scholarly journals Cytotoxicity and Apoptosis Induction of Ardisia crispa and Its Solvent Partitions against Mus musculus Mammary Carcinoma Cell Line (4T1)

2017 ◽  
Vol 2017 ◽  
pp. 1-10 ◽  
Author(s):  
Muhammad Luqman Nordin ◽  
Arifah Abdul Kadir ◽  
Zainul Amiruddin Zakaria ◽  
Fauziah Othman ◽  
Rasedee Abdullah ◽  
...  
Keyword(s):  
Ethyl Acetate ◽  
Cell Line ◽  
Aqueous Extract ◽  
Mus Musculus ◽  
Mammary Carcinoma ◽  
Methanol Extract ◽  
Carcinoma Cell ◽  
Annexin V ◽  
Mammary Carcinoma Cell ◽  
4T1 Cells

This study was conducted to investigate the cytotoxicity and apoptosis effect of A. crispa extract and its solvent partition (ethyl acetate and aqueous extract) against Mus musculus mammary carcinoma cell line (4T1). The normal mouse fibroblast cell line (NIH3T3) was used as comparison for selective cytotoxicity properties. The cytotoxicity evaluation was assessed using MTT assay. AO/PI dual fluorescent staining assay and Annexin V-FITC were used for apoptosis analysis. Results showed that 80% methanol extract from leaves showed most promising antimammary cancer agent with IC50 value of 42.26±1.82 μg/mL and selective index (SI) value of 10.22. Ethyl acetate was cytotoxic for both cancer and normal cell while aqueous extract exhibited poor cytotoxic effect. 4T1 cells labelled with AO/PI and Annexin V-FITC and treated with 80% methanol extract demonstrated that the extract induces apoptosis to 4T1 mammary cancer cells. In conclusion, 80% methanol extract of A. crispa was selectively cytotoxic towards 4T1 cells but less cytotoxic towards NIH3T3 cells and induced the cancerous cells into apoptotic stage as early as 6 hours.

scholarly journals Immunotherapeutic efficacy of a Lactobacillus casei lysate as an adjuvant combined with a heated-4T1 mammary carcinoma cell lysate in a murine model of breast cancer

2017 ◽  
Vol 10 (4) ◽  
pp. 327-334 ◽  
Author(s):  
Ruhollah Dorostkar ◽  
Mohammad Sadegh Hashemzadeh ◽  
Sajjad Jafari ◽  
Mahdi Tat ◽  
Majdedin Ghalavand ◽  
...  
Keyword(s):  
Breast Cancer ◽  
Murine Model ◽  
Mammary Carcinoma ◽  
Tumor Size ◽  
Lactobacillus Casei ◽  
Tumor Antigens ◽  
Carcinoma Cell ◽  
Mammary Carcinoma Cell ◽  
Cell Lysate ◽  
4T1 Cells

Abstract Background Immunotherapy, during which the immune system of the patient is manipulated to act against tumors has been among the most successful methods in the treatment of breast cancer, a leading cause of mortality among women worldwide. Objectives To investigate the immunotherapeutic efficacy of Lactobacillus casei lysate as an adjuvant in combination with a heated-4T1 mammary carcinoma cell lysate in a model of breast cancer. Methods After ethics committee approval of all animal procedures, a murine model of breast cancer was induced in BALB/c mice using 4T1 cells. These mice were immunized with a combination of lysates of heated 4T1 cells and L. casei. Subsequent changes in tumor size and weight, and the production of TNF-α, IL-2, IL-12, IL-17, and IL13 were measured. Lung weights were measured as an indicator of metastasis to other organs. Results The tumor size and weight in mice immunized with the combined vaccine were significantly reduced compared with controls. The combined immunotherapy altered the pattern of cytokine production to the advantage of antitumor immunity, and was significantly more potent than immunization with heated-4T1-cell lysate or L. casei lysate alone. Conclusions Coadministration of L. casei lysate enhanced the immunotherapeutic efficacy of the heated-4T1-cell lysate as a source of tumor-associated antigens. L. casei can potentially be used as an adjuvant combined with sources of tumor antigens in the treatment of cancers, and as a safe alternative to the current adjuvants that cause greater irritation to hosts. Further studies are required to clarify the mechanisms underlying these effects.

Establishment of an apoptosis-sensitive rat mammary carcinoma cell line with a mutation in the DNA-binding region of p53

2006 ◽  
Vol 232 (2) ◽  
pp. 279-288 ◽  
Author(s):  
Tetsuya Hamaguchi ◽  
Yoichiro Matsuoka ◽  
John Bechberger ◽  
Takamasa Ohnishi ◽  
Ken-ichi Fujita ◽  
...  
Keyword(s):  
Dna Binding ◽  
Cell Line ◽  
Mammary Carcinoma ◽  
Carcinoma Cell ◽  
Carcinoma Cell Line ◽  
Mammary Carcinoma Cell ◽  
Binding Region ◽  
Mammary Carcinoma Cell Line

Effect of flavonone hesperidin on the apoptosis of human mammary carcinoma cell line MCF-7

2011 ◽  
Vol 1 (3) ◽  
pp. 207-215 ◽  
Author(s):  
Nandakumar Natarajan ◽  
Rengarajan Thamaraiselvan ◽  
Haribabu Lingaiah ◽  
Perumal Srinivasan ◽  
Balasubramanian Maruthaiveeran Periyasamy
Keyword(s):  
Cell Line ◽  
Mammary Carcinoma ◽  
Carcinoma Cell ◽  
Carcinoma Cell Line ◽  
Mammary Carcinoma Cell ◽  
Human Mammary Carcinoma ◽  
Mammary Carcinoma Cell Line ◽  
Human Mammary Carcinoma Cell ◽  
Mcf 7
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