scholarly journals Antimicrobial Activity Evaluation on Silver Doped Hydroxyapatite/Polydimethylsiloxane Composite Layer

2015 ◽  
Vol 2015 ◽  
pp. 1-13 ◽  
Author(s):  
C. S. Ciobanu ◽  
A. Groza ◽  
S. L. Iconaru ◽  
C. L. Popa ◽  
P. Chapon ◽  
...  
Keyword(s):  
Antimicrobial Activity ◽  
Candida Albicans ◽  
Photoelectron Spectroscopy ◽  
Laser Scanning ◽  
Composite Layer ◽  
Physicochemical Characterization ◽  
Antimicrobial Effect ◽  
Laser Scanning Microscopy ◽  
Confocal Laser ◽  
Composite Layers

The goal of this study was the preparation, physicochemical characterization, and microbiological evaluation of novel hydroxyapatite doped with silver/polydimethylsiloxane (Ag:HAp-PDMS) composite layers. In the first stage, the deposition of polydimethylsiloxane (PDMS) polymer layer on commercially pure Si disks has been produced in atmospheric pressure corona discharges. Finally, the new silver doped hydroxyapatite/polydimethylsiloxane composite layer has been obtained by the thermal evaporation technique. The Ag:HAp-PDMS composite layers were characterized by various techniques, such as Scanning Electron Microscopy (SEM), Glow Discharge Optical Emission Spectroscopy (GDOES), and X-ray photoelectron spectroscopy (XPS). The antimicrobial activity of the Ag:HAp-PDMS composite layer was assessed againstCandida albicansATCC 10231 (ATCC—American Type Culture Collection) by culture based and confirmed by SEM and Confocal Laser Scanning Microscopy (CLSM) methods. This is the first study reporting the antimicrobial effect of the Ag:HAp-PDMS composite layer, which proved to be active againstCandida albicansbiofilm embedded cells.

scholarly journals Bioceramic Layers with Antifungal Properties

Coatings ◽  
2018 ◽  
Vol 8 (8) ◽  
pp. 276 ◽  
Author(s):  
Daniela Predoi ◽  
Simona Iconaru ◽  
Mihai Predoi
Keyword(s):  
Candida Albicans ◽  
Antifungal Activity ◽  
Laser Scanning ◽  
Sol Gel ◽  
Laser Scanning Microscopy ◽  
Confocal Laser ◽  
X Ray Diffraction ◽  
Composite Layers ◽  
Constituent Elements ◽  
Microbial Strain

The sol-gel method was used to synthesize the silver doped hydroxyapatite (Ag:HAp) gels in order to produce the antifungal composite layers. The pure Ti disks were used as the substrate for the composite layers. Important information about suspensions used to make Ag:HAp composite layers were obtained from an ultrasonic technique. The identification of the phase composition of the Ag:HAp composite layers was accomplished X-ray diffraction (XRD). The morphology and the thickness of the layers was evaluated using scanning electron microscopy (SEM). The uniform distribution of the constituent elements (Ag, Ca, P, and O) in both analyzed samples was observed. The antifungal activity of the samples against Candida albicans ATCC 10231 microbial strain were investigated immediately after their preparation and six months later. SEM and confocal laser scanning microscopy (CLSM) images showed that the composite layers at the two time intervals exhibited a strong antifungal activity against Candida albicans ATCC 10231 and completely inhibited the biofilm formation.

scholarly journals Antimicrobial Effect of Photodynamic Therapy Using Plaque Disclosing Agent

2020 ◽  
Vol 47 (2) ◽  
pp. 120-127
Author(s):  
Junhee Kho ◽  
Howon Park ◽  
Juhyun Lee ◽  
Hyunwoo Seo ◽  
Siyoung Lee
Keyword(s):  
Candida Albicans ◽  
Photodynamic Therapy ◽  
Lactobacillus Casei ◽  
Laser Scanning ◽  
Light Emitting Diode ◽  
Antimicrobial Effect ◽  
Laser Scanning Microscopy ◽  
Confocal Laser ◽  
Light Emitting ◽  
Colony Forming Unit

The aim of this study was to evaluate the antimicrobial effect of photodynamic therapy (PDT) using plaque disclosing agent, 10 - 20 mM erythrosine, as a photosensitizer.<br/>Multispecies cariogenic biofilms containing Streptococcus mutans , Lactobacillus casei and Candida albicans were formed on hydroxyapatite disc. 20 μM, 10 mM and 20 mM erythrosine were applied as a photosensitizer for 3 minutes, and then light-emitting diode (LED) irradiated for 24 seconds. Colony-forming unit (CFU) were measured and biofilms were observed using confocal laser scanning microscopy (CLSM). CFU were significantly decreased in the PDT groups using 10 - 20 mM erythrosine (10 mM, 20mM) and the results were also confirmed by CLSM.<br/>This study confirms the high antimicrobial effect of photodynamic therapy using plaque disclosing agent as a photosensitizer.

scholarly journals Investigation of Spin Coating Cerium-Doped Hydroxyapatite Thin Films with Antifungal Properties

Coatings ◽  
2021 ◽  
Vol 11 (4) ◽  
pp. 464
Author(s):  
Simona Liliana Iconaru ◽  
Mihai Valentin Predoi ◽  
Patrick Chapon ◽  
Sofia Gaiaschi ◽  
Krzysztof Rokosz ◽  
...  
Keyword(s):  
Electron Microscopy ◽  
Scanning Electron Microscopy ◽  
Photoelectron Spectroscopy ◽  
Spin Coating ◽  
Laser Scanning ◽  
Laser Scanning Microscopy ◽  
Confocal Laser ◽  
X Ray ◽  
Antifungal Properties ◽  
Scanning Electron

In this study, the cerium-doped hydroxyapatite (Ca10−xCex(PO4)6(OH)2 with xCe = 0.1, 10Ce-HAp) coatings obtained by the spin coating method were presented for the first time. The stability of the 10Ce-HAp suspension particles used in the preparation of coatings was evaluated by ultrasonic studies, transmission electron microscopy (TEM), X-ray diffraction (XRD), and scanning electron microscopy (SEM). The surface morphology of the 10Ce-HAp coating was studied by SEM and atomic force microscopy (AFM) techniques. The obtained 10Ce-HAp coatings were uniform and without cracks or unevenness. Glow discharge optical emission spectroscopy (GDOES) and X-ray photoelectron spectroscopy (XPS) were used for the investigation of fine chemical depth profiling. The antifungal properties of the HAp and 10Ce-HAp suspensions and coatings were assessed using Candida albicans ATCC 10231 (C. albicans) fungal strain. The quantitative antifungal assays demonstrated that both 10Ce-HAp suspensions and coatings exhibited strong antifungal properties and that they successfully inhibited the development and adherence of C. albicans fungal cells for all the tested time intervals. The scanning electron microscopy (SEM) and confocal laser scanning microscopy (CLSM) visualization of the C. albicans fungal cells adherence to the 10Ce-HAp surface also demonstrated their strong inhibitory effects. In addition, the qualitative assays also suggested that the 10Ce-HAp coatings successfully stopped the biofilm formation.

scholarly journals Polymeric Nanoparticles Active against Dual-Species Bacterial Biofilms

Molecules ◽  
2021 ◽  
Vol 26 (16) ◽  
pp. 4958
Author(s):  
Jessa Marie V. Makabenta ◽  
Jungmi Park ◽  
Cheng-Hsuan Li ◽  
Aritra Nath Chattopadhyay ◽  
Ahmed Nabawy ◽  
...  
Keyword(s):  
Antimicrobial Activity ◽  
Laser Scanning ◽  
Polymeric Nanoparticles ◽  
Bacterial Species ◽  
Laser Scanning Microscopy ◽  
Confocal Laser ◽  
Bacterial Biofilms ◽  
Global Public Health ◽  
Bacterial Strains

Biofilm infections are a global public health threat, necessitating new treatment strategies. Biofilm formation also contributes to the development and spread of multidrug-resistant (MDR) bacterial strains. Biofilm-associated chronic infections typically involve colonization by more than one bacterial species. The co-existence of multiple species of bacteria in biofilms exacerbates therapeutic challenges and can render traditional antibiotics ineffective. Polymeric nanoparticles offer alternative antimicrobial approaches to antibiotics, owing to their tunable physico-chemical properties. Here, we report the efficacy of poly(oxanorborneneimide) (PONI)-based antimicrobial polymeric nanoparticles (PNPs) against multi-species bacterial biofilms. PNPs showed good dual-species biofilm penetration profiles as confirmed by confocal laser scanning microscopy. Broad-spectrum antimicrobial activity was observed, with reduction in both bacterial viability and overall biofilm mass. Further, PNPs displayed minimal fibroblast toxicity and high antimicrobial activity in an in vitro co-culture model comprising fibroblast cells and dual-species biofilms of Escherichia coli and Pseudomonas aeruginosa. This study highlights a potential clinical application of the presented polymeric platform.

scholarly journals Antibody response to the 45 kDa Candida albicans antigen in an animal model and potential role of the antigen in adherence

2008 ◽  
Vol 57 (12) ◽  
pp. 1466-1472 ◽  
Author(s):  
Helena Bujdáková ◽  
Ema Paulovičová ◽  
Silvia Borecká-Melkusová ◽  
Juraj Gašperík ◽  
Soňa Kucharíková ◽  
...  
Keyword(s):  
Animal Model ◽  
Candida Albicans ◽  
Biofilm Formation ◽  
Laser Scanning ◽  
Vaccine Development ◽  
Laser Scanning Microscopy ◽  
Model Systems ◽  
Confocal Laser

The Candida antigen CR3-RP (complement receptor 3-related protein) is supposed to be a ‘mimicry’ protein because of its ability to bind antibody directed against the α subunit of the mammalian CR3 (CD11b/CD18). This study aimed to (i) investigate the specific humoral isotypic response to immunization with CR3-RP in vivo in a rabbit animal model, and (ii) determine the role of CR3-RP in the adherence of Candida albicans in vitro using the model systems of buccal epithelial cells (BECs) and biofilm formation. The synthetic C. albicans peptide DINGGGATLPQ corresponding to 11 amino-acids of the CR3-RP sequence DINGGGATLPQALXQITGVIT, determined by N-terminal sequencing, was used for immunization of rabbits to obtain polyclonal anti-CR3-PR serum and for subsequent characterization of the humoral isotypic response of rabbits. A significant increase of IgG, IgA and IgM anti-CR3-RP specific antibodies was observed after the third (P<0.01) and the fourth (P<0.001) immunization doses. The elevation of IgA levels suggested peptide immunomodulation of the IgA1 subclass, presumably in coincidence with Candida epithelial adherence. Blocking CR3-RP with polyclonal anti-CR3-RP serum reduced the ability of Candida to adhere to BECs, in comparison with the control, by up to 35 % (P<0.001), and reduced biofilm formation by 28 % (P<0.001), including changes in biofilm thickness and integrity detected by confocal laser scanning microscopy. These properties of CR3-RP suggest that it has potential for future vaccine development.

scholarly journals Enamel Based Composite Layers Deposited on Titanium Substrate with Antifungal Activity

2016 ◽  
Vol 2016 ◽  
pp. 1-13 ◽  
Author(s):  
S. L. Iconaru ◽  
A. M. Prodan ◽  
C. S. Turculet ◽  
M. Beuran ◽  
R. V. Ghita ◽  
...  
Keyword(s):  
Antifungal Activity ◽  
Laser Scanning ◽  
Titanium Substrate ◽  
Significant Inhibition ◽  
Laser Scanning Microscopy ◽  
Confocal Laser ◽  
Composite Layers ◽  
Ethidium Bromide Staining ◽  
Biofilm Development ◽  
Promising Perspective

The goal of this paper was to investigate the antifungal activity of Enamel layers deposited on titanium substrate (Ti-Enamel) and Enamel layers deposited on titanium substrate previously coated with a vinyl polydimethylsiloxane layer (Ti-PDMS-Enamel). The physicochemical properties were also investigated. TheCandida albicansbiofilm development on the obtained layers was examined after 24 h, 48 h, and 72 h by confocal laser scanning microscopy (CLSM) and scanning electron microscopy (SEM) after ethidium bromide staining. A significant inhibition of the fungal adherence and biofilm development was observed on Ti-Enamel layers. The antifungal results demonstrated that the use of new Ti-Enamel composite layers could represent a promising perspective for the prevention of fungal biofilms associated implant infections.

Spectral characterisation of new organic fluorescent dyes with an alkoxysilane moiety and their utilisation for the labelling of layered silicates

2013 ◽  
Vol 67 (1) ◽  
Author(s):  
Martin Danko ◽  
Matej Mičušík ◽  
Mária Omastová ◽  
Juraj Bujdák ◽  
Dušan Chorvát
Keyword(s):  
Dicarboxylic Acid ◽  
Photoelectron Spectroscopy ◽  
Laser Scanning ◽  
Fluorescent Dyes ◽  
Stokes Shift ◽  
Particle Surface ◽  
Acid Anhydride ◽  
Laser Scanning Microscopy ◽  
Layered Silicates ◽  
Confocal Laser

AbstractNew fluorescence dyes with an alkoxysilane moiety were synthesised by the condensation of 3-(triethoxysilyl)-1-propanamine (3-aminopropyltriethoxysilane) with 4,10-benzothioxanthene-3,1′-dicarboxylic acid anhydride (BTXA) and N,N-dimethylaminonaphthalene-1,8-dicarboxylic acid anhydride (DMANA), which was accompanied by the formation of an imidic bridge. The compounds N-(3-(triethoxysilyl)propyl)-thioxantheno[2,1,9-dej]isoquinoline-1,3-dione (BTX-S) and 4-(N′, N′-dimethyl)-N-(triethoxysilyl)propyl-1,8-naphthalene dicarboxylic acid imide (DMAN-S) were characterised by steady-state and time-resolved fluorescence spectroscopy in chloroform and ethanol. Both conjugates (BTX-S and DMAN-S) exhibited absorption and emission bands in the same region as the un-substituted BTXA and DMANA. An important Stokes shift was observed for DMAN-S in ethanol. A high fluorescence quantum yield was observed for BTX-S in both solvents and for DMAN-S in chloroform. In addition, the newly developed fluorescent silane dyes were covalently attached to the microscopic particles of layered silicates and on the surface of SiO2 wafers as a proof of concept for fluorescence particle (surface) visualisation. The surface wafer modification was precisely characterised by X-ray photoelectron spectroscopy (XPS). Successful covalent linkage onto the particles of layered silicates was proved by confocal laser scanning microscopy technique.

scholarly journals A Novel Hybrid Peptide Composed of LfcinB6 and KR-12-a4 With Enhanced Antimicrobial, Anti-Inflammatory and Anti-Biofilm Activities

2021 ◽  
Author(s):  
Chelladurai Ajish ◽  
Sungtae Yang ◽  
S. Dinesh Kumar ◽  
Eun Young Kim ◽  
Hye Jung Min ◽  
...  
Keyword(s):  
Antimicrobial Activity ◽  
Laser Scanning ◽  
Antimicrobial Agents ◽  
Laser Scanning Microscopy ◽  
Confocal Laser ◽  
Bacterial Cells ◽  
Bacterial Strains ◽  
Hybrid Peptide ◽  
Cell Selectivity ◽  
Anti Inflammatory

Abstract Hybridizing two known antimicrobial peptides (AMPs) is a simple and effective strategy for designing antimicrobial agents with enhanced cell selectivity against bacterial cells. Here, we generated a hybrid peptide Lf-KR in which LfcinB6 and KR-12-a4 were linked with a Pro hinge to obtain a novel AMP with potent antimicrobial, anti-inflammatory, and anti-biofilm activities. Lf-KR exerted superior cell selectivity for bacterial cells over sheep red blood cells. Lf-KR showed broad-spectrum antimicrobial activities (MIC: 4–8 mM) against tested 12 bacterial strains and retained its antimicrobial activity in the presence of salts at physiological concentrations. Membrane depolarization and dye leakage assays showed that the enhanced antimicrobial activity of Lf-KR was due to increased permeabilization and depolarization of microbial membranes. Lf-KR significantly inhibited the expression and production of pro-inflammatory cytokines (NO and TNF-a) in LPS-stimulated mouse macrophage RAW264.7 cells. In addition, Lf-KR showed a powerful eradication effect on preformed multidrug-resistant Pseudomonas aeruginosa (MDRPA) biofilms. We confirmed using confocal laser scanning microscopy that a large portion of the preformed MDRPA biofilm structure was perturbed by the addition of Lf-KR. Collectively, our results suggest that Lf-KR can be an antimicrobial, anti-inflammatory, and anti-biofilm candidate as a pharmaceutical agent.

scholarly journals Electrochemical and surface analytical techniques applied to microbiologically influenced corrosion investigation

2018 ◽  
Vol 36 (4) ◽  
pp. 349-363 ◽  
Author(s):  
László Trif ◽  
Abdul Shaban ◽  
Judit Telegdi
Keyword(s):  
Photoelectron Spectroscopy ◽  
Laser Scanning ◽  
Electrochemical Impedance ◽  
Electrochemical Techniques ◽  
Analytical Techniques ◽  
Laser Scanning Microscopy ◽  
Microbiologically Influenced Corrosion ◽  
Microbial Consortia ◽  
Confocal Laser ◽  
The Impact

AbstractSuitable application of techniques for detection and monitoring of microbiologically influenced corrosion (MIC) is crucial for understanding the mechanisms of the interactions and for selecting inhibition and control approaches. This paper presents a review of the application of electrochemical and surface analytical techniques in studying the MIC process of metals and their alloys. Conventional electrochemical techniques, such as corrosion potential (Ecorr), redox potential, dual-cell technique, polarization curves, electrochemical impedance spectroscopy (EIS), electrochemical noise (EN) analysis, and microelectrode techniques, are discussed, with examples of their use in various MIC studies. Electrochemical quartz crystal microbalance, which is newly used in MIC study, is also discussed. Microscopic techniques [scanning electron microscopy (SEM), environmental SEM (ESEM), atomic force microscopy (AFM), confocal laser microscopy (CLM), confocal laser scanning microscopy (CLSM), confocal Raman microscopy] and spectroscopic analytical methods [Fourier transform infrared spectroscopy (FTIR) and X-ray photoelectron spectroscopy (XPS)] are also highlighted. This review highlights the heterogeneous characteristics of microbial consortia and use of special techniques to study their probable effects on the metal substrata. The aim of this review is to motivate using a combination of new procedures for research and practical measurement and calculation of the impact of MIC and biofilms on metals and their alloys.

Sign in / Sign up

Export Citation Format

Share Document