scholarly journals Ultrasensitive detection of genomic DNA from apple leaf tissues, using surface-enhanced Raman scattering

2011 ◽  
Vol 25 (1) ◽  
pp. 33-43 ◽  
Author(s):  
Cristina M. Muntean ◽  
Nicolae Leopold ◽  
Adela Halmagyi ◽  
Sergiu Valimareanu

Ultrasensitive detection of nucleic acids is demonstrated through exploiting the effect of surface-enhanced Raman scattering (SERS). In this work the SERS spectra of eight genomic DNAs from leaves of apple trees grown in the field (Malus domesticaBorkh., Fam.Rosaceae, cultivars. Florina, Idared, Rebra, Goldrush, Romus 3, Romus 4 and the rootstocks M9 and M26) have been analyzed in the wavenumber range 200–1800 cm–1. SERS signatures, spectroscopic band assignments and structural interpretations of these plant genomic DNAs are reported. SERS spectra of nucleic acids are compared here with caution, because these signals are time-dependent and are strongly influenced by DNA amount in the measured sample volume. Similarities of the SERS spectra of genomic DNAs extracted from apple leaves of Goldrush, Rebra and Florina cultivars, respectively, have been detected. Besides, the SERS spectra, corresponding to DNA from M9, Romus 4 and M26, leaves, respectively, show similar features and well resolved bands. Based on this work, specific plant DNA-ligand interactions or DNA structural changes induced by plant stress conditions associated with their natural environment, might be further investigated using surface-enhanced Raman spectroscopy.

2015 ◽  
Vol 69 (12) ◽  
pp. 1487-1495 ◽  
Author(s):  
Qiu Lu ◽  
Tao Lang ◽  
Shuguo Fan ◽  
Wen Chen ◽  
Deqing Zang ◽  
...  

The Analyst ◽  
2020 ◽  
Vol 145 (23) ◽  
pp. 7662-7672
Author(s):  
Thakshila Liyanage ◽  
Adrianna N. Masterson ◽  
Sumon Hati ◽  
Greta Ren ◽  
Nicholas E. Manicke ◽  
...  

Nanoplasmonic superlattice surface-enhanced Raman scattering substrates have been developed for an ultrasensitive detection of fentanyl and cocaine from patients’ plasma.


2015 ◽  
Vol 51 (33) ◽  
pp. 7152-7155 ◽  
Author(s):  
O. O. Alabi ◽  
A. N. F. Edilbi ◽  
C. Brolly ◽  
D. Muirhead ◽  
J. Parnell ◽  
...  

Surface enhanced Raman spectroscopy using a gold substrate and excitation at 514 nm can detect sub parts per million quantities of asphaltene and thereby petroleum.


The Analyst ◽  
2014 ◽  
Vol 139 (13) ◽  
pp. 3352-3355 ◽  
Author(s):  
Michael Driver ◽  
Yue Li ◽  
Jinkai Zheng ◽  
Eric Decker ◽  
David Julian McClements ◽  
...  

A simple fabrication method for preparing lipophilic gold nanoparticles (AuNPs) suitable for use as substrates in surface-enhanced Raman scattering (SERS) applications of lipids was developed.


2020 ◽  
Vol 15 (11) ◽  
pp. 1321-1326
Author(s):  
Aning Ma ◽  
Wenjing Wei ◽  
Zhongqiang Zhang ◽  
Sichang Peng ◽  
Yurong Wang ◽  
...  

An efficient surface-enhanced Raman scattering substrate based on silver nanoparticles/silicon pyramid arrays structure is theoretically investigated and experimentally demonstrated. The electric field distributions using finite-element-method are calculated. The surface-enhanced Raman scattering behaviors of sensitivity, uniformity and stability are emphatically discussed and compared by the detection of crystal violet. These theoretical and experimental results reveal that the silver nanoparticles/silicon pyramid arrays substrate is expected to be an effective surface-enhanced Raman scattering platform for label-free sensitive surfaceenhanced Raman spectroscopy detection in areas of biotechnology, medicine and food safety.


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