scholarly journals Control of IntracellularFrancisella tularensisby Different Cell Types and the Role of Nitric Oxide

2014 ◽  
Vol 2014 ◽  
pp. 1-8 ◽  
Author(s):  
Sarah L. Newstead ◽  
Amanda J. Gates ◽  
M. Gillian Hartley ◽  
Caroline A. Rowland ◽  
E. Diane Williamson ◽  
...  
Keyword(s):  
Nitric Oxide ◽  
Cell Line ◽  
Rational Design ◽  
Cell Types ◽  
No Production ◽  
Macrophage Cell Line ◽  
Macrophage Cell ◽  
Schu S4 ◽  
Intracellular Infections

Reactive nitrogen is critical for the clearance ofFrancisella tularensisinfections. Here we assess the role of nitric oxide in control of intracellular infections in two murine macrophage cell lines of different provenance: the alveolar macrophage cell line, MH-S, and the widely used peritoneal macrophage cell line, J774A.1. Cells were infected with the highly virulent Schu S4 strain or with the avirulent live vaccine strain (LVS) with and without stimuli. Compared to MH-S cells, J774A.1 cells were unresponsive to stimulation and were able to control the intracellular replication of LVS bacteria, but not of Schu S4. In MH-S cells, Schu S4 demonstrated control over cellular NO production. Despite this, MH-S cells stimulated with LPS or LPS and IFN-γwere able to control intracellular Schu S4 numbers. However, only stimulation with LPS induced significant cellular NO production. Combined stimulation with LPS and IFN-γproduced a significant reduction in intracellular bacteria that occurred whether high levels of NO were produced or not, indicating that NO secretion is not the only defensive cellular mechanism operating in virulentFrancisellainfections. Understanding howF. tularensisinteracts with host macrophages will help in the rational design of new and effective therapies.

Role of nitric oxide in hydroxyapatite-induced phagocytosis by murine macrophage cell line (RAW264.7)

2006 ◽  
Vol 51 (4) ◽  
pp. 339-344 ◽  
Author(s):  
V.K. Gopinath ◽  
M. Musa ◽  
A.R. Samsudin ◽  
P. Lalitha ◽  
W. Sosroseno
Keyword(s):  
Nitric Oxide ◽  
Cell Line ◽  
Murine Macrophage ◽  
Macrophage Cell Line ◽  
Macrophage Cell ◽  
Murine Macrophage Cell Line

Enhanced Expression of Inducible Nitric Oxide Synthase by Juzen-Taiho-To in LPS-Activated RAW264.7 Cells, a Murine Macrophage Cell Line

2000 ◽  
Vol 28 (02) ◽  
pp. 217-226 ◽  
Author(s):  
Hiroshi Kawamata ◽  
Hiroshi Ochiai ◽  
Naoki Mantani ◽  
Katsutoshi Terasawa
Keyword(s):  
Nitric Oxide ◽  
Mrna Expression ◽  
Cell Line ◽  
Raw264.7 Cells ◽  
Inos Mrna ◽  
No Production ◽  
Murine Macrophage ◽  
Macrophage Cell Line ◽  
Macrophage Cell ◽  
Murine Macrophage Cell Line

We have investigated the effect of Juzen-taiho-to (TJ-48) on inducible NO synthase (iNOS) expression and nitric oxide (NO) production in RAW264.7 cells, a murine macrophage cell line. TJ-48-lipopolysaccharide (LPS) combination induced iNOS mRNA expression earlier, stronger and remained longer that paralleled but with a higher NO production compared to LPS stimulation. TJ-48 itself showed no inducible effect either no NO production or iNOS mRNA expression. This phenomenon could be considered to contribute, at least in part, to the beneficial effects of TJ-48 through the iNOS-mediated activation of biodefense mechanism.

Inducible Activity of Ginger Rhizome (Zingiber Offifinale Rosc.) on the mRNA Expression of Macrophage-Inducible Nitric Oxide (NO) Synthase and NO Production in a Macrophage Cell Line, RAW264.7 Cells

2004 ◽  
Vol 32 (05) ◽  
pp. 727-735 ◽  
Author(s):  
Nobuko Imanishi ◽  
Naoki Mantani ◽  
Shinya Sakai ◽  
Miyuki Sato ◽  
Yuko Katada ◽  
...  
Keyword(s):  
Nitric Oxide ◽  
Mrna Expression ◽  
Cell Line ◽  
Kinetic Studies ◽  
No Synthase ◽  
Raw264.7 Cells ◽  
No Production ◽  
Macrophage Cell Line ◽  
Macrophage Cell ◽  
Inducible Nitric Oxide

We have investigated the effect of Zingiber offifinale Rosc. (ZOR) on macrophage-inducible nitric oxide (NO) synthase (macNOS) mRNA expression and NO production in RAW264.7 cells, a murine macrophage cell line; 100 μg/ml ZOR can induce macNOS mRNA expression, but induction effects at a dose below 10 μg/ml were weak or negligible. Kinetic studies showed that macNOS mRNA can be detected from 4 hours to 24 hours after dosing, with a peak at 8 hours. In accordance with the induction of macNOS mRNA expression, NO concentrations increased from 3.4 μM at 2 hours to almost 150 μM at 24 hours, reflecting a longer period of macNOS mRNA expression. The activity of ZOR can be considered to contribute, at least in part, to the beneficial effects of ZOR through the macNOS-mediated activation of the biodefense mechanism.

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