scholarly journals Determining the Effect of Calculus, Hypocalcification, and Stain on Using Optical Coherence Tomography and Polarized Raman Spectroscopy for Detecting White Spot Lesions

2010 ◽  
Vol 2010 ◽  
pp. 1-7 ◽  
Author(s):  
Amanda Huminicki ◽  
Cecilia Dong ◽  
Blaine Cleghorn ◽  
Michael Sowa ◽  
Mark Hewko ◽  
...  
Keyword(s):  
Raman Spectroscopy ◽  
Optical Coherence Tomography ◽  
Ex Vivo ◽  
White Spot ◽  
Optical Methods ◽  
Optical Coherence ◽  
The Mean ◽  
Polarized Raman Spectroscopy ◽  
Polarized Raman ◽  
Depolarization Ratios

Optical coherence tomography (OCT) and polarized Raman spectroscopy (PRS) have been shown as useful methods for distinguishing sound enamel from carious lesionsex vivo. However, factors in the oral environment such as calculus, hypocalcification, and stain could lead to false-positive results. OCT and PRS were used to investigate extracted human teeth clinically examined for sound enamel, white spot lesion (WSL), calculus, hypocalcification, and stain to determine whether these factors would confound WSL detection with these optical methods. Results indicate that OCT allowed differentiating caries from sound enamel, hypocalcification, and stain, with calculus deposits recognizable on OCT images. ANOVA and post-hoc unequal N HSD analyses to compare the mean Raman depolarization ratios from the various groups showed that the mean values were statistically significant atP<.05, except for several comparison pairs. With the current PRS analysis method, the mean depolarization ratios of stained enamel and caries are not significantly different due to the sloping background in the stained enamel spectra. Overall, calculus and hypocalcification are not confounding factors affecting WSL detection using OCT and PRS. Stain does not influence WSL detection with OCT. Improved PRS analysis methods are needed to differentiate carious from stained enamel.

scholarly journals Morpho-molecular ex vivo detection and grading of non-muscle-invasive bladder cancer using forward imaging probe based multimodal optical coherence tomography and Raman spectroscopy

The Analyst ◽  
2020 ◽  
Vol 145 (4) ◽  
pp. 1445-1456 ◽  
Author(s):  
Fabian Placzek ◽  
Eliana Cordero Bautista ◽  
Simon Kretschmer ◽  
Lara M. Wurster ◽  
Florian Knorr ◽  
...  
Keyword(s):  
Raman Spectroscopy ◽  
Optical Coherence Tomography ◽  
Imaging System ◽  
Ex Vivo ◽  
Large Field ◽  
Optical Coherence ◽  
Fiber Optic Probe ◽  
Optic Probe ◽  
Muscle Invasive

Characterization of bladder biopsies, using a combined fiber optic probe-based optical coherence tomography and Raman spectroscopy imaging system that allows a large field-of-view imaging and detection and grading of cancerous bladder lesions.

scholarly journals Optical coherence tomography and Raman spectroscopy of the ex-vivo retina

2009 ◽  
Vol 2 (6-7) ◽  
pp. 398-406 ◽  
Author(s):  
Julia W. Evans ◽  
Robert J. Zawadzki ◽  
Rui Liu ◽  
James W. Chan ◽  
Stephen M. Lane ◽  
...  
Keyword(s):  
Raman Spectroscopy ◽  
Optical Coherence Tomography ◽  
Ex Vivo ◽  
Optical Coherence

scholarly journals The Optical Coherence Tomography and Raman Spectroscopy for Sensing of the Bone Demineralization Process

Sensors ◽  
2021 ◽  
Vol 21 (19) ◽  
pp. 6468
Author(s):  
Maciej J. Głowacki ◽  
Aleksandra M. Kamińska ◽  
Marcin Gnyba ◽  
Jerzy Pluciński ◽  
Marcin R. Strąkowski
Keyword(s):  
Raman Spectroscopy ◽  
Optical Coherence Tomography ◽  
Bone Mineralization ◽  
Optical Methods ◽  
Optical Coherence ◽  
Bone Demineralization ◽  
Raman Measurement ◽  
Preliminary Study ◽  
Optical Examination ◽  
Bone Condition

The presented research was intended to seek new optical methods to investigate the demineralization process of bones. Optical examination of the bone condition could facilitate clinical trials and improve the safety of patients. The authors used a set of complementary methods: polarization-sensitive optical coherence tomography (PS-OCT) and Raman spectroscopy. Chicken bone samples were used in this research. To stimulate in laboratory conditions the process of demineralization and gradual removal of the hydroxyapatite, the test samples of bones were placed into 10% acetic acid. Measurements were carried out in two series. The first one took two weeks with data acquired every day. In the second series, the measurements were made during one day at an hourly interval (after 1, 2, 3, 5, 7, 10, and 24 h). The relation between the content of hydroxyapatite and images recorded using OCT was analyzed and discussed. Moreover, the polarization properties of the bones, including retardation angles of the bones, were evaluated. Raman measurement confirmed the disappearance of the hydroxyapatite and the speed of this process. This work presents the results of the preliminary study on the possibility of measuring changes in bone mineralization by means of the proposed methods and confirms their potential for practical use in the future.

scholarly journals Automatic intraoperative optical coherence tomography positioning

2020 ◽  
Vol 15 (5) ◽  
pp. 781-789 ◽  
Author(s):  
Matthias Grimm ◽  
Hessam Roodaki ◽  
Abouzar Eslami ◽  
Nassir Navab
Keyword(s):  
Optical Coherence Tomography ◽  
Standard Deviation ◽  
Ex Vivo ◽  
Anterior Segment ◽  
Semantic Segmentation ◽  
Recognition Algorithm ◽  
Optical Coherence ◽  
Cross Sectional ◽  
Intraoperative Optical Coherence Tomography ◽  
The Mean

Abstract Purpose Intraoperative optical coherence tomography (iOCT) was recently introduced as a new modality for ophthalmic surgeries. It provides real-time cross-sectional information at a very high resolution. However, properly positioning the scan location during surgery is cumbersome and time-consuming, as a surgeon needs both his hands for surgery. The goal of the present study is to present a method to automatically position an iOCT scan on an anatomy of interest in the context of anterior segment surgeries. Methods First, a voice recognition algorithm using a context-free grammar is used to obtain the desired pose from the surgeon. Then, the limbus circle is detected in the microscope image and the iOCT scan is placed accordingly in the X–Y plane. Next, an iOCT sweep in Z direction is conducted and the scan is placed to centre the topmost structure. Finally, the position is fine-tuned using semantic segmentation and a rule-based system. Results The logic to position the scan location on various anatomies was evaluated on ex vivo porcine eyes (10 eyes for corneal apex and 7 eyes for cornea, sclera and iris). The mean euclidean distances (± standard deviation) was 76.7 (± 59.2) pixels and 0.298 (± 0.229) mm. The mean execution time (± standard deviation) in seconds for the four anatomies was 15 (± 1.2). The scans have a size of 1024 by 1024 pixels. The method was implemented on a Carl Zeiss OPMI LUMERA 700 with RESCAN 700. Conclusion The present study introduces a method to fully automatically position an iOCT scanner. Providing the possibility of changing the OCT scan location via voice commands removes the burden of manual device manipulation from surgeons. This in turn allows them to keep their focus on the surgical task at hand and therefore increase the acceptance of iOCT in the operating room.

Classification of burn injury using Raman spectroscopy and optical coherence tomography: An ex-vivo study on porcine skin

Burns ◽  
2019 ◽  
Vol 45 (3) ◽  
pp. 659-670 ◽  
Author(s):  
Lakshmi Priya Rangaraju ◽  
Gautam Kunapuli ◽  
Dayna Every ◽  
Oscar D. Ayala ◽  
Priya Ganapathy ◽  
...  
Keyword(s):  
Raman Spectroscopy ◽  
Optical Coherence Tomography ◽  
Burn Injury ◽  
Ex Vivo ◽  
Optical Coherence ◽  
Porcine Skin ◽  
Ex Vivo Study

Ex-vivo evaluation of an early caries detector based on integrated OCT and polarized Raman spectroscopy (Conference Presentation)

2017 ◽  
Author(s):  
Guy Lamouche ◽  
Christian Padioleau ◽  
Mark Hewko ◽  
Michael S. D. Smith ◽  
Bernhard J. Schattka ◽  
...  
Keyword(s):  
Raman Spectroscopy ◽  
Ex Vivo ◽  
Polarized Raman Spectroscopy ◽  
Polarized Raman ◽  
Early Caries

Ex vivo detection and characterization of early dental caries by optical coherence tomography and Raman spectroscopy

2005 ◽  
Vol 10 (3) ◽  
pp. 031118 ◽  
Author(s):  
Alex C.-T. Ko ◽  
Lin-P’ing Choo-Smith ◽  
Mark Hewko ◽  
Lorenzo Leonardi ◽  
Michael G. Sowa ◽  
...  
Keyword(s):  
Raman Spectroscopy ◽  
Optical Coherence Tomography ◽  
Dental Caries ◽  
Ex Vivo ◽  
Optical Coherence

scholarly journals The Optical Coherence Tomography and Raman Spectroscopy for Sensing of the Bone Demineralization Process

2021 ◽  
Author(s):  
Maciej Głowacki ◽  
Aleksandra Kamińska ◽  
Marcin Gnyba ◽  
Jerzy Pluciński ◽  
Marcin Robert Strąkowski
Keyword(s):  
Raman Spectroscopy ◽  
Optical Coherence Tomography ◽  
Optical Measurement ◽  
Measurement Techniques ◽  
Optical Methods ◽  
Optical Coherence ◽  
Bone Demineralization ◽  
Optical Examination ◽  
Bone Condition ◽  
Non Destructive

Aim of the presented research was to develop an optical sensing system to investigate the demineralization process of the bones. Optical measurement techniques are widely used and increasingly adapted in biological and biomedical applications due to their non-destructive nature and safety. Optical examination of the bone condition could facilitate clinical trials and improve the safety of patients. The authors used a set of complementary methods: polarization-sensitive optical coherence tomography (PS-OCT) and Raman spectroscopy. To stimulate the process of demineralization and gradual removal of the hydroxyapatite, the test samples of chicken bones were placed into 10% acetic acid. Measurements were carried out in two series. The first one took two weeks with data acquired every day. In the second series, the measurements were made during one day at an hourly interval (after 1, 2, 3, 5, 7, 10, and 24 hours). Raman spectroscopy was used to evaluate the disappearance of the hydroxyapatite. The relation between the content of hydroxyapatite and images recorded using OCT was analyzed and discussed. Moreover, the polarization properties of the bones have been evaluated. Based on OCT images, the retardation angles of the bones have been calculated. This work presents a preliminary study on the mechanism of bone demineralization and confirms the potential of the applied optical methods.

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