scholarly journals Effect of a Copaiba Oil-Based Dental Biomodifier on the Inhibition of Metalloproteinase in Adhesive Restoration

2021 ◽  
Vol 2021 ◽  
pp. 1-10
Author(s):  
Eliane Avany Malveira Araújo ◽  
Geisy Rebouças Lima ◽  
Luciana Aleixo dos Santos de Melo ◽  
Leilane Bentes de Sousa ◽  
Marne Carvalho de Vasconcellos ◽  
...  
Keyword(s):  
Qualitative Evaluation ◽  
Cytotoxicity Test ◽  
Significant Difference ◽  
Copaiba Oil ◽  
In Situ Zymography ◽  
Imagej Software ◽  
Antiproteolytic Activity ◽  
Zymographic Assay

Aim. This study sets out to evaluate the antiproteolytic activity of copaiba oil-based emulsion at the resin/dentin adhesive interface union formed with conventional and self-etching adhesives systems. Methods. At in situ zymography, 30 teeth were sectioned 2 mm below the enamel-dentin junction; a smear layer was standardized and subdivided into four groups. Gelatin conjugated with fluorescein was used and taken to the fluorescence microscope for evaluation. In cytotoxicity, the Trypan Blue method was used at four different time points. The tested groups were (G1) control with distilled water; (G2) 2% chlorhexidine (CLX); (G3) emulsion based on copaiba oil (EC) 10% + X; (G4) 10% EC + Y; and (G5) EC 10% alkaline. The zymographic assay used the same groups described, but in 30 seconds and 10 and 20 minutes. HT1080 cells were incubated and submitted to electrophoresis. The gel was analyzed using ImageJ software. Mann–Whitney and Kruskal–Wallis tests were used in the statistical analysis ( p < 0.05 ). Results. ECs showed higher cell viability in the cytotoxicity test and showed a significant difference in 10 and 20 minutes. In the zymographic assay, alkaline EC reduced 67% of MMP-2 activity and 44% of MMP-9 compared to 2% chlorhexidine. At in situ zymography in qualitative evaluation, all groups tested showed inhibition of activity in metalloproteinases. Conclusion. EC showed activity in the inhibition of metalloproteinases in vitro and in situ, especially the alkaline one. The survey shows the possibility of using ECs, a product from Amazonian biodiversity, as a biomodifier in dentistry.

scholarly journals Therapeutical aspect of trichomoniasis

2003 ◽  
Vol 131 (3-4) ◽  
pp. 156-162 ◽  
Author(s):  
Jelica Vukicevic ◽  
Jasmina Jankicevic
Keyword(s):  
Trichomonas Vaginalis ◽  
Communicable Disease ◽  
Repeated Administration ◽  
Transmitted Infection ◽  
Microbiological Test ◽  
Clinical Forms ◽  
Short Period ◽  
Significant Difference

Trichomoniasis is frequent, parasitic and sexually transmitted infection of genitourinary tract. It is treated by metronidazole (5-nitroimidazole) according to protocol recommended by Center for Disease Control (CDC formerly called: Communicable Disease Center) [19]. The resistance of Trichomonas vaginalis (TV) strains to metronidazole (MND) was described in USA in 1960, and later on in many European countries [8, 9, 10, 11, 12, 13]. In these cases, due to persistent trichomonas infection, it is necessary to repeat MND treatment with moderate modification of dose and/or length of its application. Nevertheless, oncogenic and toxic effects of MND have to be taken into consideration. OBJECT The aim of this study was to investigate and analyze the incidence of TV in STD and lower susceptibility of certain TV strains to MND were analyzed. MATERIAL AND METHODS In three-year period (1999-2001) 612 patients (244 females and 368 males) suspected of STD were examined clinically and microbiologically at the Institute of Dermatovenereology in Belgrade. The patients detected for TV were treated according to CDC protocol. The affected were considered cured if there was no manifest clinical infection, and no TV verified by microbiological test. Results TV was isolated in 216 patients (35.29 % of all subjects). Trichomonas infection was found in 90 (36.88 %) out of 244 tested females and in 126 (32.34 %) of 368 males. Clinically manifested infection, with extensive urethral and vaginal secretion, was recorded in 161 patients, while the asymptomatic form was found in 55 subjects. This result indicates the predominance of manifested trichomonas infections (75.54 % of cases). The difference of distribution of clinical forms of trichomoniasis, in relation to sex, was not statistically significant (c2=0.854; p>0.05). The patients with verified trichomonas infection were treated by metronidazole according to CDC protocol. The recommended therapeutical scheme consisted of three phases proceeding in succession, in so far TV had not been eliminated by previous one. The number of cured patients, according to therapeutical phases, was shown in Table 4. Three patients (1.39 %, 2 males and 1 female) were not cured in spite of all three completed phases of therapeutical protocol. In all three cases, TV was eliminated by MND application in dose of 3 g/daily, during two days. The failure of minute MND treatment was analyzed in relation to clinical forms of the infection (manifested or asymptomatic), as well as in relation to types of infection (single- or associated infection). The incidence of refractory trichomoniasis treated by a single metronidazole dose of 2 g was significantly higher in the group of patients with polyinfection (c2=18.270 p<0.01). There was no significant difference of resistance to a single MND dose between the groups with manifested and asymptomatic trichomoniasis (c2=0.321; p<0.01). The prevalence of TV in vaginal and urethral smears indicates the significant incidence of trichomoniasis in STD. TV was more frequently isolated in patients with clinically manifested infection. TV susceptibility to MND was tested in vitro in aerobic and anaerobic conditions. The resistance of strains under in vitro conditions did not correlate with refractory feature of trichomoniasis to MND application [7 17, 18]. The success of trichomoniasis treatment depends upon multiple factors, including: a) TV susceptibility to drug, b) intravaginal redox potential, c) drug concentration in situ, d) associated microorganisms that may modify the amount of the drug available in situ [7, 18, 21]. The results of our investigation argue for the latter item, verifying that TV resistance to MND is higher in patients with polyinfection in relation to those with monoinfection (significant difference, c2=18.270; p<0.01). Repeated administration of low metronidazole doses may prolong the therapy of trichomonas infections, while application of high doses (over 3 g/day) may result in undesired complications. Given the well-known fact that repeated sublethal doses induce the resistance, would it be more beneficial to begin with slightly higher metronidazole dose (3 g/day) during short period of time (3-5 days)? This will be the subject of our further investigation.

Effect of 10% and 15% Carbamide Peroxide on Fracture Toughness of Human Dentin In Situ

2013 ◽  
Vol 38 (2) ◽  
pp. 142-150 ◽  
Author(s):  
LE Tam ◽  
P Bahrami ◽  
O Oguienko ◽  
H Limeback
Keyword(s):  
Fracture Toughness ◽  
Placebo Group ◽  
Color Change ◽  
Carbamide Peroxide ◽  
Tooth Sensitivity ◽  
Custom Made ◽  
Dental Hard Tissues ◽  
Significant Difference

SUMMARY Purpose Although damage to the structural integrity of the tooth is not usually considered a significant problem associated with tooth bleaching, there have been some reported negative effects of bleaching on dental hard tissues in vitro. More studies are needed to determine whether the observed in vitro effects have practical clinical implications regarding tooth structural durability. Objectives This in situ study evaluated the effect of 10% and 15% carbamide peroxide (CP) dental bleach, applied using conventional whitening trays by participants at home, on the fracture toughness of dentin. Methods Ninety-one adult volunteers were recruited (n ≈ 30/group). Compact fracture toughness specimens (approximately 4.5 × 4.6 × 1.7 mm) were prepared from the coronal dentin of recently extracted human molars and gamma-radiated. One specimen was fitted into a prepared slot, adjacent to a maxillary premolar, within a custom-made bleaching tray that was made for each adult participant. The participants were instructed to wear the tray containing the dentin specimen with placebo, 10% CP, or 15% CP treatment gel overnight for 14 nights and to store it in artificial saliva when not in use. Pre-bleach and post-bleach tooth color and tooth sensitivity were also evaluated using ranked shade tab values and visual analogue scales (VASs), respectively. Within 24–48 hours after the last bleach session, the dentin specimens were tested for fracture toughness using tensile loading at 10 mm/min. Analysis of variance, Kruskal-Wallis, χ2, Tukey's, and Mann-Whitney U tests were used for statistical analysis. The level of significance was set at p&lt;0.05 for all tests, except for the Mann-Whitney U tests, which used a Bonferroni correction for post hoc analyses of the nonparametric data (p&lt;0.017). Results The placebo, 10% CP, and 15% CP groups contained 30, 31, and 30 participants, respectively. Mean fracture toughness (+ standard deviation) for the placebo, 10% CP, and 15% CP groups were 2.3 ± 0.9, 2.2 ± 0.7, and 2.0 ± 0.5 MPa*m1/2 respectively. There were no significant differences in mean fracture toughness results among the groups (p=0.241). The tooth sensitivity VAS scores indicated a significantly greater incidence (p=0.000) and degree of tooth sensitivity (p=0.049 for VAS change and p=0.003 for max VAS) in the bleach groups than in the placebo group. The color change results showed generally greater color change in the bleach groups than in the placebo group (p=0.008 for shade guide determination and p=0.000 for colorimeter determination). Conclusions There were no significant differences in in situ dentin fracture toughness results among the groups. The results of this study provide some reassurance that dentin is not overtly weakened by the bleaching protocol used in this study. However, the lack of a statistically significant difference cannot be used to state that there is no effect of bleach on dentin fracture toughness.

Materials Sealing Preventing Biofilm Formation in Implant/Abutment Joints: Which Is the Most Effective? A Systematic Review and Meta-Analysis

2020 ◽  
Vol 46 (2) ◽  
pp. 163-171
Author(s):  
Cecília Alves de Sousa ◽  
Maria Beatriz Bello Taborda ◽  
Gustavo Antônio Correa Momesso ◽  
Eduardo Passos Rocha ◽  
Paulo Henrique dos Santos ◽  
...  
Keyword(s):  
Systematic Review ◽  
Biofilm Formation ◽  
Meta Analysis ◽  
Significance Level ◽  
Gutta Percha ◽  
Significant Difference ◽  
Physical Barriers ◽  
External Connection

The purpose of this systematic review was to evaluate the literature available for materials exhibiting the best efficacy in preventing biofilm formation in the interior of implants. We searched PubMed/MEDLINE, Scopus, and Cochrane databases. This review is registered with the PROSPERO database and followed the suitability of the PRISMA protocol. The initial search resulted in 326 articles from the databases. After they were read, 8 articles remained, and the inclusion and exclusion criteria were applied. Six of these 8 articles were classified as in vitro and 2 were classified as in situ. The regions of the implants evaluated ranged from the interface of the pieces to the occlusal upper access of the abutment. The implant connections evaluated the Morse taper, external connection, and internal connection. Meta-analysis of the quantitative data was performed at a significance level of .05. Cotton exhibited poor control of infiltration, even in combination with other materials. Isolated gutta-percha (GP) and polytetrafluoroethylene (PTFE) tape with composite resin (CR) or GP performed better as physical barriers. The best results for chemical barriers were observed by the application of 1% chlorhexidine gluconate (CG) gel, thymol varnish, and the deposition of Ag films onto the surface. The applied meta-analysis did not show a significant difference in comparison between the different types of implant connections (P &gt; .05). The application of CG and thymol varnish antimicrobials was effective in preventing biofilm formation and easy clinical execution; these could be used in combination with CR, GP, and PTFE.

The Erosive Potential of Different Malt Drinks: An in vitro and in situ study

2008 ◽  
Vol 33 (1) ◽  
pp. 35-37 ◽  
Author(s):  
Esber Çaglar ◽  
Sule Kavaloglu Cildir ◽  
Nuket Sandalli
Keyword(s):  
Potential Effect ◽  
Dental Erosion ◽  
Variable Degree ◽  
Water Control ◽  
In Situ Study ◽  
Significant Difference ◽  
Natural Spring ◽  
Erosive Effect

Objectives: Whereas the potential effect of acidic drinks in the etiology of dental erosion is well recognized the role of malt drinks is unclear. The primary aim of the present study was to compare the in vitro erosive effect on enamel produced by different aromated malt drinks. A secondary objective was to compare their erosive effects in situ with those determined in vitro. Materials and methods: To select the malt drink for the study in situ, six commercially available malt drinks were examined for erosive potential in vitro. The study in situ was a single centre, 2-period, 2-treatment crossover study to compare the erosive effect of a commercially available malt drink (Test), with that of natural spring water (Control), over 10 day periods on 10 healthy volunteers. Subjects wore upper removable appliances containing two human enamel specimens from 9 a.m. to 4 p.m. The regimen of intake of the drinks was 250 ml at midday. Measurements of enamel loss were made on samples after 5 and 10 days by profilometry. Results: The in situ study showed a statistically significant difference in erosive potential between the test and control beverages. No specimen exposed to the control beverage displayed appreciable erosion. Erosion occurred with the test drink, but to a variable degree between subjects. Conclusions: Malt drinks should be considered as potentially erosive as the results for enamel specimens exposed to the test beverage in the clinical study showed a degree of erosion that varied greatly between different participants. It is likely that under these conditions an increase in the degree of erosion would be observed in children and young people who consume malt drinks.

scholarly journals CYTOTOXICITY OF IONS RELEASED FROM 17-4 PRECIPITATION HARDENING STAINLESS STEEL ORTHODONTIC BRACKETS IN ARTIFICIAL SALIVA

2018 ◽  
Vol 9 ◽  
pp. 71
Author(s):  
Tjokro Prasetyadi ◽  
Bambang Irawan ◽  
Miesje Karmiati Purwanegara ◽  
Bambang Suharno ◽  
Sugeng Supriadi
Keyword(s):  
Stainless Steel ◽  
Cell Viability ◽  
Precipitation Hardening ◽  
Artificial Saliva ◽  
Nickel Content ◽  
Orthodontic Brackets ◽  
Assay Method ◽  
Cytotoxicity Test ◽  
Significant Difference

Objective: 17-4 precipitation hardening (PH) stainless steel has a low nickel content, which can reduce the risk of allergic reactions. It also has good mechanical properties against the stress caused by the archwire slot brackets in orthodontic treatments. The main focus of this study to evaluate the metal ions released into artificial saliva from different orthodontic brackets with the same 17-4 PH stainless steel and to examine the in vitro cytotoxicity of the metal.Methods: Material properties were analyzed by energy dispersive spectroscopy. The 3-(4,5-dimethylthiazol-2-y1)2,5-diphenyltetrazolium bromide (MTT) assay method was used to examine the cytotoxicity of Gemini and Synergy brackets.Results: The cytotoxicity test on all the orthodontic brackets showed a mean cell viability value above 80% in each immersion group, which means that this material is not cytotoxic to the human immortalized keratinocyte cell line.Conclusions: The results showed cell viability in the extracts of both groups of brackets, and there was no statistically significant difference between the groups (p>0.05).

scholarly journals The In Situ Effect of Titanium Tetrafluoride Gel on Erosion/Abrasion Progression in Human Dentin

2017 ◽  
Vol 28 (3) ◽  
pp. 337-345
Author(s):  
Taís Fonseca Mantilla ◽  
Cecilia Pedroso Turssi ◽  
Thayanne Monteiro Ramos-Oliveira ◽  
Camila Vieira da Silva ◽  
Luis Cláudio Suzuki ◽  
...  
Keyword(s):  
Citric Acid ◽  
Repeated Measures ◽  
Environmental Scanning Electron Microscopy ◽  
Control Groups ◽  
Titanium Tetrafluoride ◽  
Surface Loss ◽  
Significant Difference ◽  
Human Dentin

Abstract Erosion incidence is increasing and its control is still a challenge in clinical practice. This study evaluated 4% TiF4-gel effects on eroded human dentin subjected to in situ erosive/abrasive episodes. Seventy-two previously eroded dentin slabs (0.05 M citric acid, pH 2.3, 20 min) were allocated to 6 groups (n=12) according to the treatment to be performed during the in situ phase and number of erosive/abrasive cycles, as follows: 4% TiF4-gel applied once (TiF41), twice (TiF42) or three times (TiF43) followed by 1, 2 and 3 erosive/abrasive cycles, respectively. Gel was applied before the beginning of the next cycle. Control groups were subjected to 1 (C1), 2 (C2) and 3 (C3) erosive/abrasive cycles only. A seventh group (n=12) comprised in vitro uneroded samples (UN) subjected to 3 erosive/abrasive cycles. Each cycle corresponded to 2 days of erosive (citric acid 0.5%, pH 2.6, 6x/day) and abrasive (electric toothbrush, 10 s/sample, 1 x/day) challenges. Samples were evaluated under profilometry and environmental scanning electron microscopy (ESEM). Atomic force microscopy images (AFM) were also made (n=3). Repeated measures 2-way ANOVA and Tukey test (p<0.001) showed that TiF42, which did not differ from TiF41 and TiF43, revealed a significant reduction in surface loss compared to all control groups. TiF41 and TiF43 showed no significant difference from C1, but both groups demonstrated significantly smaller surface loss than C2 and C3. ESEM and AFM micrographs suggested alterations on treated surfaces compared to samples from control groups, showing reduced diameters of dentinal tubules lumens. Therefore, TiF4 was able to reduce the progression of erosive/abrasive lesions.

Efficacy of Stannous Ions on Enamel Demineralization under Normal and Hyposalivatory Conditions: A Controlled Randomized in situ Pilot Trial

2017 ◽  
Vol 51 (6) ◽  
pp. 543-553 ◽  
Author(s):  
Núbia Inocencya Pavesi Pini ◽  
Nadine Schlueter ◽  
Daniel Sundfeld ◽  
Wiebke Semper Hogg ◽  
Alan Roger Santos-Silva ◽  
...  
Keyword(s):  
Sodium Fluoride ◽  
Pilot Trial ◽  
Salivary Flow ◽  
Factorial Analysis ◽  
Low Flow ◽  
Double Blind ◽  
Significant Difference ◽  
Saliva Flow

The study aim was to investigate the effect of antierosive agents on enamel under normal and hyposalivatory conditions. This double-blind crossover in situ pilot study evaluated 4 toothpastes: placebo (0 ppm F), sodium fluoride (NaF, 1,450 ppm), stannous/sodium fluoride (SnF/NaF, 1,450 ppm F-, 1,090 ppm Sn2+), and sodium fluoride, stannous chloride and chitosan (NaF/Sn/Ch, 1,450 ppm F-, 3,500 ppm Sn2+, 0.5% Ch). Twenty participants were assigned to 2 groups (n = 10 each): normal and low salivary flow. Participants wore palatal appliances holding 4 bovine enamel specimens previously eroded in vitro (D1) for 20 min prior to an in situ phase after which they were eroded again (D2). Surface microhardness was determined at baseline (BL), after D1, in situ phase and D2 to assess hardness loss (%SMH), residual hardness loss (%RHL) and erosion resistance (%RER). Additional specimens were evaluated by scanning electron microscopy after the in situ phase. ANOVA and a factorial analysis for between-subject effects were performed. Sn-based toothpastes showed the best effects (p < 0.05). Under normal flow, SnF/NaF showed higher efficacy, with a significant difference compared to NaF/Sn/Ch, NaF, and placebo (p < 0.05). Under low flow, SnF/NaF and NaF/Sn/Ch were comparable (p > 0.05); NaF and placebo were statistically similar. Comparing salivary conditions, there were significant differences for SnF/NaF for %SMH after the in situ phase (%SMHtotal)), %RHL and for all toothpastes in case of %RER. Factorial analysis revealed interactions between toothpaste and saliva flow for %SMHtotal and %RHL. Salivary flow can influence the efficacy of the antierosive toothpastes; however, Sn2+ preparations show even under low salivary flow conditions the highest efficacy in the prevention of enamel erosion.

Embryos produced in vitro from bulls carrying 16;20 and 1;29 Robertsonian translocations: detection of translocations in embryos by fluorescence in situ hybridization

Zygote ◽  
2005 ◽  
Vol 13 (1) ◽  
pp. 31-34 ◽  
Author(s):  
Roman Rybar ◽  
Jindra Horakova ◽  
Marie Machatkova ◽  
Katerina Hanzalova ◽  
Jiri Rubes
Keyword(s):  
De Novo ◽  
Blastocyst Stage ◽  
Sperm Chromatin ◽  
Tween 20 ◽  
Dna Integrity ◽  
Cell Stage ◽  
Significant Difference ◽  
Painting Probes

Robertsonian translocation rob(16;20) in the heterozygous state was discovered in a subfertile bull of the Czech Siemmental breed. A chromosomal analysis of its family has shown that this dicentric fusion is formed de novo. The present experiments were designed to detect rob(16;20) and determine its incidence for in vitro produced embryos, using fluorescence in situ hybridization (FISH) and rob(1;29) as a detection control. To characterize semen of both bulls with the rob translocations, their sperm was examined for DNA integrity by the sperm chromatin structure assay (SCSA). For in vitro fertilization of oocytes, spermatozoa from a rob(16;20) bull carrier (Czech Siemmental breed) and those from a rob(1;29) bull carrier (Charolais breed) were used. Embryos at the 6- to 8-cell stage were cultured in a vinblastine-supplemented medium for 17 h, and embryos at the blastocyst stage were cultured in a colcemide-supplemented medium for 4 h. The embryos were fixed in methanol and acetic acid with Tween-20. Painting probes for chromosomes 16 (Spectrum Green) and 20 (Spectrum Orange) and chromosomes 1 (Spectrum Orange) and 29 (Spectrum Green) were simultaneously hybridized. In the embryos derived from the rob(16;20) bull, the presence of this translocation was not detected. On the other hand, 52.5% of the embryos derived from the rob(1;29) bull were translocation carriers. There was no significant difference in the frequency of this translocation between early and advanced embryos.

scholarly journals Final Endodontic Irrigation with 2% Peracetic Acid: Antimicrobial Activity and Cytotoxicity

2021 ◽  
Author(s):  
Daniel de Oliveira Brandão-Neto ◽  
João Victor Zebende Mello ◽  
Marilia F Marceliano-Alves ◽  
Thais Machado de Carvalho Coutinho ◽  
Eduardo Fagury Videira Marceliano ◽  
...  
Keyword(s):  
Antimicrobial Activity ◽  
Enterococcus Faecalis ◽  
Peracetic Acid ◽  
Colorimetric Assay ◽  
Multiple Linear Regression Model ◽  
Control Group ◽  
Cytotoxicity Test ◽  
Significant Difference ◽  
Antimicrobial Evaluation

Abstract Objective The aim of present study was to assess the cytotoxicity and antimicrobial efficacy of 2% peracetic acid (PAA) compared with 5.25% sodium hypochlorite (NaOCl) and 2% chlorhexidine (CHX). Material and Methods For the cytotoxicity test, 100 µl of the tested solutions were added in 12 wells with ECV 304 endothelial cells in each group: NaOCl, CHX and PAA, in addition to the control group. Each solution was evaluated after 24 hours of contact in four dilutions: 0.2, 0.1, 0.05 and 0.025 through mitochondrial function using MTT colorimetric assay. In the antimicrobial evaluation, 40 dentin blocks 5 mm in length and 0.2 g in weight were incubated with 400 µl of Enterococcus faecalis suspension for 21 days at 37°C. The contaminated samples were divided into three experimental groups within 5 minutes of contact: NaOCl group, CHX group, PAA group, as well as the positive control group. The specimens received treatment and were transferred to a tube with saline for serial dilution of the solution and seeding for isolation and colony forming unit (CFU) count. Statistical Analysis The results obtained were expressed as mean (A570 nm) ± standard deviation (SD) and in a multiple linear regression model and multiple comparisons conducted. Results The antimicrobial evaluation revealed that the NaOCl and CHX groups showed a statistically significant difference compared with the control group (p < 0.001), while the PAA reduced only the CFU growth. It can be concluded that, among the agents tested, PAA expressed greater cell viability, followed by CHX and NaOCl. However, it did not show greater antimicrobial activity in vitro in the mature biofilm of Enterococcus faecalis.

Antibacterial Effect of Oral Rinses Containing Phytosphingosine

2007 ◽  
Vol 342-343 ◽  
pp. 941-944 ◽  
Author(s):  
S.H. Oh ◽  
M.J. Choi ◽  
B.I. Kim ◽  
Kwang Mahn Kim ◽  
Kyoung Nam Kim
Keyword(s):  
Antibacterial Activity ◽  
Cell Lines ◽  
Apoptotic Cell ◽  
Human Cancer ◽  
Cytotoxicity Test ◽  
Bacterial Strains ◽  
Control Groups ◽  
Oral Rinse ◽  
Significant Difference

The main objective of this study was to manufacture an oral rinse using the natural antibacterial agent (phytosphingosine, Doosan, Korea) for the prevention of periodontal disease and dental caries. Phytosphingosine is known to inhibit the growth of bacterial strains and induce apoptotic cell death in human cancer lines. In this study, antibacterial activity and cytotoxicity of oral rinses were performed with an experimental group containing phytosphingosine(PS) in vitro. Control groups consist of two Korean products and two American products containing chlorhexidine and cetylpyridinium chloride, respectively. There was no significant difference between experimental and control groups in the antibacterial activity and cytotoxicity except for Chika Chika Liq (p<0.05). According to the results, antibacterial activity of oral rinse containing PS was 99.62%, the strongest contact inhibition of Streptococcus mutans strain among tested groups. In the cytotoxicity test of oral rinses, PS had a weaker cytotoxicity than control groups in mouse and human normal cell lines and showed the strongest cytotoxicity in human oral cancer cell lines (KB cell). From the results, PS may be widely used as an oral rinse for the healthy and the patients with oral cancers in the near future.

Sign in / Sign up

Export Citation Format

Share Document