scholarly journals Antimalarial Activity of Crude Extract and Solvent Fractions of the Leaves of Bersama abyssinica Fresen. (Melianthaceae) against Plasmodium berghei Infection in Swiss Albino Mice

2020 ◽  
Vol 2020 ◽  
pp. 1-14 ◽  
Author(s):  
Agumas Alemu Alehegn ◽  
Jibril Seid Yesuf ◽  
Eshetie Melese Birru
Keyword(s):  
Body Weight ◽  
Survival Time ◽  
Rectal Temperature ◽  
Crude Extract ◽  
Plasmodium Berghei ◽  
Antimalarial Activity ◽  
Aqueous Fraction ◽  
Albino Mice ◽  
Bersama Abyssinica

Background. Treatment of malaria has been compromised by the emergence of drug-resistant parasites. Consequently, novel agents are urgently needed from different sources including from medicinal plants. Thus, the current study aimed at evaluating the antimalarial activity of crude extract and solvent fractions of the leaves of Bersama abyssinica (B. abyssinica) against Plasmodium berghei infection in Swiss Albino mice. Method. A 4-day suppressive test was employed to evaluate the antimalarial effect of crude extract and solvent fractions against early infection. The curative and prophylactic effects of crude extract and fraction with the highest chemosuppression were further tested by Rane’s test and residual infection procedure. Parasitemia, survival time, packed cell volume (PCV), body weight, and rectal temperature of mice were used as evaluation parameters. Windows SPSS version 20 was used to analyze the data and analysis of variance (ANOVA) followed by Tukey’s post hoc test was used to compare data between groups. Results. The crude extract and aqueous fraction significantly (P<0.05 to 0.001) suppressed parasitemia followed by protection of PCV reduction resulting in prolonging the survival time but failed to protect body weight and rectal temperature reduction in all tested models. The ethyl acetate and chloroform fractions also showed significant chemosuppression and PCV protection in the 4-day suppressive test. The crude extract exhibited a chemosuppression of 49.51%, 57.94%, and 44.11% while the aqueous fraction showed suppression of 47.69%, 51.62%, and 37.07% in 4-day suppressive, curative, and prophylactic tests, respectively, at 400 mg/kg. Conclusion. The crude extract and fractions showed fairly moderate antimalarial activity, and the finding supports the traditional claims and previous in vitro studies. Thus, this may call for further studies to isolate chemical entities for additional safety and efficacy tests.

scholarly journals Antimalarial activity of hydromethanolic extract and its solvent fractions of Vernonia amygdalina leaves in mice infected with Plasmodium berghei

2019 ◽  
Vol 7 ◽  
pp. 205031211984976 ◽  
Author(s):  
Temesgen Bihonegn ◽  
Mirutse Giday ◽  
Getnet Yimer ◽  
Abebe Animut ◽  
Mekonnen Sisay
Keyword(s):  
Weight Loss ◽  
Survival Time ◽  
Rectal Temperature ◽  
Crude Extract ◽  
Plasmodium Berghei ◽  
Methanol Extract ◽  
Antimalarial Activity ◽  
Vernonia Amygdalina ◽  
Oral Toxicity

Background: Vernonia amygdalina Del. (Asteraceae) is reported to be traditionally used for the treatment of malaria. Based on folkloric repute of this plant in Ethiopian traditional medicine and crude extract-based ethnopharmacological studies conducted in few countries, this study was undertaken to evaluate the in vivo antimalarial activity of 80% methanol extract and its solvent fractions of the leaves of V. amygdalina in mice infected with Plasmodium berghei. Methods: A 4-day suppressive test was conducted on mice infected with P. berghei to find out antimalarial effect of chloroform, butanol and aqueous fractions obtained from the 80% methanol crude extract. In all the activity tests, mice were randomly assigned in five groups (three tests and two controls) of six animals in each and received respective treatments. Data were analyzed using one way analysis of variance followed by Tukey’s post hoc test for multiple comparisons. Results: Acute oral toxicity test showed that all solvent fractions of the leaves of V. amygdalina revealed neither mortality nor overt signs of toxicity up to 2000 mg/kg. This study indicated that the percentage parasitemia suppression of 80% methanol extract was 32.47% (±2.65), 35.40% (±3.14) and 37.67% (±2.50) at 200, 400 and 600 mg/kg, respectively. All doses of the 80% methanol extract of V. amygdalina prolonged survival time and prevented weight loss and packed cell volume reduction in infected mice. All doses of chloroform and butanol fractions significantly suppressed parasitemia (p < 0.05), increased survival time (p < 0.05) compared to negative control and exhibited a significant reduction in rectal temperature (p < 0.05). All solvent fractions significantly prevented weight loss (p < 0.05) at all tested doses. The 80% methanol extract and chloroform and butanol fractions significantly (p < 0.05) prevented further reduction in rectal temperature of P. berghei-infected mice at all doses. Conclusion: The results of this study indicated that 80% methanol extract and solvent fractions of the leaves of V. amygdalina demonstrated promising antimalarial activity. The study corroborated the folklore use of this plant for the treatment of malaria in ethnomedicine in Ethiopia.

scholarly journals Antimalarial Activity of Tinospora baenzigeri against Plasmodium berghei-Infected Mice

2019 ◽  
Vol 2019 ◽  
pp. 1-6 ◽  
Author(s):  
Sakaewan Ounjaijean ◽  
Manas Kotepui ◽  
Voravuth Somsak
Keyword(s):  
Weight Loss ◽  
Body Weight ◽  
Survival Time ◽  
Cell Volume ◽  
Crude Extract ◽  
Plasmodium Berghei ◽  
Packed Cell Volume ◽  
Antimalarial Activity ◽  
Body Weight Loss ◽  
Mean Survival Time

Plant species of the genus Tinospora (Menispermaceae) possess several pharmacological properties, and T. crispa has been reported to have antimalarial activity. T. baenzigeri (Chingcha Chalee) is a rich source of terpenes and quinoline alkaloids; however, it still has not yet been investigated the antimalarial activity of this plant extract. Hence, this study was aimed to evaluate the antimalarial activity of T. baenzigeri stem extract against Plasmodium berghei-infected mice. The aqueous crude extract of T. baenzigeri stem was prepared using a microwave-assisted method and tested for acute toxicity in mice. For evaluating the antimalarial activity in vivo, the standard 4-day test was carried out using groups of ICR mice infected with P. berghei ANKA administered orally by gavage with the extract (100, 250, and 500 mg/kg) for 4 consecutive days. Parasitemia, body weight, packed cell volume, and mean survival time were then measured. It was found that the aqueous crude extract of T. baenzigeri stem did not exhibit any sign of toxicity up to the dose of 2,000 mg/kg. The extract significantly (P<0.01) inhibited parasitemia in a dose-dependent manner, with 22.02%, 50.81%, and 74.95% inhibition. Moreover, the marked prevention of body weight loss and packed cell volume reduction was observed at doses of 100, 250, and 500 mg/kg of extract-treated mice. Additionally, the extract prolonged the mean survival time of P. berghei-infected mice, compared to the untreated group. In conclusion, the aqueous crude extract of T. baenzigeri stem has demonstrated potent antimalarial activity against P. berghei-infected mice with prolonged mean survival time and prevention of body weight loss and packed cell volume reduction.

Antimalarial activity of seed extracts of Schinus molle against plasmodium berghei in mice

2020 ◽  
Author(s):  
Abebe Basazn Mekuria ◽  
Mestayet Geta ◽  
Eshetie Melese Birru ◽  
Desalegn Asmelashe Gelayee
Keyword(s):  
Body Weight ◽  
Cell Volume ◽  
Crude Extract ◽  
Plasmodium Berghei ◽  
Packed Cell Volume ◽  
Antimalarial Activity ◽  
Aqueous Fraction ◽  
Schinus Molle ◽  
Isolation And Characterization

Abstract Background: Due to drug resistance and inefficient eradication techniques, malaria continues to be a major public health issue in countries with low- and middle-income. The seeds of Schinus molle are used in the Ethiopian folklore medicine for the treatment of malaria. However, this claim is not yet supported with scientific researches. Hence, the current study aims to investigate in vivo, antimalarial activity of hydro-alcoholic crude extract and subsequent solvent fraction of Schinus molle seeds on Plasmodium berghe infected mice.Methods: A hydro-alcoholic crude extract and solvent fractions (ethyl acetate, chloroform and aqueous) of Schinus molle seeds were tested at different doses (100, 200 and 400 mg/kg respectively ) to evaluate in vivo antimalarial activity of extracts in a 4-day suppressive, curative, and prophylaxis antimalarial test models. The parasitemia level, packed cell volume, survival of date, body weight, and body temperature were used to evaluate the anti-plasmodia activity of the extracts. One way ANOVA was employed to analyze these data, followed by post hoc Tukey’s HSD multiple comparison test.Results: The chemo-suppressive activities produced by the highest dose (400mg/kg) of crude extract and the aqueous fraction of Schinus molle seeds in the four-day suppressive test were 76.03% and 73.82%(p<0.001), respectively. In the curative test, the highest dose of crude and the aqueous fraction of Schinus molle seeds had 82.12% and 84.30% (p<0.001) suppression activity, respectively. The percentage of suppression in the prophylactic activities test of the aqueous fraction was 79.78% (p<0.001) at 400mg/kg compared to the negative control group. The studied plant extracts were likely anticipated to show rapid rectal temperature reduction and weight loss significantly. Among the extracts, only chloroform fraction has prevented the reduction of packed cell volume, due to the absence of saponin in the fraction. The mice which were treated with crude extract and aqueous fraction survived longer and gained net body weight as compared to vehicle-treated mice (p<0.001).Conclusion: The crude extract and aqueous fraction of Schinus molle seeds possessed significant antimalarial activity. These results collectively indicate that the plant has promising anti-plasmodial activity against Plasmodium berghei. However, further confirmatory studies followed by isolation and characterization of the active antimalarial compound are recommended.

scholarly journals Antimalarial Activity of Ethyl Acetate Extract and Fraction of Bidens pilosa against Plasmodium berghei (ANKA)

2020 ◽  
Vol 2020 ◽  
pp. 1-8 ◽  
Author(s):  
Noumedem Anangmo Christelle Nadia ◽  
Yamssi Cédric ◽  
Simeni Njonnou Sylvain Raoul ◽  
Ngongang Ouankou Christian ◽  
Mounvera Abdel Azizi ◽  
...  
Keyword(s):  
Ethyl Acetate ◽  
Crude Extract ◽  
Plasmodium Berghei ◽  
Ethyl Acetate Extract ◽  
Antimalarial Activity ◽  
Hematological Parameters ◽  
Control Group ◽  
Bidens Pilosa

Background. Malaria is one of the most critical diseases causing about 219 million cases worldwide in developing countries. The spread and development of resistance against chemical antimalarial drugs is one of the major problems associated with malaria control. The present study was to investigate the antimalarial efficacy of ethyl acetate extract and one fraction of Bidens pilosa in vivo in order to support the usage of this plant by traditional healers to treat malaria. Methods. The extracts were prepared by maceration of B. pilosa leaf powder in ethyl acetate. The liquid filtrate of the extract and the best in vitro antiplasmodial fraction using HPLC were concentrated and evaporated using a rotavapor under vacuum to dryness. The antimalarial activity of B. pilosa plant products were evaluated in vivo against Plasmodium berghei infected mice according to the Peter and Rane test. The antimalarial efficacy of the a selected crude extract (ethyl acetate extract) was evaluated at 125, 250, and 500 mg/kg, while a selected fraction from ethyl acetate extract (fraction 12) was evaluated at 62.5 and 125 mg/kg. Blood from experimental animals was collected to assess hematological parameters. Results. The crude extract of ethyl acetate and fraction 12 demonstrated 100% in vivo parasite suppressive activity at doses of 500 mg/kg and 125 mg/kg, respectively, for the crude extract and fraction 12. The mice treated with 250 and 500 mg/kg had their parasitemia (intraerythrocytic phase of P. Berghei) drop considerably, disappearing by the 8th day in mice receiving 500 mg/kg. The ethyl acetate extract of B. pilosa, fraction 12 showed an even higher antiplasmodial activity. By the 5th day of the experiment, the treatment led to a modification of hematological parameters in mice. The chloroquine (5 mg/kg), fraction 12 (125 mg/kg), and the crude extract (500 mg/kg) groups all survived the 30 days of the experiment, while the negative control group registered 100% of the deaths. Conclusion. This study scientifically supports the use of Bidens pilosa leaves in the traditional treatment of malaria. However, the mode of action and in vivo toxicity of the plant still need to be assessed.

scholarly journals Evaluation of antimalarial and antioxidant activities of the methanol seed extract of Adenanthera pavonina (Linn) in Plasmodium berghei infected mice

2014 ◽  
Vol 5 (4) ◽  
pp. 44-51 ◽  
Author(s):  
Aduragbenro Deborah Adedapo ◽  
Juliet N Olayinka ◽  
Oyindamola O. Abiodun ◽  
Ademola A Oyagbemi ◽  
Odunayo Azeez ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Crude Extract ◽  
Plasmodium Berghei ◽  
Antioxidant Activities ◽  
Antimalarial Activity ◽  
Seed Extract ◽  
Control Group ◽  
Untreated Control Group ◽  
Adenanthera Pavonina

Objectives: Malaria and oxidative stress are major health problems in the world in general. The goal of the study is to investigate the antimalarial and antioxidant activities of the methanol seed extract of Adenanthera pavonina linn (ADP) in Plasmodium berghei infected mice. Methods: Thirty five mice distributed into seven groups of five animals each were used in this study. Plasmodium berghei, was inoculated into Swiss albino mice intraperitoneally with an innoculum size of 1x107 on day zero (D0). The vehicle (1% DMSO), ADP (100, 200, 400, 600 and 800 mg/kg dose) or chloroquine (10 mg/kg) were thereafter administered from D0 – D3. At the end of the antimalarial studies, the blood samples from these animals were collected through cardiac puncture for biochemical assay. The effect of the ADP on the biomakers of oxidative stress was determined in infected mice. In addition in vitro antioxidant activities of ADP were assessed using the 1, 1-diphenyl-2-picrylhydrazyl (DPPH) based assay. Results: The percentage parasitemia decreased significantly in the parasitized treated group with the crude extract (p < 0.001) compared to the parasitized untreated control group. Also the crude extract, at a dose of 800 mg/kg exerted an antimalarial activity (92.11%) higher than that of chloroquine (88.73%). In the in vitro antioxidant studies, the extract had an IC50> 400 μg/ml which was significantly higher than the standard antioxidant drug, ascorbic acid (IC50 = 1.20 μg/ml). In the case of biochemical and in vivo assay, there was no statistical significant difference (p >0.05) in plasma total protein, malondialdehyde (MDA) and hydrogen peroxide (H2O2) levels in all the treated groups compared to the parasite control group but, there was a statistical significant decrease (p < 0.05) in glutathione (GSH) levels at doses of 400 and 800 mg/kg compared to the parasitized untreated control group. Conclusions: Methanol seed extract of Adenanthera pavonina demonstrated a significant antimalarial activity but did not exert any antioxidant effect over the parasitized treated mice. DOI: http://dx.doi.org/10.3126/ajms.v5i4.9107 Asian Journal of Medical Sciences 2014 Vol.5(4); 44-51

scholarly journals Evaluation of Antimalarial Activity of Methanolic Root Extract of Myrica salicifolia A Rich (Myricaceae) Against Plasmodium berghei–Infected Mice

2020 ◽  
Vol 25 ◽  
pp. 2515690X2092053 ◽  
Author(s):  
Zemene Demelash Kifle ◽  
Getnet Mequanint Adinew ◽  
Mestayet Geta Mengistie ◽  
Abyot Endale Gurmu ◽  
Engidaw Fentahun Enyew ◽  
...  
Keyword(s):  
Crude Extract ◽  
Plasmodium Berghei ◽  
Antimalarial Activity ◽  
Effective Vaccine ◽  
Root Extract ◽  
Honestly Significant Difference ◽  
Significant Difference ◽  
And Control

Background. The management and control of malaria has become gradually challenging due to the spread of drug-resistant parasites, lack of effective vaccine, and the resistance of vector to insecticides. Consequently, novel agents are urgently needed from different sources including from medicinal plants. In Ethiopia and Uganda, Myrica salicifolia root is traditionally claimed for the treatment of malaria. The aim of this study was to evaluate the in vivo antimalarial activity of root crude extract of M salicifolia. Methods. The parasite, Plasmodium berghei was used in this study since it is an appropriate parasite that is most commonly used because of its higher accessibility. A 4-day suppressive test was employed to evaluate the antimalarial effect of crude extract against early infection. The curative and prophylactic effect of the crude extract was further tested by Rane’s test and residual infection procedure. Parasitemia, survival time, packed cell volume, body weight, and rectal temperature of mice were used as evaluation parameters. Windows SPSS version 24 was used to analyze the data and analysis of variance followed by Tukey’s honestly significant difference to compare results between groups. Results. The root crude extract of M salicifolia significantly ( P < .05-.0001) suppressed parasitemia. The crude extract exhibited a chemosuppression of 40.90. Conclusion. The development of new antimalarial agents and the finding supports the traditional claims and previous in vitro studies.

scholarly journals Antimalarial Activity of Aqueous and 80% Methanol Crude Seed Extracts and Solvent Fractions of Schinus molle Linnaeus (Anacardiaceae) in Plasmodium berghei-Infected Mice

2020 ◽  
Vol 2020 ◽  
pp. 1-9 ◽  
Author(s):  
Getu Habte ◽  
Teshome Nedi ◽  
Solomon Assefa
Keyword(s):  
Acute Toxicity ◽  
Plasmodium Berghei ◽  
Antimalarial Activity ◽  
Antimalarial Drugs ◽  
Negative Control ◽  
Aqueous Fraction ◽  
Schinus Molle ◽  
Crude Extracts

Background. Malaria is among the leading causes of mortality and morbidity. Moreover, the emergence of resistance to antimalarial drugs is a major problem in controlling the disease. This makes the development of novel antimalarial drugs a necessity. Medicinal plants are important sources in discovering antimalarial drugs. Schinus molle is claimed for its antimalarial effect in Ethiopian folkloric medicine and endowed with in vitro antiplasmodial activity. In the present study, the in vivo antimalarial activity of the plant was investigated. Methods. Acute toxicity was carried out using a standard procedure. To screen the in vivo antimalarial potential of the S. molle against Plasmodium berghei (ANKA), a 4-day suppressive test was employed. The extracts and fractions were given to infected mice by oral gavage at 100, 200, and 400 mg/kg/day for four consecutive days. Parameters such as parasitemia were then evaluated. Results. Any sign of toxicity was not observed in the oral acute toxicity test. The crude extracts and solvent fractions exerted a significant (p<0.05) inhibition of parasite load compared to the negative control. The highest inhibition (66.91%) was exhibited by the 400 mg/kg/day dose of 80% methanolic crude extract. Among the fractions, chloroform fraction demonstrated maximal chemosuppressive effect (55.60%). Moreover, crude extracts and solvent fractions prevented body weight loss, reduction in temperature, and anemia compared to the negative control. Except the aqueous fraction, the tested plant extracts were able to significantly prolong the survival time of infected mice. Conclusion. The findings of the present study confirmed the safety and a promising in vivo antimalarial activity of S. molle, thus supporting the traditional claim and in vitro efficacy. In-depth investigations on the plant, however, are highly recommended.

scholarly journals In vivo antimalarial activity of the crude leaf extract of Combretum molle against Plasmodium berghei in mice

2020 ◽  
Author(s):  
Melkamu Adigo Shibeshi ◽  
Tezera Jemere Aragaw ◽  
Getnet Mequanint Adinew ◽  
Engdaw Fentahun Enyew
Keyword(s):  
Body Temperature ◽  
Survival Time ◽  
Cell Volume ◽  
Crude Extract ◽  
Plasmodium Berghei ◽  
Packed Cell Volume ◽  
Antimalarial Activity ◽  
Swiss Albino Mice ◽  
Combretum Molle

Abstract Background Malaria is an infectious, hematologic disease causing death and illness in children and adults, especially in tropical countries. The aim of this study was to evaluate the antimalarial activity of Combretum molle extract in vivo assays against Plasmodium berghei in Swiss albino mice. Methods Plasmodium berghei a rodent malaria parasite was inoculated to healthy Swiss Albino mice age 6–8 weeks either sex, weight 20–33g. 100, 200 and 400mg/kg/day of Crude methanolic extract of Combretum molle were administered. Parameters such as Percent parasitemia, body weight, Body temperature, packed cell volume and survival time were then determined using standard tests. Data were analyzed using one-way ANOVA followed by the Post hoc Tukey HSD test with SPSS software version 24.0 and P ≤0.05 considered as statistically significant. Results Chemosuppresive effect exerted by the crude extract ranged between 27-68%. The curative effect of the crude extract was in the range of 25-49% and ptophylactic effect of the crude extract was in the range of 51–76.2%%. The maximum effect in all tests on Chemosuppresive, curative, Prophylactic, prevention of weight loss, body temperature and packed cell volume and an increase in mean survival time was observed at higher doses of the crude extract. Conclusion From the present study it can be concluded that the crude extract of Combretum molle leaves has been shown promising antimalarial activity. This finding supports the traditional use of the plant for the treatment of malaria in Ethiopia. Thus, it could be considered as a potential source to develop safe, effective and affordable antimalarial agent.

scholarly journals Antimalarial Activity of Hydromethanolic Crude Extract and Chloroform Fraction of Brucea antidysenterica Leaves in Plasmodium berghei-Infected Mice

2021 ◽  
Vol 2021 ◽  
pp. 1-14
Author(s):  
Tezera Jemere Aragaw ◽  
Kefyalew Ayalew Getahun
Keyword(s):  
Crude Extract ◽  
Plasmodium Berghei ◽  
Antimalarial Activity ◽  
Negative Control ◽  
Chloroform Fraction ◽  
Curative Effect ◽  
Antimalarial Agents ◽  
Different Doses

Background. Different parts of Brucea antidysenterica are used in traditional and alternative medicine in Ethiopia for the treatment of different health problems including malaria and have good in vitro antimalarial activity. However, no in vivo study was conducted to substantiate the claim. Our study planned to determine the antimalarial effect of B. antidysenterica extract. Methods. Swiss albino mice (6–8 weeks old, 20–28 g) were inoculated with Plasmodium berghei. Different doses of both hydromethanolic extract and chloroform fraction were orally given at 100, 200, and 400 mg/kg/day. Results. The parasitemia suppression percent of hydromethanolic crude extract and chloroform fraction in chemosuppressive tests ranged between 33.48 and 75.93% and 38.32 and 76.64%, respectively. The hydromethanolic crude extract and chloroform fraction exhibited the curative effect of 46.75–70.91% and 50.30–80.06% parasitemia suppression, respectively ( p  < 0.001), compared with negative control. Conclusion. From our study, it is concluded that the hydromethanolic crude extract and chloroform fraction of B. antidysenterica leaves showed promising antiplasmodial effects against Plasmodium berghei. This upholds the folkloric use of B. antidysenterica leaves and the thought of as a possible source to develop new antimalarial agents.

scholarly journals Evaluation of the Antimalarial Activity of Crude Extract of Eucalyptus globulus Labill. Leaf against Plasmodium berghei in Mice

2021 ◽  
Vol 2021 ◽  
pp. 1-9
Author(s):  
Mihret Ayalew ◽  
Azmeraw Bekele
Keyword(s):  
Body Weight ◽  
Survival Time ◽  
Cell Volume ◽  
Crude Extract ◽  
Packed Cell Volume ◽  
Antimalarial Activity ◽  
Negative Control ◽  
Common Source ◽  
In Vitro Test ◽  
Traditional Use

Introduction. Traditional medicinal plants are used as a common source of developing new and effective antimalarial drugs. E. globulus leaf has been used in the traditional management of malaria in different countries, including Ethiopia. However, there is no in vivo study done on the antimalarial activity of the plant. Thus, this study aimed to evaluate the antimalarial activity of crude extract of E. globulus Labill. leaf in P. berghei-infected mice. Method. The fresh leaves of E. globulus were collected, washed, air-dried, and made as coarse powder. Either sex of mice aged 6 to 8 weeks was used in the experiment. The antimalarial activity of the crude extract was tested in four-day suppressive, curative (Rane’s), and prophylactic (repository) tests. The parameters like level of parasitemia, packed cell volume, body weight, rectal temperature, and mean survival time were recorded. The analysis of the data was done with SPSS version 20 with a 95% confidence interval in one-way ANOVA followed by Tukey’s post hoc test. Results. In all three antimalarial test models, the extract of leaf of E. globulus at all three doses suppressed the level of parasitemia significantly ( p < 0.001 ), increased survival time ( p < 0.05 to p < 0.001 ), and prevented a decrease in body weight as compared to the negative control. The middle and large doses of the extract also decreased loss of body temperature ( p < 0.05 to p < 0.001 ) compared to the negative control. Reduction of packed cell volume was prevented within the three test doses of the extract in both curative and prophylactic tests and middle and large doses in the 4-day suppressive test compared to the negative control. Conclusion. The crude extract of the plant showed promising antimalarial activity. This supports the traditional use and the in vitro test result of the plant.

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