Synthesis, Structural Characterization, and Biological Activity Studies of Ni(II) and Zn(II) Complexes

Ni(II) and Zn(II) complexes were synthesized from tridentate 3-formyl chromone Schiff bases such as 3-((2-hydroxyphenylimino)methyl)-4H-chromen-4-one (HL1), 2-((4-oxo-4H-chromen-3-yl)methylneamino)benzoic acid (HL2), 3-((3-hydroxypyridin-2-ylimino)methyl)-4H-chromen-4-one (HL3), and 3-((2-mercaptophenylimino)methyl)-4H-chromen-4-one (HL4). All the complexes were characterized in the light of elemental analysis, molar conductance, FTIR, UV-VIS, magnetic, thermal, powder XRD, and SEM studies. The conductance and spectroscopic data suggested that, the ligands act as neutral and monobasic tridentate ligands and form octahedral complexes with general formula [M(L1–4)2]·nH2O (M = Ni(II) and Zn(II)). Metal complexes exhibited pronounced activity against tested bacteria and fungi strains compared to the ligands. In addition metal complexes displayed good antioxidant and moderate nematicidal activities. The cytotoxicity of ligands and their metal complexes have been evaluated by MTT assay. The DNA cleavage activity of the metal complexes was performed using agarose gel electrophoresis in the presence and absence of oxidant H2O2. All metal complexes showed significant nuclease activity in the presence of H2O2.

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A YoeB toxin cleaves both RNA and DNA

Scientific Reports ◽

10.1038/s41598-021-82950-6 ◽

2021 ◽

Vol 11 (1) ◽

Author(s):

Julia McGillick ◽

Jessica R. Ames ◽

Tamiko Murphy ◽

Christina R. Bourne

Keyword(s):

Dna Cleavage ◽

Divalent Cations ◽

Nuclease Activity ◽

Dose Dependence ◽

Cleavage Activity ◽

Double Stranded Dna ◽

Dna Cleavage Activity ◽

Evolutionarily Conserved ◽

Toxin Protein ◽

Rna And Dna

AbstractType II toxin-antitoxin systems contain a toxin protein, which mediates diverse interactions within the bacterial cell when it is not bound by its cognate antitoxin protein. These toxins provide a rich source of evolutionarily-conserved tertiary folds that mediate diverse catalytic reactions. These properties make toxins of interest in biotechnology applications, and studies of the catalytic mechanisms continue to provide surprises. In the current work, our studies on a YoeB family toxin from Agrobacterium tumefaciens have revealed a conserved ribosome-independent non-specific nuclease activity. We have quantified the RNA and DNA cleavage activity, revealing they have essentially equivalent dose-dependence while differing in requirements for divalent cations and pH sensitivity. The DNA cleavage activity is as a nickase for any topology of double-stranded DNA, as well as cleaving single-stranded DNA. AtYoeB is able to bind to double-stranded DNA with mid-micromolar affinity. Comparison of the ribosome-dependent and -independent reactions demonstrates an approximate tenfold efficiency imparted by the ribosome. This demonstrates YoeB toxins can act as non-specific nucleases, cleaving both RNA and DNA, in the absence of being bound within the ribosome.

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Synthesis, characterization, antimicrobial, pesticidal and DNA cleavage activity of germanium(IV) derivatives of 3-(2-methyl-2,3-dihydro-benzthiazo-2-yl)-chromen-2-one and N′-[1-2-oxo-2H-chrome-3yl-ethylidene]-hydrazinecarbodithionic acid benzyl ester ligands

Main Group Metal Chemistry ◽

10.1515/mgmc-2012-0902 ◽

2011 ◽

Vol 34 (5-6) ◽

pp. 139-146 ◽

Cited By ~ 2

Author(s):

Latika Dawara ◽

Nighat Fahmi ◽

R.V. Singh

Keyword(s):

Antimicrobial Activity ◽

Metal Complexes ◽

Dna Cleavage ◽

Metal Ion ◽

Benzyl Ester ◽

Germanium Atom ◽

Conventional Heating ◽

Molar Ratio ◽

Cleavage Activity ◽

Dna Cleavage Activity

Abstract The new ligands 3-(2-methyl-2,3-dihydro-benzthiazo-2-yl)-chromen-2one (AcBzH) and N′-[1-2-oxo-2H-chrome-3yl-ethylidene]-hydrazinecarbodithionic acid benzyl ester (AcBDTZH) were prepared by the reaction of 3-acetyl-2H-chromen-2-one with 2-aminothiophenol and S-benzyl dithiocarbazate, respectively. The germanium(IV) complexes have been prepared by reacting Ph3GeCl and Me3GeCl in 1:1 molar ratio with these monofunctional bidentate AcBzH and AcBDTZH ligands by using microwave as well as conventional heating methods for comparison purposes. All the synthesized compounds were characterized by elemental analyses, melting point, IR, 1H-NMR 13C-NMR, mass and X-ray powder diffraction techniques. These studies showed that the ligands coordinated to the germanium atom in a monobasic bidentate manner and trigonal bipyramidal environment around the germanium atom have been established for the complexes. To evaluate the effect of metal ion upon chelation, both ligands and their complexes have been screened for their antimicrobial activity against the various pathogenic bacterial and fungal strains. The metal complexes have shown antimicrobial activity as compared to the free ligands. The pesticidal activity and DNA cleavage activity of both ligands and their metal complexes have been tested and discussed.

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Synthesis, characterization and DNA cleavage activity of nickel(II) adducts with aromatic heterocyclic bases

Journal of the Serbian Chemical Society ◽

10.2298/jsc1001061b ◽

2010 ◽

Vol 75 (1) ◽

pp. 61-74 ◽

Cited By ~ 5

Author(s):

Surendra Babu ◽

Pitchika Krishna ◽

Hussain Reddy ◽

G.H. Philip

Keyword(s):

Dna Cleavage ◽

Magnetic Moments ◽

Nuclease Activity ◽

Mixed Ligand ◽

Mixed Ligand Complexes ◽

Cleavage Activity ◽

Redox Couples ◽

Dna Cleavage Activity ◽

Calf Thymus ◽

Heterocyclic Bases

Mixed ligand complexes of nickel(II) with 2,4-dihydroxyacetophenone oxime (DAPO) and 2,4-dihydroxybenzophenone oxime (DBPO) as primary ligands, and pyridine (Py) and imidazole (Im) as secondary ligands were synthesized and characterized by molar conductivity, magnetic moments measurements, as well as by electronic, IR, and 1H-NMR spectroscopy. Electrochemical studies were performed by cyclic voltammetry. The active signals are assignable to the NiIII/II and NiII/I redox couples. The binding interactions between the metal complexes and calf thymus DNA were investigated by absorption and thermal denaturation. The cleavage activity of the complexes was determined using double-stranded pBR322 circular plasmid DNA by gel electrophoresis. All complexes showed increased nuclease activity in the presence of the oxidant H2O2. The nuclease activities of mixed ligand complexes were compared with those of the parent copper(II) complexes.

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Synthesis, Characterization of Some Transition-Metal Complexes of a New Heptadentate N5S2 Schiff-Base Ligand and the Effects of These Metal Complexes on U2OS Cells Cytotoxicity and DNA Cleavage Activity

Phosphorus Sulfur and Silicon and the Related Elements ◽

10.1080/10426500801907589 ◽

2008 ◽

Vol 183 (9) ◽

pp. 2237-2247 ◽

Cited By ~ 3

Author(s):

G. Uluçcam ◽

N. Beynek ◽

Z. Seller ◽

G. Akalın ◽

G. Turan ◽

...

Keyword(s):

Schiff Base ◽

Transition Metal ◽

Metal Complexes ◽

Transition Metal Complexes ◽

Schiff Base Ligand ◽

Dna Cleavage ◽

Cleavage Activity ◽

Dna Cleavage Activity ◽

U2os Cells

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Yb/chitosan catalyzed synthesis of highly substituted piperidine derivatives for potential nuclease activity and DNA binding study

Current Pharmaceutical Design ◽

10.2174/1381612826666201210114343 ◽

2020 ◽

Vol 26 ◽

Author(s):

Khairujjaman Laskar ◽

Mohd Farhan ◽

Aamir Ahmad

Keyword(s):

Dna Cleavage ◽

Nuclease Activity ◽

Substituted Anilines ◽

Cleavage Activity ◽

Dna Cleavage Activity ◽

Nano Catalyst ◽

Pbr322 Dna ◽

Catalyst Reusability ◽

The Stability ◽

First Time

Background: Herein, a new chitosan supported ytterbium nano-catalyst has been prepared and used for mild, efficient, and expeditious method for the synthesis of substituted piperidine derivatives via three component condensation of substituted anilines, formaldehyde and different cyclic/acyclic active methylene compounds at room temperature. Methods: The catalyst was characterized by FTIR, XRD, SEM, EDX, TEM, ICP-AES and the stability of catalyst was evaluated by TG analysis. The synthesized compound 3,3,11,11-Tetramethyl-15-(phenyl)-15- azadispiro[5.1.5.3]hexadecane-1,5,9,13-tetrone (3a) was explored for pBR322 DNA cleavage activity and genotoxicity for the first time. Further, the interaction study of 3a with CT-DNA was investigated through UV-vis, Fluorescence and Viscosity method. Results: The successful preparation of Yb/chitosan nano-catalyst was proved using various techniques and the catalyst was found effective towards substituted piperidine formations with the catalyst reusability. Further, compound 3a was successfully tested for DNA cleavage activity. In addition, Fluorescence results revealed that compound 3a interacts with DNA having a binding affinity of 4.84 x 104 M-1 . Conclusion: Hence, it could be suggested that compounds bearing spiro-piperidine scaffold synthesized using reusable nano-catalyst would be an effective biological agent.

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DNA Binding and Cleavage Activity of Binuclear Metal Complexes with Benzil-α-Monoxime Thiosemicarbzone

E-Journal of Chemistry ◽

10.1155/2011/567979 ◽

2011 ◽

Vol 8 (s1) ◽

pp. S309-S317

Author(s):

M. S. Surendra Babu ◽

T. B. Patrudu ◽

K. Hussain Reddy

Keyword(s):

Metal Complexes ◽

Dna Cleavage ◽

Magnetic Moments ◽

Nuclease Activity ◽

Molar Conductance ◽

Electrochemical Behaviors ◽

Voltammetric Studies ◽

Binuclear Metal ◽

Cyclic Voltammetric Studies ◽

Dna Binding And Cleavage

Transition metal complexes of copper(II), nickel(II), cobalt(II) and iron(II) with benzil-α-monoxime thiosemicarbazone (BMOT) have been synthesized and characterized by molar conductance, magnetic moments, IR, electronic and ESR spectroscopy. Electrochemical behaviors of these complexes were investigated by cyclic voltammetric studies. The nuclease activity of these complexes has been investigated on double-stranded pBR322 circular plasmid DNA by using the gel electrophoresis experiments in presence and absence of oxidant (H2O2). In the absence of oxidant DNA cleavage by hydrolytically was observed a less discernable, whereas in presence of oxidant (H2O2) all complexes showed increased nuclease activity.

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