scholarly journals Exogenous IL-4-Expressing Bone Marrow Mesenchymal Stem Cells for the Treatment of Autoimmune Sensorineural Hearing Loss in a Guinea Pig Model

2014 ◽  
Vol 2014 ◽  
pp. 1-10 ◽  
Author(s):  
Chang-qiang Tan ◽  
Xia Gao ◽  
Lang Guo ◽  
He Huang
Keyword(s):  
Stem Cells ◽  
Bone Marrow ◽  
Mesenchymal Stem Cells ◽  
Hearing Loss ◽  
Sensorineural Hearing Loss ◽  
Inner Ear ◽  
Sensorineural Hearing ◽  
Scala Tympani ◽  
Auditory Brain Stem Response ◽  
Marrow Mesenchymal Stem Cells

Bone marrow mesenchymal stem cells (BMSCs) expressing recombinant IL-4 have the potential to remediate inflammatory diseases. We thus investigated whether BMSCs expressing exogenous IL-4 could alleviate autoimmune sensorineural hearing loss. BMSCs isolated from guinea pigs were transfected with recombinant lentivirus expressing IL-4. A total of 33 animals were divided into three groups. Group A received scala tympani injection of IL-4-expressing BMSCs, and Group B received control vector-expressing BMSCs, and Group C received phosphate-buffered saline. The distribution of implanted BMSCs in the inner ears was assessed by immunohistochemistry and fluorescence microscopy. Auditory brain-stem response (ABR) was monitored to evaluate the auditory changes. Following BMSCs transplantation, the threshold levels of ABR wave III decreased in Groups A and B and significant differences were observed between these two groupsP<0.05. Transplanted BMSCs distributed in the scala tympani and scala vestibuli. In some ears with hearing loss, there was a decrease in the number of spiral ganglion cells and varying degrees of endolymphatic hydrops or floccule. Following transplantation, the lentivirus-infected BMSCs migrated to the inner ear and produced IL-4. Our results demonstrate that, upon transplantation, BMSCs and BMSCs expressing recombinant IL-4 have the ability to remediate the inflammatory injury in autoimmune inner ear diseases.

scholarly journals Therapeutic Efficacy of Bone Marrow Derived Mesenchymal Stem Cells in Ototoxic Sensorineural Hearing Loss

2020 ◽  
Vol 63 (12) ◽  
pp. 564-569
Author(s):  
Subin Kim ◽  
Yoon Hee Kwon ◽  
In Beom Kim ◽  
Young Jun Seo ◽  
Jae Sang Han ◽  
...  
Keyword(s):  
Stem Cells ◽  
Bone Marrow ◽  
Mesenchymal Stem Cells ◽  
Hearing Loss ◽  
Stem Cell ◽  
Sensorineural Hearing Loss ◽  
Sensorineural Hearing ◽  
Control Group ◽  
Clinical Grade ◽  
Brainstem Response

Background and Objectives Ototoxic sensorineural hearing loss causes permanent hearing loss in most cases. Recently there have been many reports describing cell base therapy with stem cells that has some effect on hearing recovery. We evaluated the efficacy of clinical grade, pre-made, human bone marrow derived mesenchymal stem cells (BM-MSCs) in ototoxic deaf animal model.Materials and Method BM-MSCs were cultured in a clinical grade laboratory. The animals were divided into 2 groups as follows: a saline injected control group and a stem cell injected group (MSC-group). Cultured MSCs were transplanted into the brachial vein of the deaf mice model. We recorded auditory brainstem response (ABR) and conducted immunohistochemistry at 1, 3, and 5 weeks.Results After the transplantation of MSC, a significant improvement in the hearing threshold of ABR was observed in the MSC transplanted group. Five weeks after transplantation of MSCs, hair cell regeneration was confirmed from the basal to the apex of the cochlea in fluorescent dyed image under the microscope compared to the control group.Conclusion BM-MSCs were effective in an acute ototoxic deaf animal model. These results show that stem cell transplantation mediate inner ear regeneration.

Inner ear progenitor cells can be generated in vitro from human bone marrow mesenchymal stem cells

2012 ◽  
Vol 7 (6) ◽  
pp. 757-767 ◽  
Author(s):  
Sarah L Boddy ◽  
Wei Chen ◽  
Ricardo Romero-Guevara ◽  
Lucksy Kottam ◽  
Illaria Bellantuono ◽  
...  
Keyword(s):  
Stem Cells ◽  
Bone Marrow ◽  
Mesenchymal Stem Cells ◽  
Inner Ear ◽  
Progenitor Cells ◽  
Human Bone ◽  
Human Bone Marrow ◽  
Marrow Mesenchymal Stem Cells

scholarly journals Bone marrow mesenchymal stem cells are progenitors in vitro for inner ear hair cells

2007 ◽  
Vol 34 (1) ◽  
pp. 59-68 ◽  
Author(s):  
Sang-Jun Jeon ◽  
Kazuo Oshima ◽  
Stefan Heller ◽  
Albert S.B. Edge
Keyword(s):  
Stem Cells ◽  
Bone Marrow ◽  
Mesenchymal Stem Cells ◽  
Inner Ear ◽  
Hair Cells ◽  
Marrow Mesenchymal Stem Cells

scholarly journals Mesenchymal stem cells for sensorineural hearing loss: protocol for a systematic review of preclinical studies

2019 ◽  
Vol 8 (1) ◽  
Author(s):  
Kevin T. Chorath ◽  
Matthew J. Willis ◽  
Nicolas Morton-Gonzaba ◽  
Walter J. Humann ◽  
Alvaro Moreira
Keyword(s):  
Systematic Review ◽  
Stem Cells ◽  
Mesenchymal Stem Cells ◽  
Hearing Loss ◽  
Sensorineural Hearing Loss ◽  
Sensorineural Hearing ◽  
Preclinical Studies

scholarly journals Audiological manifestations in patients of upper airway allergy

2019 ◽  
Vol 5 (6) ◽  
pp. 1451
Author(s):  
Gurbinder Singh Sekhon ◽  
Rohit Verma ◽  
Manish Munjal ◽  
Hemant Chopra
Keyword(s):  
Hearing Loss ◽  
Sensorineural Hearing Loss ◽  
Inner Ear ◽  
Otoacoustic Emissions ◽  
Upper Airway ◽  
Sensorineural Hearing ◽  
Study Group ◽  
Auditory Brain Stem Response ◽  
Distortion Product ◽  
Significant Difference

<p class="abstract"><strong>Background:</strong> The pathophysiology of involvement of inner ear in patients of upper airway allergy is poorly understood. The endolymphatic sac may be the likely seat of involvement in these patients as it can process antigens and produce its own local immune response. The aim of study was to assess the audiological profile of these patients.</p><p class="abstract"><strong>Methods:</strong> 53 patients of upper airway allergy (33 females and 20 males with mean age 25.77 years) and 20 control subjects (9 females and 11 males with mean age of 35.65 years) underwent haematological and audiological assessment.  </p><p class="abstract"><strong>Results:</strong> The study group had sensorineural hearing loss at 4000 and 8000 Hz frequencies. Abnormal distortion product otoacoustic emissions (DPOAEs) were noted in the study group as compared with the controls. On auditory brain stem response testing, no statistically significant difference was noted in the absolute latencies of waves I, III and V between study and control groups. Also no statistically significant difference was noted in the wave I-III and wave I-V inter peak latencies between the two groups.</p><p class="abstract"><strong>Conclusions:</strong> We found higher prevalence of high frequency sensorineural hearing loss and abnormalities of DPOAEs in patients having upper airway allergy. The likely seat of damage appears to be the inner ear as evidenced by abnormalities of DPOAEs. However the exact pathophysiology of inner ear damage in patients of upper airway allergy is poorly understood and needs further research.</p>

scholarly journals Mesenchymal stem cells for sensorineural hearing loss: a systematic review of preclinical studies

2020 ◽  
Vol 47 (6) ◽  
pp. 4723-4736 ◽  
Author(s):  
Kevin Chorath ◽  
Matthew Willis ◽  
Nicolas Morton-Gonzaba ◽  
Alvaro Moreira
Keyword(s):  
Systematic Review ◽  
Stem Cells ◽  
Mesenchymal Stem Cells ◽  
Hearing Loss ◽  
Sensorineural Hearing Loss ◽  
Sensorineural Hearing ◽  
Preclinical Studies

Role of Stem Cells in Treatment of Sensorineural Hearing Loss

QJM ◽  
2021 ◽  
Vol 114 (Supplement_1) ◽  
Author(s):  
Hesham El-Halaby ◽  
Ayman El-Kahky ◽  
Hesham Taha ◽  
Fatma Abu-Zahra ◽  
Ihab Nada ◽  
...  
Keyword(s):  
Stem Cells ◽  
Bone Marrow ◽  
Hearing Loss ◽  
Sensorineural Hearing Loss ◽  
Hair Cells ◽  
Guinea Pigs ◽  
Sensorineural Hearing ◽  
Group A ◽  
Group B

Abstract Background More than 5% of the world population lives with a hearing impairment. The main factors responsible for hearing degeneration are ototoxic drugs, aging, continued exposure to excessive noise and infections. The pool of adult stem cells in the inner ear drops dramatically after birth, and therefore an endogenous cellular source for regeneration is absent. Objectives The purpose of this study is to evaluate the role of mesenchymal stem cells in the treatment of severe to profound sensorineural hearing loss as regarding permanent regeneration of the auditory hair cells. Materials and Methods It is a prospective study which was performed in the period of May 2019 till December 2019 and encountered 40 adult male guinea pigs, aged 6 to 8 weeks (body weight 250-500 gram). 30 guinea pigs had been subjected to right side intratympanic injection of Garamycin sulphate to induce sensorineural hearing loss then the 30 animals were subdivided after that into 2 groups; Group A (15 animals) were treated by mesenchymal stem cells implantation in right inner ear cochlea and group B (15 animals) left untreated and the remaining 10 guinea pigs; Group C left normally as a control group. Results As respect to histopathological examination, 100% of animals in group B (n = 15) expressed severe degenerative changes in the peripheral organs. The outer hair cells showed severe destruction with individual loss of the inner hair cells. Also the supporting cells of Corti’s organ presented severe necrosis which was more relevant in the basal turns. We used the bone marrow differentiated MSCs to evaluate their potency in regeneration of the damaged and lost sensorineural elements in gentamicin injured cochlea. In group A (n = 15) 9 out of 15 (60%) expressed profound regeneration and 4 out of 15 (26.66%) expressed early regenerative changes, while 2 animals (13.33%) expressed less degenerative changes which impacted by gentamicin. Conclusion Stem cell transplantation is a promising approach for hearing loss therapy. The choice of stem cell type for transplantation plays a crucial role in the outcome. MSCs are multipotent cells that can be isolated from adult bone marrow and can be induced to differentiate into a variety of tissues in vitro and in vivo.

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