scholarly journals Comparative Study of the Silver Nanoparticle Synthesis Ability and Antibacterial Activity of the Piper Betle L. and Piper Sarmentosum Roxb. Extracts

2021 ◽  
Vol 2021 ◽  
pp. 1-9
Author(s):  
Ngoc Hoi Nguyen ◽  
Tran Thi Yen Nhi ◽  
Ngo Thi Van Nhi ◽  
Tran Thi Thu Cuc ◽  
Pham Minh Tuan ◽  
...  

Piper betle (P. betle) and Piper sarmentosum (P. sarmentosum) are the two members of the Piper genus, have been reported to be rich in phytochemicals and essential oils, which showed strong reducing power, antibacterial, and antifungal activities. P. betle recently has been studied and applied in several commercial products in the antimicrobial respect, meanwhile its relatives, P. sarmentosum has been lesser-known in this field. In this study, the two Piper species—P. betle and P. sarmentosum were studied to compare their ability in silver nanoparticle synthesis and efficacy in antibacterial activity. P. betle and P. sarmentosum were extracted by distilled water at different temperatures and times. Subsequently, their total reducing capacity was determined by DPPH scavenging and Folin-Ciocalteu assays to choose the appropriate extraction conditions. The silver nanoparticle solutions prepared by the extracts of P. betle (Pb.ext) and P. sarmentosum (Ps.ext) were characterized by Dynamic light scattering (DLS), Zeta potential, UV-vis, and Fourier-transform infrared (FTIR) measurements. Finally, the antibacterial activity of the synthesized silver nanoparticle solutions was tested against Escherichia coli using the agar diffusion well–variant method. The Pb.ext showed stronger reducing power with higher total polyphenol content (~125 mg GAE/mL extract) and better DPPH activity (IC50~1.45%). Both the green synthesized silver nanoparticle solutions (Pb.AgNP and Ps.AgNP) performed significantly stronger antibacterial activity on Escherichia coli compared to their initial extracts. Antibacterial tests revealed that Ps.AgNP showed remarkably better growth inhibition activity as compared to Pb.AgNP. This study would contribute useful and important information to the development of antibacterial products based on green synthesized silver nanoparticles fabricated by the extracts of P. betle and P. sarmentosum.

2012 ◽  
Vol 9 (2) ◽  
pp. 197-209 ◽  
Author(s):  
S.C.G. Kiruba Daniel ◽  
B. Nazeema Banu ◽  
M. Harshiny ◽  
K. Nehru ◽  
P. Sankar Ganesh ◽  
...  

2018 ◽  
Vol 225 ◽  
pp. 101-104 ◽  
Author(s):  
R. Mythili ◽  
T. Selvankumar ◽  
S. Kamala-Kannan ◽  
C. Sudhakar ◽  
Fuad Ameen ◽  
...  

Author(s):  
Asima Khan ◽  
Qurban Ali ◽  
Muhammad Awais Javeid ◽  
Arif Malik

Aim of present research was to evaluate the anti-bacterial, anti-fungal, anti-oxidant and multi-stress activities of a medicinal plant Mint Lemonade plant extract. Fresh mint was purchased from the nearest market. The samples were cleaned with distilled led water and its leaves, stem and roots were separated them all. The material was dried in shade at room temperature (24ºC). The three types of extracts were prepared for each sample of mint plantviz, ethanol, n-hexane and water. The antibacterial and antifungal activities of ethanolic, n-haxene and aqueous mint extract was determined on Escherichia coli, Bacillus cereus, Pseudomonas, Aeromonas hydrophila, Aspergillus niger, Aspergillus flavous and Rhizopus stolonifer respectively. From the results of the antibacterial and antifungal activities it was clear that the aqueous extract showed no inhibitory effect on test these microorganisms. In ethanolic extract of antibacterial activity Escherichia coli showed the maximum zone of inhibition with diameters of 3.90 cm at 50 µl dose. Bacilluscereus showed the zone of inhibition diameter 4.9 cm. Pseudomonas showed the maximum zone of inhibition in diameter 3.86mm.Aeromonas hydrophila expressed the maximum zone of inhibition in diameter 3.86mm. The n-hexane extracts antibacterial activity of E. coli, Bacillus cereus, Pseudomonas and Aeromonas hydrophila showed the zone of inhibition 3.86 cm, 4.5 cm, 7.90 mm and 4.71 cm respectively, while the positive control showed inhibition zone about 0.0 cm. The antifungal activity in n-haxene extract showed the maximum result in Aspergillus flavous 18 cm of the area of inhibition. In ethanol extracts antifungal activity showed the maximum result in Aspergillus flavous 4.8 cm of the zone of inhibition. The inhibitory action of mint plant extracts indicated that the plant may be used as potential antibacterial agent.


Materials ◽  
2019 ◽  
Vol 12 (24) ◽  
pp. 4180 ◽  
Author(s):  
Qunying Yuan ◽  
Manjula Bomma ◽  
Zhigang Xiao

In this study, the metallothionein gene of Candida albicans (C. albicans) was assembled by polymerase chain reaction (PCR), inserted into pUC19 vector, and further transformed into Escherichia coli (E. coli) DH5α cells. The capacity of these recombinant E. coli DH5α cells to synthesize silver nanoparticles was examined. Our results demonstrated that the expression of C. albicans metallothionein in E. coli promoted the bacterial tolerance to metal ions and increased yield of silver nanoparticle synthesis. The compositional and morphological analysis of the silver nanoparticles revealed that silver nanoparticles synthesized by the engineered E. coli cells are around 20 nm in size, and spherical in shape. Importantly, the silver nanoparticles produced by the engineered cells were more homogeneous in shape and size than those produced by bacteria lack of the C. albicans metallothionein. Our study provided preliminary information for further development of the engineered E. coli as a platform for large-scale production of uniform nanoparticles for various applications in nanotechnology.


2019 ◽  
Vol 4 (48) ◽  
pp. 14276-14280
Author(s):  
M. Aminul Haque ◽  
M. Shamim Hossain ◽  
Md. Rajibul Akanda ◽  
Md. Aminul Haque ◽  
Shamsun Naher

2020 ◽  
Vol 27 (6) ◽  
pp. 1580-1586 ◽  
Author(s):  
Chita Ranjan Sahoo ◽  
Subhashree Maharana ◽  
Chinmayee Priyadarshini Mandhata ◽  
Ajit Kumar Bishoyi ◽  
Sudhir Kumar Paidesetty ◽  
...  

Author(s):  
Thanigaivel Sundaram ◽  
B. Indu ◽  
CH. Srinivasulu Reddy ◽  
V. S. Swathi Raj ◽  
S. Hari priya ◽  
...  

2020 ◽  
Vol 24 (19) ◽  
pp. 2272-2282
Author(s):  
Vu Ngoc Toan ◽  
Nguyen Minh Tri ◽  
Nguyen Dinh Thanh

Several 6- and 7-alkoxy-2-oxo-2H-chromene-4-carbaldehydes were prepared from corresponding alkyl ethers of 6- and 7-hydroxy-4-methyl-2-oxo-2H-chromen-2-ones by oxidation using selenium dioxide. 6- and 7-Alkoxy-4-methyl-2H-chromenes were obtained with yields of 57-85%. Corresponding 4-carbaldehyde derivatives were prepared with yields of 41-67%. Thiosemicarbazones of these aldehydes with D-galactose moiety were synthesized by reaction of these aldehydes with N-(2,3,4,6-tetra-O-acetyl-β-Dgalactopyranosyl) thiosemicarbazide with yields of 62-74%. These thiosemicarbazones were screened for their antibacterial and antifungal activities in vitro against bacteria, such as Staphylococcus aureus, Escherichia coli, and fungi, such as Aspergillus niger, Candida albicans. Several compounds exhibited strong inhibitory activity with MIC values of 0.78- 1.56 μM, including 8a (against S. aureus, E. coli, and C. albicans), 8d (against E. coli and A. niger), 9a (against S. aureus), and 9c (against S. aureus and C. albicans).


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