scholarly journals Cortex phellodendriExtract Relaxes Airway Smooth Muscle

2016 ◽  
Vol 2016 ◽  
pp. 1-9 ◽  
Author(s):  
Qiu-Ju Jiang ◽  
Weiwei Chen ◽  
Hong Dan ◽  
Li Tan ◽  
He Zhu ◽  
...  
Keyword(s):  
Smooth Muscle ◽  
Airway Smooth Muscle ◽  
Active Ingredient ◽  
Butyl Alcohol ◽  
Alcohol Extract ◽  
New Drug ◽  
High K ◽  
Cortex Phellodendri ◽  
Selective Blocker ◽  
Orai Channels

Cortex phellodendriis used to reduce fever and remove dampness and toxin. Berberine is an active ingredient ofC. phellodendri. Berberine fromArgemone ochroleucacan relax airway smooth muscle (ASM); however, whether the nonberberine component ofC. phellodendrihas similar relaxant action was unclear. An n-butyl alcohol extract ofC. phellodendri(NBAECP, nonberberine component) was prepared, which completely inhibits high K+- and acetylcholine- (ACH-) induced precontraction of airway smooth muscle in tracheal rings and lung slices from control and asthmatic mice, respectively. The contraction induced by high K+was also blocked by nifedipine, a selective blocker of L-type Ca2+channels. The ACH-induced contraction was partially inhibited by nifedipine and pyrazole 3, an inhibitor of TRPC3 and STIM/Orai channels. Taken together, our data demonstrate that NBAECP can relax ASM by inhibiting L-type Ca2+channels and TRPC3 and/or STIM/Orai channels, suggesting that NBAECP could be developed to a new drug for relieving bronchospasm.

Low-temperature Modification of the Inhibitory Effects of Volatile Anesthetics on Airway Smooth Muscle Contraction in Dogs

2000 ◽  
Vol 93 (1) ◽  
pp. 179-188 ◽  
Author(s):  
Michiaki Yamakage ◽  
Naoki Tsujiguchi ◽  
Jun-ichi Hattori ◽  
Yasuhiro Kamada ◽  
Akiyoshi Namiki
Keyword(s):  
Smooth Muscle ◽  
Low Temperature ◽  
Muscle Contraction ◽  
Airway Smooth Muscle ◽  
Volatile Anesthetics ◽  
Smooth Muscle Contraction ◽  
Channel Activity ◽  
Inhibitory Effects ◽  
High K ◽  
Voltage Dependent

Background Because exposure to low temperature can modify the effect of volatile anesthetics on airway smooth muscle contraction, this study was conducted to investigate low-temperature modifications of the inhibitory effects of isoflurane and sevoflurane on canine tracheal smooth muscle tone by simultaneously measuring the muscle tension and intracellular concentration of Ca2+ ([Ca2+]i) and by measuring voltage-dependent Ca2+ channel activity. Methods [Ca2+]i was monitored by the 500-nm light emission ratio of preloaded fura-2, a Ca2+ indicator. Isometric tension was measured simultaneously. Whole cell patch clamp recording techniques were used to observe voltage-dependent Ca2+ channel activity in dispersed muscle cells. Isoflurane (0-3.0%) or sevoflurane (0-3%) was introduced to a bath solution at various temperatures (37, 34, or 31 degrees C). Results Low temperature (34 or 31 degrees C) reduced high-K+-induced (72.7 mm) muscle contraction and increased [Ca2+]i, but it enhanced carbachol-induced (1 microm) muscle contraction with a decrease in [Ca2+]i. The volatile anesthetics tested showed significant inhibition of both high-K+-induced and carbachol-induced airway smooth muscle contraction, with a concomitant decrease in [Ca2+]i. The inhibition of the carbachol-induced muscle contraction by volatile anesthetics was abolished partially by exposure to low temperature. Volatile anesthetics and low-temperature exposure significantly inhibited voltage-dependent Ca2+ channel activity of the smooth muscle. Conclusions Exposure of airway smooth muscle to low temperature leads to an increase in agonist-induced muscle contractility, with a decrease in [Ca2+]i. The inhibition of voltage-dependent Ca2+ channel activity by exposure to low temperature and by volatile anesthetics cam be attributed, at least in part, to the decrease in [Ca2+]i.

scholarly journals Relaxant Action ofPlumula NelumbinisExtract on Mouse Airway Smooth Muscle

2015 ◽  
Vol 2015 ◽  
pp. 1-10 ◽  
Author(s):  
Li Tan ◽  
Weiwei Chen ◽  
Ming-Yu Wei ◽  
Jinhua Shen ◽  
Meng-Fei Yu ◽  
...  
Keyword(s):  
Smooth Muscle ◽  
Airway Smooth Muscle ◽  
Biological Activities ◽  
Chloroform Extract ◽  
Dependent Manner ◽  
Concentration Dependent Manner ◽  
Resting Tension ◽  
High K ◽  
Voltage Dependent ◽  
Anti Inflammation

The traditional herbPlumula Nelumbinisis widely used in the world because it has many biological activities, such as anti-inflammation, antioxidant, antihypertension, and butyrylcholinesterase inhibition. However, the action ofPlumula Nelumbinison airway smooth muscle (ASM) relaxation has not been investigated. A chloroform extract ofPlumula Nelumbinis(CEPN) was prepared, which completely inhibited precontraction induced by high K+in a concentration-dependent manner in mouse tracheal rings, but it had no effect on resting tension. CEPN also blocked voltage-dependent L-type Ca2+channel- (VDCC-) mediated currents. In addition, ACh-induced precontraction was also completely blocked by CEPN and partially inhibited by nifedipine or pyrazole 3. Besides, CEPN partially reduced ACh-activated nonselective cation channel (NSCC) currents. Taken together, our data demonstrate that CEPN blocked VDCC and NSCC to inhibit Ca2+influx, resulting in relaxation of precontracted ASM. This finding indicates that CEPN would be a candidate of new potent bronchodilators.

scholarly journals Coptisine, a protoberberine alkaloid, relaxes mouse airway smooth muscle via blockade of VDLCCs and NSCCs

2020 ◽  
Vol 40 (2) ◽  
Author(s):  
Nana Wen ◽  
Lu Xue ◽  
Yongle Yang ◽  
Shunbo Shi ◽  
Qing-Hua Liu ◽  
...  
Keyword(s):  
Smooth Muscle ◽  
Muscle Contraction ◽  
Airway Smooth Muscle ◽  
Molecular Mechanisms ◽  
Force Measurement ◽  
Dependent Manner ◽  
Active Constituent ◽  
High K ◽  
Voltage Dependent

Abstract Background/Aims: Recently, effective and purified ingredients of traditional Chinese medicine (TCM) were extracted to play crucial roles in the treatment of pulmonary diseases. Our previous research focused on TCM drug screening aimed at abnormal airway muscle contraction during respiratory diseases. Coptisine, an effective ingredient extracted from bitter herbs has shown a series of antioxidant, antibacterial, cardioprotective and neuroprotective pharmacological properties. In the current study, we questioned whether coptisine could also participate in asthma treatment through relaxing abnormal contracted mouse airway smooth muscle (ASM). The present study aimed to characterize the relaxant effects of coptisine on mouse ASM and uncover the underlying molecular mechanisms. Methods: To investigate the role of coptisine on pre-contracted mouse ASM, a series of biological techniques, including force measurement and patch-clamp experiments were employed. Results: Coptisine was found to inhibit high K+ or acetylcholine chloride (ACh)-induced pre-contracted mouse tracheal rings in a dose-dependent manner. Further research demonstrated that the coptisine-induced mouse ASM relaxation was mediated by alteration of calcium mobilization via voltage-dependent L-type Ca2+ channels (VDLCCs) and non-selective cation channels (NSCCs). Conclusion: Our data showed that mouse ASM could be relaxed by coptisine via altering the intracellular Ca2+ concentration through blocking VDLCCs and NSCCs, which suggested that this pharmacological active constituent might be classified as a potential new drug for the treatment of abnormal airway muscle contraction.

scholarly journals Cortex Phellodendri Extract Relaxes Mouse Airway Smooth Muscle

2016 ◽  
Vol 110 (3) ◽  
pp. 261a
Author(s):  
Weiwei Chen ◽  
Qui-Ju Jiang ◽  
Meng-Fei Yu ◽  
Qing-Hua Liu
Keyword(s):  
Smooth Muscle ◽  
Airway Smooth Muscle ◽  
Cortex Phellodendri

Electron Probe Analysis of Vertebrate Smooth Muscle: Distribution of Ca and Ci

1976 ◽  
Vol 34 ◽  
pp. 334-335 ◽  
Author(s):  
Avril V. Somlyo ◽  
H. Shuman ◽  
A.P. Somlyo
Keyword(s):  
Smooth Muscle ◽  
Electron Probe ◽  
Preliminary Report ◽  
Cell Damage ◽  
Electron Probe Analysis ◽  
Perinuclear Space ◽  
Probe Analysis ◽  
High K ◽  
Low K ◽  
Initial Results

This is a preliminary report of electron probe analysis of rabbit portal-anterior mesenteric vein (PAMV) smooth muscle cryosectioned without fixation or cryoprotection. The instrumentation and method of electron probe quantitation used (1) and our initial results with cardiac (2) and skeletal (3) muscle have been presented elsewhere.In preparations depolarized with high K (K2SO4) solution, significant calcium peaks were detected over the sarcoplasmic reticulum (Fig 1 and 2) and the continuous perinuclear space. In some of the fibers there were also significant (up to 200 mM/kg dry wt) calcium peaks over the mitochondria. However, in smooth muscle that was not depolarized, high mitochondrial Ca was found in fibers that also contained elevated Na and low K (Fig 3). Therefore, the possibility that these Ca-loaded mitochondria are indicative of cell damage remains to be ruled out.

Sign in / Sign up

Export Citation Format

Share Document