scholarly journals Notoginsenoside R1 Facilitated Wound Healing in High-Fat Diet/Streptozotocin-Induced Diabetic Rats

2022 ◽  
Vol 2022 ◽  
pp. 1-15
Author(s):  
Guangzhao Cao ◽  
Changpei Xiang ◽  
Rui Zhou ◽  
Yi Zhang ◽  
He Xu ◽  
...  
Keyword(s):  
Wound Healing ◽  
High Fat Diet ◽  
Transforming Growth Factor ◽  
Tissue Inhibitor Of Metalloproteinase ◽  
Therapeutic Drug ◽  
High Morbidity ◽  
Diabetic Wound ◽  
High Fat ◽  
Diabetic Wound Healing ◽  
Matrix Metallopeptidase

Diabetic ulcers bring about high morbidity and mortality in patients and cause a great economic burden to society as a whole. Since existing treatments cannot fulfil patient requirements, it is urgent to find effective therapies. In this study, the wound healing effect of topical notoginsenoside R1 (NR1) treatment on diabetic full-thickness wounds in type II diabetes mellitus (T2DM) was induced by the combination of a high-fat diet and streptozotocin (STZ) injection. NR1 significantly increased the wound closure rate, enhanced extracellular matrix (ECM) secretion, promoted collagen growth, increased platelet endothelial cell adhesion molecule-1 (CD31) expression, and decreased cleaved caspase-3 expression. RNA-Seq analysis identified ECM remodeling and inflammation as critical biological processes and Timp1 and Mmp3 as important targets in NR1-mediated wound healing. Further experiments showed that NR1-treated wounds demonstrated higher expression of tissue inhibitor of metalloproteinase 1 (TIMP1) and transforming growth factor-β1 (TGFβ1) and lower expression of matrix metallopeptidase 9 (MMP9), matrix metallopeptidase 3 (MMP3), interleukin-1β (IL-1β), and interleukin-6 (IL-6) than diabetic wounds. These investigations promote the understanding of the mechanism of NR1-mediated diabetic wound healing and provide a promising therapeutic drug to enhance diabetic wound healing.

scholarly journals Nanoparticle-Based Therapeutic Approach for Diabetic Wound Healing

Nanomaterials ◽  
2020 ◽  
Vol 10 (6) ◽  
pp. 1234 ◽  
Author(s):  
Hariharan Ezhilarasu ◽  
Dinesh Vishalli ◽  
S. Thameem Dheen ◽  
Boon-Huat Bay ◽  
Dinesh Kumar Srinivasan
Keyword(s):  
Wound Healing ◽  
High Surface ◽  
Treatment Strategies ◽  
Foot Ulcer ◽  
Volume Ratio ◽  
Therapeutic Drug ◽  
Target Tissue ◽  
Diabetic Wound ◽  
Impaired Wound Healing ◽  
Diabetic Wound Healing

Diabetes mellitus (DM) is a common endocrine disease characterized by a state of hyperglycemia (higher level of glucose in the blood than usual). DM and its complications can lead to diabetic foot ulcer (DFU). DFU is associated with impaired wound healing, due to inappropriate cellular and cytokines response, infection, poor vascularization, and neuropathy. Effective therapeutic strategies for the management of impaired wound could be attained through a better insight of molecular mechanism and pathophysiology of diabetic wound healing. Nanotherapeutics-based agents engineered within 1–100 nm levels, which include nanoparticles and nanoscaffolds, are recent promising treatment strategies for accelerating diabetic wound healing. Nanoparticles are smaller in size and have high surface area to volume ratio that increases the likelihood of biological interaction and penetration at wound site. They are ideal for topical delivery of drugs in a sustained manner, eliciting cell-to-cell interactions, cell proliferation, vascularization, cell signaling, and elaboration of biomolecules necessary for effective wound healing. Furthermore, nanoparticles have the ability to deliver one or more therapeutic drug molecules, such as growth factors, nucleic acids, antibiotics, and antioxidants, which can be released in a sustained manner within the target tissue. This review focuses on recent approaches in the development of nanoparticle-based therapeutics for enhancing diabetic wound healing.

scholarly journals Hypoxia adipose stem cell-derived exosomes promote high-quality healing of diabetic wound involves activation of PI3K/Akt pathways

2021 ◽  
Vol 19 (1) ◽  
Author(s):  
Jie Wang ◽  
Hao Wu ◽  
Yixuan Peng ◽  
Yue Zhao ◽  
Youyou Qin ◽  
...  
Keyword(s):  
Stem Cells ◽  
Wound Healing ◽  
Molecular Mechanisms ◽  
High Throughput Sequencing ◽  
Transforming Growth Factor ◽  
Adipose Stem Cells ◽  
Diabetic Wound ◽  
Skin Wound Healing ◽  
Diabetic Wound Healing ◽  
Diabetic Wounds

AbstractRefractory diabetic wounds can cause persistent inflammation and delayed healing due to hypoxia. Currently, no optimal solution is available. Exosomes of adipose stem cells (ADSCs-exo) may promote skin wound healing, however, molecular mechanisms remains mysterious. We found significantly enhanced survival and proliferation of adipose stem cells after hypoxia induction compared to normoxia. Here, we aimed to investigate if hypoxic adipose stem cells exosomes (HypADSCs-exo) participate in hypoxia adaptability and accelerate diabetic wound healing. Based on high-throughput sequencing, 215 microRNAs (miRNAs) were upregulated and 369 miRNAs downregulated in HypADSCs-exo compared to ADSCs-exo. Up-regulated miR-21-3p, miR-126-5p, miR-31-5p whereas down-regulated gene miR-99b and miR-146-a correlated with wound healing. According to Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG), miRNAs might regulate cell metabolism, differentiation and Transforming growth factor-β (TGF-β) function. Consistently, HpyADSCs-exo could promote diabetic wounds healing and inhibit inflammation through PI3K/AKT signaling pathway. Collectively, HpyADSCs-exo can promote diabetic wound healing as an alternative strategy to improve wound healing.

scholarly journals Berberine accelerated wound healing by restoring TrxR1/JNK in diabetes

2021 ◽  
Vol 135 (4) ◽  
pp. 613-627
Author(s):  
Rui Zhou ◽  
Changpei Xiang ◽  
Guangzhao Cao ◽  
He Xu ◽  
Yi Zhang ◽  
...  
Keyword(s):  
Wound Healing ◽  
Wound Repair ◽  
Transforming Growth Factor ◽  
Cell Model ◽  
Cell Damage ◽  
Redox Homeostasis ◽  
Diabetic Wound ◽  
Closure Rate ◽  
Diabetic Wound Healing ◽  
Diabetic Wounds

Abstract The high disability, mortality and morbidity of diabetic ulcers make it urgent to explore effective strategies for diabetic wound repair. TrxR1 plays a vital role in regulating redox homeostasis in various pathologies. In the present study, the effect of berberine (BBR) on diabetic wounds was investigated in streptozotocin (STZ)-induced diabetic rats and a high glucose (HG)-induced cell model, and the mechanism of BBR on TrxR1 was elucidated. BBR treatment remarkably accelerated wound healing and enhanced extracellular matrix (ECM) synthesis and significantly inhibited HG-induced HaCaT cell damage. Further analysis indicated that BBR activated TrxR1, suppressed its downstream JNK signaling, thereby inhibiting oxidative stress and apoptosis, promoted cell proliferation, down-regulated matrix metalloproteinase (MMP) 9 (MMP9) and up-regulated transforming growth factor-β1 (TGF-β1) and tissue inhibitors of MMP 1 (TIMP1), resulting in accelerated wound healing. Importantly, the enhancement of BBR on wound repair was further abolished by TrxR1 inhibitor. Moreover, in diabetic wounds induced by a combination of STZ injection and high-fat diet, BBR significantly increased wound closure rate and TrxR1 expression, and this was reversed by TrxR1 inhibitor. These data indicated that topical BBR treatment accelerated diabetic wound healing by activating TrxR1. Targeting TrxR1 may be a novel, effective strategy for restoring redox homeostasis and promoting diabetic wound healing.

The diabetic wound healing effect of a two-herb formula and its mechanisms of action

Planta Medica ◽  
2012 ◽  
Vol 78 (11) ◽  
Author(s):  
CBS Lau ◽  
VKM Lau ◽  
CL Liu ◽  
PKK Lai ◽  
JCW Tam ◽  
...  
Keyword(s):  
Wound Healing ◽  
Mechanisms Of Action ◽  
Diabetic Wound ◽  
Healing Effect ◽  
Diabetic Wound Healing ◽  
Wound Healing Effect

Critical Regulation of Angiogenesis by Nrf2 Signaling Is Absent in Diabetic Wound Healing

Diabetes ◽  
2018 ◽  
Vol 67 (Supplement 1) ◽  
pp. 634-P
Author(s):  
PIUL S. RABBANI ◽  
JOSHUA A. DAVID ◽  
DARREN L. SULTAN ◽  
ALVARO P. VILLARREAL-PONCE ◽  
JENNIFER KWONG ◽  
...  
Keyword(s):  
Wound Healing ◽  
Diabetic Wound ◽  
Diabetic Wound Healing

A bioglass sustained-release scaffold with ECM-like structure for enhanced diabetic wound healing

Nanomedicine ◽  
2020 ◽  
Vol 15 (23) ◽  
pp. 2241-2253
Author(s):  
Pengju Zhang ◽  
Yuqi Jiang ◽  
Dan Liu ◽  
Yan Liu ◽  
Qinfei Ke ◽  
...  
Keyword(s):  
Wound Healing ◽  
Therapeutic Option ◽  
Effective Strategy ◽  
Diabetic Wound ◽  
Nano Structure ◽  
Wound Site ◽  
Diabetic Wound Healing ◽  
Coaxial Fibers ◽  
Diabetic Wounds ◽  
Endothelial Cell Adhesion

Aim: To develop an effective strategy for increasing angiogenesis at diabetic wound sites and thereby accelerating wound healing. Materials & methods: A micropatterned nanofibrous scaffold with bioglass nanoparticles encapsulated inside coaxial fibers was prepared by electrospinning. Results: Si ions could be released in a sustained manner from the scaffolds. The hierarchical micro-/nano-structure of the scaffold was found to act as a temporary extracellular matrix to promote endothelial cell adhesion and growth. The scaffold greatly improved angiogenesis and collagen deposition at the wound site, which shortened the healing period of diabetic wounds. Conclusion: This study provides a promising therapeutic option for chronic diabetic wounds with improved angiogenesis.

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