scholarly journals MALDI-TOF Mass Array Analysis ofNell-1Promoter Methylation Patterns in Human Gastric Cancer

2015 ◽  
Vol 2015 ◽  
pp. 1-8 ◽  
Author(s):  
Changlu Gao ◽  
Qian Zhang ◽  
Deyang Kong ◽  
Di Wu ◽  
Changlei Su ◽  
...  
Keyword(s):  
Gastric Cancer ◽  
Dna Methylation ◽  
Early Diagnosis ◽  
Normal Tissue ◽  
Methylation Status ◽  
Muscle Layer ◽  
Gastric Cancer Tissue ◽  
Cancer Tissue ◽  
Human Gastric Cancer ◽  
Significant Difference

Mass spectrometry (MS) enables rapid and sensitive qualitative and quantitative analyses of biomolecules (proteins, peptides, oligosaccharides, lipids, DNA, and RNA), drugs, and metabolites. MS has become an essential tool in modern biomedical research, including the analysis of DNA methylation. DNA methylation has been reported in many cancers, suggesting that it can be utilized as an early biomarker to improve the early diagnosis rate. Using matrix-assisted laser desorption/ionization time-of-flight MS and MassCLEAVE reagent, we comparedNell-1hypermethylation levels among tumor tissues, paracarcinoma tissues, and normal tissues from gastric cancer patients. Almost 80% of the CpG sites in the amplicons produced were covered by the analysis. Our results indicate a significant difference in methylation status between gastric cancer tissue (a higher level) and normal tissue. The same trend was identified in gastric cancer tissue versus paracarcinoma tissue. We also detected lower relative expression ofNell-1by real-time PCR. Furthermore, immunohistochemical analyses revealed thatNell-1staining was less intense in cancer tissue relative to normal tissue and that the tumor cells had spread to the muscle layer. These findings may serve as a guide for the early diagnosis of gastric cancer.

Comparison of iodine contents in gastric cancer and surrounding normal tissues

2005 ◽  
Vol 43 (6) ◽  
Author(s):  
Mine Gulaboglu ◽  
Leyla Yildiz ◽  
Fehmi Celebi ◽  
Mustafa Gul ◽  
Kemal Peker
Keyword(s):  
Gastric Cancer ◽  
Iodine Deficiency ◽  
Normal Tissue ◽  
Gastric Cancer Tissue ◽  
Cancer Tissue ◽  
Cancer Prevalence ◽  
Normal Tissues ◽  
The North ◽  
Significant Difference ◽  
North Eastern

AbstractIt has been suggested that iodine plays an important role in gastric cancer. Gastric cancer ranks first among the cancers in the north-eastern Anatolia region, Turkey, where iodine deficiency is common. In this study, iodine levels were determined in gastric cancer and surrounding normal tissues in 19 patients with gastric cancer. Tissue iodine levels were determined by the Foss method based on the Sandell-Kolt-hoff reaction. Tissue iodine levels were lower in gastric cancer tissue (17.8±3.4ngI/mg protein, mean±SEM) compared with surrounding normal tissue (41.7±8.0ngI/mg protein) (p<0.001). There was positive correlation between the iodine levels in gastric cancer tissue and surrounding normal tissue (r=0.845, p<0.001). There was no significant difference in iodine levels in cancer and surrounding normal tissue between male and female subjects. The iodine deficiency in our region may be one of the factors for increased gastric cancer prevalence. Our results support the hypothesis that iodine plays an important role in gastric cancer development.

scholarly journals Cell proliferation and apoptosis in gastric cancer and intestinal metaplasia

2005 ◽  
Vol 42 (1) ◽  
pp. 30-34 ◽  
Author(s):  
Nora Manoukian Forones ◽  
Ana Paula Souza Carvalho ◽  
Oswaldo Giannotti-Filho ◽  
Laércio Gomes Lourenço ◽  
Celina Tizuko Fujiyama Oshima
Keyword(s):  
Gastric Cancer ◽  
Intestinal Metaplasia ◽  
Stage Iv ◽  
Gastric Cancer Tissue ◽  
Cancer Tissue ◽  
Advanced Stage ◽  
Two Samples ◽  
Significant Difference ◽  
Proliferation And Apoptosis ◽  
Gastric Cancer Patients

BACKGROUND: Higher proliferation is commonly observed in cancer cells. Apoptosis can be a useful measure of a tumor cell kinetic. Alteration of the balance between proliferation and apoptosis is associated with cancer. AIM: To study proliferation and apoptosis on gastric cancer and in intestinal metaplasia. METHODOLOGY: Twenty-two samples from gastric adenocarcinomas and 22 biopsies from intestinal metaplasia were studied. The apoptotic bodies in hematoxylin-eosin slides and the expression of p53, bcl-2 and Ki67 were determined by immunohistochemistry. RESULTS: The number of the apoptotic cells was higher in cancer. Ki 67LI increased from intestinal metaplasia to gastric cancer. p53 was positive in 68% of the patients with cancer, more frequently in advanced stage and negative in samples of intestinal metaplasia. Although there was no significant difference between the groups, bcl-2 was positive in 45% of gastric cancer tissue and in 68% of metaplasia. In gastric cancer patients bcl-2 was expressed in early gastric cancer more frequently than in advanced stage. CONCLUSION: The positivity of bcl-2 was higher in metaplasia and probably is involved in the progression of carcinogenesis. p53 was negative in metaplasia and positive in more than half of the gastric cancer, mostly in stage IV, suggesting a late event in gastric cancer.

Effects of Bcl-2, Bax and P53 Gene Protein Expressions in Gastric Cancer Tissue on the Apoptosis of Cancer Cells

2013 ◽  
Vol 423-426 ◽  
pp. 358-361
Author(s):  
Lei Zhang ◽  
Da Peng Li ◽  
Guan Nan Lu
Keyword(s):  
Gastric Cancer ◽  
P53 Protein ◽  
P53 Gene ◽  
Gastric Cancer Tissue ◽  
Normal Lung ◽  
Cancer Tissue ◽  
P53 Expression ◽  
Bax Protein ◽  
Significant Difference ◽  
Positive Rate

Objective: To investigate the expression of p53 gene in gastric cancer tissue and its correlation with bcl-2 and bax protein expression in relationship and discuss the significance of their correlation in clinic. Methods: Pathological specimens from 100 gastric cancer patients with complete medical data and 24 normal lung tissue specimens were selected. Immunohistochemical streptavidin-biotin-peroxidase assay was used to detect the expression of bcl-2, bax and p53 protein. Results: p53 expression was positively correlated with bcl-2 expression (Pearson's R =0.491, P <0.05). The positive expression of p53 was no significantly correlated with bax expression (P> 0.05). The positive rate of p53 expression in the well-differentiated gastric cancer tissues was 17.6%, the positive rate in the differentiated tissues was 90.9%, the positive rate in the poorly differentiated tissues was 72.7%, and There was a significant difference in the expression of p53 in the three tissues (P <0.05). The expression of p53 in the TNM staging showed that the positive rate in stage Iand IIwas 27.3%, the positive rate in stage III and stage IVwas 89.3%, the difference between them was significant (P <0.05). Conclusions: The expression of p53 gene has an obvious clinical significance for the assessment of degree of malignancy and the prognosis of gastric cancer. Bcl-2 and p53 gene expression could jointly promote the development of gastric cancer in a synergistic way by acting on different stages of apoptosis, or bcl-2s participating in p53-induced mitochondria-mediated apoptosis signaling pathways.

scholarly journals Activation of c-Met (hepatocyte growth factor receptor) in human gastric cancer tissue

2004 ◽  
Vol 95 (10) ◽  
pp. 803-809 ◽  
Author(s):  
Takao Inoue ◽  
Hiroaki Kataoka ◽  
Kouichiro Goto ◽  
Koki Nagaike ◽  
Ko Igami ◽  
...  
Keyword(s):  
Gastric Cancer ◽  
Growth Factor ◽  
Hepatocyte Growth Factor ◽  
Growth Factor Receptor ◽  
Gastric Cancer Tissue ◽  
Cancer Tissue ◽  
Human Gastric Cancer ◽  
Hepatocyte Growth Factor Receptor ◽  
Hepatocyte Growth

scholarly journals Knockdown of Microtubule Associated Serine/threonine Kinase Like Expression Inhibits Gastric Cancer Cell Growth and Induces Apoptosis by Activation of ERK1/2 and Inactivation of NF-κB Signaling

2021 ◽  
Vol 41 (1) ◽  
pp. 108-117
Author(s):  
Cai-xia An ◽  
Shou-pin Xie ◽  
Hai-long Li ◽  
Yong-hua Hu ◽  
Rong Niu ◽  
...  
Keyword(s):  
Gastric Cancer ◽  
Cell Cycle ◽  
Tumor Cell ◽  
Tumor Cells ◽  
Normal Tissue ◽  
Gastric Cancer Tissue ◽  
Cancer Tissue ◽  
Adjacent Normal Tissue ◽  
Serine Threonine Kinase ◽  
Threonine Kinase

SummaryMicrotubule-associated serine/threonine kinase (MASTL) functions to regulate chromosome condensation and mitotic progression. Therefore, aberrant MASTL expression is commonly implicated in various human cancers. This study analyzed MASTL expression in gastric cancer vs. adjacent normal tissue for elucidating the association with clinicopathological data from patients. This work was then extended to investigate the effects of MASTL knockdown on tumor cells in vitro. The level of MASTL expression in gastric cancer tissue was assessed from the UALCAN, GEPIA, and Oncomine online databases. Lentivirus carrying MASTL or negative control shRNA was infected into gastric cancer cells. RT-qPCR, Western blotting, cell viability, cell counting, flow cytometric apoptosis and cell cycle, and colony formation assays were performed. MASTL was upregulated in gastric cancer tissue compared to the adjacent normal tissue, and the MASTL expression was associated with advanced tumor stage, Helicobacter pylori infection and histological subtypes. On the other hand, knockdown of MASTL expression significantly reduced tumor cell viability and proliferation, and arrested cell cycle at G2/M stage but promoted tumor cells to undergo apoptosis. At protein level, knockdown of MASTL expression enhanced levels of cleaved PARP1, cleaved caspase-3, Bax and p-ERK1/2 expression, but downregulated expression levels of BCL-2 and p-NF-κB-p65 protein in AGS and MGC-803 cells. MASTL overexpression in gastric cancer tissue may be associated with gastric cancer development and progression, whereas knockdown of MASTL expression reduces tumor cell proliferation and induces apoptosis. Further study will evaluate MASTL as a potential target of gastric cancer therapeutic strategy.

Comparative Immunofluorescence Analysis of Beta-III Tubulin (TUBB3) Expression in the Gastric Cancer Tissue and Morphologically Normal Tissue Adjacent to the Tumor

2021 ◽  
Vol 76 (2) ◽  
pp. 122-126
Author(s):  
T. A. Bogush ◽  
E. M. Kapura-Brekhovskikh ◽  
A. A. Basharina ◽  
E. A. Bogush ◽  
V. Yu. Kirsanov ◽  
...  
Keyword(s):  
Gastric Cancer ◽  
Normal Tissue ◽  
Gastric Cancer Tissue ◽  
Cancer Tissue ◽  
Immunofluorescence Analysis
Sign in / Sign up

Export Citation Format

Share Document