scholarly journals Extractive Spectrophotometric Method for the Determination of Lamivudine and Zidovudine in Pharmaceutical Preparations Using Bromocresol Purple

2013 ◽  
Vol 2013 ◽  
pp. 1-5
Author(s):  
Serife Evrim Kepekci Tekkeli
Keyword(s):  
Quality Control ◽  
Spectrophotometric Method ◽  
Pharmaceutical Preparations ◽  
Pharmaceutical Formulations ◽  
Hplc Method ◽  
Ion Pair ◽  
Bromocresol Purple ◽  
Reaction Conditions ◽  
Drug Substances

A new spectrophotometric method has been established for the quantitation of lamivudine (LVD) and zidovudine (ZVD) in pharmaceutical preparations. The method is based on the reaction between the investigated drug substances and bromocresol purple (BCP) producing ion-pair complexes in acidic buffers which are suitable for chloroform extraction. The maximum absorbance of these complexes was measured at 424 nm in chloroform. All variables were studied to optimize the reaction conditions. Linearity ranges were found to be 25–250 μg mL−1for LVD-BCP and 50–300 μg mL−1for ZVD-BCP. The developed method was successfully applied for the determination of these drugs in pharmaceutical preparations. Excipients in pharmaceutical formulations did not interfere in the analysis. The results were compared statistically with those obtained by the HPLC method reported in the literature. According to the results, the proposed method can be recommended for quality control and routine analysis.

scholarly journals Extractive Spectrophotometric Method for Determination of Pioglitazone Hydrochloride in Raw Material and Tablets Using Ion-Pair Formation

2010 ◽  
Vol 7 (3) ◽  
pp. 915-921 ◽  
Author(s):  
Massoud Amanlou ◽  
Mohadeseh Zarei-Ghobadi ◽  
Mohammad Kazem Rofouei ◽  
Shahrooz Saremi ◽  
Abbas Kebriaeezadeh
Keyword(s):  
Spectrophotometric Method ◽  
Pharmaceutical Formulations ◽  
Hplc Method ◽  
Ion Pair ◽  
Raw Material ◽  
Bromocresol Green ◽  
Assay Procedure ◽  
Significant Difference ◽  
Significant Interference

A simple, rapid and extractive spectrophotometric method was developed for the determination of pioglitazone hydrochloride in pure and pharmaceutical formulations. This method is based on the formation of yellow ion-pair complex between the basic nitrogen of the drug and bromocresol green (BCG) in phthalate buffer of pH 2.4. The formed complexes were extracted with chloroform and measured at 419 nm. The analytical parameters and their effects on the proposed systems are investigated. Beer’s law was obeyed in the range 2.5-14 μg/mL with correlation coefficient ≥ 0.995. The proposed method has been applied successfully for the determination of drug in commercial tablets dosage forms. No significant interference was observed from the excipients commonly used as pharmaceutical aids with the assay procedure. The validity of the proposed method was established by parallel determination against HPLC method and there was no significant difference between these two methods.

scholarly journals DEVELOPMENT AND VALIDATION OF SPECTROPHOTOMETRIC METHOD FOR THE DETERMINATION OF DPP-4 INHIBITOR, SITAGLIPTIN, IN ITS PHARMACEUTICAL PREPARATIONS

2018 ◽  
Vol 35 (3) ◽  
pp. 45
Author(s):  
C. Bala Sekaran ◽  
A. Prameela Rani
Keyword(s):  
Spectrophotometric Method ◽  
Pharmaceutical Preparations ◽  
Pharmaceutical Formulations ◽  
Molar Absorptivity ◽  
Amino Group ◽  
Reaction Conditions ◽  
Colored Product ◽  
Primary Amino ◽  
Development And Validation

A simple, sensitive and reproducible spectrophotometric method was developed for the determination of sitagliptin phosphate in bulk and in pharmaceutical formulations. The proposed method is based on condensation of the primary amino group of sitagliptin phosphate with acetyl acetone and formaldehyde producing a yellow colored product, which is measured spectrophotometrically at 430nm. The color was stable for about 1 hour. Beer’s law is obeyed over a concentration range of 5-25 μg/ml. The apparent molar absorptivity and Sandell sensitivity values are 1.067 x 104 Lmol-1cm-1 and 0.0471 μgcm-2 respectively. All the variables were studied to optimize the reaction conditions. No interference was observed in the presence of common pharmaceutical excipients. The validity of the method was tested by analyzing sitagliptin phosphate in its pharmaceutical preparations. Good recoveries were obtained. The developed method was successfully employed for the determination of sitagliptin phosphate in various pharmaceutical preparations.

scholarly journals New Kinetic Spectrophotometric Method for Determination of Fexofenadine Hydrochloride in Pharmaceutical Formulations

2014 ◽  
Vol 2014 ◽  
pp. 1-8 ◽  
Author(s):  
Safwan Ashour ◽  
Mouhammed Khateeb
Keyword(s):  
Spectrophotometric Method ◽  
Initial Rate ◽  
Pharmaceutical Preparations ◽  
Pharmaceutical Formulations ◽  
Fixed Time ◽  
Official Method ◽  
Reaction Conditions ◽  
Accuracy And Precision ◽  
Kinetic Spectrophotometric

A simple and sensitive kinetic spectrophotometric method was developed for the determination of fexofenadine hydrochloride in bulk and pharmaceutical preparations. The method is based on a kinetic investigation of the oxidation reaction of fexofenadine using alkaline potassium permanganate as an oxidizing agent at room temperature. The reaction is followed spectrophotometrically by measuring the increase of absorbance owing to the formation of manganate ion at 610 nm. The initial rate and fixed time (at 15 min) methods are utilized for construction of calibration graphs. All the reaction conditions for the proposed method have been studied. The linearity range was found to be 2.5–50.0 μg mL−1 with detection limit of 0.055 μg mL−1 for both initial rate and fixed time methods. The proposed method was applied successfully for the determination of fexofenadine in pharmaceutical formulations; the percentage recoveries were 99.98–101.96%. The results obtained were compared statistically with those obtained by the official method and showed no significant differences regarding accuracy and precision.

scholarly journals Microanalysis of Selected NSAIDs Using the Spectrophotometric Method

Eng ◽  
2020 ◽  
Vol 1 (2) ◽  
pp. 211-221
Author(s):  
Paweł Gumułka ◽  
Monika Dąbrowska ◽  
Małgorzata Starek
Keyword(s):  
Quality Control ◽  
Spectrophotometric Method ◽  
Pharmaceutical Preparations ◽  
Pharmacokinetic Studies ◽  
Over The Counter ◽  
Nsaid Group ◽  
Inflammatory Drugs ◽  
Drug Quality Control ◽  
Uv Range

Non-steroidal anti-inflammatory drugs (NSAIDs) are the group of drugs most commonly used in medicine. They are available over the counter to treat fevers and pains of various origins. The clinical and pharmaceutical analysis of these drugs requires effective analytical procedures for drug quality control, pharmacodynamic and pharmacokinetic studies. This article presents the spectrophotometric method that was used to analyze selected drugs from the NSAID group. The conditions for the determination of selected coxibs and oxicams in the UV range with the use of microplates have been developed. The presented procedure has been validated in accordance with the requirements, guaranteeing reliable results. The obtained results give the basis for the conclusion that the method can be successfully used in the quality control of pharmaceutical preparations with a small amount of available sample.

scholarly journals Spectrophotometric and Conductometric Determination of Clomiphene Citrate and Nefazodone HCl

2008 ◽  
Vol 5 (s2) ◽  
pp. 1069-1080 ◽  
Author(s):  
Wafaa S. Hassan ◽  
Mervat M. Hosny
Keyword(s):  
Spectrophotometric Method ◽  
Clomiphene Citrate ◽  
Pharmaceutical Preparations ◽  
Conductometric Titration ◽  
Ion Pair ◽  
Experimental Conditions ◽  
Relative Standard ◽  
Colored Complexes ◽  
Standard Deviations

Two accurate, rapid and simple spectrophotometric and conductometric methods were developed for the determination of clomiphene citrate (CMP) and nefazodone HCl (NFZ), the proposed methods depends upon the reaction of ammonium reineckate with the two studied drugs to form stable precipitate of ion-pair complexes, which was dissolved in suitable solvent. The pink colored complexes were determined colorimetrically at 509, 523.6 nm, respectively. Using the conductometric titration, the studied drugs could be evaluated in 50% (v/v) acetone in the range 60.02-540.18 and 63.3-443.1 μg mL-1for clomiphene citrate and nefazodone HCl, respectively. While for spectrophotometric method the ranges were 0.2-1.8 and 0.2-1.6 mg mL-1for clomiphene citrate and nefazodone HCl respectively. Various experimental conditions were studied. The results obtained showed good recoveries with relative standard deviations of 0.759 and 0.552%. The proposed procedures were applied successfully to the analysis of these drugs in their pharmaceutical preparations and the results were favourably comparable with the official and reference methods. The molar combining ratio reveal that (1:1) (drug : reagent) ion associates were formed.

scholarly journals Determination of loratadine in pharmaceuticals by a spectrophotometric method

2015 ◽  
Vol 26 (1) ◽  
pp. 27-31
Author(s):  
Georgeta Pavalache ◽  
Nicoleta Matei ◽  
Antoanela Popescu
Keyword(s):  
Quality Control ◽  
Spectrophotometric Method ◽  
Reading Time ◽  
Pharmaceutical Formulations ◽  
Mixed Composition ◽  
Oral Formulations ◽  
Short Time

Abstract The spectrophotometric method for determination of loratadine using tetraiodomercurate has been applied in various pharmaceutical formulations. The results confirmed that recovery value is optimum and the method is valid, thus it can be used in quality control and evaluation of loratadine tablets, oral formulations of mixed composition, oral solutions, etc. The method is easy and simple to apply, does not require complicated equipment and spectrophotometric reading time is reduced, which allows a large number of analyzes in a relatively short time.

scholarly journals Indirect Spectrophotometric Methods for the Determination of Tadalafil

2015 ◽  
Vol 1 (2) ◽  
pp. 11 ◽  
Author(s):  
Safwan Mohammad Fraihat
Keyword(s):  
Oxidation Reaction ◽  
Indigo Carmine ◽  
Pharmaceutical Preparations ◽  
Pharmaceutical Formulations ◽  
Blue Dye ◽  
Reaction Products ◽  
Reaction Conditions ◽  
Factors Affecting ◽  
Spectrophotometric Methods

Two spectrophotometric methods were developed for the determination Tadalafil in pharmaceutical preparations. The methods are based on the oxidation reaction with known excess amount of Ce(IV) and estimation of the unreacted amount using Indigo carmine dye (Method A) and in Methylene blue dye  (Method B). the factors affecting the reaction conditions were studied and the absorbance of absorbance of the oxidation reaction products were monitored at 610 and 600 nm for methods A and B respectively. Beer's law is obeyed in the concentration ranges 11 to 50 and 10 to 55 ppm, the limits of detection and quantification are reported. The proposed method was applied to the determination of the drug in pharmaceutical formulations and the results demonstrated that the method is equally accurate, precise and reproducible as the official methods. The validity of method was established by recovery studies with satisfactory results.

scholarly journals Development of Analytical Profile of Lamotrigine and its API Formulation

2021 ◽  
pp. 111-114
Author(s):  
VISHAL CHAUDHARY ◽  
VASUNDHARA SAXENA
Keyword(s):  
Spectrophotometric Method ◽  
Quantitative Estimation ◽  
Pharmaceutical Formulations ◽  
Hplc Method ◽  
Active Pharmaceutical Ingredients ◽  
Pharmaceutical Ingredients ◽  
Rp Hplc ◽  
System Suitability ◽  
Bulk Drug

Objective: The objective of this review is to put a light on the development of lamotrigine and its active pharmaceutical ingredients formulation with proper demonstration. Method: In the present work, one of the most imperative spectrophotometric method which is RP-HPLC method has been developed for the quantitative estimation of lamotrigine in bulk and pharmaceutical formulations. UV spectrophotometric method which involves the determination of Lamotrigine in bulk and in bulk drug and pharmaceutical formulation has maximum absorption at 307.5nm in methanol. It obeys Beer’s and Lambert’s law in the concentration range of 5-45 µg/ml. A rapid and sensitive RP- HPLC Method with UV detection (270 nm) for routine analysis of Lamotrigine formulation was developed. Chromatography was performed with mobile phase containing a mixture of methanol and Phosphate buffer (65:35v/v) with flow rate 1.0 ml/min. In the range of 20-100 µg/ml, the linearity of lamotrigine shows a correlation co-efficient of 0.9998. The proposed method was validated by determining sensitivity and system suitability parameters.

scholarly journals Validated Extractive Spectrophotometric Method for Determination of Domperidone in Pharmaceutical Formulations

2015 ◽  
Vol 17 (1) ◽  
pp. 25-31
Author(s):  
Jasmin Shah ◽  
M Rasul Jan ◽  
Muhammad Tariq Shah
Keyword(s):  
Acidic Medium ◽  
Pharmaceutical Formulations ◽  
Pharmaceutical Journal ◽  
Bromothymol Blue ◽  
Hplc Method ◽  
Ion Pair ◽  
Bromophenol Blue ◽  
Spectrophotometric Methods ◽  
New Methods

Simple, precise and sensitive extractive spectrophotometric methods have been developed for the determination of domperidone in pharmaceutical formulations. The new methods involve the formation of colored extractable ion pair complexes of the drug with bromothymol blue (BTB) and bromophenol blue (BPB) in acidic medium. The effects of various parameters like pH, reagent concentration and shaking time were studied. The extracted complexes of domperidone showed maximum absorbance at 410 nm with BTB and at 415 nm with BPB dye. The stiochiometry of the reaction between domperidone, BTB and BPB was found to be 1: 4. Domperidone was found to obey Beer’s law in the concentration ranges of 0.6-35 ?g/ml, 1-30 ?g/ml with BTB and BPB, respectively. The method has been applied successfully for the determination of domperidone in commercial tablets and suspension samples. The results obtained by the proposed methods were validated statistically and compared with the official HPLC method. DOI: http://dx.doi.org/10.3329/bpj.v17i1.22310 Bangladesh Pharmaceutical Journal 17(1): 25-31, 2014

scholarly journals Analytical Methods for Determination of Muscle Relax-ant Thiocolchicoside in Pharmaceutical Preparations- A Review

2015 ◽  
Vol 2 (1) ◽  
pp. 43-55 ◽  
Author(s):  
J.K. Rajput ◽  
P.H. Patil ◽  
S. J. Surana ◽  
A. A. Shirkhedkar
Keyword(s):  
Analytical Methods ◽  
Bulk Material ◽  
Pharmaceutical Preparations ◽  
Pharmaceutical Formulations ◽  
Low Back ◽  
Drug Substances ◽  
Uv Visible ◽  
Immune Assays ◽  
Acting Muscle

Thiocolchicoside is a centrally acting muscle relaxant and used in combination with many NSAIDs for the treatment of various musculoskeletal disorders. As it is less sedative than other centrally acting muscle relaxants hence frequently prescribed for low back pain (LBP), orthopedic, traumatic and rheumatologic disorders. It is available in market in single component and as multicomponent formulations. Various analytical methods are available for determination of thiocolchico-side in drug substances and formulated products. The present article summarizes more than 100 analytical methods including all types of chromatographic, UV-Visible spectrophotometry and radio immune assays with their percentage of utility for de-termination of thiocolchicoside in biological matrices, bulk material and different pharmaceutical formulations.

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