scholarly journals Compatibility of Composite Biomaterials in Sports Injury Repair

2021 ◽  
Vol 2021 ◽  
pp. 1-9
Author(s):  
Mo Xing
Keyword(s):  
Cell Proliferation ◽  
Survival Rate ◽  
Tricalcium Phosphate ◽  
Sports Injuries ◽  
Composite Scaffold ◽  
Apoptosis Rate ◽  
Cell Proliferation Activity ◽  
Proliferation Activity ◽  
Experimental Group ◽  
Hemolysis Rate

With the rapid development of sports, sports injuries have become an inevitable problem in sports. Repairing sports injuries has always been the focus of attention in the field of sports and medicine. The wide application of biomedical materials leads to the innovation and development of medical technology. It is of great significance to use them to repair sports injuries, but the current research results are not ideal. In this paper, the biocompatibility of composite biomaterials used to repair sports injuries was studied. First, the rat bone marrow mesenchymal stem cells were cultured to prepare a cell suspension. Then, the tricalcium phosphate-gelatin composite scaffold material and the collagen material were scraped into powder to make the material extract, which are, respectively, the experimental group and the control group. An apoptosis detection kit and a flow cytometer were used to detect apoptosis rate and survival rate of all samples, and a CKK-8 kit was used to detect cytotoxicity and calculate cell proliferation activity. The absorbance value was read with a microplate reader, and the coagulation performance and hemolysis rate of different groups were calculated. The experimental results showed that the cell survival rate of the experimental group was 91.41%, the early apoptosis rate was 2.81%, the stem cell proliferation activity was 0.83, and the anticoagulant performance and hemolysis rate were 64.8% and 0.28%, respectively. This indicates that although the anticoagulant performance of the tricalcium phosphate-gelatin composite scaffold is weak, its cell proliferation activity is strong, which is more suitable for cell adhesion and proliferation, and the cell apoptosis and hemolysis rate are lower. Therefore, it has better biocompatibility than a single collagen material.

Cyclin D1 expression in astrocytomas is associated with cell proliferation activity and patient prognosis

1999 ◽  
Vol 188 (3) ◽  
pp. 289-293 ◽  
Author(s):  
Satu-Leena Sallinen ◽  
Pauli K. Sallinen ◽  
Juha T. Kononen ◽  
Kirsi M. Syrj�koski ◽  
Nina N. Nupponen ◽  
...  
Keyword(s):  
Cell Proliferation ◽  
Cyclin D1 ◽  
Cell Proliferation Activity ◽  
Proliferation Activity ◽  
Patient Prognosis

scholarly journals The Activity of Combination of Hydrolyzed Virgin Coconut Oil and Chitosan Toward Wound Healing Parameters on NIH 3T3 Cells Using in Vitro Methods

1970 ◽  
Vol 7 (3) ◽  
pp. 14-19 ◽  
Author(s):  
Hekdin Marsius Sipayung ◽  
Jansen Silalahi ◽  
Yuandani Y
Keyword(s):  
Cell Proliferation ◽  
Wound Healing ◽  
Protein Expression ◽  
Scratch Wound ◽  
Cell Proliferation Activity ◽  
Proliferation Activity ◽  
Cox 2 ◽  
Nih 3T3 ◽  
Vegf Protein

Objectives: The objective of this study was to investigate the activity of combination of hydrolyzed VCO (HVCO) and chitosan on NIH 3T3 cell proliferation activity, NIH 3T3 cell migration, COX-2 and VEGF protein expression. Design: In vitro cytotoxic assay was determined by MTT (MicrocultureTetrazoliumTehnique) assay, cell proliferation activity was measured by calculating cell viability incubated 24 hours, 48 hours and 72 hours, wound closure percentage was tested by scratch wound healing method, expression of COX-2 protein and VEGF protein were measured by immunocytochemical method. Interventions: The variable that was intervened in this study was the concentration of HVCO and chitosan. Main Outcome Measures: The main measurements carried out in this study were the absorbance value of HVCO and chitosan which was converted into viability cell, proliferation activity, percentage of wound closure, and percentage of COX-2 and VEGF protein expression. Results: Cytotoxic activity of HVCO and chitosan resulted the best concentration at 31.25 μg/ml, scratch wound healing assay from a combination HVCO and chitosan resulted the best migration of fibroblast cells at a ratio of 1:1 with HVCO 62.5 μg/ml and chitosan 62.5 μg/ml, combination of HVCO 62.5 μg/ml and chitosan 62.5 μg/ml (1:1) increased expression of COX-2 and VEGF. Conclusion: Combination of HVCO and chitosan could increase NIH 3T3 cell migration, COX-2 and VEGF protein expression. Combination of HVCO and chitosan had better wound healing activity in vitro than single use. Keywords: Rhizomucor miehei, viability, proliferation, migration, expression

scholarly journals ANTIOXIDANT AND CYTOTOXIC ACTIVITY OF COMBINED EXTRACTS PREPARED USING FICUS RELIGIOSA AND FICUS BENGHALENSIS LEAVES AGAINST CERVICAL CANCER CELL LINE (HELA)

2018 ◽  
Vol 11 (12) ◽  
pp. 407
Author(s):  
Suriya Kumaresan ◽  
Rema Ramasamy ◽  
Philip Robinson Jayachandran
Keyword(s):  
Cervical Cancer ◽  
Hydrogen Peroxide ◽  
Cell Proliferation ◽  
Cytotoxic Activity ◽  
Methanol Extract ◽  
Ficus Religiosa ◽  
Ficus Benghalensis ◽  
Cell Proliferation Activity ◽  
Reduction Assay ◽  
Proliferation Activity

Objectives: Medicinal plants and herbs are used in combination in Ayurveda and folklore medicine as they exhibit good cytotoxic activity. In the present study, the antioxidant, phytochemical, and cell proliferation activity of the combined crude methanolic extract of Ficus religiosa and Ficus benghalensis leaves were investigated.Methods: Antioxidant activity was performed by 2, 2-diphenyl-1-picryl-hydrazyl-hydrate (DPPH) and hydrogen peroxide methods, and the presence of the phytochemicals was screened using the gas chromatography–mass spectrometry. The extract was further evaluated for its cell proliferation activity against cancer cells using the mitochondrial reduction assay. Antioxidant property of the extracts was measured using the DPPH, hydrogen peroxide, and ferric-reducing antioxidant power assay, respectively, using the UV spectrophotometer.Results: The combined extract exhibited strong antioxidant potential in DPPH assay by increase in the percentage of inhibition with the increase in concentration. Similarly, the IC50 value of the methanol extract in peroxidase scavenging activity was 49.85 μg/mL comparatively lower than the ascorbic acid used as standard. The phytochemical analysis of the methanol extract showed the presence of nine phytoconstituents, which exhibit antioxidant and anticancer property. Mitochondrial reduction assay performed to evaluate the cell proliferation activity of the combined leave extract showed that increase in the concentration of the extract decreased the cell proliferation in the HeLa cell line.Conclusion: The results of present study show a possible synergistic activity of leaves against human cervical cancer.

Cell proliferation activity unrelated to COX-2 expression in ovarian tumors

2007 ◽  
Vol 17 (3) ◽  
pp. 607-614 ◽  
Author(s):  
A. YOSHIDA ◽  
L.O. SARIAN ◽  
L.A.L.A. ANDRADE ◽  
F. PIGNATARO ◽  
G.A. PINTO ◽  
...  
Keyword(s):  
Cell Proliferation ◽  
Ovarian Tumors ◽  
Cell Proliferation Activity ◽  
Proliferation Activity ◽  
Cox 2
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