithelial cells. We found a dose-dependent decrease of transepithelial electrical resistance, increase of paracellular inulin-flux, and induction of histone-associated DNA fragmentation and caspase-3 activation after TNF stimulation. This response was strongly aggravated by the addition of cycloheximide and could partially be inhibited by the NF-B inhibitor CAPE, but most effectively by the pan-caspase-inhibitor zVAD-fmk and not by the JNK inhibitor SP600125. Partial loss of cell viability could also be attenuated by CAPE. Immunostaining showed cell condensation and nuclear binding of high-mobility group box 1 protein as a sign of apoptosis after TNF stimulation. Taken together our findings indicate that TNF compromises PCPEC barrier function by caspase and NF-B dependent mechanisms.

scholarly journals Expression of junctional proteins in choroid plexus epithelial cell lines: a comparative study

2007 ◽  
Vol 4 (1) ◽  
pp. 11 ◽  
Author(s):  
Joanna Szmydynger-Chodobska ◽  
Crissey L Pascale ◽  
Andrew N Pfeffer ◽  
Cassaundra Coulter ◽  
Adam Chodobski
Keyword(s):  
Epithelial Cell ◽  
Comparative Study ◽  
Choroid Plexus ◽  
Cell Lines ◽  
Choroid Plexus Epithelial Cell ◽  
Junctional Proteins ◽  
Epithelial Cell Lines ◽  
Choroid Plexus Epithelial

scholarly journals TRIP6 functions in brain ciliogenesis

2021 ◽  
Vol 12 (1) ◽  
Author(s):  
Shalmali Shukla ◽  
Ronny Haenold ◽  
Pavel Urbánek ◽  
Lucien Frappart ◽  
Shamci Monajembashi ◽  
...  
Keyword(s):  
Choroid Plexus ◽  
Super Resolution ◽  
Epithelial Cell Line ◽  
Lim Domain ◽  
Choroid Plexus Epithelial Cell ◽  
Interaction Sites ◽  
Assembly Factor ◽  
Pericentriolar Material ◽  
Choroid Plexus Epithelial

AbstractTRIP6, a member of the ZYXIN-family of LIM domain proteins, is a focal adhesion component. Trip6 deletion in the mouse, reported here, reveals a function in the brain: ependymal and choroid plexus epithelial cells are carrying, unexpectedly, fewer and shorter cilia, are poorly differentiated, and the mice develop hydrocephalus. TRIP6 carries numerous protein interaction domains and its functions require homodimerization. Indeed, TRIP6 disruption in vitro (in a choroid plexus epithelial cell line), via RNAi or inhibition of its homodimerization, confirms its function in ciliogenesis. Using super-resolution microscopy, we demonstrate TRIP6 localization at the pericentriolar material and along the ciliary axoneme. The requirement for homodimerization which doubles its interaction sites, its punctate localization along the axoneme, and its co-localization with other cilia components suggest a scaffold/co-transporter function for TRIP6 in cilia. Thus, this work uncovers an essential role of a LIM-domain protein assembly factor in mammalian ciliogenesis.

scholarly journals The Cardiovascular Effects of Central Hydrogen Sulfide Are Related to KATP Channels Activation

2011 ◽  
pp. 729-738 ◽  
Author(s):  
W.-Q. LIU ◽  
C. CHAI ◽  
X.-Y. LI ◽  
W.-J. YUAN ◽  
W.-Z. WANG ◽  
...  
Keyword(s):  
Blood Pressure ◽  
Hydrogen Sulfide ◽  
Katp Channel ◽  
Channel Blocker ◽  
Cardiovascular Effects ◽  
Katp Channels ◽  
Channel Activation ◽  
The Central Nervous System ◽  
Dose Dependent Decrease ◽  
Dose Dependent

Hydrogen sulfide (H2S), an endogenous “gasotransmitter”, exists in the central nervous system. However, the central cardiovascular effects of endogenous H2S are not fully determined. The present study was designed to investigate the central cardiovascular effects and its possible mechanism in anesthetized rats. Intracerebroventricular (icv) injection of NaHS (0.17~17 μg) produced a significant and dose-dependent decrease in blood pressure (BP) and heart rate (HR) (P<0.05) compared to control. The higher dose of NaHS (17 μg, n=6) decreased BP and HR quickly of rats and 2 of them died of respiratory paralyse. Icv injection of the cystathionine beta-synthetase (CBS) activator s-adenosyl-L-methionine (SAM, 26 μg) also produced a significant hypotension and bradycardia, which were similar to the results of icv injection of NaHS. Furthermore, the hypotension and bradycardia induced by icv NaHS were effectively attenuated by pretreatment with the KATP channel blocker glibenclamide but not with the CBS inhibitor hydroxylamine. The present study suggests that icv injection of NaHS produces hypotension and bradycardia, which is dependent on the KATP channel activation.

scholarly journals Anti-diarrhoeal and CNS Depressant Activity of Methanolic Extract of Saccharum spontaneum Linn.

1970 ◽  
Vol 1 (1) ◽  
pp. 63-68 ◽  
Author(s):  
Md Mynol Islam Vhuiyan ◽  
Israt Jahan Biva ◽  
Moni Rani Saha ◽  
Muhammad Shahidul Islam
Keyword(s):  
Castor Oil ◽  
Methanolic Extract ◽  
Experimental Models ◽  
Saccharum Spontaneum ◽  
Cns Depressant ◽  
Whole Plant ◽  
The Central Nervous System ◽  
Dose Dependent Decrease ◽  
Cns Depressant Activity ◽  
Dose Dependent

Preliminary phytochemical screening of the methanolic extract of the whole plant of Saccharum spontaneum Linn. (Family- Gramineae) revealed the presence of alkaloids, flavonoids, reducing sugar, tannins and saponins. The antidiarrhoeal activity of the extract (200 and 400 mg/kg) was assessed on experimental animal and a dose dependent decrease in the total number of faecal dropping was obsereved in castor oil induced diarrhoea in mice. The plant extract was also assessed for effect on the central nervous system (CNS) using a number of neuropharmacological experimental models in mice. The extract produced a dosedependent reduction of the onset and duration of pentobarbitone-induced hypnosis, reduction of locomotor and exploratory activities in the open field and hole cross tests. These results suggest that the extract possesses antidiarrhoeal and CNS depressant activity. Key Words: Saccharum spontaneum, Castor oil, Loperamide, Pentobarbitone.     doi:10.3329/sjps.v1i1.1810 S. J. Pharm. Sci. 1(1&2): 63-68

scholarly journals Fgf2 is expressed in human and murine embryonic choroid plexus and affects choroid plexus epithelial cell behaviour

2008 ◽  
Vol 5 (1) ◽  
pp. 20 ◽  
Author(s):  
Sarah Greenwood ◽  
Adam Swetloff ◽  
Angela M Wade ◽  
Tetsuya Terasaki ◽  
Patrizia Ferretti
Keyword(s):  
Epithelial Cell ◽  
Choroid Plexus ◽  
Choroid Plexus Epithelial Cell ◽  
Cell Behaviour ◽  
Choroid Plexus Epithelial
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