scholarly journals Supplementation ofCitrus maximaPeel Powder Prevented Oxidative Stress, Fibrosis, and Hepatic Damage in Carbon Tetrachloride (CCl4) Treated Rats

2015 ◽  
Vol 2015 ◽  
pp. 1-10 ◽  
Author(s):  
Mohammed Riaz Hasan Chowdhury ◽  
Md Abu Taher Sagor ◽  
Nabila Tabassum ◽  
Md Abdullah Potol ◽  
Hemayet Hossain ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Phenolic Compounds ◽  
Carbon Tetrachloride ◽  
Inflammatory Cells ◽  
Hepatoprotective Activity ◽  
Oxidation Products ◽  
Iron Deposition ◽  
Stress Markers ◽  
Citrus Maxima ◽  
Natural Phenolic Compounds

Citrus maximapeel is rich in natural phenolic compounds and has a long use in the traditional medicine. HPLC-DAD analysis onCitrus maximapeel powder exhibited the presence of various phenolic compounds such as caffeic acid and (−)-epicatechin. To determine the plausible hepatoprotective activity ofCitrus maximapeel powder, we used carbon tetrachloride (CCl4) treated rat model. Liver damage in rats was confirmed by measuring the AST, ALT, and ALP enzyme activities. In addition, lipid peroxidation products (MDA), nitric oxide, advanced protein oxidation products level (APOP), and catalase activities were also analyzed along with the histological profiling for the inflammatory cell infiltration, collagen, and iron deposition in liver. Dietary supplementation ofCitrus maximapeel powder exhibited significant reduction of serum AST, ALT, and ALP activities in carbon tetrachloride treated rats. Moreover,Citrus maximapeel powder also showed a significant reduction of the oxidative stress markers (MDA, NO, and APOP level) and restored the catalase activity in CCl4treated rats. Histological examination of the liver section revealed reduced inflammatory cells infiltration, collagen, and iron deposition in CCl4treated rats. The results from this study demonstrated thatCitrus maximapeel powder produced significant hepatoprotective action in CCl4administered rats.

EVALUATION OF HEPATO - PROTECTIVE ACTIVITY OF K-LIV DS ON CARBON TETRACHLORIDE INDUCED HEPATOTOXICITY IN RATS

INDIAN DRUGS ◽  
2018 ◽  
Vol 55 (08) ◽  
pp. 52-58
Author(s):  
D. Jain ◽  
◽  
K. Chainani
Keyword(s):  
Oxidative Stress ◽  
Alkaline Phosphatase ◽  
Carbon Tetrachloride ◽  
Histopathological Examination ◽  
Hepatoprotective Activity ◽  
Histopathological Study ◽  
Metabolic Function ◽  
Sleeping Time ◽  
Stress Markers ◽  
Ccl4 Administration

Present study investigates K-Liv DS, a polyherbal formulation on carbon tetrachloride induced hepatotoxicity in Wistar rats. Rats were divided into normal control, CCl4 control, CCl4+K-Liv DS (0.5 ml) and CCl4+K-LivDS (1 mL). CCl4 was injected intraperitoneally from day 1 to day 10, while K Liv DS was administered orally for 14 days. Hepatoprotective activity was assessed by estimating transaminases, alkaline phosphatase, total protein and bilirubin in serum. Estimation of oxidative stress markers and histopathological study of liver were also carried out. Metabolic function of liver was evaluated by thiopentone induced sleeping time. CCl4 administration produced a significant hepatotoxicity (P<0.001), which was reversed with K-Liv DS treatment. It significantly decreased transaminases, alkaline phosphatase and bilirubin and increased total proteins (P<0.001). Moreover oxidative stress was decreased and metabolic function was improved by decrease in sleeping time. Histopathological examination revealed no abnormalities in the K-Liv DS treated rats. K-Liv DS is an effective hepatoprotective agent and has potential clinical applications for liver diseases.

scholarly journals Pharmacoprophylaxis of liver diseases: creating a new hepatoprotector

2020 ◽  
Vol 17 ◽  
pp. 00061
Author(s):  
Svetlana Zykova ◽  
Sergey Shurov ◽  
Aleksey Savinkov ◽  
Nino Gugushvili ◽  
Vladimir Talismanov
Keyword(s):  
Oxidative Stress ◽  
Carbon Tetrachloride ◽  
Toxic Effect ◽  
Alanine Aminotransferase ◽  
Liver Diseases ◽  
Hepatoprotective Activity ◽  
Control Group ◽  
Biochemical Indicators ◽  
Intact Group ◽  
Biochemical Studies

The article presents a study of the hepatoprotective activity of a tricyclic heterocycle, which refers to 5, 6, 7, 8-tetrahydroquinolines. The effect of 8, 8-dimethyl-5-p-tolyl-8, 9-dihydro-2H-pyrido [4, 3, 2-de] cinnolin-3 (7H) was studied on rats under the influence of the model of toxic hepatosis induced by carbon tetrachloride to find out the indicators of peroxidation and biochemical indicators. Biochemical studies have shown that modelling toxic fat hepatosis caused by the inception of carbon tetrachloride to rats increased the activity of alanine aminotransferase by 2.5 times more compared with the intact group, indicating the development of oxidative stress induced by the treatment of pyrido [4, 3, 2] Cinnol I that reduced the toxic effect of CTC by 79.9 %. Mexidol had a less pronounced hepatoprotective effect: the activity of Alanine aminotransferase on animals of the second group was lower by 29.2 % than on rats from the control group. Thus, a new compound with hepatoprotective activity has been developed and studied.

scholarly journals Xanthine Oxidase Inhibitor, Allopurinol, Prevented Oxidative Stress, Fibrosis, and Myocardial Damage in Isoproterenol Induced Aged Rats

2015 ◽  
Vol 2015 ◽  
pp. 1-9 ◽  
Author(s):  
Md. Abu Taher Sagor ◽  
Nabila Tabassum ◽  
Md. Abdullah Potol ◽  
Md. Ashraful Alam
Keyword(s):  
Oxidative Stress ◽  
Myocardial Infarction ◽  
Inflammatory Cells ◽  
Oxidase Inhibitor ◽  
Oxidative Stress Markers ◽  
Aged Rats ◽  
Sample Collection ◽  
Xanthine Oxidase Inhibitor ◽  
Glutathione Concentration ◽  
Stress Markers

We evaluated the preventive effect of allopurinol on isoproterenol (ISO) induced myocardial infarction in aged rats. Twelve- to fourteen-month-old male Long Evans rats were divided into three groups: control, ISO, and ISO + allopurinol. At the end of the study, all rats were sacrificed for blood and organ sample collection to evaluate biochemical parameters and oxidative stress markers analyses. Histopathological examinations were also conducted to assess inflammatory cell infiltration and fibrosis in heart and kidneys. Our investigation revealed that the levels of oxidative stress markers were significantly increased while the level of cellular antioxidants, catalase activity, and glutathione concentration in ISO induced rats decreased. Treatment with allopurinol to ISO induced rats prevented the elevated activities of AST, ALT, and ALP enzymes, and the levels of lipid peroxidation products and increased reduced glutathione concentration. ISO induced rats also showed massive inflammatory cells infiltration and fibrosis in heart and kidneys. Furthermore, allopurinol treatment prevented the inflammatory cells infiltration and fibrosis in ISO induced rats. In conclusion, the results of our study suggest that allopurinol treatment is capable of protecting heart of ISO induced myocardial infarction in rats probably by preventing oxidative stress, inflammation, and fibrosis.

In Vitroandin VivoAntioxidant Activity of Alfalfa (Medicago sativaL.) on Carbon Tetrachloride Intoxicated Rats

2012 ◽  
Vol 40 (04) ◽  
pp. 779-793 ◽  
Author(s):  
Mohammed S. Al-Dosari
Keyword(s):  
Oxidative Stress ◽  
Carbon Tetrachloride ◽  
Lipid Profile ◽  
Histopathological Examination ◽  
Serum Levels ◽  
Hepatoprotective Activity ◽  
Test Substance ◽  
Protein Sulfhydryl ◽  
Serum Transaminases

The present study was conducted to determine whether lyophilized aqueous extract of alfalfa, or Medicago sativa L. could exert antioxidant activity against carbon tetrachloride-induced oxidative stress and liver injury in rats. The hepatoprotective activity of alfalfa extract was determined by assessing the levels of serum transaminases, ALP, bilirubin and lipid profile. Further, the effect of the test substance on malondialdehyde (MDA), an end product of lipid peroxidation; antioxidant liver enzyme non-protein sulfhydryl (NP-SH); and total protein (TP) were also studied. Serum transaminase, ALP, bilirubin level, lipid profile and liver MDA were significantly elevated and the antioxidant status in liver NP-SH and TP contents were declined in animals treated with CCl4alone. Pretreatment with alfalfa and silymarin for three weeks prior to the administration of CCl4significantly prevented the increase in the serum levels of hepatic marker, LDL, VLDL levels enzymes and reduced oxidative stress indicated by elevated NP-SH and TP concentration. The histopathological examination of the livers also showed that the alfalfa extract reduced the incidence of liver lesions induced by CCl4. The in vitro antioxidant assessment of alfalfa extract on DPPH and carotene-linoleic assays demonstrated a moderate antioxidant potential. Results suggest that the alfalfa extract possesses hepatoprotective and antioxidative stress properties possibly through its antioxidant phytochemical constituents and substantiates its use in various liver disorders as a hepatoprotector.

scholarly journals Antifibrotic Activity of Phaleria macrocarpa Extract in Rat Liver-fibrosis Model: Focus on Oxidative Stress Markers, TGF-β1 and MMP-13

2020 ◽  
Vol 8 (A) ◽  
pp. 555-562
Author(s):  
Bantari W. K. Wardhani ◽  
Nanik Sundari ◽  
Raymond R. Tjandrawinata ◽  
Ahmad Aulia Jusuf ◽  
Vivian Soetikno ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Carbon Tetrachloride ◽  
Liver Fibrosis ◽  
Liver Function ◽  
Cell Activation ◽  
Stellate Cell ◽  
Male Rats ◽  
Oxidative Stress Markers ◽  
Stress Markers ◽  
Phaleria Macrocarpa

AIM: This study was aimed to determine the antifibrotic activity of Phaleria macrocarpa (PM) extract in liver fibrosis (LF) and its possible mechanism in the rat model. METHODS: Sprague Dawley male rats were injected with 2 mL/kg BW of carbon tetrachloride intraperitoneally twice a week for 2 weeks, followed by 1 mL/kg BW for 6 weeks. Afterward, the treatments began from the 3rd week: Silymarin 100 mg/kg BW/day, standardized PM extract (Proliverenol) 75 or 150 mg/kg BW/day orally. Rats were sacrificed in the 8th week. Blood and liver were collected to analyze liver function, liver damage and fibrosis marker, oxidative stress markers, pro-fibrogenic cytokine, and antifibrotic marker. RESULTS: Our study showed that the treatment of silymarin and PM resulted in the normalized activity of liver function, followed by the amelioration of oxidative stress, demonstrated by the decreased malondialdehyde levels and an increased ratio of glutathione and glutathione disulfide. All markers examined showed that PM extract has antioxidant activity due to decreased hepatic stellate cell activation. We also found a decrease in tumor growth factors-β1 and protein expressions of matrix metalloproteinases-13 in all treatment groups compared to the carbon tetrachloride group. There were tendencies of the decreased fibrotic area following improvements of biochemical parameters. CONCLUSION: PM extracts ameliorate carbon tetrachloride-induced LF. The proposed mechanism is by overcoming oxidative stress and regulating pro-fibrogenic cytokine and antifibrotic markers.

scholarly journals Experimental Evaluation of Prophylactic and Therapeutic Antioxidant Potential of Trimetazidine in Carbon Tetrachloride Induced Oxidative Stress in Rats

2021 ◽  
Author(s):  
Vijay Haribhau Mate ◽  
Vijaya Anil Pandit ◽  
Pradnya Hemant Padalkar ◽  
Chetan Shrirang More ◽  
Kapil S Khade
Keyword(s):  
Oxidative Stress ◽  
Antioxidant Activity ◽  
Carbon Tetrachloride ◽  
Treated Group ◽  
Hepatoprotective Activity ◽  
Antioxidant Potential ◽  
Oxidative Stress Marker ◽  
Control Group ◽  
Group Iii ◽  
Group I

Introduction: Exposure to various drugs and chemicals lead to oxidative stress. Carbon Tetrachloride (CCl4) produces rise in oxidative stress leading to hepatic damage. The drug Trimetazidine (TMZ) shows hepatoprotective activity but its mechanism is not known. The present study would help in establishing antioxidant activity of TMZ as probable mechanism. Aim: To evaluate the antioxidant potential of TMZ in CCl4 induced oxidative stress when given prophylactically/therapeutically in rats. Materials and Methods: An experimental animal study was conducted on 80 adult Wistar rats of either sex (weight-150 to 200 gm) from March 2010 to December 2010 in Bharati Vidyapeeth Medical College, Pune, Maharashtra, India. Randomly, all animals were grouped into 10 equal groups. Group i was normal control (received only water). To induce oxidative stress CCl4 (0.5 mL/kg/d i.p.) was given to all the animals of Group ii to Group x for seven days. The TMZ was given in two doses, TMZ1 (5 mg/kg orally for Group iii and vii) and TMZ2 (10 mg/kg orally for Group iv and viii). Positive standard control (Group v and Group ix) received Liv.52 (1 mL/kg orally). Group vi and Group x received combination of TMZ1 (5 mg/kg orally)+Liv.52 (1 mL/kg orally). Drug treatment was given to animals in group iii, iv, v and vi for 1-14 days (preventive group) and in group vii, viii, ix and x from day 8 to day 14 (therapeutic group). On 15th day, rats were sacrificed and dissected for collection of liver. Part of the livers was homogenised to assess oxidative stress marker enzymes Malondialdehyde (MDA), Superoxide Dismutase (SOD) spectrophotometrically. Statistical analysis was done with one- way Analysis of Variance (ANOVA) followed by post-hoc analysis (Dunnett’s test) using GraphPad Prism 5.0 software. Results: Trimetazidine (5 mg/kg and 10 mg/kg) significantly reduced MDA levels and increased SOD levels when compared with CCl4 treated group suggested antioxidant activity. Combined administration of Liv.52 and TMZ1 also reduced oxidative stress and increased antioxidant activity. Conclusion: Results of the present study suggested that increased oxidative stress was significantly attenuated by drug TMZ in dose dependant manner when compared with the CCl4 group. The antioxidant potential of prophylactic and therapeutic administration of TMZ was comparable. The increased antioxidant effect by Liv.52+TMZ1 combination was only due to the additive antioxidant effects of Liv.52 and TMZ or any other mechanism was involved, needs to be further evaluated.

The Protective Effect of the Ethanol Leaf Extract of Andrographis Paniculata on Cisplatin-Induced Acute Kidney Injury in Rats Through nrf2/KIM-1 Signalling Pathway

Drug Research ◽  
2017 ◽  
Vol 68 (01) ◽  
pp. 23-32 ◽  
Author(s):  
Bisi Adeoye ◽  
Ebunoluwa Asenuga ◽  
Ademola Oyagbemi ◽  
Temidayo Omobowale ◽  
Adeolu Adedapo
Keyword(s):  
Oxidative Stress ◽  
Normal Saline ◽  
Leaf Extract ◽  
Kidney Injury ◽  
Inflammatory Cells ◽  
Andrographis Paniculata ◽  
Stress Markers ◽  
Markers Of Oxidative Stress ◽  
Group B ◽  
Lower Expression

AbstractThe ethanol leaf extract of Andrographis paniculata was used to ameliorate the renal toxicity induced by cisplatin in 28 rats divided into four groups of seven rats per group. Group A received normal saline for the duration of the experiment. Group B animals were treated with cisplatin (10 mg/kg i.p) on day 1 and 3 days after received normal saline for the next 7 days while groups C and D animals also received 10 mg/kg dose of cisplatin on day 1 but after 3 days were then respectively treated with 200 and 400 mg/kg doses of the extract of Andrographis paniculata for the remaining 7 days through oral administration. Serum chemistry was used for the determination of markers of oxidative stress, anti-oxidant enzymes, serum biomarkers etc. Histopathology and immunohistochemistry were also carried out. Results showed that all oxidative stress markers assayed were significantly increased in group B animals but reverse is the case for groups C and D. On the other hand, antioxidant enzymes assayed experienced significant increase for groups C and D while these parameters experienced significant decrease for group B animals. Histopathology showed severe infiltration of inflammatory cells into renal tissues of group B animals whereas for groups C and D animals, only moderate glomerular degeneration was noted. In immunohistochemistry, while there is higher expression of KIM-1 for group B, there was a lower expression in groups C and D. Again, there was lower expression of Nrf2 for group B but higher expressions in groups C and D animals.

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