scholarly journals Cardiometabolic Changes in Different Gonadal Female States Caused by Mild Hyperuricemia and Exposure to a High-Fructose Diet

2018 ◽  
Vol 2018 ◽  
pp. 1-9 ◽  
Author(s):  
J. Soutelo ◽  
Y. A. Samaniego ◽  
M. C. Fornari ◽  
C. Reyes Toso ◽  
O. J. Ponzo
Keyword(s):  
Uric Acid ◽  
Lipid Profile ◽  
Wistar Rats ◽  
Female Rats ◽  
Treatment Control ◽  
High Fructose Diet ◽  
High Fructose ◽  
Metabolic Systems ◽  
Female Wistar Rats ◽  
Oxonic Acid

Background. The objective of this study is to observe if mild hyperuricemia and a high-fructose diet influence the cardiovascular and metabolic systems in hypogonadic female Wistar rats compared to normogonadic female rats. Methods. Fifty-six (56) adult female Wistar rats were used in the present work. Animals were divided into two groups: normogonadic (NGN) and hypogonadic (HGN). These groups were also divided into four subgroups in accordance with the treatment: control with only water (C), fructose (F), oxonic acid (OA), and fructose + oxonic acid (FOA). Lipid profile, glycemia, uric acid, and creatinine determinations were assessed. Cardiovascular changes were evaluated by measuring blood pressure, myocyte volume, fibrosis, and intima-media aortic thickness. Results. HGN rats had higher levels of total cholesterol (TC) (p<0.01) and noHDLc (p<0.01), in addition to higher levels of uric acid (p<0.05). The OA group significantly increased myocyte volume (p<0.0001) and the percentage of fibrosis as well as the group receiving FOA (p<0.001) in both gonadal conditions, being greater in the HGN group. Hypogonadic animals presented a worse lipid profile. Conclusion. Mild hyperuricemia produces hypertension together with changes in the cardiac hypertrophy, fibrosis, and increased thickness of the intima media in hypogonadic rats fed high-fructose diet.

scholarly journals Investigation of the effect of coffee on body weight, serum glucose, uric acid and lipid profile levels in male albino Wistar rats feeding on high-fructose diet

2019 ◽  
Vol 35 (1) ◽  
Author(s):  
Teka Obsa Feyisa ◽  
Daniel Seifu Melka ◽  
Menakath Menon ◽  
Wajana Lako Labisso ◽  
Mezgebu Legesse Habte
Keyword(s):  
Metabolic Syndrome ◽  
Body Weight ◽  
Uric Acid ◽  
Lipid Profile ◽  
Wistar Rats ◽  
Serum Glucose ◽  
Control Group ◽  
Fasting Serum ◽  
High Fructose Diet ◽  
High Fructose

AbstractCoffee is one of the most commonly consumed beverages in the worldwide and is assumed to have protective effects against metabolic syndrome. The present study was aimed at investigating the effect of coffee on body weight, serum glucose, uric acid and lipid profile levels in male albino Wistar rats feeding on high fructose diet. A post-test experimental study was conducted on a total of 30 (9–10 weeks old) male albino Wistar rats. The rats were divided into 6 groups: group I (normal control)-fed on standard chow and plain tap water only; group II (fructose control)-fed on standard chow and 20% of fructose solution; group III–VI (treatment groups)-fed on standard chow, 20% of fructose solution and treated with 71, 142, 213 and 284 mg/kg body weight/day of coffee respectively for six weeks. At the end, body weight, serum glucose, uric acid and lipid profile levels were investigated. Data was entered and cleared by epi-data software version 3.1 and analyzed by one way ANOVA followed by Tukey post hoc multiple comparison tests using SPSS V. 23.00. Statistical significance was considered at p < 0.05. The results showed that body weight, fasting serum glucose and uric acid levels significantly lowered in rats treated with 213 (p = 0.047; 0.049; 0.026) and 284 (p = 0.035; 0.029; 0.010) mg/kg body weight/day of coffee compared to fructose control group. Fasting serum triglycide (TG) and low density lipoprotein (LDL-C) levels showed significant reduction in rats treated with 284 mg/kg body weight/day of coffee as compared to fructose control group (p = 0.031; 0.046) respectively. In conclusion, treating rats with coffee decreased body weight, fasting serum glucose, uric acid, TC, TG and LDL-C, and increased HDL-C in a dose dependent manner in rats feeding on high fructose diet, suggesting that coffee consumption may be helpful in ameliorating metabolic syndrome.

scholarly journals Influence of Normo- and Hypogonadal Condition, Hyperuricemia, and High-Fructose Diet on Renal Changes in Male Rats

2017 ◽  
Vol 2017 ◽  
pp. 1-8 ◽  
Author(s):  
Jimena Soutelo ◽  
Yanina Alejandra Samaniego ◽  
Elsa Zotta ◽  
María Cecilia Fornari ◽  
Carlos Reyes Toso ◽  
...  
Keyword(s):  
Gender Disparity ◽  
Male Rats ◽  
Renal Lesion ◽  
Treatment Control ◽  
High Fructose Diet ◽  
High Fructose ◽  
Renal Changes ◽  
Male Wistar Rats ◽  
Progression Of Renal Disease ◽  
Oxonic Acid

Background. There is a gender disparity in the incidence, prevalence, and progression of renal disease. The object of this paper is to evaluate the presence and type of renal lesion in normogonadic and hypogonadic male rats in a mild hyperuricemia induced condition and exposed to a high-fructose diet. Methods. 56 adult male Wistar rats were used. Animals were divided into two groups, one normogonadic (NGN) and one hypogonadic (HGN), and each group was divided into four subgroups in accordance with the treatment: control with only water (C), fructose (F), oxonic acid (OA), and fructose + oxonic acid (FOA). Renal changes were evaluated by measuring glomerulosclerosis, fibrosis, and arteriolar media/lumen (M/L) ratio.Results. The OA and FOA groups presented significantly hypertension (p<0.001). The OA group significantly increased (p<0.05) the percentage of glomerulosclerosis as well as the FOA group (p<0.001). When comparing NGN versus HGN, we observed a trend to a lower glomerulosclerosis in the latter. A higher arteriolar M/L ratio was observed in the OA (p<0.05) and FOA (p<0.001). Conclusion. Hyperuricemia conditions and a high-fructose diet favor blood pressure increase together with changes in the arteriolar media/lumen ratio and renal glomerular damage. These changes were more apparent in normogonadic animals.

scholarly journals INVESTIGATION OF THE EFFECTS OF ALLOXAN-INDUCED DIABETES ON REPRODUCTIVE HORMONES (FOLLICLE STIMULATING, LUTEINIZING AND PROLACTIN), LIPID PROFILE AND SERUM ELECTROLYTES IN MALE AND FEMALE WISTAR RATS

2020 ◽  
Vol 7 ◽  
pp. 40
Author(s):  
Bukonla Oluyemisi Adegbesan ◽  
Olugbenga Owolabi Ogunlabi ◽  
Temtope Abigail Fasanya ◽  
Ayodeji Adebayo Adelegan ◽  
Emmanuel Oladipupo Ajani
Keyword(s):  
Lipid Profile ◽  
Wistar Rats ◽  
Reproductive Hormones ◽  
Female Rats ◽  
Male And Female ◽  
Male And Female Rats ◽  
Type1 Diabetes ◽  
Female Wistar Rats ◽  
The Impact ◽  
Female Subjects

Human patients with Type1 diabetes and inadequate metabolic control have been found to exhibit a high prevalence of infertility but the exact mechanism by which this happens has not been fully elucidated. Alterations in the levels of lipids and electrolytes are considered to be good indications of some certain diseases including diabetes. This study aims at elucidating the impact of diabetes on the reproductive functions of male and female subjects as well as the responses of lipid profile which could be a determining factor affecting diabetic condition, to Alloxan-induced diabetes using male and female Wistar rats. To explore this, various hormonal assays assessing reproductive hormones such as Follicle stimulating hormone (FSH); Luteinizing hormone (LH); Prolactin hormone (PRL); lipid profile analyses and electrolytes measurements were employed. Our results showed that diabetes induce significant reduction in the levels of FSH and LH in both male and female rats (p<0.0001); significant reduction in the levels of LH in male (p<0.01) and female (p<0.0001); significant increase in the levels of prolactin (PRL) in male (p<0.05) and female (p<0.0001) ; significant increases in cholesterol levels in both male and female rats (p<0.01), an indication that diabetes is accompanied in most cases by hypercholesterolemia and triglycerides levels in both male (p<0.01) and female (p<0.0005) rats when compared to the control rats. The results obtained in this study shed more light on the mechanism by which fertility in male and female subjects is affected by diabetes and suggest possible mechanisms by which infertility could result from diabetes.

scholarly journals EFFECTS OF RESVERATROL ON LIVER FUNCTION OF OBESE FEMALE WISTAR RATS

2016 ◽  
Vol 17 (3) ◽  
pp. 402-410 ◽  
Author(s):  
Nádia Araújo Miguel ◽  
Silvia Franco Andrade ◽  
Gisele Nai ◽  
Cecília Braga Laposy ◽  
Felipe Franco Nascimento ◽  
...  
Keyword(s):  
Liver Function ◽  
Lipid Profile ◽  
Wistar Rats ◽  
Liver Enzymes ◽  
Obese Group ◽  
Female Rats ◽  
Control Group ◽  
Obese Female ◽  
Short Period ◽  
Female Wistar Rats

Abstract Resveratrol has antioxidant, anti-inflammatory, lipolytic, and antifibrotic properties, which may be useful in supplementation of obese patients and with liver problems. This study evaluated the effects of 6-week resveratrol supplementation on the lipid profile and liver function of female Wistar rats fed a high-fat diet to induce obesity. Sixty-four Wistar rats were divided into 4 groups (n = 16): the control group (C); the control obese group (CO); the resveratrol group (R); and the resveratrol obese group (RO). At the end of the experiment, the animals were anesthetized for blood collection and subsequent euthanasia for collection of liver biopsy. The parameters for body weight, liver weight, retroperitoneal fat weight, serum lipid and liver profiles and histopathological analysis were evaluated. The 6-week resveratrol administration did not induce weight loss nor did it reduce the lipid profile; however, it decreased the liver enzymes aspartate aminotransferase (AST) and alkaline phosphatase (ALP) and reduced the incidence of steatosis (75.0%) in group RO compared with group CO (81.2%). Thus, we concluded that resveratrol supplementation for the short period of six weeks had a beneficial effect on liver function by reducing hepatic steatosis and the liver enzymes AST and ALP in obese female rats.

NEW DATA CONCERNING THE ROLE PLAYED BY PROGESTERONE IN THE CONTROL OF FOLLICULAR GROWTH IN THE RAT

1974 ◽  
Vol 75 (3) ◽  
pp. 569-578 ◽  
Author(s):  
G. Buffler ◽  
S. Roser
Keyword(s):  
Wistar Rats ◽  
Venous Blood ◽  
Oestrous Cycle ◽  
Female Rats ◽  
Cycle Duration ◽  
Follicular Growth ◽  
The Third ◽  
Female Wistar Rats

ABSTRACT The mechanisms involved in the prolongation of the oestrous cycle following LH administration were studied in 4-day cyclic female Wistar rats. In females injected with LH on the morning of dioestrus I there was an increase in ovarian venous blood progesterone as compared with non-injected animals. In both LH-treated females, and those injected with progesterone on the morning of dioestrus I, a slowing up in follicular growth was observed from the afternoon of dioestrus I. The size of follicles greater than 400 urn present in LH or progesterone injected animals on the third day of cycle was similar to the size reached by the same range of follicles in non-injected animals on the second day of the cycle. Hence, the increase in endogenous ovarian progesterone elicited by LH was considered as the cause of the slowing up of follicular growth and therefore of the lengthening of the oestrous cycle duration in female rats injected with LH at the beginning of 4-day cycle.

scholarly journals High-Fructose Diet Increases Inflammatory Cytokines and Alters Gut Microbiota Composition in Rats

2020 ◽  
Vol 2020 ◽  
pp. 1-10 ◽  
Author(s):  
Yong Wang ◽  
Wentao Qi ◽  
Ge Song ◽  
Shaojie Pang ◽  
Zhenzhen Peng ◽  
...  
Keyword(s):  
Uric Acid ◽  
Inflammatory Response ◽  
Gut Microbiota ◽  
Inflammatory Cytokines ◽  
Proinflammatory Cytokines ◽  
Lipid Accumulation ◽  
Fasting Blood Glucose ◽  
High Fructose Diet ◽  
Fructose Intake ◽  
High Fructose

High-fructose diet induced changes in gut microbiota structure and function, which have been linked to inflammatory response. However, the effect of small or appropriate doses of fructose on gut microbiota and inflammatory cytokines is not fully understood. Hence, the abundance changes of gut microbiota in fructose-treated Sprague-Dawley rats were analyzed by 16S rRNA sequencing. The effects of fructose diet on metabolic disorders were evaluated by blood biochemical parameter test, histological analysis, short-chain fatty acid (SCFA) analysis, ELISA analysis, and Western blot. Rats were intragastrically administered with pure fructose at the dose of 0 (Con), 2.6 (Fru-L), 5.3 (Fru-M), and 10.5 g/kg/day (Fru-H) for 20 weeks. The results showed that there were 36.5% increase of uric acid level in the Fru-H group when compared with the Con group. The serum proinflammatory cytokines (IL-6, TNF-α, and MIP-2) were significantly increased ( P < 0.05 ), and the anti-inflammatory cytokine IL-10 was significantly decreased ( P < 0.05 ) with fructose treatment. A higher fructose intake induced lipid accumulation in the liver and inflammatory cell infiltration in the pancreas and colon and increased the abundances of Lachnospira, Parasutterella, Marvinbryantia, and Blantia in colonic contents. Fructose intake increased the expressions of lipid accumulation proteins including perilipin-1, ADRP, and Tip-47 in the colon. Moreover, the higher level intake of fructose impaired intestinal barrier function due to the decrease of the expression of tight junction proteins (ZO-1 and occludin). In summary, there were no negative effects on body weight, fasting blood glucose, gut microbiota, and SCFAs in colonic contents of rats when fructose intake is in small or appropriate doses. High intake of fructose can increase uric acid, proinflammatory cytokines, intestinal permeability, and lipid accumulation in the liver and induce inflammatory response in the pancreas and colon.

Kombuchas from green and black teas reduce oxidative stress, liver steatosis and inflammation, and improve glucose metabolism in Wistar rats fed a high-fat high-fructose diet

2021 ◽  
Author(s):  
Rodrigo Cardoso ◽  
Luiza Dias Moreira ◽  
Mirian Costa ◽  
Renata Celi Lopes Toledo ◽  
Mariana Grancieri ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Lipid Metabolism ◽  
Glucose Metabolism ◽  
Wistar Rats ◽  
Liver Steatosis ◽  
Black Tea ◽  
High Fat ◽  
High Fructose Diet ◽  
High Fructose ◽  
Green And Black Tea

The aim of this study was to evaluate the effect of green and black tea kombuchas consumption on adiposity, lipid metabolism, liver steatosis, oxidative stress, and inflammation in Wistar rats...

Acute emotional stress and high fat/high fructose diet modulate brain oxidative damage through NrF2 and uric acid in rats

2020 ◽  
Vol 79 ◽  
pp. 23-34
Author(s):  
C. Batandier ◽  
T. Poyot ◽  
N. Marissal-Arvy ◽  
K. Couturier ◽  
F. Canini ◽  
...  
Keyword(s):  
Uric Acid ◽  
Oxidative Damage ◽  
Emotional Stress ◽  
High Fat ◽  
High Fructose Diet ◽  
Acute Emotional Stress ◽  
High Fructose

scholarly journals The Interactive Effect of High Doses of Chromium(III) and Different Iron(III) Levels on the Carbohydrate Status, Lipid Profile, and Selected Biochemical Parameters in Female Wistar Rats

Nutrients ◽  
2020 ◽  
Vol 12 (10) ◽  
pp. 3070
Author(s):  
Halina Zofia Staniek ◽  
Ewelina Król ◽  
Rafał Wojciech Wójciak
Keyword(s):  
Insulin Resistance ◽  
Lipid Profile ◽  
Carbohydrate Metabolism ◽  
Biochemical Parameters ◽  
Wistar Rats ◽  
Interactive Effect ◽  
The Other ◽  
Iron Deficient ◽  
Female Wistar Rats ◽  
High Doses

The aim of the study was to evaluate the main and interactive effects of chromium(III) propionate complex (Cr3) supplementation and different iron supply on the carbohydrate metabolism, lipid profile and other selected biochemical parameters of rats. The experiment was carried out in a two-factor design, in which rats were fed a diet with different proportions of Fe(III) and Cr(III) for six weeks. Fifty-four healthy female Wistar rats were divided into nine experimental groups with different Fe(III) levels, i.e. adequate—control group (45 mg/kg)—100% recommended daily dietary dose of Fe for rodents, deficient (5 mg/kg) and oversupply (180 mg/kg—400%). At the same time they were supplemented with Cr(III) of doses 1 (adequate), 50 and 500 mg/kg of diet. The activity and concentrations of most biochemical parameters were measured with standard enzymatic, kinetic, and colorimetric methods. HOMA-IR and QUICKI indexes were calculated according to appropriate formulas. It was found that there was an interactive effect of high Cr(III) doses and different Fe(III) levels in the diet on the carbohydrate metabolism and insulin resistance indexes. The presented results suggested that iron deficient diet fed animals led to insulin resistance; however, an effect is attenuated by Cr(III) supplementation at high doses. There were no significant changes in the rats’ lipid profile (except for the high density lipoprotein cholesterol (HDL-C) level) and most of the other biochemical parameters, such as the leptin, aspartate aminotransferase (AST), alanine transaminase (ALT), total protein (TP), creatinine (Crea) and the urea (BUN) concentrations. The study proved that the Cr(III) supplementation, independently and in combination with diversified Fe(III) content in the diet, affected the carbohydrate metabolism and insulin resistance indexes but did not affect lipid profile and most of the other biochemical parameters in healthy rats. The findings proved the role of Fe and Cr(III) and their interactions on disturbances carbohydrates metabolism.

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